WO2003051927A3 - Production of f(ab')2 fragments in mammalian cells - Google Patents

Production of f(ab')2 fragments in mammalian cells Download PDF

Info

Publication number
WO2003051927A3
WO2003051927A3 PCT/NL2002/000841 NL0200841W WO03051927A3 WO 2003051927 A3 WO2003051927 A3 WO 2003051927A3 NL 0200841 W NL0200841 W NL 0200841W WO 03051927 A3 WO03051927 A3 WO 03051927A3
Authority
WO
WIPO (PCT)
Prior art keywords
fragments
production
mammalian cells
cells
antibody fragments
Prior art date
Application number
PCT/NL2002/000841
Other languages
French (fr)
Other versions
WO2003051927A2 (en
Inventor
Abraham Bout
David Halford Ashton Jones
Original Assignee
Crucell Holland Bv
Abraham Bout
David Halford Ashton Jones
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holland Bv, Abraham Bout, David Halford Ashton Jones filed Critical Crucell Holland Bv
Priority to NZ533332A priority Critical patent/NZ533332A/en
Priority to SI200230701T priority patent/SI1456238T1/en
Priority to US10/499,298 priority patent/US20050048038A1/en
Priority to CA2470579A priority patent/CA2470579C/en
Priority to DE60226896T priority patent/DE60226896D1/en
Priority to AU2002353662A priority patent/AU2002353662B2/en
Priority to EP02789019A priority patent/EP1456238B1/en
Priority to DK02789019T priority patent/DK1456238T3/en
Publication of WO2003051927A2 publication Critical patent/WO2003051927A2/en
Publication of WO2003051927A3 publication Critical patent/WO2003051927A3/en
Priority to US12/070,145 priority patent/US7537916B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/624—Disulfide-stabilized antibody (dsFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention provides immortalized eucaryotic cells and methods useful for the production of immunologically active bivalent antibody fragments, such as F(ab')2 fragments. The methods and cells of the invention result in a desirable ratio of bivalent to monovalent antibody fragments.
PCT/NL2002/000841 2001-12-17 2002-12-17 Production of f(ab')2 fragments in mammalian cells WO2003051927A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
NZ533332A NZ533332A (en) 2001-12-17 2002-12-17 Efficient production of F(AB')2 fragments in mammalian cells
SI200230701T SI1456238T1 (en) 2001-12-17 2002-12-17 Production of f(ab')2 fragments in mammalian cells
US10/499,298 US20050048038A1 (en) 2001-12-17 2002-12-17 Efficient production of f(ab')2 fragments in mammalian cells
CA2470579A CA2470579C (en) 2001-12-17 2002-12-17 Production of f(ab')2 fragments in mammalian cells
DE60226896T DE60226896D1 (en) 2001-12-17 2002-12-17 PREPARATION OF F (AB ') 2 FRAGMENTS IN TISSUE CELL
AU2002353662A AU2002353662B2 (en) 2001-12-17 2002-12-17 Production of F(ab')2 fragments in mammalian cells
EP02789019A EP1456238B1 (en) 2001-12-17 2002-12-17 Production of f(ab')2 fragments in mammalian cells
DK02789019T DK1456238T3 (en) 2001-12-17 2002-12-17 Production of F (ab ') 2 fragments in mammalian cells
US12/070,145 US7537916B2 (en) 2001-12-17 2008-02-14 Efficient production of F(ab')2 fragments in mammalian cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL0100917 2001-12-17
NLPCT/NL01/00917 2001-12-17

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10499298 A-371-Of-International 2002-12-17
US12/070,145 Continuation US7537916B2 (en) 2001-12-17 2008-02-14 Efficient production of F(ab')2 fragments in mammalian cells

Publications (2)

Publication Number Publication Date
WO2003051927A2 WO2003051927A2 (en) 2003-06-26
WO2003051927A3 true WO2003051927A3 (en) 2003-11-20

Family

ID=19760784

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2002/000841 WO2003051927A2 (en) 2001-12-17 2002-12-17 Production of f(ab')2 fragments in mammalian cells

Country Status (11)

Country Link
US (2) US20050048038A1 (en)
EP (1) EP1456238B1 (en)
AT (1) ATE397019T1 (en)
AU (1) AU2002353662B2 (en)
CA (1) CA2470579C (en)
DE (1) DE60226896D1 (en)
DK (1) DK1456238T3 (en)
ES (1) ES2307807T3 (en)
NZ (1) NZ533332A (en)
SI (1) SI1456238T1 (en)
WO (1) WO2003051927A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8236561B2 (en) 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7604960B2 (en) 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7521220B2 (en) 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
US7192759B1 (en) 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7527961B2 (en) 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
ATE384785T1 (en) 2001-12-07 2008-02-15 Crucell Holland Bv PRODUCTION OF VIRUSES, VIRUS ISOLATES, AND VACCINES
DE60226896D1 (en) 2001-12-17 2008-07-10 Crucell Holland Bv PREPARATION OF F (AB ') 2 FRAGMENTS IN TISSUE CELL
JP4865539B2 (en) 2003-05-09 2012-02-01 クルセル ホランド ベー ヴェー E1 immortalized cell culture and method of culturing the culture to increase the yield of product obtained from the culture
WO2019070108A1 (en) 2017-10-02 2019-04-11 Laboratorios Silanes S.A. De C.V. High-performance process for producing poison-antidotes using f(ab')2 antibody fragments
WO2023205656A2 (en) * 2022-04-18 2023-10-26 John Paul Ii Medical Research Institute Immortalized cells for production of bioproducts, methods of producing bioproducts with an immortalized cell, and methods of making immortalized cells for production of bioproducts

