WO2003062462A3 - Method for isolating nucleic acids and protein from a single sample - Google Patents

Method for isolating nucleic acids and protein from a single sample Download PDF

Info

Publication number
WO2003062462A3
WO2003062462A3 PCT/GB2003/000156 GB0300156W WO03062462A3 WO 2003062462 A3 WO2003062462 A3 WO 2003062462A3 GB 0300156 W GB0300156 W GB 0300156W WO 03062462 A3 WO03062462 A3 WO 03062462A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
isolating nucleic
nucleic acid
nucleic acids
single sample
Prior art date
Application number
PCT/GB2003/000156
Other languages
French (fr)
Other versions
WO2003062462A2 (en
Inventor
Marie Bosnes
Original Assignee
Dynal Biotech Asa
Marie Bosnes
Dzieglewska Hanna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0200927A external-priority patent/GB0200927D0/en
Priority claimed from GB0227239A external-priority patent/GB0227239D0/en
Application filed by Dynal Biotech Asa, Marie Bosnes, Dzieglewska Hanna filed Critical Dynal Biotech Asa
Priority to EP03700880A priority Critical patent/EP1468116A2/en
Priority to US10/501,162 priority patent/US8110351B2/en
Priority to JP2003562329A priority patent/JP2005538929A/en
Priority to AU2003202026A priority patent/AU2003202026A1/en
Priority to CA002473376A priority patent/CA2473376A1/en
Publication of WO2003062462A2 publication Critical patent/WO2003062462A2/en
Publication of WO2003062462A3 publication Critical patent/WO2003062462A3/en
Priority to US13/343,503 priority patent/US20120178090A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The present invention comprises a method of isolating nucleic acid and protein from the same sample with solid supports, wherein nucleic acid and protein components contained in the sample become bound to distinct solid supports. The invention also allows for kits for isolating nucleic acid and protein from the same sample and for use of the method of isolating nucleic acid and protein for the analysis and/or comparison of mRNA and/or protein expression and/or their correlation to genomic information.
PCT/GB2003/000156 2002-01-16 2003-01-16 Method for isolating nucleic acids and protein from a single sample WO2003062462A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP03700880A EP1468116A2 (en) 2002-01-16 2003-01-16 Method for isolating nucleic acids and protein from a single sample
US10/501,162 US8110351B2 (en) 2002-01-16 2003-01-16 Method for isolating nucleic acids and protein from a single sample
JP2003562329A JP2005538929A (en) 2002-01-16 2003-01-16 Methods for isolating nucleic acids and proteins from a single sample
AU2003202026A AU2003202026A1 (en) 2002-01-16 2003-01-16 Method for isolating nucleic acids and protein from a single sample
CA002473376A CA2473376A1 (en) 2002-01-16 2003-01-16 Method for isolating nucleic acids and protein from a single sample
US13/343,503 US20120178090A1 (en) 2002-01-16 2012-01-04 Method for isolating nucleic acids and protein from a single sample

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0200927A GB0200927D0 (en) 2002-01-16 2002-01-16 Method
GB0200927.2 2002-01-16
GB0227239.1 2002-11-21
GB0227239A GB0227239D0 (en) 2002-11-21 2002-11-21 DNA/RNA and protection isolation from a single sample

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/343,503 Continuation US20120178090A1 (en) 2002-01-16 2012-01-04 Method for isolating nucleic acids and protein from a single sample

Publications (2)

Publication Number Publication Date
WO2003062462A2 WO2003062462A2 (en) 2003-07-31
WO2003062462A3 true WO2003062462A3 (en) 2003-12-11

Family

ID=27614783

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/000156 WO2003062462A2 (en) 2002-01-16 2003-01-16 Method for isolating nucleic acids and protein from a single sample

Country Status (7)

Country Link
US (2) US8110351B2 (en)
EP (1) EP1468116A2 (en)
JP (1) JP2005538929A (en)
CN (1) CN1617938A (en)
AU (1) AU2003202026A1 (en)
CA (1) CA2473376A1 (en)
WO (1) WO2003062462A2 (en)

