WOUND DRESSING
This invention relates to the dressing of wounds; more particularly, the present invention relates to the dressing of chronic wounds using materials not customarily regarded as pharmaceutical agents.
It is known that, in chronic wounds, a large number of endogenously produced factors are released into the wound. It is believed by some workers in this field that the abnormalities of tissue repair demonstrated in chronic wounds may, at least in part, be due to the over-activity of tissue degradation mechanisms mediated by some of these factors.
In particular, there is evidence to suggest that proteolytic enzymes may be implicated in the pathogenesis of chronic wounds. Thus, it is believed that the presence of proteases produced in the wound may result in extensive extra-cellular matrix degradation to dermal components such as collagens and proteoglycans.
This invention seeks to reduce the effect of at least certain proteases in frustrating the healing of chronic wounds.
According, therefore, to a first aspect of this invention, there is provided a water-absorbent, composite material which comprises:
i) at least 30%, by weight of the composite material, of an alginate in admixture with: ii) a hydrogel-forming, synthetic, non-polysaccharide polymer; and/or iii) a mixture of at least two hydrogel-forming polymers different from ii) .
In the composite material of the invention the alginate may comprise at least 40%, preferably at least 50%, by weight of the composite material. Suitably, the alginate may have been rendered insoluble. Desirably, the alginate may comprise a major amount of mannuronic (high-M) moieties.
Component ii) of the composite material of the invention may comprise homo- or copolymer of N-vinyl pyrrolidone, acrylamide or ethylene oxide. One of the components iii) may comprise a semi-synthetic derivative of cellulose. One of the components iii) may comprise cellulose, hyaluronic acid or a pectin. Component iii) may comprise a mixture of two or more such components or at least one such component with a semi-synthetic derivative of cellulose.
The composite material of the invention suitably may have at least one of the components of i) , ii) and/or iii) in a fibrous form; for example wherein at least two of the components are preparable by co-spinning. The material may be felted. The material may be woven or knitted.
This invention also provides a wound dressing which comprises a composite material as herein disclosed.
According to a further aspect of this invention there is provided use of a composite material or polymer which is swellable in aqueous media for the manufacture of a wound dressing comprising the composite material or polymer to reduce the concentration of proteolytic enzyme in a wound by application of the wound dressing externally thereto.
Preferably in such use, the composite material is as herein defined. Thus the polymer may be a substituted or unsubstituted, homo- or co- polysaccharide. Suitably, the polymer may comprise uronic acid groups.
Desirably, the substituted polysaccharide may comprise etherified or
acylated hydroxyl groups, and/or may comprise esterified uronic groups. In particular, the substituted polysaccharide may comprise at least some hydroxyl groups which have been replaced by amino or acylated amino groups.
The aforementioned substituent may comprise a saturated or unsaturated, carbocyclic or heterocyclic, mono- or polycyclic group. The unsaturated group may include an aromatic group. Suitably, the polycyclic group may comprise a fused polycyclic structure. The polymer may be cross-linked. It may be formed as a film or as a fibre. Furthermore, a mixture of polymers as herein defined may be used in the manufacture of the wound dressing. When the polymer, or at least a component in a mixture of polymers, is formed as a fibre, the fibres may be disposed in the wound dressing manufactured therefrom as a non- woven mat or as a woven fibre. The materials in the wound dressing manufactured therefrom may be associated with one or more non- water swellable materials.
This invention also provides the use of a polymer as herein defined as a wound dressing agent.
This invention further provides a method of treatment of the human body, which method comprises applying externally to a wound on the body a wound dressing which comprises a composite material or a polymer which is swellable in aqueous media, the application thereby reducing the concentration of proteolytic enzymes in the wound. Desirably, the composite material or the polymer is herein defined.
While it is intended that the wound dressings in accordance with the present invention may be used without preservations or pharmacologically active ingredients, it is possible to include these in minor amount. For example, an antibiotic or anti-microbial agent such as metronidazole,
silver sulphadiazine, neomycin or penicillin; antiseptic agents such as povidone iodine; anti-inflammatory agents such as hydrocortisone or triamcinolone acteonide; or skin protective agents such as zinc oxide may be included.
The following Examples illustrate the invention in which reference is made to the following figures:
Figure 1 shows the % elastase activity remaining in solution after incubation for 3 hours with AQUACEL®. The control was incubated without AQUACEL®.
Figure 2.1 shows the % elastase activity remaining in solution after incubation for 3 hours with alginate (Kaltostat®, ConvaTec) . The control was incubated without alginate.
