WO2004002627B1 - Method and apparatus for fluid dispersion - Google Patents
Method and apparatus for fluid dispersionInfo
- Publication number
- WO2004002627B1 WO2004002627B1 PCT/US2003/020542 US0320542W WO2004002627B1 WO 2004002627 B1 WO2004002627 B1 WO 2004002627B1 US 0320542 W US0320542 W US 0320542W WO 2004002627 B1 WO2004002627 B1 WO 2004002627B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fluid
- subject fluid
- channel
- subject
- sectional dimension
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
- B05B7/02—Spray pistols; Apparatus for discharge
- B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
- B05B7/0408—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing two or more liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/41—Emulsifying
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
- B01F25/452—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces
- B01F25/4521—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces the components being pressed through orifices in elements, e.g. flat plates or cylinders, which obstruct the whole diameter of the tube
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
- B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
- B05B7/02—Spray pistols; Apparatus for discharge
- B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
- B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
- B05B7/02—Spray pistols; Apparatus for discharge
- B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
- B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
- B05B7/0441—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid with one inner conduit of liquid surrounded by an external conduit of gas upstream the mixing chamber
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2215/00—Auxiliary or complementary information in relation with mixing
- B01F2215/04—Technical information in relation with mixing
- B01F2215/0413—Numerical information
- B01F2215/0418—Geometrical information
- B01F2215/0431—Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2215/00—Auxiliary or complementary information in relation with mixing
- B01F2215/04—Technical information in relation with mixing
- B01F2215/0413—Numerical information
- B01F2215/0436—Operational information
- B01F2215/045—Numerical flow-rate values
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
- Y10S516/924—Significant dispersive or manipulative operation or step in making or stabilizing colloid system
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
- Y10S516/924—Significant dispersive or manipulative operation or step in making or stabilizing colloid system
- Y10S516/927—Significant dispersive or manipulative operation or step in making or stabilizing colloid system in situ formation of a colloid system making or stabilizing agent which chemical reaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
- Y10T137/0324—With control of flow by a condition or characteristic of a fluid
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
- Y10T137/0324—With control of flow by a condition or characteristic of a fluid
- Y10T137/0329—Mixing of plural fluids of diverse characteristics or conditions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/206—Flow affected by fluid contact, energy field or coanda effect [e.g., pure fluid device or system]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
- Y10T137/87265—Dividing into parallel flow paths with recombining
- Y10T137/87338—Flow passage with bypass
- Y10T137/87346—Including mixing feature
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49002—Electrical device making
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Abstract
Claims
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004549845A JP2006507921A (en) | 2002-06-28 | 2003-06-03 | Method and apparatus for fluid dispersion |
CA2491564A CA2491564C (en) | 2002-06-28 | 2003-06-30 | Method and apparatus for fluid dispersion |
AU2003253751A AU2003253751B2 (en) | 2002-06-28 | 2003-06-30 | Method and apparatus for fluid dispersion |
EP20030762228 EP1515803A2 (en) | 2002-06-28 | 2003-06-30 | Method and apparatus for fluid dispersion |
CN038204940A CN1678397B (en) | 2002-06-28 | 2003-06-30 | Method and apparatus for fluid dispersion |
US11/024,228 US7708949B2 (en) | 2002-06-28 | 2004-12-28 | Method and apparatus for fluid dispersion |
US12/726,223 US8337778B2 (en) | 2002-06-28 | 2010-03-17 | Method and apparatus for fluid dispersion |
US13/679,190 US8986628B2 (en) | 2002-06-28 | 2012-11-16 | Method and apparatus for fluid dispersion |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39219502P | 2002-06-28 | 2002-06-28 | |
US60/392,195 | 2002-06-28 | ||
US42404202P | 2002-11-05 | 2002-11-05 | |
US60/424,042 | 2002-11-05 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/024,228 Continuation US7708949B2 (en) | 2002-06-28 | 2004-12-28 | Method and apparatus for fluid dispersion |
Publications (4)
Publication Number | Publication Date |
---|---|
WO2004002627A2 WO2004002627A2 (en) | 2004-01-08 |
WO2004002627A3 WO2004002627A3 (en) | 2004-04-01 |
WO2004002627B1 true WO2004002627B1 (en) | 2004-06-17 |
WO2004002627A8 WO2004002627A8 (en) | 2005-03-17 |
Family
ID=30003231
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/020542 WO2004002627A2 (en) | 2002-06-28 | 2003-06-30 | Method and apparatus for fluid dispersion |
Country Status (7)
Country | Link |
---|---|
US (3) | US7708949B2 (en) |
EP (2) | EP1515803A2 (en) |
JP (2) | JP2006507921A (en) |
CN (2) | CN102059162A (en) |
AU (1) | AU2003253751B2 (en) |