Families Citing this family (134)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
JP4831725B2 (en) * 2004-02-13 2011-12-07 栄研化学株式会社 Simple nucleic acid extraction method
EP2261650A3 (en) * 2004-09-15 2011-07-06 IntegenX Inc. Microfluidic devices
US7745207B2 (en) 2006-02-03 2010-06-29 IntegenX, Inc. Microfluidic devices
DE102005015005A1 (en) * 2005-04-01 2006-10-05 Qiagen Gmbh Process for treating a sample containing biomolecules
CN100374857C (en) * 2006-01-26 2008-03-12 复旦大学 Method for fast enriching trace polypeptide and protein and realizing identification
US7770107B2 (en) * 2006-03-01 2010-08-03 Oracle International Corporation Methods and systems for extracting and processing translatable and transformable data from XSL files
KR100829585B1 (en) * 2006-04-07 2008-05-14 삼성전자주식회사 Method and apparatus for target cell separation and rapid nucleic acids isolation
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
WO2009032781A2 (en) * 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
WO2009058997A2 (en) * 2007-11-01 2009-05-07 Biocept Inc. Non-invasive isolation of fetal nucleic acid
US8067580B2 (en) 2008-02-07 2011-11-29 Ge Healthcare Bio-Sciences Corp. Isolation of DNA, RNA and protein from a single sample
EP2260112A4 (en) * 2008-04-05 2013-10-09 Single Cell Technology Inc Method of screening single cells for the production of biologically active agents
WO2009137059A1 (en) * 2008-05-05 2009-11-12 Los Alamos National Security, Llc Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
EP2370561B1 (en) 2008-12-16 2019-08-07 EMD Millipore Corporation Stirred tank reactor and method
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
AU2010256429B2 (en) 2009-06-05 2015-09-17 Integenx Inc. Universal sample preparation system and use in an integrated analysis system
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
ES2754210T3 (en) 2010-05-17 2020-04-16 Emd Millipore Corp Stimulus-sensitive polymers for biomolecule purification
DK2588609T3 (en) 2010-06-29 2018-03-19 Exscale Biospecimen Solutions Ab METHOD AND KIT FOR SEQUENTIAL ISOLATION OF NUCLEOTIDE SPECIES FROM A SAMPLE
US8747084B2 (en) 2010-07-21 2014-06-10 Aperia Technologies, Inc. Peristaltic pump
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US9719893B2 (en) 2011-11-28 2017-08-01 Syngenta Participations Ag DNA extraction from seeds using osmoticum
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
ES2619629T3 (en) 2012-03-20 2017-06-26 Aperia Technologies Tire inflation system
WO2013145431A1 (en) * 2012-03-30 2013-10-03 株式会社日立製作所 METHOD FOR AMPLIFYING cDNA DERIVED FROM TRACE AMOUNT OF SAMPLE
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN113528634A (en) 2012-08-14 2021-10-22 10X基因组学有限公司 Microcapsule compositions and methods
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10245908B2 (en) 2016-09-06 2019-04-02 Aperia Technologies, Inc. System for tire inflation
US11453258B2 (en) 2013-03-12 2022-09-27 Aperia Technologies, Inc. System for tire inflation
US10144254B2 (en) 2013-03-12 2018-12-04 Aperia Technologies, Inc. Tire inflation system
US9604157B2 (en) 2013-03-12 2017-03-28 Aperia Technologies, Inc. Pump with water management
CN105378108A (en) * 2013-03-13 2016-03-02 雅培分子公司 Systems and methods for isolating nucleic acids
ES2865124T3 (en) * 2013-03-15 2021-10-15 Abbott Molecular Inc One-step procedure for the purification of nucleic acids
KR20150017611A (en) * 2013-08-07 2015-02-17 삼성전자주식회사 Method for separation of nucleic acid from cells
KR102536833B1 (en) 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
MX2016013156A (en) 2014-04-10 2017-02-14 10X Genomics Inc Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same.
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
WO2015191508A1 (en) 2014-06-09 2015-12-17 Voyager Therapeutics, Inc. Chimeric capsids
CN113249435A (en) 2014-06-26 2021-08-13 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
US10690627B2 (en) 2014-10-22 2020-06-23 IntegenX, Inc. Systems and methods for sample preparation, processing and analysis
AU2015339148B2 (en) 2014-10-29 2022-03-10 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CA2966620A1 (en) 2014-11-05 2016-05-12 Voyager Therapeutics, Inc. Aadc polynucleotides for the treatment of parkinson's disease
CN112375760A (en) 2014-11-14 2021-02-19 沃雅戈治疗公司 Regulatory polynucleotides
CN114717264A (en) 2014-11-14 2022-07-08 沃雅戈治疗公司 Compositions and methods for treating Amyotrophic Lateral Sclerosis (ALS)
EP3230441A4 (en) 2014-12-12 2018-10-03 Voyager Therapeutics, Inc. Compositions and methods for the production of scaav
KR102321863B1 (en) 2015-01-12 2021-11-08 10엑스 제노믹스, 인크. Method and system for preparing nucleic acid sequencing library and library prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
EP3936619A1 (en) 2015-02-24 2022-01-12 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
WO2017027620A1 (en) 2015-08-10 2017-02-16 Life Technologies Corporation Devices and methods for laser capture microdissection
CN108026524A (en) 2015-09-11 2018-05-11 赛卢拉研究公司 Method and composition for nucleic acid library standardization