Figure 2.2 shows the amount of elastase activity that is recovered in the washing supernatant is significantly lower in Kaltostat® than the comparator dressing materials.
Figure 3 shows the % elastase activity remaining in solution after incubation for 3 hours with high-M alginate (Kaltogel™,
ConvaTec) .
Figure 4.1 shows the % elastase activity remaining in solution after incubation for 1 hour with various alginate-containing preparations.
Figure 4.2 shows the amount of elastase activity that is recovered in the washing supernatant in formulation AC6040 is negligible and significantly lower in comparison to pure preparations of alginate (A100) and pure sodium CMC (C100), which are in turn lower than the comparator gauze dressing and
Allevyn™.
Figure 5 shows the amount of elastase activity that is recovered in the washing supernatant in formulations ACL404020 and AC6040 (Example 4) is negligible and significantly lower in comparison to pure preparations of alginate (A100) and pure sodium CMC (C100) , which are in turn lower than the comparator gauze dressings and Allevyn™.
Figure 6.1 shows the % elastase activity remaining in solution after incubation for 1 hour with various alginate-containing preparations.
Figure 6.2 shows the amount of elastase activity that is recovered in the washing supernatant of Alginate/PNP composition is comparable to those of the comparator dressing materials, in this case two gauze materials (standard surgical gauze and ΝA™ gauze) and a highly absorbent commercially-available surgical dressing (Allevyn™) .
Figure 7.1 shows the % elastase activity remaining in solution after incubation for 1 hour with various preparations. All incubations were carried out with the same incubation volume to material weight ratio.
Figure 7.2 shows the amount of elastase activity that is recovered in the washing supernatant of Alginate/CMC/HYAFF composition is comparable to those of the comparator dressing materials.
Figure 8.1 shows the % elastase activity remaining in solution after incubation for 1 hour with the various preparations.
Figure 8.2 shows the amount of elastase activity that is recovered in the washing supernatant of Alginate/CMC/pectin composition is comparable to those of the comparator dressing materials.
Figure 9.1 shows % elastase activity remaining in solution after incubation for 1 hour with the various preparations.
Figure 9.2 shows the amount of elastase activity that is recovered in the washing supernatant of Alginate/PEO composition is comparable to those of the comparator dressing materials.
Figure 10.1 shows the % elastase activity remaining in solution after incubation for 1 hour with the various preparations.
Figure 10.2 shows that the amount of elastase activity that is recovered in the washing supernatant of pectin mixture composition is comparable to the comparator dressing materials.
Figure 11.1 shows the % elastase activity remaining in solution after incubation for 1 hour with the various preparations.
Figure 11.2 shows the amount of elastase activity that is recovered in the washing supernatant of pectin/CMC composition is less than those of the comparator dressing material.
EXAMPLE 1
Pure sodium carboxymethyl cellulose (CMC) fibres (AQUACEL®, ex ConvaTec) gel upon hydration, the water being absorbed into the fibres. In experiments where AQUACEL® is soaked in an excess of fluid containing elastase, AQUACEL® is shown to concentrate the elastase in the supernatant, indicating absorption of water in preference to proteinaceous substances in solution. However, this test methodology is not directly applicable to clinical conditions where dressings are normally applied in excess of wound exudate. In an alternative approach, solutions containing elastase were applied to test materials comprising both AQUACEL® and also to comparator dressing materials so that the solutions were entirely soaked into the dressing without leaving any supernatant. These dressing materials were then washed and the amount of elastase detected in the wash solution gave an indication of how much elastase was retained in the dressing material.
Elastase solution from a bacterial source Pseudomouas aeruginosa (100 μl aliquots, 2.34 μU/ml) was applied to 1 cm2 square pieces of each of the test materials. The solution was allowed to soak in for five minutes. The material was then washed with 1 ml of phosphate buffered saline for 1 hour at room temperature. Aliquots of the supernatant were next withdrawn to assay for elastase activity by an azocasein method whereby the hydrolysed azocasein releases a soluble coloured product that can be measured by standard spectiophotometric methodology. The comparator dressing materials were two gauze materials (standard surgical gauze and
NA™ gauze ex Johnson + Johnson) and a highly absorbent commercially available surgical dressing (Allevyn™ ex Smith + Nephew) .
Results demonstrate that the amount of elastase activity that was recovered in the washing supernatant was significantly lower in AQUACEL® than the comparator dressing materials. Figure 1 shows the % elastase activity remaining in solution after incubation for 3 hours with AQUACEL® (column B) . The control, column A, shows incubation without AQUACEL®.
It is believed, though not ascertained, that AQUACEL® forms a cohesive gel upon hydration. Initial uptake of the proteinase solution into AQUACEL® is likely to be by capillary absorption. Water is then absorbed into the CMC fibres with a consequent closing of the inter-fibre space, trapping the proteins there. The protein is difficult to wash out thereafter because of its physical entrapment.
AQUACEL®, NA™ and ALLEVYN® are trade marks.
EXAMPLE 2
Calcium alginate (Kaltostat® ex ConvaTec) is found to have the ability to absorb elastase in vitro.
The test materials were cut to provide a small amount of material (5 mg) which was then incubated at room temperature with 1.5 ml of the elastase solution containing 50 μU/ml. The elastase solution consisted of purified human neutrophil elastase (Calbiochem) in 50 mM Tris-HCl and 0.05% triton X-100 buffer at pH 8.0. At the end of the incubation period of 3 hours, a small aliquot of the supernatant was reacted with Elastase Substrate 1 (a calorimetric substrate: Calbiochem) in a Cobas Fara II auto-analyser to determine the activity concentration of elastase by comparison with an enzyme standard. Three tests were run together with a control in which incubation was undertaken without alginate being
present. All tests with alginate were carried out with the same incubation volume to alginate weight ratio.
Figure 2.1 shows the results in which the elastase solution was incubated with alginate for three hours. The control was incubated without alginate. All incubations with alginate were carried out with the same incubation volume to alginate weight ratio as described above. The data from three independent experiments are shown.
It will be seen that the enzyme activity remaining in solution was considerably reduced in comparison to the control in which the enzyme was incubated in identical conditions but without alginate.
Alginate fibrous material (Kaltostat®) was also found to have a better ability than gauzes to retain proteinases that were soaked into the dressing. To study this attribute, the procedure of Example 1 was followed but replacing the AQUACEL® with Kaltostat®. Figure 2.2 shows that the amount of elastase activity that was recovered in the washing supernatant was significantly lower in Kaltostat® (column D) than comparator dressing materials. These were a standard surgical gauze (column A) , NA™ gauze (column B) and a highly absorbent commercially- available surgical dressing (Allevyn™) (column C) .
KALTOSTAT® is a trade mark.
EXAMPLE 3
Alginate with a high mannuronic acid content (high-M alginate) has a different gelling characteristic from that of high guluronic content alginate (high-G content) (such as that used in Example 2) upon hydration.
The test materials were cut to provide a small amount of material (5 mg) which was then tested as in Example 2.
The results, shown in Figure 3, demonstrate that the enzyme activity remaining in solution was considerably reduced in comparison to the control in which the enzyme was incubated in identical conditions but without the high-M alginate. All incubations with high-M alginate were carried out with the same incubation volume to alginagte weight ratio as described in Example 2.
EXAMPLE 4
A composite fibrous material containing 45% alginate and 55% NaCMC was produced in accordance with Example 1 of International Patent Publication WO 97/39170.
This composite fibrous material was then tested as in Example 2 but for an incubation period of one hour only. Like tests were also made with AQUACEL® and with Kaltostat®. All tests with alginate were carried out with the same incubation volume to alginate weight ratio.
The results, shown in Figure 4.1 , demonstrate that when this composite material is incubated with an elastase solution for one hour, the residual elastase activity in solution is considerably reduced (column C) beyond that achieved by incubation with the same weight of alginate alone (column B) , or with the same weight of NaCMC alone (column D) . The control (column A) was incubated without added material.
An additional mixed fibre composition containing alginate and sodium CMC in the ratios of 60%:40% (AC6040) was also produced in
accordance with Example 1 of International Patent Publication WO 97/39170. This composite fibrous material was tested as in Example 1. The comparator dressing materials were two gauze materials (standard surgical gauze and NA™ gauze) ; a highly absorbent commercially available surgical dressing (Allevyn™) ; pure alginate (A100) and; pure sodium CMC (C100) .
The results, shown in Figure 4.2, demonstrate that the amount of elastase activity that was recovered in the washing supernatant in formulation AC6040 was negligible and significantly lower in comparison to pure preparations of alginate (A100) and pure sodium CMC (C100) . This shows an improved ability to retain the elastase in the fibres. The ability of the pure preparations to retain elastase was, in turn, superior to that of the comparator gauze dressing materials and Allevyn™. The combined data shown in Figures 4.1 and 4.2 indicate that the composite materials consisting of alginate and sodium CMC, with alginate at or in excess of 40% of the total fibre content, had superior proteinase absorption and retention capability.