CA (1) | CA2491564C (en) |
WO (1) | WO2004002627A2 (en) |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102648053A (en) * | 2009-10-27 | 2012-08-22 | 哈佛学院院长等 | Droplet creation techniques |
US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
US8871444B2 (en) | 2004-10-08 | 2014-10-28 | Medical Research Council | In vitro evolution in microfluidic systems |
US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
US9216414B2 (en) | 2009-11-25 | 2015-12-22 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
US9228229B2 (en) | 2010-02-12 | 2016-01-05 | Raindance Technologies, Inc. | Digital analyte analysis |
US9273308B2 (en) | 2006-05-11 | 2016-03-01 | Raindance Technologies, Inc. | Selection of compartmentalized screening method |
US9364803B2 (en) | 2011-02-11 | 2016-06-14 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
US9410151B2 (en) | 2006-01-11 | 2016-08-09 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
US9440232B2 (en) | 2007-02-06 | 2016-09-13 | Raindance Technologies, Inc. | Manipulation of fluids and reactions in microfluidic systems |
US9448172B2 (en) | 2003-03-31 | 2016-09-20 | Medical Research Council | Selection by compartmentalised screening |
US9498759B2 (en) | 2004-10-12 | 2016-11-22 | President And Fellows Of Harvard College | Compartmentalized screening by microfluidic control |
US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
US9562897B2 (en) | 2010-09-30 | 2017-02-07 | Raindance Technologies, Inc. | Sandwich assays in droplets |
Families Citing this family (398)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006507921A (en) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | Method and apparatus for fluid dispersion |
WO2004071638A2 (en) | 2003-02-11 | 2004-08-26 | Regents Of The University Of California, The | Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles |
GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
EP1610888A2 (en) | 2003-04-10 | 2006-01-04 | President And Fellows Of Harvard College | Formation and control of fluidic species |
BRPI0414004A (en) * | 2003-08-27 | 2006-10-24 | Harvard College | electronic control of fluidic species |
US8696952B2 (en) * | 2004-04-23 | 2014-04-15 | Eugenia Kumacheva | Method of producing polymeric particles with selected size, shape, morphology and composition |
NL1026261C2 (en) * | 2004-05-25 | 2005-11-28 | Nanomi B V | Spraying device with a nozzle plate provided with structures for promoting self-breakup, a nozzle plate, and methods for manufacturing and using such a nozzle plate. |
GB0502398D0 (en) * | 2005-02-04 | 2005-03-16 | Q Chip Ltd | Device and method for producing spherical segmented flow |
US9477233B2 (en) | 2004-07-02 | 2016-10-25 | The University Of Chicago | Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets |
EP2842635A1 (en) * | 2005-01-17 | 2015-03-04 | Universidad de Sevilla | Method and device for the micromixing of fluids using a reflux cell |
WO2006078841A1 (en) * | 2005-01-21 | 2006-07-27 | President And Fellows Of Harvard College | Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles |
EP1859330B1 (en) | 2005-01-28 | 2012-07-04 | Duke University | Apparatuses and methods for manipulating droplets on a printed circuit board |
CA2599683A1 (en) | 2005-03-04 | 2006-09-14 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
US20070054119A1 (en) * | 2005-03-04 | 2007-03-08 | Piotr Garstecki | Systems and methods of forming particles |
JP2006289250A (en) * | 2005-04-08 | 2006-10-26 | Kao Corp | Micro mixer and fluid mixing method using the same |
US20060280029A1 (en) * | 2005-06-13 | 2006-12-14 | President And Fellows Of Harvard College | Microfluidic mixer |
WO2007024778A2 (en) | 2005-08-22 | 2007-03-01 | Applera Corporation | Device, system and method for depositing processed immiscible-fluid-discrete-volumes |
US7709544B2 (en) | 2005-10-25 | 2010-05-04 | Massachusetts Institute Of Technology | Microstructure synthesis by flow lithography and polymerization |
KR100928389B1 (en) * | 2005-10-25 | 2009-11-23 | 메사추세츠 인스티튜트 오브 테크놀로지 | Microstructure Synthesis by Flow Lithography and Polymerization |
GB2433448B (en) | 2005-12-20 | 2011-03-02 | Q Chip Ltd | Method for the control of chemical processes |
JP4713397B2 (en) | 2006-01-18 | 2011-06-29 | 株式会社リコー | Microchannel structure and microdroplet generation system |
DE602007009811D1 (en) | 2006-01-27 | 2010-11-25 | Harvard College | COALESCENCE FLUIDER DROPLET |
JP4987885B2 (en) * | 2006-03-09 | 2012-07-25 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | Apparatus for carrying out a reaction in a droplet and method of use thereof |
CA2648099C (en) * | 2006-03-31 | 2012-05-29 | The Brigham And Women's Hospital, Inc | System for targeted delivery of therapeutic agents |
US20140193807A1 (en) | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US9476856B2 (en) | 2006-04-13 | 2016-10-25 | Advanced Liquid Logic, Inc. | Droplet-based affinity assays |
US8980198B2 (en) * | 2006-04-18 | 2015-03-17 | Advanced Liquid Logic, Inc. | Filler fluids for droplet operations |
US7901947B2 (en) | 2006-04-18 | 2011-03-08 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
US8809068B2 (en) | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US20080243309A2 (en) * | 2006-05-08 | 2008-10-02 | Auburn University | Systems for and methods of characterizing reactions |
WO2009111769A2 (en) | 2008-03-07 | 2009-09-11 | Advanced Liquid Logic, Inc. | Reagent and sample preparation and loading on a fluidic device |