US20180230489A1 (en) 2015-10-28 2018-08-16 Voyager Therapeutics, Inc. Regulatable expression using adeno-associated virus (aav)
EP3882357B1 (en) 2015-12-04 2022-08-10 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
CN105524918B (en) * 2016-03-02 2018-06-15 四川农业大学 A kind of method of high-throughput extraction gardening plant leaves genomic DNA
EP3448874A4 (en) 2016-04-29 2020-04-22 Voyager Therapeutics, Inc. Compositions for the treatment of disease
WO2017189964A2 (en) 2016-04-29 2017-11-02 Voyager Therapeutics, Inc. Compositions for the treatment of disease
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
BR112018073472A2 (en) 2016-05-18 2019-08-27 Voyager Therapeutics Inc Huntington's disease treatment compositions and methods
EP3458588A4 (en) 2016-05-18 2020-01-15 Voyager Therapeutics, Inc. Modulatory polynucleotides
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11298041B2 (en) 2016-08-30 2022-04-12 The Regents Of The University Of California Methods for biomedical targeting and delivery and devices and systems for practicing the same
JP7091348B2 (en) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183A3 (en) 2017-01-30 2024-02-21 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification is carried out using blocking property oligonucleotides
CN108427000B (en) * 2017-02-15 2021-06-08 广州市锐博生物科技有限公司 Method and kit for capturing nucleic acid binding protein
JP2020518259A (en) 2017-05-05 2020-06-25 ボイジャー セラピューティクス インコーポレイテッドＶｏｙａｇｅｒ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． Huntington's disease treatment compositions and methods
AU2018261790A1 (en) 2017-05-05 2019-11-28 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (ALS)
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN110719959B (en) 2017-06-05 2021-08-06 贝克顿迪金森公司 Sample indexing for single cells
JOP20190269A1 (en) 2017-06-15 2019-11-20 Voyager Therapeutics Inc Aadc polynucleotides for the treatment of parkinson's disease
CN111132626B (en) 2017-07-17 2024-01-30 沃雅戈治疗公司 Track array guidance system
JP7221275B2 (en) 2017-08-03 2023-02-13 ボイジャー セラピューティクス インコーポレイテッド Compositions and methods for delivering AAV
US11434502B2 (en) 2017-10-16 2022-09-06 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis (ALS)
US20200237799A1 (en) 2017-10-16 2020-07-30 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis (als)
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US20210010028A1 (en) 2018-03-06 2021-01-14 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes
CA3091171A1 (en) * 2018-04-06 2019-10-10 Boehringer Ingelheim Vetmedica Gmbh Method for determining an analyte, and analysis system
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US20210214749A1 (en) 2018-05-16 2021-07-15 Voyager Therapeutics, Inc. Directed evolution
WO2019222441A1 (en) 2018-05-16 2019-11-21 Voyager Therapeutics, Inc. Aav serotypes for brain specific payload delivery
CA3103963A1 (en) 2018-07-02 2020-01-09 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis and disorders associated with the spinal cord
US20210355454A1 (en) 2018-07-24 2021-11-18 Voyager Therapeutics, Inc. Systems and methods for producing gene therapy formulations
JP2022511398A (en) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー Determining the 5'transcription sequence
WO2020072849A1 (en) 2018-10-04 2020-04-09 Voyager Therapeutics, Inc. Methods for measuring the titer and potency of viral vector particles
JP2022506546A (en) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
WO2020112686A1 (en) 2018-11-27 2020-06-04 Aperia Technologies, Inc. Hub-integrated inflation system
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
JP2022522995A (en) 2019-01-18 2022-04-21 ボイジャー セラピューティクス インコーポレイテッド Methods and systems for producing AAV particles
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US20220243225A1 (en) 2019-04-29 2022-08-04 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US20220290182A1 (en) 2019-08-09 2022-09-15 Voyager Therapeutics, Inc. Cell culture medium for use in producing gene therapy products in bioreactors
US20220364114A1 (en) 2019-08-26 2022-11-17 Voyager Therapeutics, Inc. Controlled expression of viral proteins
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US20230295656A1 (en) 2020-08-06 2023-09-21 Voyager Therapeutics, Inc. Cell culture medium for use in producing gene therapy products in bioreactors
CN114426965B (en) * 2020-10-29 2023-12-26 深圳华大生命科学研究院 Kit for simultaneously extracting DNA, RNA and protein from plant tissue sample and general method
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
WO2022187548A1 (en) 2021-03-03 2022-09-09 Voyager Therapeutics, Inc. Controlled expression of viral proteins
TW202246516A (en) 2021-03-03 2022-12-01 美商航海家醫療公司 Controlled expression of viral proteins
CN115326937A (en) * 2021-05-11 2022-11-11 山东省食品药品检验研究院 Solid-phase probe for capturing genotoxic impurities and using method and application thereof
CN114525274A (en) * 2022-03-22 2022-05-24 合肥欧创基因生物科技有限公司 Lysate and centrifugation column method virus nucleic acid extraction kit based on lysate
WO2024054983A1 (en) 2022-09-08 2024-03-14 Voyager Therapeutics, Inc. Controlled expression of viral proteins

