WO2002057493A3 - Methods for determining organisms - Google Patents

Methods for determining organisms Download PDF

Info

Publication number
WO2002057493A3
WO2002057493A3 PCT/US2001/048675 US0148675W WO02057493A3 WO 2002057493 A3 WO2002057493 A3 WO 2002057493A3 US 0148675 W US0148675 W US 0148675W WO 02057493 A3 WO02057493 A3 WO 02057493A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
methods
determining
cells
determination
Prior art date
Application number
PCT/US2001/048675
Other languages
French (fr)
Other versions
WO2002057493A2 (en
Inventor
Susan Rigby
Heather P O'keefe
Henrik Stender
Original Assignee
Boston Probes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston Probes Inc filed Critical Boston Probes Inc
Priority to EP01994259A priority Critical patent/EP1356113B1/en
Priority to AU2002246676A priority patent/AU2002246676A1/en
Publication of WO2002057493A2 publication Critical patent/WO2002057493A2/en
Publication of WO2002057493A3 publication Critical patent/WO2002057493A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Abstract

This invention is directed to a rapid and simple method for determining organisms and/or cells in a sample. Generally, the method is directed to the use of molecular probes to selectively stain the organisms and/or cells for determination wherein growth medium, fixative reagents and/or excess molecular probes need not be separated before a determination is made.
PCT/US2001/048675 2000-12-15 2001-12-14 Methods for determining organisms WO2002057493A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01994259A EP1356113B1 (en) 2000-12-15 2001-12-14 Methods for determining organisms
AU2002246676A AU2002246676A1 (en) 2000-12-15 2001-12-14 Methods for determining organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25614000P 2000-12-15 2000-12-15
US60/256,140 2000-12-15

Publications (2)

Publication Number Publication Date
WO2002057493A2 WO2002057493A2 (en) 2002-07-25
WO2002057493A3 true WO2002057493A3 (en) 2003-09-04

Family

ID=22971223

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/048675 WO2002057493A2 (en) 2000-12-15 2001-12-14 Methods for determining organisms

Country Status (4)

Country Link
US (1) US6905824B2 (en)
EP (1) EP1356113B1 (en)
AU (1) AU2002246676A1 (en)
WO (1) WO2002057493A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017758B2 (en) * 2002-03-21 2011-09-13 Boston Probes, Inc. PNA oligomers, oligomer sets, methods and kits pertaining to the detection of Bacillus anthracis
WO2003100076A2 (en) * 2002-05-17 2003-12-04 Applera Corporation Pna probes, probe sets, method and kits pertaining to the determination of listeria
WO2005018423A2 (en) * 2003-08-14 2005-03-03 Applera Corporation Pna oligomers, oligomer sets, methods and kits pertaining to the determination of enterococcus faecalis and other enterococcus species
WO2005121373A2 (en) * 2004-06-03 2005-12-22 Advandx, Inc. Hybridization of pna probes in alcohol solutions
US20140080726A1 (en) * 2012-08-01 2014-03-20 Nanomdx, Inc. Enhanced method for probe based detection of nucleic acids
US20140178875A1 (en) 2012-10-09 2014-06-26 Advandx, Inc. Devices, Compositions and Methods Pertaining To Microscopic Analysis Of Microorganisms and Other Analytes of Interest
DE102020103957B4 (en) * 2020-02-14 2022-01-13 Testo bioAnalytics GmbH Method for detecting microorganisms and a fluidic channel system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591570A (en) * 1983-02-02 1986-05-27 Centocor, Inc. Matrix of antibody-coated spots for determination of antigens

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10910A (en) * 1854-05-16 Island
US5225326A (en) * 1988-08-31 1993-07-06 Research Development Foundation One step in situ hybridization assay
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5714331A (en) * 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5766855A (en) * 1991-05-24 1998-06-16 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity and sequence specificity
US5641625A (en) * 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
US5719262A (en) * 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5527675A (en) * 1993-08-20 1996-06-18 Millipore Corporation Method for degradation and sequencing of polymers which sequentially eliminate terminal residues
DE4331012A1 (en) * 1993-09-13 1995-03-16 Bayer Ag Nucleic acid-binding oligomers with N-branching for therapy and diagnostics
GB2284209A (en) * 1993-11-25 1995-05-31 Ole Buchardt Nucleic acid analogue-induced transcription of RNA from a double-stranded DNA template
JP2001518054A (en) 1995-06-07 2001-10-09 パーセプティブ バイオシステムズ，インコーポレーテッド PNA-DNA chimera and PNA synthon for chimera synthesis
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
CA2218439A1 (en) 1996-12-21 1998-06-21 Henrik Orum Method of identifying a nucleic acid using triple helix formation of adjacently annealed probes
US6107470A (en) * 1997-05-29 2000-08-22 Nielsen; Peter E. Histidine-containing peptide nucleic acids
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6326479B1 (en) 1998-01-27 2001-12-04 Boston Probes, Inc. Synthetic polymers and methods, kits or compositions for modulating the solubility of same
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US6280946B2 (en) * 1998-08-07 2001-08-28 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the universal detection of bacteria and eucarya
US6403309B1 (en) * 1999-03-19 2002-06-11 Valigen (Us), Inc. Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591570A (en) * 1983-02-02 1986-05-27 Centocor, Inc. Matrix of antibody-coated spots for determination of antigens