KR100848559B1 (en) * | 2006-06-29 | 2008-07-25 | 엘지디스플레이 주식회사 | FABRICATING METHOD OF SOFT MOLD AND pattern forming METHOD USING THEREOF |
US7892434B2 (en) * | 2006-08-02 | 2011-02-22 | The Regents Of The University Of California | Microfluidic production of monodispersed submicron emulsion through filtration and sorting of satellite drops |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
US7947487B2 (en) | 2006-10-05 | 2011-05-24 | Massachusetts Institute Of Technology | Multifunctional encoded particles for high-throughput analysis |
US9874501B2 (en) | 2006-11-24 | 2018-01-23 | Curiox Biosystems Pte Ltd. | Use of chemically patterned substrate for liquid handling, chemical and biological reactions |
WO2008063135A1 (en) | 2006-11-24 | 2008-05-29 | Agency For Science, Technology And Research | Apparatus for processing a sample in a liquid droplet and method of using the same |
CN101627308B (en) | 2007-02-09 | 2013-08-14 | 先进流体逻辑公司 | Droplet actuator devices and methods employing magnetic beads |
WO2008109176A2 (en) | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
CN102014871A (en) | 2007-03-28 | 2011-04-13 | 哈佛大学 | Emulsions and techniques for formation |
WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
US8691164B2 (en) * | 2007-04-20 | 2014-04-08 | Celula, Inc. | Cell sorting system and methods |
US8476382B2 (en) | 2007-06-05 | 2013-07-02 | Eugenia Kumacheva | Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles |
US20100255556A1 (en) * | 2007-06-29 | 2010-10-07 | President And Fellows Of Harvard College | Methods and apparatus for manipulation of fluidic species |
GB0712861D0 (en) * | 2007-07-03 | 2007-08-08 | Eastman Kodak Co | Continuous ink jet printing of encapsulated droplets |
GB0712863D0 (en) | 2007-07-03 | 2007-08-08 | Eastman Kodak Co | Monodisperse droplet generation |
WO2009011808A1 (en) * | 2007-07-13 | 2009-01-22 | President And Fellows Of Harvard College | Droplet-based selection |
WO2009020633A2 (en) * | 2007-08-07 | 2009-02-12 | President And Fellows Of Harvard College | Metal oxide coating on surfaces |
CN101842159B (en) * | 2007-08-09 | 2014-09-24 | 赛路拉公司 | Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material |
WO2009032863A2 (en) | 2007-09-04 | 2009-03-12 | Advanced Liquid Logic, Inc. | Droplet actuator with improved top substrate |
JP5023902B2 (en) * | 2007-09-06 | 2012-09-12 | 株式会社日立プラントテクノロジー | Emulsifying device |
WO2009039458A2 (en) * | 2007-09-19 | 2009-03-26 | Massachusetts Institute Of Technology | Virus/nanowire encapsulation within polymer microgels for 2d and 3d devices for energy and electronics |
WO2009037680A2 (en) | 2007-09-20 | 2009-03-26 | Jean-Louis Viovy | Encapsulation microfluidic device |
US8408892B2 (en) * | 2007-10-05 | 2013-04-02 | Snu R&Db Foundation | Fluidic channel system and method for fabricating fine structure |
US20090098168A1 (en) * | 2007-10-08 | 2009-04-16 | The Regents Of The University Of California | Multiple-layer microbubble liposphere drug delivery vehicle and system |
US10725020B2 (en) | 2007-11-14 | 2020-07-28 | Curiox Biosystems Pte Ltd. | High throughput miniaturized assay system and methods |
WO2013114217A1 (en) | 2012-02-05 | 2013-08-08 | Curiox Biosystems Pte Ltd. | Array plates and methods for making and using same |
US9797010B2 (en) | 2007-12-21 | 2017-10-24 | President And Fellows Of Harvard College | Systems and methods for nucleic acid sequencing |
JP5462183B2 (en) | 2007-12-23 | 2014-04-02 | アドヴァンスト リキッド ロジック インコーポレイテッド | Droplet actuator configuration and method for directing droplet motion |
US8802027B2 (en) * | 2008-03-28 | 2014-08-12 | President And Fellows Of Harvard College | Surfaces, including microfluidic channels, with controlled wetting properties |
US8852952B2 (en) | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
WO2009139898A2 (en) | 2008-05-16 | 2009-11-19 | President And Fellows Of Harvard College | Valves and other flow control in fluidic systems including microfluidic systems |
JP4572973B2 (en) | 2008-06-16 | 2010-11-04 | ソニー株式会社 | Microchip and flow-feeding method in microchip |
JP2011525811A (en) * | 2008-06-27 | 2011-09-29 | マサチューセッツ インスティテュート オブ テクノロジー | Microfluidic droplets for metabolic engineering and other applications |
EP4047367A1 (en) | 2008-07-18 | 2022-08-24 | Bio-Rad Laboratories, Inc. | Method for detecting target analytes with droplet libraries |
JP2010038866A (en) * | 2008-08-08 | 2010-02-18 | Sony Corp | Microchip, particulate dispensing device, and feed flow method |
DE102008039117B3 (en) * | 2008-08-21 | 2010-05-20 | Institut für Bioprozess- und Analysenmesstechnik e.V. | Arrangement and method for generating, manipulating and analyzing compartments |
WO2010033200A2 (en) * | 2008-09-19 | 2010-03-25 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
US9598725B2 (en) * | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
US8663920B2 (en) | 2011-07-29 | 2014-03-04 | Bio-Rad Laboratories, Inc. | Library characterization by digital assay |
US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
US8633015B2 (en) | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US8748094B2 (en) | 2008-12-19 | 2014-06-10 | President And Fellows Of Harvard College | Particle-assisted nucleic acid sequencing |
EP2406003A2 (en) | 2009-03-13 | 2012-01-18 | President and Fellows of Harvard College | Scale-up of flow-focusing microfluidic devices |
WO2010104604A1 (en) | 2009-03-13 | 2010-09-16 | President And Fellows Of Harvard College | Method for the controlled creation of emulsions, including multiple emulsions |
EP3415235A1 (en) | 2009-03-23 | 2018-12-19 | Raindance Technologies Inc. | Manipulation of microfluidic droplets |