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4237381C1 (en) * 1992-11-05 1994-04-28 Diagen Inst Molekularbio Carrier material for the simultaneous binding of genotypic and phenotypic substances
EP0885958A1 (en) * 1996-02-25 1998-12-23 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
WO2000029848A1 (en) * 1998-11-17 2000-05-25 Proteo Tools Separation, screening, and identification of biological targets
WO2000042432A1 (en) * 1999-01-18 2000-07-20 Thermo Labsystems Oy Purification process using magnetic particles
WO2000061806A2 (en) * 1999-04-09 2000-10-19 Arcturus Engineering, Inc. GENERIC cDNA OR PROTEIN ARRAY FOR CUSTOMIZED ASSAYS

Family Cites Families (190)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US366438A (en) 1887-07-12 Beauchamp tower
US873102A (en) 1907-06-21 1907-12-10 William W Sly Clutch.
SE328534B (en) 1965-08-27 1970-09-21 Astra Ab
US4055469A (en) 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
JPS598164B2 (en) 1977-11-30 1984-02-23 三菱重工業株式会社 Tanning equipment for filter materials such as dust masks
AU530410B2 (en) 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
JPS5940227B2 (en) 1980-06-24 1984-09-28 富士通株式会社 Reactive sputtering method
DE3132491A1 (en) 1980-08-22 1982-07-01 Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad METHOD FOR PERFORMING ANALYTICAL DETERMINATIONS BY MEANS OF THE CHEMILUMINESCENCE METHOD AND APPLICATION OF THE METHOD FOR IMMUNASSAYS
US4379843A (en) 1981-01-26 1983-04-12 Peter Cashion Immobilization of polynucleotides and polypeptides with tritylated polysaccharides
US5055556A (en) 1981-10-06 1991-10-08 The Board Of Trustees Of The Leland Stanford Jr. Univ. Fluorescent conjugates for analysis of molecules and cells
IL65131A0 (en) 1982-02-28 1982-04-30 Yeda Res & Dev Process for the production of agarose-polyaldehyde beads and their biological applications
NO155316C (en) 1982-04-23 1987-03-11 Sintef PROCEDURE FOR MAKING MAGNETIC POLYMER PARTICLES.
US4861705A (en) 1983-01-31 1989-08-29 Yeda Research And Development Company, Ltd. Method for removing components of biological fluids
DE3310337A1 (en) 1983-03-22 1984-09-27 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg METHOD FOR CARRYING OUT HYBRIDIZATION REACTIONS
US4672040A (en) 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4648975A (en) 1983-08-17 1987-03-10 Pedro B. Macedo Process of using improved silica-based chromatographic supports containing additives
US4920061A (en) 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
CA1327568C (en) 1984-07-06 1994-03-08 Jurg Merz Ionically modified polysaccharides, process for their preparation and the use thereof
GB8501671D0 (en) 1985-01-23 1985-02-27 Allen G J Immunoassay
US4897444A (en) 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
US4710472A (en) 1985-09-25 1987-12-01 The United States Of America As Represented By The Secretary Of The Navy Magnetic separation device
US4889916A (en) 1985-11-19 1989-12-26 The Johns Hopkins University Protein label and drug delivery system
US5076950A (en) 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
US4772550A (en) 1986-02-10 1988-09-20 Miles Inc. Heterogeneous specific binding assay employing an aggregatable binding reagent
US4871433A (en) 1986-04-04 1989-10-03 Materials Research Corporation Method and apparatus for improving the uniformity ion bombardment in a magnetron sputtering system
US6881536B1 (en) 1986-04-30 2005-04-19 Bioveris Corporation Particle based electrochemiluminescent assays
FR2600655B1 (en) 1986-06-30 1988-10-21 Centre Nat Rech Scient PROTEIN FRACTION EXTRACTED FROM GROWTH FACTOR ACTIVITIES, OBTAINING AND APPLICATION AS A STIMULATION AND POTENTIALIZING AGENT FOR GROWTH FACTOR ACTIVITIES
US4843012A (en) 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
DE3639949A1 (en) 1986-11-22 1988-06-09 Diagen Inst Molekularbio METHOD FOR SEPARATING LONG CHAIN NUCLEIC ACIDS
US4935342A (en) 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4874813A (en) 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
NO162946C (en) 1987-08-21 1990-03-14 Otto Soerensen DEVICE FOR MAGNETIC SEPARATION OF CELLS.
US5599667A (en) 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
KR960000479B1 (en) 1987-03-02 1996-01-08 젠-프로우브 인코오퍼레이티드 Polycationic supports for nucleic acid purification separation and hybridization
US5079155A (en) 1987-03-02 1992-01-07 E. I. Du Pont De Nemours And Company Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
US4921805A (en) 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
CA1297432C (en) 1987-07-29 1992-03-17 Gulilat Gebeyehu Nucleic acid capture reagent
FR2619568B1 (en) 1987-08-21 1989-07-07 Centre Nat Rech Scient POLYSTYRENE-DERIVED POLYMERS, PROCESSES FOR THE PREPARATION THEREOF AND APPLICATIONS THEREOF FOR THE ANALYSIS AND PURIFICATION OF ORGANIC MOLECULES
US4908318A (en) 1987-09-04 1990-03-13 Integrated Genetics, Inc. Nucleic acid extraction methods
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US4923978A (en) 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
GB8800702D0 (en) 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor
US5183809A (en) 1990-02-15 1993-02-02 The Trustees Of The University Of Pennsylvania/Childrens Hospital Corporation Cyclodextrin polymers and cyclodextrins immobilized on a solid surface
US4875045A (en) 1988-03-09 1989-10-17 Northern Telecom Limited Variable gain encoder apparatus and method
EP0338591A3 (en) 1988-04-21 1991-09-04 Microprobe Corporation Nucleic acid extraction method
US4952519A (en) 1988-05-02 1990-08-28 E. I. Du Pont De Nemours And Company Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group
DE3921498A1 (en) 1988-09-28 1990-03-29 Bayer Ag POLYMER-TIED DYES, METHOD FOR THE PRODUCTION AND USE THEREOF
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US5028696A (en) 1988-10-28 1991-07-02 Torres Anthony R Ion exchange and separation method
US6448091B1 (en) 1988-11-03 2002-09-10 Igen International, Inc. Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5075430A (en) 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
