WO2002092845A3 - Method for determining enzymatic activity of glycosyl transferase - Google Patents

Method for determining enzymatic activity of glycosyl transferase Download PDF

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Publication number
WO2002092845A3
WO2002092845A3 PCT/EP2002/005101 EP0205101W WO02092845A3 WO 2002092845 A3 WO2002092845 A3 WO 2002092845A3 EP 0205101 W EP0205101 W EP 0205101W WO 02092845 A3 WO02092845 A3 WO 02092845A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymatic activity
glycosyl transferase
glycosyl
transferase
determining enzymatic
Prior art date
Application number
PCT/EP2002/005101
Other languages
German (de)
French (fr)
Other versions
WO2002092845A2 (en
Inventor
Gerd Hummel
Laurence Jobron
Keiko Sujino
Monica M Palcic
Original Assignee
Jerini Ag
Gerd Hummel
Laurence Jobron
Keiko Sujino
Monica M Palcic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jerini Ag, Gerd Hummel, Laurence Jobron, Keiko Sujino, Monica M Palcic filed Critical Jerini Ag
Priority to AU2002342841A priority Critical patent/AU2002342841A1/en
Publication of WO2002092845A2 publication Critical patent/WO2002092845A2/en
Publication of WO2002092845A3 publication Critical patent/WO2002092845A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

The invention relates to a method for determining the enzymatic activity of glycosyl transferase in a sample, wherein the sample is reacted with a glycosyl donor and a glycosyl receptor immobilized on a carrier, especially a cellulose membrane, wherein a transferase product is determined in the presence of a enzymatic glycosyl transferase activity and the carrier is a cellulose membrane.
PCT/EP2002/005101 2001-05-09 2002-05-08 Method for determining enzymatic activity of glycosyl transferase WO2002092845A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002342841A AU2002342841A1 (en) 2001-05-09 2002-05-08 Method for determining enzymatic activity of glycosyl transferase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10122452.4 2001-05-09
DE2001122452 DE10122452A1 (en) 2001-05-09 2001-05-09 Method for the detection of the enzymatic activity of glycosyltransferases

Publications (2)

Publication Number Publication Date
WO2002092845A2 WO2002092845A2 (en) 2002-11-21
WO2002092845A3 true WO2002092845A3 (en) 2004-03-11

Family

ID=7684111

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/005101 WO2002092845A2 (en) 2001-05-09 2002-05-08 Method for determining enzymatic activity of glycosyl transferase

Country Status (3)

Country Link
AU (1) AU2002342841A1 (en)
DE (1) DE10122452A1 (en)
WO (1) WO2002092845A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0421368D0 (en) * 2004-09-25 2004-10-27 Univ York Glycosyltransferase assay
US9410182B2 (en) 2010-05-24 2016-08-09 Bio-Techne Corporation Phosphatase coupled glycosyltransferase assay

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0387875A2 (en) * 1989-03-15 1990-09-19 Otsuka Pharmaceutical Company Limited Method for determination of activities of glycosyltransferases and its use
US5173407A (en) * 1986-12-16 1992-12-22 Konica Corporation Method for measuring glycosyltransferase
WO1994010340A1 (en) * 1992-10-30 1994-05-11 Mt. Sinai Hospital Corporation Method for measuring glycosyltransferase activity

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173407A (en) * 1986-12-16 1992-12-22 Konica Corporation Method for measuring glycosyltransferase
EP0387875A2 (en) * 1989-03-15 1990-09-19 Otsuka Pharmaceutical Company Limited Method for determination of activities of glycosyltransferases and its use
WO1994010340A1 (en) * 1992-10-30 1994-05-11 Mt. Sinai Hospital Corporation Method for measuring glycosyltransferase activity

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BAXTER A ET AL: "A RAPID SENSITIVE DISC ASSAY FOR THE DETERMINATION OF GLYCO PROTEIN GLYCOSYL TRANSFERASES", ANALYTICAL BIOCHEMISTRY, vol. 98, no. 1, 1979, pages 95 - 101, XP009011760, ISSN: 0003-2697 *
GU, XINBIN ET AL: "Direct Assay of Glycosphingolipid Glycosyltransferase Activities on Thin-Layer Chromatogram", ANALTICAL BIOCHEMISTRY, vol. 185, 1990, pages 151 - 155, XP009017591 *
REINEKE U ET AL: "Applications of peptide arrays prepared by the SPOT-technology", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 12, no. 1, February 2001 (2001-02-01), pages 59 - 64, XP002234261, ISSN: 0958-1669 *
SADLER J E ET AL: "PURIFICATION OF MAMMALIAN GLYCOSYLTRANSFERASES", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 83, 1982, pages 458 - 514, XP000749292, ISSN: 0076-6879 *
WENSCHUH HOLGER ET AL: "Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides.", BIOPOLYMERS, vol. 55, no. 3, 2000, pages 188 - 206, XP002255029, ISSN: 0006-3525 *

Also Published As

Publication number Publication date
WO2002092845A2 (en) 2002-11-21
AU2002342841A1 (en) 2002-11-25
DE10122452A1 (en) 2003-02-27

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