WO2003065976A3 - Microfluidics apparatus and methods of use therefor - Google Patents

Microfluidics apparatus and methods of use therefor Download PDF

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Publication number
WO2003065976A3
WO2003065976A3 PCT/US2002/035990 US0235990W WO03065976A3 WO 2003065976 A3 WO2003065976 A3 WO 2003065976A3 US 0235990 W US0235990 W US 0235990W WO 03065976 A3 WO03065976 A3 WO 03065976A3
Authority
WO
WIPO (PCT)
Prior art keywords
interaction
methods
cells
sample fluid
use therefor
Prior art date
Application number
PCT/US2002/035990
Other languages
French (fr)
Other versions
WO2003065976A2 (en
Inventor
John P Peeters
Thomas Wiggins
Madhushree Ghosh
Lawrence A Bottomley
Salvatore Seminara
Zhiyu Hu
Timothy Seeley
Sebastian Kossek
Original Assignee
Protiveris Inc
John P Peeters
Thomas Wiggins
Madhushree Ghosh
Lawrence A Bottomley
Salvatore Seminara
Zhiyu Hu
Timothy Seeley
Sebastian Kossek
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US10/054,760 priority Critical patent/US6926864B2/en
Application filed by Protiveris Inc, John P Peeters, Thomas Wiggins, Madhushree Ghosh, Lawrence A Bottomley, Salvatore Seminara, Zhiyu Hu, Timothy Seeley, Sebastian Kossek filed Critical Protiveris Inc
Priority to AU2002365434A priority patent/AU2002365434A1/en
Priority to CA002464880A priority patent/CA2464880A1/en
Priority to EP02805704A priority patent/EP1487960A2/en
Priority to JP2003565402A priority patent/JP2005517159A/en
Publication of WO2003065976A2 publication Critical patent/WO2003065976A2/en
Publication of WO2003065976A3 publication Critical patent/WO2003065976A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis

Abstract

A microfluidics device includes a plurality of interaction cells and fluid control means including i) means for providing to each of the plurality of interaction cells one or more preparation fluids, and ii) means for providing to each of the interaction cells a sample fluid, wherein each interaction cell receives a different sample fluid. A plurality of microcantilevers may be disposed in each of the interaction cells, wherein each of the plurality of microcantilevers configured to deflect in response to an interaction involving a component of the sample fluid.
PCT/US2002/035990 2001-11-09 2002-11-08 Microfluidics apparatus and methods of use therefor WO2003065976A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/054,760 US6926864B2 (en) 2001-11-09 2001-11-13 Microfluidics apparatus and methods for use thereof
AU2002365434A AU2002365434A1 (en) 2001-11-09 2002-11-08 Microfluidics apparatus and methods of use therefor
CA002464880A CA2464880A1 (en) 2001-11-09 2002-11-08 Microfluidics apparatus and methods of use therefor
EP02805704A EP1487960A2 (en) 2001-11-09 2002-11-08 Microfluidics apparatus and methods of use therefor
JP2003565402A JP2005517159A (en) 2001-11-09 2002-11-08 Microfluidic device and method of using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3673301A 2001-11-09 2001-11-09
US10/036,733 2001-11-09
US10/054,760 US6926864B2 (en) 2001-11-09 2001-11-13 Microfluidics apparatus and methods for use thereof
US10/054,760 2001-11-13

Publications (2)

Publication Number Publication Date
WO2003065976A2 WO2003065976A2 (en) 2003-08-14
WO2003065976A3 true WO2003065976A3 (en) 2004-09-30

Family

ID=27736746

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/035990 WO2003065976A2 (en) 2001-11-09 2002-11-08 Microfluidics apparatus and methods of use therefor

Country Status (6)

