WO2002086159A3 - Method for genotyping microsatellite dna markers - Google Patents

Method for genotyping microsatellite dna markers Download PDF

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Publication number
WO2002086159A3
WO2002086159A3 PCT/IB2002/001287 IB0201287W WO02086159A3 WO 2002086159 A3 WO2002086159 A3 WO 2002086159A3 IB 0201287 W IB0201287 W IB 0201287W WO 02086159 A3 WO02086159 A3 WO 02086159A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna markers
microsatellite dna
genotyping microsatellite
genotyping
nona
Prior art date
Application number
PCT/IB2002/001287
Other languages
French (fr)
Other versions
WO2002086159A2 (en
Inventor
Abdelmajid Belouchi
Bruno Paquin
Original Assignee
Galileo Genomics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Galileo Genomics filed Critical Galileo Genomics
Priority to EP02724508A priority Critical patent/EP1383917A2/en
Priority to CA002445095A priority patent/CA2445095A1/en
Publication of WO2002086159A2 publication Critical patent/WO2002086159A2/en
Publication of WO2002086159A3 publication Critical patent/WO2002086159A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

The present invention provides a high throughput method for genotyping microsatellite markers. The technology uses a combination of oligonucleotides in a selective ligation reaction that discriminates mono-, di-, tri,- tetra-, penta-, hexa-, hepta-, octa- and nona-nucleotide repeated alleles in amplified DNA from individuals.
PCT/IB2002/001287 2001-04-23 2002-04-19 Method for genotyping microsatellite dna markers WO2002086159A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02724508A EP1383917A2 (en) 2001-04-23 2002-04-19 Method for genotyping microsatellite dna markers
CA002445095A CA2445095A1 (en) 2001-04-23 2002-04-19 Method for genotyping microsatellite dna markers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/840,717 US6479244B1 (en) 2001-04-23 2001-04-23 Method for genotyping microsatellite DNA markers
US09/840,717 2001-04-23

Publications (2)

Publication Number Publication Date
WO2002086159A2 WO2002086159A2 (en) 2002-10-31
WO2002086159A3 true WO2002086159A3 (en) 2003-10-23

Family

ID=25283033

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/001287 WO2002086159A2 (en) 2001-04-23 2002-04-19 Method for genotyping microsatellite dna markers

Country Status (4)

Country Link
US (1) US6479244B1 (en)
EP (1) EP1383917A2 (en)
CA (1) CA2445095A1 (en)
WO (1) WO2002086159A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
CA2510587A1 (en) 2002-12-20 2004-07-15 Qiagen Gmbh Nucleic acid amplification
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
EP1762627A1 (en) 2005-09-09 2007-03-14 Qiagen GmbH Method for the activation of a nucleic acid for performing a polymerase reaction
DE102007010311A1 (en) * 2007-02-23 2008-08-28 Thines, Marco, Dr. Organism-specific hybridizable nucleic acid molecule
RU2414510C2 (en) * 2007-06-14 2011-03-20 Институт биохимии и генетики Уфимского научного центра РАН Method of amplifying specific nucleic acid fragments through recurrent chain reaction
KR102252447B1 (en) * 2011-05-04 2021-05-17 바이오셉트 인코포레이티드 Methods for detecting nucleic acid sequence variants
KR101356396B1 (en) 2011-11-30 2014-02-05 한국과학기술원 PCR-free Minisatellite Tandem Repeat Genotyping
DE102013221402A1 (en) * 2013-10-22 2015-04-23 Siemens Aktiengesellschaft Method for detecting and quantifying a single-stranded target nucleic acid
WO2017051439A1 (en) * 2015-09-21 2017-03-30 UNIVERSITA' DEGLI STUDI Dl MESSINA Haplotypes of d7s 6440 microsatellite internal to the hipk2 gene as markers of autoimmune thyroiditis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025139A (en) * 1995-08-30 2000-02-15 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6303301B1 (en) * 1997-01-13 2001-10-16 Affymetrix, Inc. Expression monitoring for gene function identification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025139A (en) * 1995-08-30 2000-02-15 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LUO J ET AL: "Improving the fidelity of Thermus thermophilus DNA ligase", NUCLEIC ACIDS RESEARCH, IRL PRESS LTD., OXFORD, GB, vol. 24, no. 14, 1 August 1996 (1996-08-01), pages 3071 - 3078, XP002141352, ISSN: 0305-1048 *
ZANDER CECILIA ET AL: "Multivariate analysis of factors influencing repeat expansion detection.", GENOME RESEARCH, vol. 8, no. 10, October 1998 (1998-10-01), pages 1085 - 1094, XP002246191, ISSN: 1088-9051 *
ZIRVI M ET AL: "Improved fidelity of thermostable ligases for detection of microsatellite repeat sequences using nucleoside analogs.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 DEC 1999, vol. 27, no. 24, 15 December 1999 (1999-12-15), pages e41, XP002246189, ISSN: 1362-4962 *
ZIRVI M ET AL: "Ligase-based detection of mononucleotide repeat sequences.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 DEC 1999, vol. 27, no. 24, 15 December 1999 (1999-12-15), pages e40, XP002246190, ISSN: 1362-4962 *

Also Published As

Publication number Publication date
EP1383917A2 (en) 2004-01-28
WO2002086159A2 (en) 2002-10-31
US20020155449A1 (en) 2002-10-24
US6479244B1 (en) 2002-11-12
CA2445095A1 (en) 2002-10-31

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