WO2003008931A3 - Microstructure for particle and cell separation, identification, sorting, and manipulation - Google Patents

Microstructure for particle and cell separation, identification, sorting, and manipulation Download PDF

Info

Publication number
WO2003008931A3
WO2003008931A3 PCT/US2002/022689 US0222689W WO03008931A3 WO 2003008931 A3 WO2003008931 A3 WO 2003008931A3 US 0222689 W US0222689 W US 0222689W WO 03008931 A3 WO03008931 A3 WO 03008931A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
void
microstructure
sorting
manipulation
Prior art date
Application number
PCT/US2002/022689
Other languages
French (fr)
Other versions
WO2003008931A2 (en
Inventor
Georgi Hvichia
Paolo Gasparini
Original Assignee
Georgi Hvichia
Paolo Gasparini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Georgi Hvichia, Paolo Gasparini filed Critical Georgi Hvichia
Priority to US10/587,053 priority Critical patent/US7993908B2/en
Priority to AU2002320576A priority patent/AU2002320576A1/en
Publication of WO2003008931A2 publication Critical patent/WO2003008931A2/en
Publication of WO2003008931A3 publication Critical patent/WO2003008931A3/en
Priority to US13/170,369 priority patent/US8765456B2/en
Priority to US14/079,333 priority patent/US9534200B2/en
Priority to US14/079,319 priority patent/US9534199B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/30—Filter housing constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0272—Investigating particle size or size distribution with screening; with classification by filtering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N2015/0288—Sorting the particles

Abstract

The invention relates to microscale cell separating apparatus which are able to separate cells on the basis of size of the cells, interaction of the cells with surfaces of the apparatus, or both. The apparatus comprises a stepped or sloped separation element (16) interposed between an inlet region (20) and an outlet region (22) of a void that can be filled with fluid. The void can be enclosed within a cover (12) and fluid flow through the void engages cells with the separation element. Only cells which have (or can deform to have) a characteristic dimension smaller than or equal to the distance between a step and the cover or body can pass onto or past a step. Modifications of surfaces within the apparatus can also inhibit passage of cells onto or past a step.
PCT/US2002/022689 2001-07-17 2002-07-17 Microstructure for particle and cell separation, identification, sorting, and manipulation WO2003008931A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/587,053 US7993908B2 (en) 2001-07-17 2002-07-17 Microstructure for particle and cell separation, identification, sorting, and manipulation
AU2002320576A AU2002320576A1 (en) 2001-07-17 2002-07-17 Microstructure for particle and cell separation, identification, sorting, and manipulation
US13/170,369 US8765456B2 (en) 2001-07-17 2011-06-28 Microstructure for particle and cell separation, identification, sorting, and manipulation
US14/079,333 US9534200B2 (en) 2001-07-17 2013-11-13 Microstructure for particle and cell SEparation, identification, sorting, and manipulation
US14/079,319 US9534199B2 (en) 2001-07-17 2013-11-13 Microstructure for particle and cell separation, identification, sorting, and manipulation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30629601P 2001-07-17 2001-07-17
US60/306,296 2001-07-17

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10/587,053 A-371-Of-International US7993908B2 (en) 2001-07-17 2002-07-17 Microstructure for particle and cell separation, identification, sorting, and manipulation
US13/170,369 Continuation US8765456B2 (en) 2001-07-17 2011-06-28 Microstructure for particle and cell separation, identification, sorting, and manipulation
US13/170,309 Continuation US20120006172A1 (en) 2010-07-10 2011-06-28 Device for separating packages from a carrier belt having successively arranged blister bubbles

Publications (2)

Publication Number Publication Date
WO2003008931A2 WO2003008931A2 (en) 2003-01-30
WO2003008931A3 true WO2003008931A3 (en) 2003-11-06

Family

ID=23184670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/022689 WO2003008931A2 (en) 2001-07-17 2002-07-17 Microstructure for particle and cell separation, identification, sorting, and manipulation

Country Status (3)

