WO2002054044A3 - Focused acoustic ejection cell sorting system and method - Google Patents

Focused acoustic ejection cell sorting system and method Download PDF

Info

Publication number
WO2002054044A3
WO2002054044A3 PCT/US2001/050753 US0150753W WO02054044A3 WO 2002054044 A3 WO2002054044 A3 WO 2002054044A3 US 0150753 W US0150753 W US 0150753W WO 02054044 A3 WO02054044 A3 WO 02054044A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
volumes
ejected
sorting
array
Prior art date
Application number
PCT/US2001/050753
Other languages
French (fr)
Other versions
WO2002054044A2 (en
Inventor
Mitchell W Mutz
Richard N Ellson
David Soong-Hua Lee
Original Assignee
Picoliter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Picoliter Inc filed Critical Picoliter Inc
Priority to ES01991599.0T priority Critical patent/ES2551890T3/en
Priority to EP10180763.4A priority patent/EP2267429B1/en
Priority to CA2433296A priority patent/CA2433296C/en
Priority to AU2002231324A priority patent/AU2002231324A1/en
Priority to DK01991599.0T priority patent/DK1348116T3/en
Priority to EP01991599.0A priority patent/EP1348116B1/en
Priority to JP2002554696A priority patent/JP4309131B2/en
Publication of WO2002054044A2 publication Critical patent/WO2002054044A2/en
Publication of WO2002054044A3 publication Critical patent/WO2002054044A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
    • B41J2/00—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed
    • B41J2/005—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by bringing liquid or particles selectively into contact with a printing material
    • B41J2/01—Ink jet
    • B41J2/135—Nozzles
    • B41J2/14—Structure thereof only for on-demand ink jet heads
    • B41J2/14008—Structure of acoustic ink jet print heads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G01N15/1023—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/02—Analysing fluids
    • G01N29/028—Analysing fluids by measuring mechanical or acoustic impedance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
    • G01N15/149—
    • G01N2015/1028—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/1415—Control of particle position
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/142—Acoustic or ultrasonic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1039—Micropipettes, e.g. microcapillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1062—General features of the devices using the transfer device for another function for testing the liquid while it is in the transfer device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Abstract

A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.
PCT/US2001/050753 2000-12-28 2001-12-28 Focused acoustic ejection cell sorting system and method WO2002054044A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
ES01991599.0T ES2551890T3 (en) 2000-12-28 2001-12-28 Method and system of cellular classification by focused acoustic expulsion
EP10180763.4A EP2267429B1 (en) 2000-12-28 2001-12-28 Focused acoustic ejection cell sorting system and method
CA2433296A CA2433296C (en) 2000-12-28 2001-12-28 Focused acoustic ejection cell sorting system and method
AU2002231324A AU2002231324A1 (en) 2000-12-28 2001-12-28 Focused acoustic ejection cell sorting system and method
DK01991599.0T DK1348116T3 (en) 2000-12-28 2001-12-28 System for sorting cells by focused acoustic ejection, and method thereof
EP01991599.0A EP1348116B1 (en) 2000-12-28 2001-12-28 Focused acoustic ejection cell sorting system and method
JP2002554696A JP4309131B2 (en) 2000-12-28 2001-12-28 Focused acoustic discharge cell sorting system and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/751,666 2000-12-28
US09/751,666 US20020064809A1 (en) 2000-11-29 2000-12-28 Focused acoustic ejection cell sorting system and method

Publications (2)

Publication Number Publication Date
WO2002054044A2 WO2002054044A2 (en) 2002-07-11
WO2002054044A3 true WO2002054044A3 (en) 2003-06-12

Family

ID=25022973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050753 WO2002054044A2 (en) 2000-12-28 2001-12-28 Focused acoustic ejection cell sorting system and method

Country Status (8)

Country Link
US (1) US20020064809A1 (en)
EP (2) EP1348116B1 (en)
JP (1) JP4309131B2 (en)
AU (1) AU2002231324A1 (en)
CA (1) CA2433296C (en)
DK (1) DK1348116T3 (en)
ES (1) ES2551890T3 (en)
WO (1) WO2002054044A2 (en)

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Also Published As

Publication number Publication date
EP2267429A1 (en) 2010-12-29
WO2002054044A2 (en) 2002-07-11
JP4309131B2 (en) 2009-08-05
CA2433296A1 (en) 2002-07-11
EP2267429B1 (en) 2019-07-17
JP2004530863A (en) 2004-10-07
US20020064809A1 (en) 2002-05-30
CA2433296C (en) 2013-10-01
EP1348116B1 (en) 2015-09-02
EP1348116A2 (en) 2003-10-01
AU2002231324A1 (en) 2002-07-16
ES2551890T3 (en) 2015-11-24
DK1348116T3 (en) 2015-12-07

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