US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
AU2010229490B2 (en) | 2009-03-24 | 2015-02-12 | University Of Chicago | Slip chip device and methods |
EP2411134B1 (en) | 2009-03-25 | 2015-02-18 | Eastman Kodak Company | Droplet generation |
US8689981B2 (en) | 2009-04-10 | 2014-04-08 | President And Fellows Of Harvard College | Manipulation of particles in channels |
US9757698B2 (en) | 2009-06-26 | 2017-09-12 | President And Fellows Of Harvard College | Fluid injection |
US8926065B2 (en) | 2009-08-14 | 2015-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
EP2473263B1 (en) | 2009-09-02 | 2022-11-02 | President and Fellows of Harvard College | Multiple emulsions created using jetting and other techniques |
CA2767056C (en) | 2009-09-02 | 2018-12-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
US8746285B2 (en) * | 2009-09-04 | 2014-06-10 | Auburn University | Programmable fluidic droplet generation |
WO2011042564A1 (en) | 2009-10-09 | 2011-04-14 | Universite De Strasbourg | Labelled silica-based nanomaterial with enhanced properties and uses thereof |
TWI421339B (en) * | 2009-10-16 | 2014-01-01 | Academia Sinica | Method of fabricating three dimensional scaffolds and device thereof |
US8513014B2 (en) | 2009-10-20 | 2013-08-20 | Academia Sinica | Method for fabricating foam scaffolds to culture cells |
US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
EP2496700B1 (en) | 2009-11-04 | 2017-03-01 | The University Of British Columbia | Nucleic acid-containing lipid particles and related methods |
WO2011057197A2 (en) | 2009-11-06 | 2011-05-12 | Advanced Liquid Logic, Inc. | Integrated droplet actuator for gel electrophoresis and molecular analysis |
EP3085791A1 (en) | 2009-11-25 | 2016-10-26 | Gen9, Inc. | Methods and apparatuses for chip-based dna error reduction |
EP2516669B1 (en) | 2009-12-21 | 2016-10-12 | Advanced Liquid Logic, Inc. | Enzyme assays on a droplet actuator |
EP2517025B1 (en) | 2009-12-23 | 2019-11-27 | Bio-Rad Laboratories, Inc. | Methods for reducing the exchange of molecules between droplets |
US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
US8716467B2 (en) | 2010-03-03 | 2014-05-06 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
BR112012023441A2 (en) * | 2010-03-17 | 2016-05-24 | Basf Se | fusion emulsification |
EP2556170A4 (en) | 2010-03-25 | 2014-01-01 | Quantalife Inc | Droplet transport system for detection |
CA2767182C (en) | 2010-03-25 | 2020-03-24 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
CA2767113A1 (en) | 2010-03-25 | 2011-09-29 | Bio-Rad Laboratories, Inc. | Detection system for droplet-based assays |
USD673287S1 (en) | 2010-11-24 | 2012-12-25 | Sony Corporation | Micro flow channel chip |
USD869308S1 (en) | 2010-04-29 | 2019-12-10 | Sony Corporation | Micro flow channel chip |
USD673286S1 (en) | 2010-04-29 | 2012-12-25 | Sony Corporation | Micro flow channel chip |
CA2802049C (en) | 2010-06-07 | 2018-07-10 | Firefly Bioworks, Inc. | Scanning multifunctional particles |
US9499813B2 (en) | 2010-06-10 | 2016-11-22 | President And Fellows Of Harvard College | Systems and methods for amplification and phage display |
WO2012011877A2 (en) | 2010-07-23 | 2012-01-26 | Curiox Biosystems Pte Ltd | Apparatus and method for multiple reactions in small volumes |
JP2012024313A (en) * | 2010-07-23 | 2012-02-09 | Nitto Denko Corp | Device for forming droplets, and method for forming droplets |
US9695390B2 (en) | 2010-08-23 | 2017-07-04 | President And Fellows Of Harvard College | Acoustic waves in microfluidics |
EP3574990B1 (en) | 2010-11-01 | 2022-04-06 | Bio-Rad Laboratories, Inc. | System for forming emulsions |
WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
EP3000883B8 (en) | 2010-11-12 | 2018-02-28 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
US20120167410A1 (en) | 2010-12-21 | 2012-07-05 | Basf Se | Spray drying techniques |
KR20140063507A (en) | 2011-02-07 | 2014-05-27 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Systems and methods for splitting droplets |
EP2673382B1 (en) | 2011-02-11 | 2020-05-06 | Bio-Rad Laboratories, Inc. | Thermocycling device for nucleic acid amplification and methods of use |
CN103534360A (en) | 2011-03-18 | 2014-01-22 | 伯乐生命医学产品有限公司 | Multiplexed digital assays with combinatorial use of signals |
WO2012135327A1 (en) | 2011-03-31 | 2012-10-04 | Gnubio Inc. | Managing variation in spectroscopic intensity measurements through the use of a reference component |
US11130992B2 (en) | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
JP2014512826A (en) | 2011-04-25 | 2014-05-29 | バイオ−ラド ラボラトリーズ インコーポレイテッド | Methods and compositions for nucleic acid analysis |
WO2012154745A2 (en) | 2011-05-09 | 2012-11-15 | Advanced Liquid Logic, Inc. | Microfluidic feedback using impedance detection |
WO2012162296A2 (en) | 2011-05-23 | 2012-11-29 | President And Fellows Of Harvard College | Control of emulsions, including multiple emulsions |
EP3709018A1 (en) | 2011-06-02 | 2020-09-16 | Bio-Rad Laboratories, Inc. | Microfluidic apparatus for identifying components of a chemical reaction |
WO2013006661A2 (en) | 2011-07-06 | 2013-01-10 | President And Fellows Of Harvard College | Multiple emulsions and techniques for the formation of multiple emulsions |
AU2012279420A1 (en) | 2011-07-06 | 2014-01-30 | Advanced Liquid Logic Inc | Reagent storage on a droplet actuator |
WO2013009927A2 (en) | 2011-07-11 | 2013-01-17 | Advanced Liquid Logic, Inc. | Droplet actuators and techniques for droplet-based assays |
