WO2003044037A3 - Process for the manufacture of human mononuclear phagocytic leukocytes - Google Patents

Process for the manufacture of human mononuclear phagocytic leukocytes Download PDF

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Publication number
WO2003044037A3
WO2003044037A3 PCT/IL2002/000930 IL0200930W WO03044037A3 WO 2003044037 A3 WO2003044037 A3 WO 2003044037A3 IL 0200930 W IL0200930 W IL 0200930W WO 03044037 A3 WO03044037 A3 WO 03044037A3
Authority
WO
WIPO (PCT)
Prior art keywords
phagocytic leukocytes
manufacture
mononuclear phagocytic
human mononuclear
human
Prior art date
Application number
PCT/IL2002/000930
Other languages
French (fr)
Other versions
WO2003044037A2 (en
Inventor
Valentin Fulga
Bakimer Ronit Kleiner
Issar Ash
Michal Eisenbach-Schwartz
Original Assignee
Yeda Res & Dev
Valentin Fulga
Bakimer Ronit Kleiner
Issar Ash
Michal Eisenbach-Schwartz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to MXPA04004813A priority Critical patent/MXPA04004813A/en
Priority to NZ533033A priority patent/NZ533033A/en
Priority to EP02788490A priority patent/EP1456355A4/en
Priority to IL16209402A priority patent/IL162094A0/en
Priority to CA2467046A priority patent/CA2467046C/en
Priority to US10/496,352 priority patent/US8377692B2/en
Application filed by Yeda Res & Dev, Valentin Fulga, Bakimer Ronit Kleiner, Issar Ash, Michal Eisenbach-Schwartz filed Critical Yeda Res & Dev
Priority to AU2002353465A priority patent/AU2002353465B2/en
Priority to KR1020047007695A priority patent/KR101001119B1/en
Priority to JP2003545673A priority patent/JP2005519585A/en
Publication of WO2003044037A2 publication Critical patent/WO2003044037A2/en
Publication of WO2003044037A3 publication Critical patent/WO2003044037A3/en
Priority to IL162094A priority patent/IL162094A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4614—Monocytes; Macrophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
    • A61K39/4622—Antigen presenting cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterised by the dose, timing or administration schedule
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts

Abstract

A process for the manufacture of human mononuclear phagocytic leukocytes comprises incubating monocytes isolated from a blood sample of an individual and skin segments from the same individual, removing the dermis segments from the incubation mixture and sedimenting the obtained activated mononuclear phagocytic leukocytes by centrifugation, washing and resuspending the activated phagocytic leukocytes in the medium, and evaluating the culture for its suitability for human administration. Cellular therapy products are made from the obtained cultures and are useful for promoting axonal regeneration in the CNS, wound healing and treatment of myocardial infarction.
PCT/IL2002/000930 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes WO2003044037A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
NZ533033A NZ533033A (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes
EP02788490A EP1456355A4 (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes
IL16209402A IL162094A0 (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes
CA2467046A CA2467046C (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes
US10/496,352 US8377692B2 (en) 2001-11-21 2002-11-21 Process for the manufacturing of human mononuclear phagocytic leukocytes
MXPA04004813A MXPA04004813A (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes.
AU2002353465A AU2002353465B2 (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes
KR1020047007695A KR101001119B1 (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes
JP2003545673A JP2005519585A (en) 2001-11-21 2002-11-21 Method for producing human mononuclear phagocytic leukocytes
IL162094A IL162094A (en) 2001-11-21 2004-05-20 Process for the manufacture of a culture of activated human mononuclear phagocytic leukocytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33178001P 2001-11-21 2001-11-21
US60/331,780 2001-11-21

Publications (2)

Publication Number Publication Date
WO2003044037A2 WO2003044037A2 (en) 2003-05-30
WO2003044037A3 true WO2003044037A3 (en) 2004-04-08

Family

ID=23295342

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2002/000930 WO2003044037A2 (en) 2001-11-21 2002-11-21 Process for the manufacture of human mononuclear phagocytic leukocytes

Country Status (11)

Country Link
US (1) US8377692B2 (en)
EP (1) EP1456355A4 (en)
JP (2) JP2005519585A (en)
KR (2) KR101001119B1 (en)
CN (1) CN1615357A (en)
AU (1) AU2002353465B2 (en)
CA (1) CA2467046C (en)
IL (2) IL162094A0 (en)
MX (1) MXPA04004813A (en)
NZ (1) NZ533033A (en)
WO (1) WO2003044037A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060210543A1 (en) * 2005-03-21 2006-09-21 Jonathan Leor Method of treating infarcted myocardium
US9220813B2 (en) * 2005-04-18 2015-12-29 Holy Cross Hospital, Inc. Cell therapy for limiting overzealous inflammatory reactions in tissue healing
PL1951864T3 (en) * 2005-11-07 2014-10-31 Pecora & Co Llc Compositions and methods of vascular injury repair
US20110076255A1 (en) 2005-11-07 2011-03-31 Pecora Andrew L Compositions and methods for treating progressive myocardial injury due to a vascular insufficiency
US8637005B2 (en) 2005-11-07 2014-01-28 Amorcyte, Inc. Compositions and methods of vascular injury repair
RU2535966C2 (en) * 2008-09-12 2014-12-20 Криопрашис Криобиоложия Лтда. Cell-based therapy of ischemic tissue
WO2010065601A1 (en) * 2008-12-03 2010-06-10 Amorcyte, Inc. Infarct area perfusion-improving compositions and methods of vascular injury repair
US8574902B2 (en) 2009-03-05 2013-11-05 Macrocure Ltd. Activated leukocyte composition and uses for wound healing
CA2754190A1 (en) * 2009-03-05 2010-09-10 Macrocure, Ltd. Activated leukocyte composition
JP2013537889A (en) 2010-09-09 2013-10-07 マクロキュア，リミテッド Activated leukocyte conditioned supernatant and use for wound healing
KR20140107439A (en) * 2011-12-14 2014-09-04 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 Human monocyte sub-population for treatment of central nervous system injury
EP2662085A1 (en) * 2012-05-11 2013-11-13 Humboldt-Universität zu Berlin Regulatory Macrophages and Their Uses
CN102703597B (en) * 2012-06-20 2013-10-30 安徽信灵检验医学科技有限公司 Blood leucocyte separating medium and preparation method and vacuum blood collection tube
AU2014269935B2 (en) 2013-05-22 2019-01-24 Yeda Research And Development Co. Ltd Human monocyte sub-population for treatment of eye diseases and disorders

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800812A (en) * 1995-09-15 1998-09-01 Yeda Research And Development Co. Ltd. Methods of use of mononuclear phagocytes to promote axonal regeneration
US6043089A (en) * 1995-03-07 2000-03-28 Menicon Co., Ltd. Skin culture and process for preparing the same

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US4086057A (en) * 1976-07-28 1978-04-25 William Clinton Everett Ultrasonic disinfection system
US5292655A (en) * 1990-01-29 1994-03-08 Wille Jr John J Method for the formation of a histologically-complete skin substitute
US6267955B1 (en) 1995-09-15 2001-07-31 Yeda Research And Development Co. Ltd. Mononuclear phagocytes and their use to promote axonal regeneration
US6060515A (en) * 1997-01-24 2000-05-09 The Regents Of The University Of California Treatment of skin conditions by use of PPARα activators
WO1998037881A1 (en) * 1997-02-28 1998-09-03 Warner Lambert Company Method of treating or preventing septic shock by administering a mek inhibitor
CA2320491A1 (en) 1998-03-30 1999-10-07 I.D.M. Immuno-Designed Molecules Stimulated monocyte derived cells, their preparation and uses
US6893462B2 (en) * 2000-01-11 2005-05-17 Regeneration Technologies, Inc. Soft and calcified tissue implants
US20030109583A1 (en) * 2001-07-25 2003-06-12 Raju Bore G. Administration of negamycin or deoxynegamycin for the treatment of bacterial infections

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6043089A (en) * 1995-03-07 2000-03-28 Menicon Co., Ltd. Skin culture and process for preparing the same
US5800812A (en) * 1995-09-15 1998-09-01 Yeda Research And Development Co. Ltd. Methods of use of mononuclear phagocytes to promote axonal regeneration

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SEPULVEDA-MERRIIL ET AL.: "Antigen-presenting capacity in normal human dermis is mainly subserved by CD1a+ cells", BRITISH JOURNAL OF DERMATOLOGY, vol. 131, 1994, pages 15 - 22, XP001068867 *

Also Published As

Publication number Publication date
MXPA04004813A (en) 2004-08-11
KR20100087781A (en) 2010-08-05
JP2010100653A (en) 2010-05-06
IL162094A0 (en) 2005-11-20
WO2003044037A2 (en) 2003-05-30
JP5087094B2 (en) 2012-11-28
US20050129663A1 (en) 2005-06-16
AU2002353465B2 (en) 2008-10-16
US8377692B2 (en) 2013-02-19
EP1456355A2 (en) 2004-09-15
JP2005519585A (en) 2005-07-07
EP1456355A4 (en) 2005-05-25
CA2467046C (en) 2011-07-19
AU2002353465A1 (en) 2003-06-10
NZ533033A (en) 2006-01-27
KR20040076860A (en) 2004-09-03
IL162094A (en) 2011-09-27
CN1615357A (en) 2005-05-11
KR101001119B1 (en) 2010-12-14
CA2467046A1 (en) 2003-05-30

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