GR1000347B (en) 1988-12-21 1992-06-25 Ortho Diagnostic Systems Inc Method of preparing nucleotide probes using a bridging complement
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (en) 1989-03-23 1990-10-16 Az Univ Amsterdam METHOD AND COMBINATION OF AGENTS FOR INSULATING NUCLEIC ACID.
JP2910929B2 (en) 1989-03-23 1999-06-23 浜松ホトニクス株式会社 Surface modification method for particles composed of magnetic particles
US4957605A (en) 1989-04-17 1990-09-18 Materials Research Corporation Method and apparatus for sputter coating stepped wafers
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5126028A (en) 1989-04-17 1992-06-30 Materials Research Corporation Sputter coating process control method and apparatus
US6010867A (en) 1989-04-19 2000-01-04 Ibiden Co., Ltd. Reagent for biomaterials assay, preparation method thereof, and assay method
DE3916595A1 (en) 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh METHOD FOR NON-RADIOACTIVE MEASURING OF NUCLEIC ACID SYNTHESIS IN EUKARYONTIC CELLS
US5124444A (en) 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
US5032281A (en) 1989-08-09 1991-07-16 Daicel Chemical Industries, Ltd. Separating membrane and separation method
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5084169A (en) * 1989-09-19 1992-01-28 The University Of Colorado Foundation, Inc. Stationary magnetically stabilized fluidized bed for protein separation and purification
US5433847A (en) 1989-11-01 1995-07-18 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Radial flow chromatography
US5641628A (en) 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
US4997932A (en) 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5000635A (en) 1989-11-17 1991-03-19 The Boeing Company Spring-loaded outboard side lock
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
IT1240870B (en) 1990-02-14 1993-12-17 Talent PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF HUMAN GENOMIC DNA
CA2082936C (en) 1990-05-15 2003-09-23 Peter O. G. Arrhenius Fluorescent porphyrin, and fluorescent phthalocyanine-polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes
US5234924A (en) 1990-09-26 1993-08-10 Adir Et Compagnie Benzothiazine and benzothiazole compounds useful as analgesics
AU8951191A (en) 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5234824A (en) 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5654179A (en) 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5204246A (en) 1990-12-26 1993-04-20 Pioneer Hi-Bred International, Inc. Dna isolation method
US5296347A (en) 1991-02-08 1994-03-22 Ciba Corning Diagnostics Corp. Bridge immunoassay
US5491223A (en) 1991-02-28 1996-02-13 Daicel Chemical Industries, Ltd. Polysaccharide derivative and separating agent
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US6251581B1 (en) 1991-05-22 2001-06-26 Dade Behring Marburg Gmbh Assay method utilizing induced luminescence
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
FR2679255B1 (en) 1991-07-17 1993-10-22 Bio Merieux METHOD OF IMMOBILIZING A NUCLEIC FRAGMENT BY PASSIVE FIXING ON A SOLID SUPPORT, SOLID SUPPORT THUS OBTAINED AND ITS USE.
US5329000A (en) 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
AT397510B (en) 1991-11-06 1994-04-25 Wimmer Theodor METHOD FOR PRODUCING FATTY ACID ESTERS OF SHORT-CHAIN ALCOHOLS
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
DE69324716T2 (en) 1992-02-13 1999-09-09 Becton Dickinson Co Celite hydrate and purification of DNA
DE4208645A1 (en) 1992-03-18 1993-09-23 Bayer Ag OPTICAL SOLID PHASE BIOSENSOR BASED ON FLUORESCENT COLOR-MARGINED POLYIONIC LAYERS
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
GB2272391A (en) 1992-11-05 1994-05-18 Gordon Henry Turner Drilling jig
US5672659A (en) 1993-01-06 1997-09-30 Kinerton Limited Ionic molecular conjugates of biodegradable polyesters and bioactive polypeptides
DE69428891T2 (en) 1993-03-29 2002-06-27 Minnesota Mining & Mfg METHOD FOR CONNECTING LIGANDS IN A POROUS CARRIER (e.g. AZLAKTON) AND ITS APPLICATIONS
US5503816A (en) 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
US5438129A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
GB2282138B (en) 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
US5919455A (en) 1993-10-27 1999-07-06 Enzon, Inc. Non-antigenic branched polymer conjugates
US20030044777A1 (en) 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
GB2284208A (en) 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
CN1052291C (en) 1994-08-25 2000-05-10 株式会社伊萨基开发工机 Excavator for vertical bores
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5434270A (en) 1994-09-15 1995-07-18 Eastman Kodak Company Weakly basic polymerizable monomers and polymers prepared therefrom
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5631146A (en) 1995-01-19 1997-05-20 The General Hospital Corporation DNA aptamers and catalysts that bind adenosine or adenosine-5'-phosphates and methods for isolation thereof
US5762903A (en) 1995-03-10 1998-06-09 Korea Atomic Energy Research Institute Radioactive chitosan complex for radiation therapy
CN1094940C (en) 1995-04-28 2002-11-27 第一制药株式会社 Pentacyclic compounds
DE19520398B4 (en) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetic pigment
JP2965131B2 (en) 1995-07-07 1999-10-18 東洋紡績株式会社 Magnetic carrier for nucleic acid binding and nucleic acid isolation method using the same
CA2230443C (en) 1995-08-28 2009-04-07 The University Of Western Australia Process for the production of ultrafine particles
US6677164B1 (en) 1995-11-21 2004-01-13 Novartis Ag Multivalent polymers, processes for their preparation, and their use for preparing biologically active compounds
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
SE9600590D0 (en) 1996-02-19 1996-02-19 Pharmacia Biotech Ab Methods for chromatographic separation of peptides and nucleic acid and new high-affinity ion exchange matrix
SE9601318D0 (en) 1996-04-04 1996-04-04 Pharmacia Biosensor Ab Method for nucleic acid analysis
US5780319A (en) 1996-04-19 1998-07-14 Pasteur Sanofi Diagnostics Immunoassays to detect antiphospholipid antibodies
US5916746A (en) 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay
US5962412A (en) 1996-06-10 1999-10-05 Arqule, Inc. Method of making polymers having specific properties
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
US5874221A (en) 1996-08-28 1999-02-23 The United States Of America As Represented By The Secretary Of Agriculture Species specific method for the PCR detection of phythophthora
CA2214495C (en) 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
US5914367A (en) 1996-10-10 1999-06-22 Biotechnology Research & Development Corp. Polymer protein composites and methods for their preparation and use
CA2702219C (en) 1996-11-06 2013-01-08 Sequenom, Inc. High density immobilization of nucleic acids
US6060246A (en) 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
EP0853123A1 (en) 1997-01-10 1998-07-15 Roche Diagnostics GmbH Purification of DNA by 'cross-flow-filtration'
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5770712A (en) 1997-03-14 1998-06-23 Virginia Tech Intellectual Properties, Inc. Crosslinked hydrogel beads from chitosan
US20040152084A1 (en) 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
US5958788A (en) 1997-05-28 1999-09-28 Nalco Chemical Company Luminol tagged polymers for treatment of industrial systems
AU745185B2 (en) * 1997-06-25 2002-03-14 Promega Corporation Method of isolating RNA
EP0897978A3 (en) 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirconium oxide and related compounds for purification of nucleic acids
DE19739218A1 (en) 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Purification of substances from a biological sample
JP4304348B2 (en) 1997-09-22 2009-07-29 独立行政法人理化学研究所 DNA isolation method
DE19743518A1 (en) 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automated, universally applicable sample preparation method
DE19747572C1 (en) 1997-10-28 1999-04-08 Inst Chemo Biosensorik Apparatus for fluorescence immunoassay
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
ES2301581T3 (en) 1997-12-06 2008-07-01 Invitrogen Corporation ISOLATION OF NUCLEIC ACIDS.
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69941689D1 (en) 1998-02-12 2010-01-07 Univ Texas METHOD AND REAGENTS FOR RAPID AND EFFICIENT INSULATION OF CIRCULATING CANCER CELLS
FR2777355B1 (en) 1998-04-10 2000-05-12 Bio Merieux PROCESS FOR FIXING A BIOLOGICAL MOLECULE ON THE SURFACE OF A SUPPORT CONSISTING OF SILICA OR METAL OXIDE
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US6287772B1 (en) 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
ATE317297T1 (en) * 1998-11-23 2006-02-15 Us Gov Sec Army CLEANING METHOD AND APPARATUS
WO2000044928A2 (en) 1999-01-27 2000-08-03 Halaka Folim G Materials and methods for the purification of polyelectrolytes
CA2360042A1 (en) 1999-01-29 2000-08-03 Amersham Pharmacia Biotech K.K. Temperature-responsive polymer compound and process for producing the same
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
EP1069131B1 (en) 1999-07-15 2006-03-15 Qiagen GmbH Methods for separating particulate substrates from solution while minimizing particle loss
WO2001038585A2 (en) 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
US6582907B1 (en) 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
US6638906B1 (en) 1999-12-13 2003-10-28 Nobex Corporation Amphiphilic polymers and polypeptide conjugates comprising same
JP2001231907A (en) 2000-02-24 2001-08-28 Mori Mokko:Kk Pin setting apparatus
US6489160B2 (en) 2000-03-16 2002-12-03 Kabushiki Kaisha Toshiba Method for producing nucleic acid strand immobilized carrier
US7078536B2 (en) 2001-03-14 2006-07-18 Genesoft Pharmaceuticals, Inc. Charged compounds comprising a nucleic acid binding moiety and uses therefor
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
GB0013658D0 (en) 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
GB0026560D0 (en) 2000-10-30 2000-12-13 Novartis Ag Organic compounds
DE60015360T9 (en) 2000-11-20 2005-12-15 Sony International (Europe) Gmbh Method for the immobilization of nucleic acids on a solid support
US6743586B2 (en) 2001-01-29 2004-06-01 The United States Of America As Represented By The Secretary Of The Commerce Rapid fluorescence detection of binding to nucleic acid drug targets labelled with highly fluorescent nucleotide base analogs
US7018610B2 (en) 2001-03-02 2006-03-28 University Of Western Ontario Polymer precursors of radiolabeled compounds, and methods of making and using the same
JP3975319B2 (en) 2001-03-02 2007-09-12 富士ゼロックス株式会社 Developing device and image forming apparatus using the same
US20040259082A1 (en) 2001-04-24 2004-12-23 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymers
US7338805B2 (en) 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
US7183116B2 (en) 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
WO2003017938A2 (en) 2001-08-22 2003-03-06 Watson Pharmaceuticals, Inc. Conjugates targeted to target receptors
US20030099954A1 (en) 2001-11-26 2003-05-29 Stefan Miltenyi Apparatus and method for modification of magnetically immobilized biomolecules
US20030229013A1 (en) 2001-12-07 2003-12-11 Shih-Kwang Wu Solid phase method for synthesis peptide-spacer-lipid conjugates, conjugates synthesized thereby and targeted liposomes containing the same
EP1369689B1 (en) 2001-12-27 2008-03-26 Matsushita Electric Industrial Co., Ltd. Immunoassay method and immunoassay reagent kit to be used therein
CA2480673A1 (en) 2002-03-29 2003-10-09 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
GB0209539D0 (en) 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
AU2003243722A1 (en) 2002-06-20 2004-01-06 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
ES2278181T3 (en) 2002-09-27 2007-08-01 Dsm Ip Assets B.V. MICROBIAL PRODUCTION OF VITAMIN C.
DE60227525D1 (en) 2002-10-18 2008-08-21 Sloning Biotechnology Gmbh Process for the preparation of nucleic acid molecules
FR2847581B1 (en) 2002-11-21 2007-03-23 Commissariat Energie Atomique METHOD FOR FIXING A PROTEIN ON A POLYMER BASED ON PYRROLE AND ITS USE IN THE MANUFACTURE OF A SENSOR
GB0229287D0 (en) 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
US20050123932A1 (en) 2003-12-09 2005-06-09 Mekbib Astatke Nucleic acid-chelating agent conjugates