US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
WO2013016413A2 (en) | 2011-07-25 | 2013-01-31 | Advanced Liquid Logic Inc | Droplet actuator apparatus and system |
DK3594340T3 (en) | 2011-08-26 | 2021-09-20 | Gen9 Inc | COMPOSITIONS AND METHODS FOR COLLECTING WITH HIGH ACCURACY OF NUCLEIC ACIDS |
US20130064862A1 (en) | 2011-08-30 | 2013-03-14 | Basf Se | Systems and methods for shell encapsulation |
EP2760578B1 (en) * | 2011-09-28 | 2020-08-26 | President and Fellows of Harvard College | Systems and methods for droplet production and/or fluidic manipulation |
WO2013059922A1 (en) | 2011-10-25 | 2013-05-02 | The University Of British Columbia | Limit size lipid nanoparticles and related methods |
CN102500489A (en) * | 2011-11-06 | 2012-06-20 | 中国科学技术大学 | Spray gun spray nozzle capable of realizing minuteness atomization |
WO2013078216A1 (en) | 2011-11-21 | 2013-05-30 | Advanced Liquid Logic Inc | Glucose-6-phosphate dehydrogenase assays |
US8771611B2 (en) | 2011-11-28 | 2014-07-08 | Auburn University | System and methods of log-scale concentration gradients |
KR20140122751A (en) | 2012-02-08 | 2014-10-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Droplet formation using fluid breakup |
WO2013120089A1 (en) | 2012-02-10 | 2013-08-15 | Raindance Technologies, Inc. | Molecular diagnostic screening assay |
US9176031B2 (en) | 2012-02-24 | 2015-11-03 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
LT3305918T (en) | 2012-03-05 | 2020-09-25 | President And Fellows Of Harvard College | Methods for epigenetic sequencing |
JP6115930B2 (en) * | 2012-03-12 | 2017-04-19 | 国立研究開発法人産業技術総合研究所 | Multi-stage split channel mixer |
US10080997B2 (en) * | 2012-03-16 | 2018-09-25 | Versitech Limited | System and method for generation of emulsions with low interfacial tension and measuring frequency vibrations in the system |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
EP2827979A1 (en) * | 2012-03-22 | 2015-01-28 | Universiteit Twente | Apparatus and method for mass producing a monodisperse microbubble agent |
US8939551B2 (en) | 2012-03-28 | 2015-01-27 | Eastman Kodak Company | Digital drop patterning device and method |
US8936354B2 (en) | 2012-03-28 | 2015-01-20 | Eastman Kodak Company | Digital drop patterning device and method |
US8936353B2 (en) | 2012-03-28 | 2015-01-20 | Eastman Kodak Company | Digital drop patterning device and method |
US8602535B2 (en) | 2012-03-28 | 2013-12-10 | Eastman Kodak Company | Digital drop patterning device and method |
US20150177115A1 (en) | 2012-04-06 | 2015-06-25 | Slingshot Biosciences | Hydrogel particles with tunable optical properties |
WO2013155531A2 (en) | 2012-04-13 | 2013-10-17 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
CA2870999A1 (en) | 2012-04-20 | 2013-10-24 | SlipChip, LLC | Fluidic devices and systems for sample preparation or autonomous analysis |
WO2013159116A1 (en) | 2012-04-20 | 2013-10-24 | University Of Chicago | Fluidic devices for biospecimen preservation |
EP3543350B1 (en) | 2012-04-24 | 2021-11-10 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
WO2013163246A2 (en) | 2012-04-25 | 2013-10-31 | President And Fellows Of Harvard College | Polymerization reactions within microfluidic devices |
WO2013165748A1 (en) | 2012-04-30 | 2013-11-07 | Raindance Technologies, Inc | Digital analyte analysis |
IL236303B (en) | 2012-06-25 | 2022-07-01 | Gen9 Inc | Methods for nucleic acid assembly and high throughput sequencing |
CA2877950C (en) | 2012-06-27 | 2021-06-22 | Advanced Liquid Logic Inc. | Techniques and droplet actuator designs for reducing bubble formation |
EP2882872B1 (en) | 2012-08-13 | 2021-10-06 | The Regents of The University of California | Methods and systems for detecting biological components |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9328376B2 (en) * | 2012-09-05 | 2016-05-03 | Bio-Rad Laboratories, Inc. | Systems and methods for stabilizing droplets |
EP2895591A4 (en) | 2012-09-12 | 2016-10-12 | Gnubio Inc | Integrated microfluidic system, method and kit for performing assays |
JP2016502632A (en) | 2012-09-21 | 2016-01-28 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Systems and methods for spray drying for microfluidics and other systems |
WO2014085801A1 (en) | 2012-11-30 | 2014-06-05 | The Broad Institute, Inc. | Cryo-treatment in a microfluidic device |
EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP2954065B1 (en) | 2013-02-08 | 2021-07-28 | 10X Genomics, Inc. | Partitioning and processing of analytes and other species |
CN108212237B (en) * | 2013-03-06 | 2020-12-08 | 哈佛学院院长及董事 | Apparatus and method for forming relatively monodisperse droplets |
WO2014153071A1 (en) | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Methods for quantitating dna using digital multiple displacement amplification |
CA2906076A1 (en) | 2013-03-15 | 2014-09-18 | Abvitro, Inc. | Single cell bar-coding for antibody discovery |
CN105143456A (en) | 2013-03-15 | 2015-12-09 | 不列颠哥伦比亚大学 | Lipid nanoparticles for transfection and related methods |
GB201306445D0 (en) | 2013-04-09 | 2013-05-22 | Base4 Innovation Ltd | Single nucleotide detection method |
GB201306444D0 (en) | 2013-04-09 | 2013-05-22 | Base4 Innovation Ltd | Single nucleotide detection method |
EP2986762B1 (en) | 2013-04-19 | 2019-11-06 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
CN103285947A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Droplet micro-fluidic chip and operation method thereof |
CN103285946A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Biochip and control method thereof |
KR20140144408A (en) * | 2013-06-11 | 2014-12-19 | 삼성전기주식회사 | Droplet forming device and method for forming droplet using the same |
US11141730B2 (en) | 2013-06-14 | 2021-10-12 | President And Fellows Of Harvard College | Coalescence of droplets |
US9557318B2 (en) | 2013-07-09 | 2017-01-31 | Curiox Biosystems Pte Ltd. | Array plates for washing samples |
US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
US9233859B2 (en) | 2013-09-30 | 2016-01-12 | Uchicago Argonne, Llc. | Microfluidic process monitor for industrial solvent extraction system |
US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
WO2015069634A1 (en) | 2013-11-08 | 2015-05-14 | President And Fellows Of Harvard College | Microparticles, methods for their preparation and use |
US10801070B2 (en) | 2013-11-25 | 2020-10-13 | The Broad Institute, Inc. | Compositions and methods for diagnosing, evaluating and treating cancer |
US11725237B2 (en) | 2013-12-05 | 2023-08-15 | The Broad Institute Inc. | Polymorphic gene typing and somatic change detection using sequencing data |
US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
CN106456724A (en) | 2013-12-20 | 2017-02-22 | 博德研究所 | Combination therapy with neoantigen vaccine |
US11193176B2 (en) | 2013-12-31 | 2021-12-07 | Bio-Rad Laboratories, Inc. | Method for detecting and quantifying latent retroviral RNA species |
WO2015130968A2 (en) | 2014-02-27 | 2015-09-03 | The Broad Institute Inc. | T cell balance gene expression, compositions of matters and methods of use thereof |
JP6031711B2 (en) * | 2014-03-11 | 2016-11-24 | 凸版印刷株式会社 | Droplet manufacturing device, droplet manufacturing method, liposome manufacturing method, fixture, and droplet manufacturing kit |
AU2015243445B2 (en) | 2014-04-10 | 2020-05-28 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
WO2015160919A1 (en) | 2014-04-16 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for producing droplet emulsions with relatively thin shells |
US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
EP3456846B1 (en) | 2014-04-21 | 2022-06-22 | President and Fellows of Harvard College | Systems and methods for barcoding nucleic acid |
CA2948976A1 (en) | 2014-05-14 | 2015-11-19 | University Of Limerick | Method for testing compounds on living cells |
CN106536709A (en) | 2014-06-16 | 2017-03-22 | 基纽拜奥股份有限公司 | Size alternating injection into drops to facilitate sorting |
CN106795553B (en) | 2014-06-26 | 2021-06-04 | 10X基因组学有限公司 | Methods of analyzing nucleic acids from individual cells or cell populations |
US10258987B2 (en) | 2014-06-26 | 2019-04-16 | President And Fellows Of Harvard College | Fluid infection using acoustic waves |
CN106575322B (en) | 2014-06-26 | 2019-06-18 | 10X基因组学有限公司 | The method and system of nucleic acid sequence assembly |
US10697007B2 (en) | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
US9999895B2 (en) | 2014-08-06 | 2018-06-19 | S. C. Johnson & Son, Inc. | Spray inserts |
WO2016040476A1 (en) | 2014-09-09 | 2016-03-17 | The Broad Institute, Inc. | A droplet-based method and apparatus for composite single-cell nucleic acid analysis |
EP3950944A1 (en) | 2014-09-15 | 2022-02-09 | AbVitro LLC | High-throughput nucleotide library sequencing |
EP3209419A4 (en) | 2014-10-22 | 2018-10-03 | The Regents of The University of California | High definition microdroplet printer |
KR20170073667A (en) | 2014-10-29 | 2017-06-28 | 10엑스 제노믹스, 인크. | Methods and compositions for targeted nucleic acid sequencing |
US10000799B2 (en) | 2014-11-04 | 2018-06-19 | Boreal Genomics, Inc. | Methods of sequencing with linked fragments |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
JP6742311B2 (en) | 2014-11-14 | 2020-08-19 | アテナ ダイアグナスティクス,インコーポレイテッド | Method for detecting silent carrier genotype |
WO2016085742A1 (en) | 2014-11-24 | 2016-06-02 | The Procter & Gamble Company | Methods for encapsulation of actives within droplets and other compartments |
EP3234193B1 (en) | 2014-12-19 | 2020-07-15 | Massachusetts Institute of Technology | Molecular biomarkers for cancer immunotherapy |
WO2016100977A1 (en) | 2014-12-19 | 2016-06-23 | The Broad Institute Inc. | Methods for profiling the t-cel- receptor repertoire |
CN112126675B (en) | 2015-01-12 | 2022-09-09 | 10X基因组学有限公司 | Method and system for preparing nucleic acid sequencing library and library prepared by using same |
CN107209814B (en) | 2015-01-13 | 2021-10-15 | 10X基因组学有限公司 | System and method for visualizing structural variation and phase information |
NL2014178B1 (en) * | 2015-01-22 | 2017-01-05 | Tide Microfluidics B V | System and method for controlled manufacturing of mono-disperse microbubbles. |
EP3247675A4 (en) | 2015-01-23 | 2018-07-04 | Neofluidics LLC | A microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors |
US20160238506A1 (en) * | 2015-02-02 | 2016-08-18 | Derek Oberreit | Ice nucleii counter technology |
AU2016215304B2 (en) | 2015-02-04 | 2022-01-27 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
US20160358722A1 (en) * | 2015-02-05 | 2016-12-08 | Ramasamy Lakshmanan | Intelligent wireless and wired control of devices |
CN114923837A (en) | 2015-02-09 | 2022-08-19 | 弹弓生物科学公司 | Hydrogel particles with tunable optical properties and methods of using the same |
CA2975529A1 (en) | 2015-02-09 | 2016-08-18 | 10X Genomics, Inc. | Systems and methods for determining structural variation and phasing using variant call data |
WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
EP3262188B1 (en) | 2015-02-24 | 2021-05-05 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