Country Link
US (4) US7993908B2 (en)
AU (1) AU2002320576A1 (en)
WO (1) WO2003008931A2 (en)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002320576A1 (en) 2001-07-17 2003-03-03 Paolo Gasparini Microstructure for particle and cell separation, identification, sorting, and manipulation
US7514045B2 (en) 2002-01-18 2009-04-07 Avery Dennison Corporation Covered microchamber structures
ATE531257T1 (en) 2002-09-27 2011-11-15 Gen Hospital Corp MICROFLUIDIC DEVICE FOR CELL SEPARATION AND USES THEREOF
US20070039463A1 (en) * 2003-10-02 2007-02-22 Gert Desmet Method and device for size-separating particles present in a fluid
JP2006058195A (en) * 2004-08-23 2006-03-02 Alps Electric Co Ltd Inspection plate and inspection method using same
JP4185904B2 (en) * 2004-10-27 2008-11-26 株式会社日立ハイテクノロジーズ Liquid transfer substrate, analysis system, and analysis method
US20070026418A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
EP2477029A1 (en) * 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20090181421A1 (en) * 2005-07-29 2009-07-16 Ravi Kapur Diagnosis of fetal abnormalities using nucleated red blood cells
US20070059781A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for size based separation and analysis
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
EP2061801A4 (en) * 2006-06-14 2009-11-11 Living Microsystems Inc Diagnosis of fetal abnormalities by comparative genomic hybridization analysis
US20080070792A1 (en) * 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
JP2008082896A (en) * 2006-09-27 2008-04-10 Fujifilm Corp Blood plasma recovery method and tool
EP2427568A4 (en) * 2008-04-23 2015-06-10 Parsortix Inc Methods and apparatus for segregation of particles
AU2010246381B2 (en) * 2008-04-23 2013-08-15 Angle North America, Inc. Methods and apparatus for segregation of particles
CA3069077C (en) 2008-09-20 2022-03-22 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8192685B2 (en) 2008-11-04 2012-06-05 Advanced Concepts And Technologies International, L.L.C. Molecular separators, concentrators, and detectors preparatory to sensor operation, and methods of minimizing false positives in sensor operations
WO2010085815A1 (en) * 2009-01-26 2010-07-29 Artemis Health, Inc. Methods and compositions for identifying a fetal cell
NZ595538A (en) * 2009-03-10 2014-04-30 Univ Monash Platelet aggregation using a microfluidics device
KR101257700B1 (en) * 2011-12-05 2013-04-24 삼성전자주식회사 Microfluidic device and microfluidic system including thereof
CN103160430A (en) * 2011-12-13 2013-06-19 三星电子株式会社 Cell capturing filter having high aspect ratio
WO2013177206A2 (en) 2012-05-21 2013-11-28 Fluidigm Corporation Single-particle analysis of particle populations
WO2014093444A1 (en) * 2012-12-11 2014-06-19 Atmi Packaging, Inc. Recipient for cell cultivation
US9631179B2 (en) 2013-03-15 2017-04-25 Angle North America, Inc. Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge
US10324011B2 (en) 2013-03-15 2019-06-18 The Trustees Of Princeton University Methods and devices for high throughput purification
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
CN110186835B (en) 2013-03-15 2022-05-31 Gpb科学有限公司 On-chip microfluidic processing of particles
US20150290368A1 (en) * 2014-04-09 2015-10-15 Parsortix, Inc. Removal of Particulates from Blood Using an Apparatus Including a Size-Differentiating Element
KR102528348B1 (en) * 2015-03-16 2023-05-03 루미넥스 코포레이션 Device and method for multi-stepped channel emulsification
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
US10207266B2 (en) * 2015-09-29 2019-02-19 Foxconn Interconnect Technology Limited Microfluidic device for detecting cells of blood
WO2017093780A1 (en) * 2015-11-30 2017-06-08 Rqmicro Ag Microfluidic device, assemblies, and method for extracting particles from a sample
JP7387430B2 (en) 2016-06-27 2023-11-28 ゾエティス サービシズ リミテッド ライアビリティ カンパニー Devices with modified conduits
US11268892B2 (en) 2016-11-09 2022-03-08 Georgia Tech Research Corporation Methods for continuous sorting of cells based on molecular adhesion
FR3061719B1 (en) * 2017-01-06 2021-02-12 Commissariat Energie Atomique MICROFLUIDIC SYSTEM FOR HANDLING BIOLOGICAL CELLS
JP2020506699A (en) 2017-01-31 2020-03-05 ジェネア アイピー ホールディングス ピーティーワイ リミテッド Methods and systems for processing biological samples
EP3612307A4 (en) 2017-04-21 2021-06-09 Zoetis Services LLC Systems, devices and methods for microfluidic analysis
CN107729932B (en) * 2017-10-10 2019-07-26 杭州智微信息科技有限公司 Bone marrow cell labeling method and system
US11524293B2 (en) 2018-01-17 2022-12-13 Sartorius Stedim North America Inc. Cell separation device, method and system
US11389799B2 (en) * 2019-01-17 2022-07-19 The Regents Of The University Of Michigan Microfluidic device for size and deformability measurements and applications thereof
US20220146400A1 (en) * 2019-02-27 2022-05-12 Kyocera Corporation Particle separating and measuring device and particle separating and measuring apparatus
CN112831394A (en) * 2019-11-25 2021-05-25 香港城市大学深圳研究院 Micro-fluidic chip
EP4232554A1 (en) 2020-11-18 2023-08-30 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
US5866345A (en) * 1992-05-01 1999-02-02 The Trustees Of The University Of Pennsylvania Apparatus for the detection of an analyte utilizing mesoscale flow systems
US6004821A (en) * 1998-03-07 1999-12-21 Levine; Robert A. Method and apparatus for performing chemical, qualitative, quantitative, and semi-quantitative analyses of a urine sample
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5089384A (en) * 1988-11-04 1992-02-18 Amoco Corporation Method and apparatus for selective cell destruction using amplified immunofluorescence
JP2685544B2 (en) * 1988-11-11 1997-12-03 株式会社日立製作所 Blood filter, blood test method, and blood test apparatus
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5427663A (en) * 1993-06-08 1995-06-27 British Technology Group Usa Inc. Microlithographic array for macromolecule and cell fractionation
FR2782730B1 (en) 1998-08-25 2002-05-17 Biocom Sa CELL SEPARATION PROCESS FOR THE ISOLATION OF PATHOGENIC CELLS, PARTICULARLY RARE CANCERES, EQUIPMENT AND REAGENT FOR IMPLEMENTING THE PROCESS AND APPLICATION OF THE PROCESS
FR2824144B1 (en) 2001-04-30 2004-09-17 Metagenex S A R L METHOD OF PRENATAL DIAGNOSIS ON FETAL CELLS ISOLATED FROM MATERNAL BLOOD
US6783928B2 (en) * 2001-07-17 2004-08-31 Georgi Hvichia Microstructures for cell proliferation assays and semen analysis
AU2002320576A1 (en) 2001-07-17 2003-03-03 Paolo Gasparini Microstructure for particle and cell separation, identification, sorting, and manipulation
ATE531257T1 (en) * 2002-09-27 2011-11-15 Gen Hospital Corp MICROFLUIDIC DEVICE FOR CELL SEPARATION AND USES THEREOF
WO2006078470A2 (en) 2005-01-18 2006-07-27 Biocept, Inc. Cell separation using microchannel having patterned posts