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4237381C1 (en) * 1992-11-05 1994-04-28 Diagen Inst Molekularbio Carrier material for the simultaneous binding of genotypic and phenotypic substances
EP0885958A1 (en) * 1996-02-25 1998-12-23 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
WO2000029848A1 (en) * 1998-11-17 2000-05-25 Proteo Tools Separation, screening, and identification of biological targets
WO2000042432A1 (en) * 1999-01-18 2000-07-20 Thermo Labsystems Oy Purification process using magnetic particles
WO2000061806A2 (en) * 1999-04-09 2000-10-19 Arcturus Engineering, Inc. GENERIC cDNA OR PROTEIN ARRAY FOR CUSTOMIZED ASSAYS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BÜSSOW ET AL: "A METHOD FOR GLOBAL PROTEIN EXPRESSION AND ANTIBODY SCREENING ON HIGH-DENSITY FILTERS OF AN ARRAYED cDNA LIBRARY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 21, November 1998 (1998-11-01), pages 5007 - 5008, XP002114084, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
US20120178090A1 (en) 2012-07-12
CN1617938A (en) 2005-05-18
WO2003062462A2 (en) 2003-07-31
US20050239068A1 (en) 2005-10-27
JP2005538929A (en) 2005-12-22
AU2003202026A1 (en) 2003-09-02
US8110351B2 (en) 2012-02-07
EP1468116A2 (en) 2004-10-20
CA2473376A1 (en) 2003-07-31