EP3262193A2 (en) | 2015-02-26 | 2018-01-03 | The Broad Institute Inc. | T cell balance gene expression, compositions of matters and methods of use thereof |
WO2016145409A1 (en) | 2015-03-11 | 2016-09-15 | The Broad Institute, Inc. | Genotype and phenotype coupling |
CN107614096A (en) | 2015-03-13 | 2018-01-19 | 哈佛学院院长及董事 | Use amplification assay cell |
CN107614684A (en) | 2015-04-17 | 2018-01-19 | 哈佛学院院长及董事 | Bar coding system and method for gene sequencing and other application |
CR20230191A (en) | 2015-05-20 | 2023-07-06 | Dana Farber Cancer Inst Inc | SHARED NEOANTIGENS (Div. exp 2017-584) |
CN107405633A (en) * | 2015-05-22 | 2017-11-28 | 香港科技大学 | Droplet generator based on high-aspect-ratio inductive formation drop |
US10545139B2 (en) | 2015-06-16 | 2020-01-28 | Curiox Biosystems Pte Ltd. | Methods and devices for performing biological assays using magnetic components |
WO2016205728A1 (en) | 2015-06-17 | 2016-12-22 | Massachusetts Institute Of Technology | Crispr mediated recording of cellular events |
EP3314046B1 (en) | 2015-06-25 | 2019-04-24 | Hooke Bio Limited | Mechanical device for generating combinatorial library |
US11690309B2 (en) | 2015-07-23 | 2023-07-04 | Zito Jr Arthur J | Responsive dispersion from compartment in aqueous solution |
US10809250B2 (en) | 2015-08-25 | 2020-10-20 | Bio-Rad Laboratories, Inc. | Digital immunoassay |
AU2016311341B2 (en) | 2015-08-27 | 2021-01-28 | President And Fellows Of Harvard College | Acoustic wave sorting |
US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
EP3353325B1 (en) | 2015-09-24 | 2024-03-20 | AbVitro LLC | Single amplicon activated exclusion pcr |
CA2999888A1 (en) | 2015-09-24 | 2017-03-30 | Abvitro Llc | Affinity-oligonucleotide conjugates and uses thereof |
US10928392B2 (en) | 2015-09-25 | 2021-02-23 | Abvitro Llc | High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences |
CN108289797B (en) | 2015-10-13 | 2022-01-28 | 哈佛学院院长及董事 | Systems and methods for making and using gel microspheres |
SG10202107069UA (en) | 2015-10-27 | 2021-07-29 | Berkeley Lights Inc | Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface |
US11092607B2 (en) | 2015-10-28 | 2021-08-17 | The Board Institute, Inc. | Multiplex analysis of single cell constituents |
WO2017075297A1 (en) | 2015-10-28 | 2017-05-04 | The Broad Institute Inc. | High-throughput dynamic reagent delivery system |
WO2017075294A1 (en) | 2015-10-28 | 2017-05-04 | The Board Institute Inc. | Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction |
CN105435869B (en) * | 2015-11-06 | 2017-05-10 | 常州工学院 | Apparatus and method for micro-droplet split in microchannel |
US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
EP3377627B1 (en) | 2015-11-20 | 2020-11-04 | Bio-Rad Laboratories, Inc. | Sparse identity spaces in droplet sequencing |
US10774370B2 (en) | 2015-12-04 | 2020-09-15 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
US20190144936A1 (en) | 2016-01-15 | 2019-05-16 | Massachusetts Institute Of Technology | Semi-permeable arrays for analyzing biological systems and methods of using same |
EP3411710A1 (en) | 2016-02-05 | 2018-12-12 | The Broad Institute Inc. | Multi-stage, multiplexed target isolation and processing from heterogeneous populations |
SG11201806757XA (en) | 2016-02-11 | 2018-09-27 | 10X Genomics Inc | Systems, methods, and media for de novo assembly of whole genome sequence data |
US20190144942A1 (en) | 2016-02-22 | 2019-05-16 | Massachusetts Institute Of Technology | Methods for identifying and modulating immune phenotypes |
WO2017161325A1 (en) | 2016-03-17 | 2017-09-21 | Massachusetts Institute Of Technology | Methods for identifying and modulating co-occurant cellular phenotypes |
EP3436607B1 (en) | 2016-03-28 | 2023-06-14 | Ncan Genomics, Inc. | Linked duplex target capture |
US10961573B2 (en) | 2016-03-28 | 2021-03-30 | Boreal Genomics, Inc. | Linked duplex target capture |
JP2019514002A (en) | 2016-04-15 | 2019-05-30 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Systems and methods for the collection of droplets and / or other entities |
CN105712319B (en) * | 2016-04-29 | 2018-01-23 | 清华大学 | The preparation facilities of macroscopical aggregation of micro Nano material |
JP2019523109A (en) | 2016-05-03 | 2019-08-22 | ニューマ・リスパイラトリー・インコーポレイテッド | Systems and methods for lung health management |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
EP3686287A1 (en) | 2016-06-28 | 2020-07-29 | Hifibio | Method for transcriptome analysis of single cells |
US20220008918A1 (en) | 2016-07-08 | 2022-01-13 | California Institute Of Technology | Methods and devices for performing flow-through capture of low-concentration analytes |
EP3481540B1 (en) | 2016-07-08 | 2022-12-21 | President and Fellows of Harvard College | Formation of colloids or gels within droplets |
US11142791B2 (en) | 2016-08-10 | 2021-10-12 | The Regents Of The University Of California | Combined multiple-displacement amplification and PCR in an emulsion microdroplet |
US10654040B2 (en) | 2016-08-18 | 2020-05-19 | Northeastern University | Platform for liquid droplet formation and isolation |
JP6929354B2 (en) | 2016-09-24 | 2021-09-01 | アブビトロ, エルエルシー | Affinity-oligonucleotide conjugates and their use |
US11268137B2 (en) | 2016-12-09 | 2022-03-08 | Boreal Genomics, Inc. | Linked ligation |
CA3047328A1 (en) | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3574116A1 (en) | 2017-01-24 | 2019-12-04 | The Broad Institute, Inc. | Compositions and methods for detecting a mutant variant of a polynucleotide |