In pathological conditions where proteinases are known to be a cause of tissue damage, these alginate-sodium CMC compositions may contribute to reducing the concentration of free proteinase by effective sequestration of these enzymes, and thereby contribute to reducing tissue breakdown. This is likely to be particularly appropriate in chronic wound conditions.
EXAMPLE 5
Mixed fibre compositions containing alginate and sodium CMC in the ratios of 30%:70% (AC3070) , 50%:50% (AC5050) and 60%:40% (AC6040) were produced in accordance with Example 1 of International
Patent Publication WO 97/39170. These mixed fibre compositions were
additionally mixed with cellulosic fibres to form non-woven fabrics using a standard needled non-woven process with the following compositions:
The results, shown in Figure 5, demonstrate that the amount of elastase activity that is recovered in the washing supernatant in formulations ACL404020 and AC 6040 (Example 4) is negligible and significantly lower in comparison to pure preparations of alginate (A100) and pure sodium CMC (CIOO), showing improved ability to retain elastase in the fibres. The ability of the pure preparations to retain elastase was, in turn, superior to that of the comparator gauze dressing materials and Allevyn™. In both of these materials, the amount, by weight, of alginate is 40% or more.
In pathological conditions where proteinases are known to be a cause of tissue damage, these alginate-sodium CMC compositions with or without
added viscose (Lyocell™) may contribute to reducing the concentration of free proteinase by effective sequestration of these enzymes, and thereby contribute to reducing tissue breakdown. This is likely to be particularly appropriate in chronic wound conditions.
LYOCELL™ is a trade mark.
EXAMPLE 6
A composite fibrous material containing alginate and poly(N- vinylpyrrolidone) (PNP) in the ratio of 1: 1 was produced as follows. Alginate/PNP fibre samples were spun from a dope solution containing 6% w/w of solids comprising a 50/50 mixture of sodium alginate and PNP in water. The dope solution was made by adding 120g of PNP (ex Aldrich, 10,000 Da MW) and 120g of sodium alginate (ex Kelco LF10 10/60D) to 3790g of deionised water. The mixture was stirred with a high-speed mixer until the ingredients had dissolved and the dope allowed to stand overnight to degas.
The degassed dope was then poured into a pressure vessel attached to a spinning rig. The dope was next pumped to the 400 jet spinneret of the spinning rig and the rig started. The spinneret was immersed in the spin bath of the rig, which contained a solution of 0.3 mol/dm3 of calcium chloride. The fibres so produced were threaded over three rollers of the first godet and then over the rollers of a second godet. The resulting yarn was then passed through two baths of propan-2-ol, which were maintained at a concentration to dry the yarn. The yarn was then passed through a set of pinch rollers and wound onto cones.
Figure 6.1 shows that when this composite material is incubated with an elastase solution for one hour as in Example 4, the residual elastase
activity in solution is considerably reduced beyond that achieved by incubation with the same weight of alginate alone. As before, all incubations with alginate were carried out with the same incubation volume to material weight ratio. The alginate was Kaltostat® wound dressing ex ConvaTec.
The procedure of Example 1 was next repeated but replacing the AQUACEL® with the alginate: PNP mixture and with Kaltostat®. Figure 6.2 shows that the amount of elastase activity that was recovered in the washing supernatant was comparable to the comparator dressing materials, in this case two gauze materials (standard surgical gauze and ΝA™ gauze) and a highly absorbent commercially available surgical dressing (Allevyn™) . When taking into account the proteinase absorptive capability as shown in Figure 6.1 , this composition may also have a usefulness in modulating proteinase levels in the wound environment.
EXAMPLE 7
A composite fibrous material containing 42.5% alginate, 52.5% ΝaCMC and 5% HYAFF (a benzyl ester of hyaluronic acid) was spun from a dope solution containing 6% w/w of solids in water. The dope solution was made by adding 102g of sodium alginate (ex Kelco LF 10/60D) , 126g of ΝaCMC (ex Hercules Blanose 12MSP) and 12g of HYAFF powder (ex Fidia Biopolymers) to 3760g of deionised water. The mixture was stirred with a high-speed mixer until the ingredients had dissolved and the dope allowed to stand overnight to degas. It was then spun as in Example 6. It was then tested in the same manner.
Figure 7.1 shows that the alginate/CMC/HYAFF composite material has the ability to absorb elastase substantially better than that of insoluble alginates (Kaltostat®) and carboxymethyl cellulose (AQUACEL®) .
Figure 7.2 shows that the amount of elastase activity that was recovered in the washing supernatant was comparable to the comparator dressing materials, in this case two gauze materials (standard surgical gauze and NA™ gauze) and a highly absorbent commercially-available surgical dressing (Allevyn™) . When taking into the account the proteinase absorptive capability as shown in Figure 7.1, the composition may also have a usefulness in modulating proteinase levels in the wound environment.