Similar Documents

Publication Publication Date Title
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
ATE549414T1 (en) METHOD FOR ISOLATION OF CELLS
WO2002079490A3 (en) Bio-barcodes based on oligonucleotide-modified particles
WO2001027624A3 (en) Compositions and methods for detecting protein modification and enzymatic activity
WO2005071110A3 (en) Improving polynucleotide ligation reactions
WO2006078289A3 (en) Bio-barcode based detection of target analytes
WO2003008622A3 (en) Exponential nucleic acid amplification using nicking endonucleases
AU2003212954A1 (en) Skin cell biomarkers and methods for identifying biomarkers using nucleic acid microarrays
WO2005087951A3 (en) Reagents, methods and kits for use in deactivating nucleic acids
WO2004051218A8 (en) Multiplex amplification of polynucleotides
WO2004074310A3 (en) Mycobacterial diagnostics
WO2007086900A3 (en) Stilbazium research assays
AU2002952797A0 (en) Centrifugal device and method using same
ATE314385T1 (en) METHOD FOR OBTAINING NUCLEIC ACIDS USING CHAOTROPERS AND AMMONIUM REAGENTS
WO2004053457A3 (en) Quantitative test for bacterial pathogens
WO2004057016A3 (en) METHODS OF CAPTURING, DETECTING AND QUANTIFYING RNA:DNA HYBRIDS AND A MODIFIED RNase H USEFUL THEREIN
WO2000040757A3 (en) Method of identifying nucleic acids
WO2006037102A3 (en) Methods for identifying polymerase inhibitors
WO2003060119A3 (en) Method for the quantification of in vivo nucleic acid levels
WO2001049886A8 (en) Method of analyzing a nucleic acid
AUPS064202A0 (en) Improved method for the analysis of nucleic acid samples
AU2003303869A1 (en) Methods for nucleic acid analysis
WO2004046382A3 (en) Product and method
EP1323835A3 (en) Method for determining biospecies contained in test specimen and kit used for the same
WO2005001416A3 (en) High throughput assay of lp-pla2 activity

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2473376

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003562329

Country of ref document: JP

Ref document number: 20038023733

Country of ref document: CN

WWE Wipo information: entry into national phase

Ref document number: 2003700880

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003700880

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10501162

Country of ref document: US