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006217A1 (en) * 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES
US5591828A (en) * 1989-06-22 1997-01-07 Behringwerke Aktiengesellschaft Bispecific and oligospecific mono-and oligovalent receptors, the preparation and use thereof
EP1108787A2 (en) * 1999-11-26 2001-06-20 Crucell Holland B.V. Production of vaccines

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4624846A (en) * 1983-07-29 1986-11-25 Immunomedics, Inc. Method for enhancing target specificity of antibody localization and clearance of non-target diagnostic and therapeutic principles
DE3584341D1 (en) * 1984-08-24 1991-11-14 Upjohn Co RECOMBINANT DNA COMPOUNDS AND EXPRESSION OF POLYPEPTIDES LIKE TPA.
US5168062A (en) * 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
DE69638058D1 (en) * 1995-06-15 2009-11-26 Crucell Holland Bv Packaging systems for human recombinant adenoviruses for gene therapy
SI1161548T2 (en) * 1999-04-15 2010-02-26 Crucell Holland Bv Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
EP1130099A1 (en) * 2000-02-25 2001-09-05 Crucell Holland B.V. Activated vitronectin as a marker of angiogenesis detected with phage antibodies
DE60226896D1 (en) 2001-12-17 2008-07-10 Crucell Holland Bv PREPARATION OF F (AB ') 2 FRAGMENTS IN TISSUE CELL

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591828A (en) * 1989-06-22 1997-01-07 Behringwerke Aktiengesellschaft Bispecific and oligospecific mono-and oligovalent receptors, the preparation and use thereof
WO1993006217A1 (en) * 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES
EP1108787A2 (en) * 1999-11-26 2001-06-20 Crucell Holland B.V. Production of vaccines

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOSHART M ET AL: "A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALOVIRUS", CELL, MIT PRESS, CAMBRIDGE, MA,, US, vol. 41, no. 2, June 1985 (1985-06-01), pages 521 - 530, XP001079009, ISSN: 0092-8674 *
FALLAUX F ET AL: "New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses", HUMAN GENE THERAPY, XX, XX, vol. 9, no. 9, 1 September 1998 (1998-09-01), pages 1909 - 1917, XP002111070, ISSN: 1043-0342 *
LEUNG S O ET AL: "The effects of domain deletion, glycosylation, and long IgG3 hinge on the biodistribution and serum stability properties of a humanized IgG1 immunoglobulin, hLL2, and its fragments.", CLINICAL CANCER RESEARCH: AN OFFICIAL JOURNAL OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH. UNITED STATES OCT 1999, vol. 5, no. 10 Suppl, October 1999 (1999-10-01), pages 3106s - 3117s, XP001149244, ISSN: 1078-0432 *

Also Published As

Publication number Publication date
AU2002353662B2 (en) 2009-04-23
DE60226896D1 (en) 2008-07-10
CA2470579C (en) 2012-03-06
ES2307807T3 (en) 2008-12-01
WO2003051927A2 (en) 2003-06-26
US20080166767A1 (en) 2008-07-10
EP1456238B1 (en) 2008-05-28
EP1456238A2 (en) 2004-09-15
ATE397019T1 (en) 2008-06-15
SI1456238T1 (en) 2008-08-31
CA2470579A1 (en) 2003-06-26
DK1456238T3 (en) 2008-08-18
US20050048038A1 (en) 2005-03-03
AU2002353662A1 (en) 2003-06-30
US7537916B2 (en) 2009-05-26
NZ533332A (en) 2005-07-29

Similar Documents

Publication Publication Date Title
IL162648A0 (en) Cellular compositions and methods of making and using them
EP1362093A4 (en) Rapid production of monoclonal antibodies
WO2003051927A3 (en) Production of f(ab')2 fragments in mammalian cells
AU2001294198A1 (en) Cells producing antibody compositions
CA2442135A1 (en) Methods to culture circovirus
NO20061470L (en) Process for the preparation of gamma-carboxylated proteins
IT1293068B1 (en) PROCEDURE FOR OBTAINING A HIGH CONCENTRATION COLLOIDAL SILICA SUSPENSION AND PRODUCT SO OBTAINED
AU1593001A (en) Recombinant gelatin in vaccines
WO2002029083A3 (en) Industrial-scale serum-free production of recombinant proteins in mammalian cells
HK166495A (en) HIV-1 envelope muteins lacking hypervariable domains
CA2188165A1 (en) Methods for proliferating and differentiating b cells, and uses thereof
CA2374003A1 (en) Improved cellular uptake of bioactive agents
HUP0301766A2 (en) Process for the preparation of latent antithrombin iii
AU4017501A (en) Cell culture spinner flasks
PT1451302E (en) In vitro reconstituted sheets of human corneal epithelium and method of producing the same
GB2351296A (en) Keratinocyte stem cells
AU2001283132A1 (en) Enhancement of industrial production by increasing substrate transport
AU2003273521A1 (en) Production method of iturin a and its homologues
AU2001280368A1 (en) Use of anti-ceacam antibodies for stimulating b cells in the production of monoclonal antibodies or in immunotherapy
AU2002337697A1 (en) Pine cone extracts and uses thereof
WO2000073420A3 (en) Creation of human tumorigenic cells and uses therefor
AU5045493A (en) Process for preparing conjugates consisting of a specific binding partner and a carbohydrate-containing protein
BR9800928A (en) Cosmetic material, and, process for preparing the same.
WO2003027242A3 (en) Fas ligand
IT1302261B1 (en) ENZYMATIC KINETIC RESOLUTION PROCESS OF 3-PHENYLGLYCIDATES FOR TRANSESTERIFICATION WITH AMINO-ALCOHOLS

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2002789019

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002353662

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 533332

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 2470579

Country of ref document: CA

WWP Wipo information: published in national office

Ref document number: 2002789019

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10499298

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 533332

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 533332

Country of ref document: NZ

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP