WO2003010506A3 - Combined electronic-and photonic-based hybridation assay - Google Patents

Combined electronic-and photonic-based hybridation assay Download PDF

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Publication number
WO2003010506A3
WO2003010506A3 PCT/IB2002/002788 IB0202788W WO03010506A3 WO 2003010506 A3 WO2003010506 A3 WO 2003010506A3 IB 0202788 W IB0202788 W IB 0202788W WO 03010506 A3 WO03010506 A3 WO 03010506A3
Authority
WO
WIPO (PCT)
Prior art keywords
signals
assay
hybridation
photonic
combined electronic
Prior art date
Application number
PCT/IB2002/002788
Other languages
French (fr)
Other versions
WO2003010506A2 (en
Inventor
Glen H Erikson
Jasmine I Daksis
Pierre Picard
Original Assignee
Ingeneus Corp
Glen H Erikson
Jasmine I Daksis
Pierre Picard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp, Glen H Erikson, Jasmine I Daksis, Pierre Picard filed Critical Ingeneus Corp
Priority to BR0211350-3A priority Critical patent/BR0211350A/en
Priority to NZ531259A priority patent/NZ531259A/en
Priority to MXPA04000735A priority patent/MXPA04000735A/en
Priority to EP02745718A priority patent/EP1461452A4/en
Priority to AU2002317427A priority patent/AU2002317427B2/en
Priority to KR1020047000960A priority patent/KR100617427B1/en
Priority to IL15992802A priority patent/IL159928A0/en
Priority to JP2003515830A priority patent/JP2005527779A/en
Priority to CA002454415A priority patent/CA2454415A1/en
Publication of WO2003010506A2 publication Critical patent/WO2003010506A2/en
Publication of WO2003010506A3 publication Critical patent/WO2003010506A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/307—Denaturation or renaturation by electric current/voltage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels

Abstract

A method for homogeneously assaying biopolymer bonding includes obtaining signals from a test sample before, during and/or after the application of stimulus to the test sample and correlating the signals. The signals, whose magnitude correlate with binding affinity, can be, for example, electrical conductance and/or fluorescent intensity. The stimulus can be, for example, electric voltage and/or laser radiation. Preferably, different types of signals are measured and compared so as to enhance the reliability of the assay.
PCT/IB2002/002788 2001-07-23 2002-07-15 Combined electronic-and photonic-based hybridation assay WO2003010506A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BR0211350-3A BR0211350A (en) 2001-07-23 2002-07-15 Homogeneous biopolymer binding assay by multiple measurements under varying conditions
NZ531259A NZ531259A (en) 2001-07-23 2002-07-15 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
MXPA04000735A MXPA04000735A (en) 2001-07-23 2002-07-15 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions.
EP02745718A EP1461452A4 (en) 2001-07-23 2002-07-15 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
AU2002317427A AU2002317427B2 (en) 2001-07-23 2002-07-15 Combined electronic-and photonic-based hybridation assay
KR1020047000960A KR100617427B1 (en) 2001-07-23 2002-07-15 Homogeneous Assay of Biopolymer Binding by Means of Multiple Measurements Under Varied Conditions
IL15992802A IL159928A0 (en) 2001-07-23 2002-07-15 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
JP2003515830A JP2005527779A (en) 2001-07-23 2002-07-15 Homogeneous assay of biopolymer binding by multiple measurements under different conditions
CA002454415A CA2454415A1 (en) 2001-07-23 2002-07-15 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/911,047 2001-07-23
US09/911,047 US7220541B2 (en) 2000-01-24 2001-07-23 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions

Publications (2)

Publication Number Publication Date
WO2003010506A2 WO2003010506A2 (en) 2003-02-06
WO2003010506A3 true WO2003010506A3 (en) 2004-07-22

Family

ID=25429687

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/002788 WO2003010506A2 (en) 2001-07-23 2002-07-15 Combined electronic-and photonic-based hybridation assay

Country Status (16)

Country Link
US (1) US7220541B2 (en)
EP (1) EP1461452A4 (en)
JP (1) JP2005527779A (en)
KR (1) KR100617427B1 (en)
CN (1) CN1671862A (en)
AU (1) AU2002317427B2 (en)
BR (1) BR0211350A (en)
CA (1) CA2454415A1 (en)
CO (1) CO5560630A2 (en)
IL (1) IL159928A0 (en)
MX (1) MXPA04000735A (en)
NZ (1) NZ531259A (en)
RU (1) RU2295572C2 (en)
TW (1) TWI274875B (en)
WO (1) WO2003010506A2 (en)
ZA (1) ZA200401363B (en)

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US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
DE10251757B4 (en) * 2002-11-05 2006-03-09 Micronas Holding Gmbh Device for determining the concentration of ligands contained in a sample to be examined
US20060094016A1 (en) * 2002-12-02 2006-05-04 Niall Gormley Determination of methylation of nucleic acid sequences
US20040142329A1 (en) * 2003-01-17 2004-07-22 Ingeneus Corporation Probe conjugation to increase multiplex binding motif preference
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
CA2581087C (en) * 2004-09-24 2013-11-19 Ingeneus Inc. Genomic assay
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US8541207B2 (en) 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
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Also Published As

Publication number Publication date
CN1671862A (en) 2005-09-21
BR0211350A (en) 2005-05-17
KR20040024588A (en) 2004-03-20
TWI274875B (en) 2007-03-01
AU2002317427B2 (en) 2006-08-17
US7220541B2 (en) 2007-05-22
EP1461452A4 (en) 2006-06-21
EP1461452A2 (en) 2004-09-29
NZ531259A (en) 2008-04-30
JP2005527779A (en) 2005-09-15
MXPA04000735A (en) 2004-07-08
CO5560630A2 (en) 2005-09-30
US20020137056A1 (en) 2002-09-26
KR100617427B1 (en) 2006-08-30
RU2004105151A (en) 2005-05-27
IL159928A0 (en) 2004-06-20
RU2295572C2 (en) 2007-03-20
ZA200401363B (en) 2004-08-27
CA2454415A1 (en) 2003-02-06
WO2003010506A2 (en) 2003-02-06

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