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
LUK J M C ET AL: "RAPID AND SENSITIVE DETECTION OF SALMONELLA (O: 6,7) BY IMMUNOMAGNETIC MONOCLONAL ANTIBODY-BASED ASSAYS", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 137, no. 1, 1991, pages 1 - 8, XP000566449, ISSN: 0022-1759 *
PERRY-O'KEEFE HEATHER ET AL: "Identification of indicator microorganisms using a standardized PNA FISH method.", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 47, no. 3, December 2001 (2001-12-01), pages 281 - 292, XP002228739, ISSN: 0167-7012 *
PLUSKAL M ET AL: "PEPTIDE NUCLEIC ACID PROBES AND THEIR APPLICATION IN DNA AND RNA BLOT HYBRIDIZATION ANALYSIS", FASEB JOURNAL, FED. OF AMERICAN SOC. FOR EXPERIMENTAL BIOLOGY, BETHESDA, MD, US, vol. 8, no. 7, 21 May 1994 (1994-05-21), pages A1264, XP000601669, ISSN: 0892-6638 *
STENDER H ET AL: "I-124. SIMULTANEOUS IDENTIFICATION AND ENUMERATION OF MICROORGANISMS BY FILTER-BASED IN SITU HYBRIDIZATION USING ENZYME-LABELED PEPTIDE NUCLEIC ACID PROBES", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 100, 21 May 2000 (2000-05-21), pages 408, XP000956166, ISSN: 0067-2777 *
STENDER H ET AL: "P-26. A NEW MOLECULAR METHOD FOR SIMULTANEOUS IDENTIFICATION AND ENUMERATION OF BRETTANOMYCES IN WINE", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 99, 31 May 1999 (1999-05-31), pages 516, XP000952544, ISSN: 0067-2777 *
STENDER HENRIK ET AL: "Combination of ATP-bioluminescence and PNA probes allows rapid total counts and identification of specific microorganisms in mixed populations.", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 46, no. 1, 2001, pages 69 - 75, XP002228740, ISSN: 0167-7012 *
STENDER HENRIK ET AL: "Rapid detection, identification, and enumeration of Escherichia coli by fluorescence in situ hybridization using an array scanner.", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 45, no. 1, May 2001 (2001-05-01), pages 31 - 39, XP002228741, ISSN: 0167-7012 *
STENDER HENRIK ET AL: "Rapid detection, identification, and enumeration of Pseudomonas aeruginosa in bottled water using peptide nucleic acid probes.", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 42, no. 3, November 2000 (2000-11-01), pages 245 - 253, XP002228742, ISSN: 0167-7012 *

Also Published As

Publication number Publication date
US6905824B2 (en) 2005-06-14
EP1356113B1 (en) 2012-07-18
US20030165856A1 (en) 2003-09-04
EP1356113A2 (en) 2003-10-29
AU2002246676A1 (en) 2002-07-30
WO2002057493A2 (en) 2002-07-25

Similar Documents

Publication Publication Date Title
WO2002002811A3 (en) Method for controlling the microbiological quality of an aqueous medium and kit therefor
WO2002077604A3 (en) Labeled cells for use as an internal functional control in rare cell detection assays
WO1999042839A3 (en) Treating cancer
WO2002040998A3 (en) Kit and method for determining multiple analytes
WO2006020579A3 (en) Method for rapid identification of microorganisms
ATE258683T1 (en) METHOD FOR PERFORMING SALIVA ANALYSIS
WO2000001845A3 (en) Method for early diagnosis of carcinomas
AU1725200A (en) Simultaneous determination of equilibrium and kinetic properties
AU2001288032A1 (en) Method and kit for the transderaml determination of analyte concentration in blood
ATE466930T1 (en) UNIVERSAL COLLECTION MEDIA
EP1104772A4 (en) Antibody for detecting microorganism
WO2002008461A3 (en) A METHOD AND AN ALGORITHM FOR mRNA EXPRESSION ANALYSIS
WO2003050291A3 (en) Sensor for the use in testing biological chemical or environmental samples
EP1310793A4 (en) Substrate activation kit and method of detecting dna or the like by using the same
EP0982592A3 (en) Improved method of determining the amount of estradiol in a fluid sample and a respective kit therefor
WO2002057493A3 (en) Methods for determining organisms
WO2000075363A3 (en) Enzymatic measurement of mycophenolic acid
WO2001086285A3 (en) Direct detection of individual molecules
WO2002014535A3 (en) Electrochemical strip test for small volumes
CA2407760A1 (en) Methods for diagnosis of low grade astrocytoma
WO2004077057A8 (en) Standard for immunohistochemistry, immunocytochemistry and molecular cytogenetics
WO1998011250A3 (en) Detection of microorganisms
WO2004043227A3 (en) Polynucleotides for the amplification and detection of chlamydia trachomatis and neisseria gonorrhoeae
WO1997046709A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(CHLAMYDIA PNEUMONIAE)
WO2002044733A3 (en) Methods and compositions for sorting and/or determining microorganisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2001994259

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001994259

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP