WO2002079496A3 - Method and sequences for determinate nucleic acid hybridization - Google Patents

Method and sequences for determinate nucleic acid hybridization Download PDF

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Publication number
WO2002079496A3
WO2002079496A3 PCT/US2002/009928 US0209928W WO02079496A3 WO 2002079496 A3 WO2002079496 A3 WO 2002079496A3 US 0209928 W US0209928 W US 0209928W WO 02079496 A3 WO02079496 A3 WO 02079496A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
hybridization
nucleic acid
methods
interest
Prior art date
Application number
PCT/US2002/009928
Other languages
French (fr)
Other versions
WO2002079496A2 (en
Inventor
William Daniel Hillis
Original Assignee
Applied Minds Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Minds Inc filed Critical Applied Minds Inc
Priority to AT02723698T priority Critical patent/ATE476520T1/en
Priority to EP02723698A priority patent/EP1399588B1/en
Priority to DE60237204T priority patent/DE60237204D1/en
Priority to AU2002254467A priority patent/AU2002254467A1/en
Publication of WO2002079496A2 publication Critical patent/WO2002079496A2/en
Publication of WO2002079496A3 publication Critical patent/WO2002079496A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

Provided are methods for using nucleic acid sequences having two or more degenerately pairing nucleotides, each degenerate nucleotide having a partially overlapping set of complementarity, to reduce the number of hybridizing nucleotide sequences or probes used in biochemical and molecular biological operations having sequence specific hybridization. The method may be employed for various hybridization procedures with sequence specific hybridization, including sequencing methods measuring hybridization directly, and tagging by hybridization methods in which the sequence is determined by analyzing the pattern of tags that hybridize thereto, and hybridization dependent amplification methods. The method involves hybridizing to the nucleic acid sequence of interest a first hybrizing nucleotide sequence and a second hybridizing nucleotide sequence, each comprising a sequence complementary, or complementary except at a position of interest or variable position, to a nucleic acid sequence of interest, and analyzing the whether some, all or none of the probes or tags hybridize.
PCT/US2002/009928 2001-03-28 2002-03-27 Method and sequences for determinate nucleic acid hybridization WO2002079496A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AT02723698T ATE476520T1 (en) 2001-03-28 2002-03-27 METHOD AND SEQUENCES FOR DETERMINATE NUCLEIC ACID HYBRIDIZATION
EP02723698A EP1399588B1 (en) 2001-03-28 2002-03-27 Method and sequences for determinate nucleic acid hybridization
DE60237204T DE60237204D1 (en) 2001-03-28 2002-03-27 METHOD AND SEQUENCES FOR DETERMINING NUCLEIC ACID HYBRIDIZATION
AU2002254467A AU2002254467A1 (en) 2001-03-28 2002-03-27 Method and sequences for determinate nucleic acid hybridization

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/821,694 2001-03-28
US09/821,694 US6949340B2 (en) 2001-03-28 2001-03-28 Optical phase modulator

Publications (2)

Publication Number Publication Date
WO2002079496A2 WO2002079496A2 (en) 2002-10-10
WO2002079496A3 true WO2002079496A3 (en) 2004-01-08

Family

ID=25234067

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/009928 WO2002079496A2 (en) 2001-03-28 2002-03-27 Method and sequences for determinate nucleic acid hybridization

Country Status (6)

Country Link
US (2) US6949340B2 (en)
EP (1) EP1399588B1 (en)
AT (1) ATE476520T1 (en)
AU (1) AU2002254467A1 (en)
DE (1) DE60237204D1 (en)
WO (1) WO2002079496A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949340B2 (en) * 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator
WO2016210224A1 (en) * 2015-06-24 2016-12-29 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease

Citations (4)

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WO1999022025A1 (en) * 1997-10-24 1999-05-06 Trustees Of Boston University Inference sequencing by hybridization
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6461871B1 (en) * 1997-05-09 2002-10-08 Lightup Technologies Ab Method for the preparation of a probe for nucleic acid hybridization

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US5578443A (en) * 1991-03-06 1996-11-26 Regents Of The University Of Minnesota DNA sequence-based HLA typing method
WO1993020096A1 (en) * 1991-05-08 1993-10-14 Stratagene Oligonucleotide libraries useful for producing primers
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US6949340B2 (en) 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6461871B1 (en) * 1997-05-09 2002-10-08 Lightup Technologies Ab Method for the preparation of a probe for nucleic acid hybridization
WO1999022025A1 (en) * 1997-10-24 1999-05-06 Trustees Of Boston University Inference sequencing by hybridization

Also Published As

Publication number Publication date
AU2002254467A1 (en) 2002-10-15
US6949340B2 (en) 2005-09-27
US20070042389A1 (en) 2007-02-22
EP1399588B1 (en) 2010-08-04
US7537892B2 (en) 2009-05-26
US20030134277A1 (en) 2003-07-17
DE60237204D1 (en) 2010-09-16
ATE476520T1 (en) 2010-08-15
EP1399588A4 (en) 2005-09-21
WO2002079496A2 (en) 2002-10-10
EP1399588A2 (en) 2004-03-24
US20040121319A9 (en) 2004-06-24

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