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866345A (en) * 1992-05-01 1999-02-02 The Trustees Of The University Of Pennsylvania Apparatus for the detection of an analyte utilizing mesoscale flow systems
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins
US6004821A (en) * 1998-03-07 1999-12-21 Levine; Robert A. Method and apparatus for performing chemical, qualitative, quantitative, and semi-quantitative analyses of a urine sample

Also Published As

Publication number Publication date
US20120052567A1 (en) 2012-03-01
WO2003008931A2 (en) 2003-01-30
US20070072290A1 (en) 2007-03-29
US20140072953A1 (en) 2014-03-13
AU2002320576A1 (en) 2003-03-03
US20140072952A1 (en) 2014-03-13
US20160340644A9 (en) 2016-11-24
US9534199B2 (en) 2017-01-03
US8765456B2 (en) 2014-07-01
US7993908B2 (en) 2011-08-09
US20160376553A9 (en) 2016-12-29
US9534200B2 (en) 2017-01-03

Similar Documents

Publication Publication Date Title
WO2003008931A3 (en) Microstructure for particle and cell separation, identification, sorting, and manipulation
MXPA05001654A (en) High resolution flow cytometer.
AU7964701A (en) Particle trap for separating particles from the flow of a liquid, method for separating particles from the flow of liquid and use of said particle trap
EP2664666A3 (en) Devices and methods for enrichment and alteration of cells and other particles
WO2004059474A3 (en) Micromachined intergrated fluid delivery system
TW343141B (en) Apparatus for separating particles from a fluid flow and a valve for introducing bled fluid to a mainstream fluid
EP1584922A3 (en) Particle packing of microdevice
WO2005065126A3 (en) Blood filter assembly having multiple filtration regions
WO2001095780A3 (en) Method and apparatus of particle transfer in multi-stage particle separators
WO2008130977A3 (en) Systems and methods for particle focusing in microchannels
WO2003056330A3 (en) Cell sorting system for the size-based sorting or separation of cells suspended in a flowing fluid
WO2000074815A3 (en) Device for separating a mixture of gas with liuqid and/or solid material
WO2005023391A3 (en) Multiple laminar flow-based particle and cellular separation with laser steering
EP1512362A3 (en) Apparatus and method for separating particles from a cyclonic fluid flow
WO2008077954A3 (en) Fuel filter
WO2002086425A3 (en) Airflow measuring device with a device for separating foreign particles
HK1071528A1 (en) Valve element
SE0300145D0 (en) Cyclone device for separating particles from a fluid stream
ES8304812A1 (en) Improvements in and relating to cyclone separators.
ATE266478T1 (en) CYCLONE SEPARATOR FOR VARIABLE FLOW RATES
WO2003007711A8 (en) Microstructures for cell proliferation assays and semen analysis
CA2394935A1 (en) Installation for measuring concentration in dense granular material of a flow and water treatment system comprising same
SE9400999D0 (en) Vessels for mixing or separating streaming media
AU2002225568A1 (en) Filter for cooling water in a light water cooled nuclear reactor
AU4801899A (en) Pollen grain counting method and pollen grain counter

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: JP

WWE Wipo information: entry into national phase

Ref document number: 2007072290

Country of ref document: US

Ref document number: 10587053

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 10587053

Country of ref document: US