EXAMPLE 8
A composite fibrous material containing 30% alginate, 60%NaCMC and 10% Pectin was spun from a dope solution containing 6% w/w of solids in water. The dope solution was made by adding 72g of sodium alginate (ex Kelco LF 10/60D) , 144g of NaCMC (ex Hercules Blanose 12 MSP) and 24g of citrus pectin (ex Hercules) to 3760g of deionised water. The mixture was stirred with a high-speed mixer until the ingredients had dissolved and the dope allowed to stand overnight to degas. It was then spun as in Example 6. It was then tested in the same manner.
Figure 8.1 shows that the alginate/CMC/citrus pectin composite material has the ability to absorb elastase significantly better than that of AQUACEL®, Kaltostat® and Kaltogel®.
Figure 8.2 shows that the amount of elastase activity that was recovered in the washing supernatant was comparable to the comparator dressing materials, in this case two gauze materials (standard surgical gauze and NA™ gauze) and a highly absorbent commercially available surgical dressing (Allevyn™) . When taking into the account the proteinase absorptive capability as shown in Figure 8.1, this composition may also
have a usefulness in modulating proteinase levels in the wound environment.
EXAMPLE 9
A composite fibrous material containing 70% alginate and 30% polyethylene oxide (PEO) was spun from a dope solution containing 6% w/w of solids in water. The dope solution was made by adding 180g of sodium alginate (ex Kalco LF 10/60D) and 60g of PEO (ex Aldrich, 100,000 Dalton MW) to 3760g of deionised water. The mixture was stirred with a high-speed mixer until the ingredients had dissolved and the dope allowed to stand overnight to degas. It was then spun as in Example 6. It was then tested in the same manner.
Figure 9.1 shows that alginate/PEO composite material has the ability to absorb elastase significantly better than that of AQUACEL®, Kaltostat®, Kaltogel® and fibrous gelling pectin.
Figure 9.2 shows that the amount of elastase activity that was recovered in the washing supernatant was comparable to the comparator dressing materials, in this case two gauze materials (standard surgical gauze and NA™ gauze) and a highly absorbent commercially available surgical dressing (Allevyn™) . When taking into the account the proteinase absorptive capability as shown in Figure 9.1, this composition may also have a usefulness in modulating proteinase levels in the wound environment.
EXAMPLE 10
A composite fibrous material containing 50% GENU pectin and 50% citrus pectin was spun from a dope solution containing 10% w/w of solids
in water. The dope solution was made by adding 200g of GENU pectin (ex Hercules) and 200g of citrus pectin (ex Hercules) to 3600g of deionised water. The mixture was stirred with a high-speed mixer until the ingredients had dissolved and the dope allowed to stand overnight to degas. It was then spun as in Example 6. It was then tested in the same manner.
Figure 10.1 shows that the gelling pectin/citrus pectin composite material has the ability to absorb elastase significantly better than that of AQUACEL®, Kaltostat® and Kaltogel®.
Figure 10.2 shows that the amount of elastase activity that was recovered in the washing supernatant was comparable to the comparator dressing material, in this case two gauze materials (standard surgical gauze and NA™ gauze) and a highly absorbent commercially available surgical dressing (Allevyn™) . When taking into the account the proteinase absorptive capability as shown in Figure 10.1 , this composition may also have a usefulness in modulating proteinase levels in the wound environment.
EXAMPLE 11
A composite fibrous material containing 50% GENU pectin and 50% NaCMC was spun from a dope solution containing 10% w/w of solids in water. The dope solution was made by adding 200g GENU pectin (ex Hercules) and 200g of NaCMC (ex Hercules Blanose 12MSP) to 3600g of deionised water. The mixture was stirred with a high-speed mixer until the ingredients had dissolved and the dope allowed to stand overnight to degas. It was then spun as in Example 6. It was then tested in the same manner.
Figure 11.1 shows that the pectin/CMC composite material has the ability to absorb elastase comparable to that of Kaltostat® and Kaltogel®.
Figure 11.2 shows that the amount of elastase activity that was recovered in the washing supernatant was less that that of the comparator dressing materials, it this case two gauze materials (standard surgical gauze and NA™ gauze) and a highly absorbent commercially available surgical dressing (Allevyn™) , indicating better enzyme retention. When taking into the account the proteinase absorptive capability as shown in Figure 11.1, this composition may also have a usefulness in modulating proteinase levels in the wound environment.