Country Link
US (2) US6926864B2 (en)
EP (1) EP1487960A2 (en)
JP (1) JP2005517159A (en)
AU (1) AU2002365434A1 (en)
CA (1) CA2464880A1 (en)
WO (1) WO2003065976A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014823A1 (en) * 1999-08-19 2001-03-01 The Regents Of The University Of California Apparatus and method for visually identifying micro-forces with a palette of cantilever array blocks
US7387889B2 (en) * 2002-08-22 2008-06-17 Massachusetts Institute Of Technology Measurement of concentrations and binding energetics
AU2003291417A1 (en) 2002-11-08 2004-06-03 Protiveris, Inc. Multiplex illuminator and device reader for microcantilever array
US7344678B2 (en) * 2002-11-15 2008-03-18 The Regents Of The University Of California Composite sensor membrane
WO2004046689A2 (en) * 2002-11-15 2004-06-03 The Regents Of The University Of California System and method for multiplexed biomolecular analysis
US20050255448A1 (en) * 2004-05-13 2005-11-17 Arun Majumdar System for amplifying optical detection of cantilever deflection
DE102004058828B4 (en) * 2004-10-28 2010-08-19 Progen Biotechnik Gmbh Apparatus and method for parallel processing of biopolymers
WO2006128472A1 (en) * 2005-06-02 2006-12-07 Danmarks Tekniske Universitet Sensor system with actuated structure releasing a coloured indicator
US20080179255A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Fluidic devices
US8068991B2 (en) 2005-11-30 2011-11-29 The Invention Science Fund I, Llc Systems and methods for transmitting pathogen related information and responding
US20080178692A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Fluidic methods
US20080241909A1 (en) * 2007-03-27 2008-10-02 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Microfluidic chips for pathogen detection
US20080241000A1 (en) * 2007-03-27 2008-10-02 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Systems for pathogen detection
US20080241910A1 (en) * 2007-03-27 2008-10-02 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Devices for pathogen detection
US20080181821A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Microfluidic chips for allergen detection
US10001496B2 (en) 2007-01-29 2018-06-19 Gearbox, Llc Systems for allergen detection
US20090050569A1 (en) * 2007-01-29 2009-02-26 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Fluidic methods
US8617903B2 (en) 2007-01-29 2013-12-31 The Invention Science Fund I, Llc Methods for allergen detection
US20080245740A1 (en) * 2007-01-29 2008-10-09 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Fluidic methods
US8168120B1 (en) 2007-03-06 2012-05-01 The Research Foundation Of State University Of New York Reliable switch that is triggered by the detection of a specific gas or substance
US20090227005A1 (en) * 2007-03-27 2009-09-10 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Methods for pathogen detection
CA2635460A1 (en) * 2007-06-21 2008-12-21 Ion Stiharu Methods and devices for early detection of cancer cells and types through micromechanical interactions
WO2009064816A1 (en) 2007-11-13 2009-05-22 The Regents Of The University Of California Processes for rapid microfabrication using thermoplastics and devices thereof
US20090325159A1 (en) * 2008-06-30 2009-12-31 Canon U.S. Life Sciences, Inc. System and method to prevent cross-contamination in assays performed in a microfluidic channel
US8122901B2 (en) 2008-06-30 2012-02-28 Canon U.S. Life Sciences, Inc. System and method for microfluidic flow control
US8939021B2 (en) * 2008-11-18 2015-01-27 Schlumberger Technology Corporation Fluid expansion in mud gas logging
WO2010094323A1 (en) 2009-02-17 2010-08-26 Medimate Holding B.V. An apparatus for the measurement of a concentration of a charged species in a sample
GB0910569D0 (en) * 2009-06-18 2009-07-29 Cambridge Entpr Ltd Fluid sensing, sensor apparatus, systems and methods
US9140684B2 (en) 2011-10-27 2015-09-22 University Of Washington Through Its Center For Commercialization Device to expose cells to fluid shear forces and associated systems and methods
CN104428671B (en) * 2012-05-10 2017-04-26 华盛顿大学商业化中心 Microfluidic devices for measuring platelet coagulation, and associated systems and methods
USD733313S1 (en) * 2013-03-19 2015-06-30 Panasonic Healthcare Holdings Co., Ltd. Chip for biochemical examination
US9671350B2 (en) * 2013-09-04 2017-06-06 Applied Invention, Llc Test mass compensation of mass measurement drift in a microcantilever resonator
US10564122B1 (en) * 2016-10-21 2020-02-18 Iowa State University Research Foundation, Inc. Electrophoretic soil nutrient sensor for agriculture
US10191036B1 (en) * 2018-03-22 2019-01-29 NUB4U, Inc. System for detecting and removing biological analytes in fluids
CN115155688A (en) * 2022-06-24 2022-10-11 北京莱芯生物技术有限公司 Laboratory detects with application of sample liquid-transfering auxiliary operation platform

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6485690B1 (en) * 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
US6523392B2 (en) * 2000-01-25 2003-02-25 Arizona Board Of Regents Microcantilever sensor
US6620625B2 (en) * 2000-01-06 2003-09-16 Caliper Technologies Corp. Ultra high throughput sampling and analysis systems and methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5807758A (en) * 1995-07-21 1998-09-15 Lee; Gil U. Chemical and biological sensor using an ultra-sensitive force transducer
US6118124A (en) * 1996-01-18 2000-09-12 Lockheed Martin Energy Research Corporation Electromagnetic and nuclear radiation detector using micromechanical sensors
US5763768A (en) * 1997-03-17 1998-06-09 Iowa State University Research Foundation, Inc. Analytical method using modified scanning probes
US5923421A (en) * 1997-07-24 1999-07-13 Lockheed Martin Energy Research Corporation Chemical detection using calorimetric spectroscopy
US6123819A (en) * 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
US6096559A (en) * 1998-03-16 2000-08-01 Lockheed Martin Energy Research Corporation Micromechanical calorimetric sensor
US6016686A (en) * 1998-03-16 2000-01-25 Lockheed Martin Energy Research Corporation Micromechanical potentiometric sensors
US6050722A (en) * 1998-03-25 2000-04-18 Thundat; Thomas G. Non-contact passive temperature measuring system and method of operation using micro-mechanical sensors
US5992226A (en) * 1998-05-08 1999-11-30 The United States Of America As Represented By The Secretary Of The Navy Apparatus and method for measuring intermolecular interactions by atomic force microscopy
US6287765B1 (en) * 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6041642A (en) * 1998-06-04 2000-03-28 Lockheed Martin Energy Systems, Inc. Method and apparatus for sensing the natural frequency of a cantilevered body
WO2000066266A1 (en) 1999-05-03 2000-11-09 Cantion A/S Sensor for microfluid handling system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6485690B1 (en) * 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
US6620625B2 (en) * 2000-01-06 2003-09-16 Caliper Technologies Corp. Ultra high throughput sampling and analysis systems and methods
US6523392B2 (en) * 2000-01-25 2003-02-25 Arizona Board Of Regents Microcantilever sensor

Also Published As

Publication number Publication date
EP1487960A2 (en) 2004-12-22
CA2464880A1 (en) 2003-08-14
JP2005517159A (en) 2005-06-09
WO2003065976A2 (en) 2003-08-14
AU2002365434A1 (en) 2003-09-02
US6926864B2 (en) 2005-08-09
US20030092016A1 (en) 2003-05-15
US20050176034A1 (en) 2005-08-11

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