WO2003098208A8 - Molecular topological frationation of macromolecules - Google Patents

Molecular topological frationation of macromolecules

Info

Publication number
WO2003098208A8
WO2003098208A8 PCT/US2003/015148 US0315148W WO03098208A8 WO 2003098208 A8 WO2003098208 A8 WO 2003098208A8 US 0315148 W US0315148 W US 0315148W WO 03098208 A8 WO03098208 A8 WO 03098208A8
Authority
WO
WIPO (PCT)
Prior art keywords
macromolecules
interest
flow
channels
eluant
Prior art date
Application number
PCT/US2003/015148
Other languages
French (fr)
Other versions
WO2003098208A1 (en
Inventor
Patrick B Smith
David M Meunier
Scott A Baker
Homme Robert K Prud
Original Assignee
Dow Global Technologies Inc
Patrick B Smith
David M Meunier
Scott A Baker
Homme Robert K Prud
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Inc, Patrick B Smith, David M Meunier, Scott A Baker, Homme Robert K Prud filed Critical Dow Global Technologies Inc
Priority to JP2004505683A priority Critical patent/JP4564842B2/en
Priority to AU2003243233A priority patent/AU2003243233A1/en
Priority to EP03753030A priority patent/EP1506393A1/en
Priority to US10/510,060 priority patent/US7416698B2/en
Publication of WO2003098208A1 publication Critical patent/WO2003098208A1/en
Publication of WO2003098208A8 publication Critical patent/WO2003098208A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G01N2030/001—Field flow fractionation hydrodynamic fractionation, e.g. CHDF or HDC
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/525—Physical parameters structural properties surface properties, e.g. porosity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/528—Monolithic sorbent material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/44—Resins; rubber; leather

Abstract

A process for characterizing a sample comprising a population of linear macromolecules of interest (104) and a population of long chain branched macromolecules of interest (103), the process including four steps. The first step is to provide a flow through separating medium (100) and a liquid eluant (101) in which the macromolecules of interest dissolve, the separating medium defining flow through channels (102), the eluant flow rate and the average diameter of the channels being in a range so that the linear macromolecules of interest elute before the long chain branched macromolecules of interest (105) (106). The second step is to introduce a sample into the liquid eluant. The third step is to flow the liquid eluant under pressure through the channels (102) of the separating medium (100). The fourth step is to differentiate the linear macromolecules of interest (104) from the long chain branched macromolecules of interest (103) based on their successive elution volumes established in the third step such as by determining the refractive index of the successive elution volumes or by subjecting the successive elution volumes to size exclusion chromatography.
PCT/US2003/015148 2002-05-15 2003-05-14 Molecular topological frationation of macromolecules WO2003098208A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004505683A JP4564842B2 (en) 2002-05-15 2003-05-14 Molecular topological fractionation of polymers
AU2003243233A AU2003243233A1 (en) 2002-05-15 2003-05-14 Molecular topological frationation of macromolecules
EP03753030A EP1506393A1 (en) 2002-05-15 2003-05-14 Molecular topological frationation of macromolecules
US10/510,060 US7416698B2 (en) 2002-05-15 2003-05-14 Molecular topological fractionation of macromolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38113702P 2002-05-15 2002-05-15
US60/381,137 2002-05-15

Publications (2)

Publication Number Publication Date
WO2003098208A1 WO2003098208A1 (en) 2003-11-27
WO2003098208A8 true WO2003098208A8 (en) 2008-01-10

Family

ID=29550073

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/015148 WO2003098208A1 (en) 2002-05-15 2003-05-14 Molecular topological frationation of macromolecules

Country Status (5)

Country Link
US (1) US7416698B2 (en)
EP (1) EP1506393A1 (en)
JP (1) JP4564842B2 (en)
AU (1) AU2003243233A1 (en)
WO (1) WO2003098208A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7514516B2 (en) * 2003-05-27 2009-04-07 Fina Technology, Inc. Measurement and preparation of branched vinyl polymers
ES2632565T3 (en) 2005-01-24 2017-09-14 Dow Global Technologies Llc Apparatus and methods for polymer characterization
ES2572914T3 (en) 2011-06-03 2016-06-03 Dow Global Technologies Llc Polymer chromatography
US10481164B2 (en) 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
CN107407667B (en) 2015-03-10 2021-06-15 昭和电工株式会社 Filler for liquid chromatography

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4112032A (en) 1976-04-21 1978-09-05 Corning Glass Works Silica-containing porous bodies of controlled pore size
US4775943A (en) * 1985-10-16 1988-10-04 The Dow Chemical Company Method and apparatus for determining polymer molecular weight distribution parameters
US6064945A (en) * 1998-02-20 2000-05-16 Waters Investments Limited System and method for determining molecular weight and intrinsic viscosity of a polymeric distribution using gel permeation chromatography
US6294388B1 (en) * 1998-04-03 2001-09-25 Symyx Technologies, Inc. Indirect calibration of polymer characterization systems
EP1195391A1 (en) * 2000-10-05 2002-04-10 ATOFINA Research Production of polypropylene
MY137183A (en) * 2001-03-16 2009-01-30 Dow Global Technologies Inc Method of making interpolymers and products made therefrom

Also Published As

Publication number Publication date
EP1506393A1 (en) 2005-02-16
US20050176154A1 (en) 2005-08-11
JP4564842B2 (en) 2010-10-20
AU2003243233A1 (en) 2003-12-02
WO2003098208A1 (en) 2003-11-27
AU2003243233A8 (en) 2008-03-06
US7416698B2 (en) 2008-08-26
JP2005525576A (en) 2005-08-25

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