WO2018140966A1 (en) | 2017-01-30 | 2018-08-02 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
EP3375889B1 (en) | 2017-03-17 | 2019-12-11 | HiFiBiO SAS | Single cell analysis |
US20200115753A1 (en) | 2017-03-17 | 2020-04-16 | Massachusetts Institute Of Technology | Methods for identifying and modulating co-occurant cellular phenotypes |
KR102446247B1 (en) | 2017-04-05 | 2022-09-21 | 큐리옥스 바이오시스템즈 피티이 엘티디. | Methods, devices and apparatus for cleaning samples on array plates |
MX2019012398A (en) | 2017-04-18 | 2020-09-25 | Broad Inst Inc | Compositions for detecting secretion and methods of use. |
EP3615220A4 (en) | 2017-04-28 | 2020-12-30 | Neofluidics, LLC | Fluidic devices with reaction wells and uses thereof |
US11072816B2 (en) | 2017-05-03 | 2021-07-27 | The Broad Institute, Inc. | Single-cell proteomic assay using aptamers |
US11920192B2 (en) | 2017-05-15 | 2024-03-05 | Lightcast Discovery Ltd | Single nucleotide detection method and associated probes |
EP4215616A1 (en) | 2017-05-18 | 2023-07-26 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
EP3625715A4 (en) | 2017-05-19 | 2021-03-17 | 10X Genomics, Inc. | Systems and methods for analyzing datasets |
EP4223335A3 (en) | 2017-05-19 | 2023-11-08 | Pneuma Respiratory, Inc. | Dry powder delivery device and methods of use |
WO2018217831A1 (en) | 2017-05-22 | 2018-11-29 | Arizona Board Of Regents On Behalf Of Arizona State University | Metal electrode based 3d printed device for tuning microfluidic droplet generation frequency and synchronizing phase for serial femtosecond crystallography |
WO2018218222A1 (en) | 2017-05-26 | 2018-11-29 | Goldfless Stephen Jacob | High-throughput polynucleotide library sequencing and transcriptome analysis |
US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
EP4230746A3 (en) | 2017-05-26 | 2023-11-01 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
EP3665262A4 (en) | 2017-08-09 | 2021-09-01 | Neofluidics, LLC | Devices and methods for bioassay |
US10357771B2 (en) | 2017-08-22 | 2019-07-23 | 10X Genomics, Inc. | Method of producing emulsions |
EP3679370A1 (en) | 2017-09-07 | 2020-07-15 | Juno Therapeutics, Inc. | Methods of identifying cellular attributes related to outcomes associated with cell therapy |
US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
EP3691728A4 (en) | 2017-10-04 | 2021-06-30 | Pneuma Respiratory, Inc. | Electronic breath actuated in-line droplet delivery device and methods of use |
EP3697481A4 (en) | 2017-10-17 | 2021-07-14 | Pneuma Respiratory, Inc. | Nasal drug delivery apparatus and methods of use |
EP3697537A4 (en) | 2017-10-18 | 2021-10-20 | Group K Diagnostics, Inc. | Single-layer microfluidic device and methods of manufacture and use thereof |
US10501739B2 (en) | 2017-10-18 | 2019-12-10 | Mission Bio, Inc. | Method, systems and apparatus for single cell analysis |
WO2019084055A1 (en) | 2017-10-23 | 2019-05-02 | Massachusetts Institute Of Technology | Calling genetic variation from single-cell transcriptomes |
WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | Microfluidic channel networks for partitioning |
WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | Methods and systems for nuclecic acid preparation and chromatin analysis |
EP4241882A3 (en) | 2017-10-27 | 2023-12-06 | 10X Genomics, Inc. | Methods for sample preparation and analysis |
EP3706843A4 (en) | 2017-11-08 | 2021-08-25 | Pneuma Respiratory, Inc. | Electronic breath actuated in-line droplet delivery device with small volume ampoule and methods of use |
US11305279B2 (en) | 2017-11-10 | 2022-04-19 | Neofluidics, Llc | Integrated fluidic circuit and device for droplet manipulation and methods thereof |
EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
CN107941659B (en) * | 2017-11-20 | 2020-05-19 | 武汉科技大学 | Seepage velocity measuring device in fracture water freezing process |
WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
WO2019113506A1 (en) | 2017-12-07 | 2019-06-13 | The Broad Institute, Inc. | Methods and compositions for multiplexing single cell and single nuclei sequencing |
US11173487B2 (en) | 2017-12-19 | 2021-11-16 | Arizona Board Of Regents On Behalf Of Arizona State University | Deterministic ratchet for sub-micrometer bioparticle separation |
BR112020013065A2 (en) | 2017-12-28 | 2020-12-01 | Ethicon Llc | surgical instruments comprising button circuits |
WO2019157529A1 (en) | 2018-02-12 | 2019-08-15 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
US11841371B2 (en) | 2018-03-13 | 2023-12-12 | The Broad Institute, Inc. | Proteomics and spatial patterning using antenna networks |
CN112262218A (en) | 2018-04-06 | 2021-01-22 | 10X基因组学有限公司 | System and method for quality control in single cell processing |
US11414701B2 (en) | 2018-05-24 | 2022-08-16 | The Broad Institute, Inc. | Multimodal readouts for quantifying and sequencing nucleic acids in single cells |
US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
EP3807014A4 (en) | 2018-06-14 | 2022-03-02 | Regents of the University of Minnesota | Counterflow mixer and atomizer |
US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
US20220001122A1 (en) * | 2018-10-01 | 2022-01-06 | Pneuma Respiratory, Inc. | Delivery of low surface tension compositions to the pulmonary system via electronic breath actuated droplet delivery device |
US20220411783A1 (en) | 2018-10-12 | 2022-12-29 | The Broad Institute, Inc. | Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues |
GB201817321D0 (en) | 2018-10-24 | 2018-12-05 | Nanna Therapeutics Ltd | Microbeads for tagless encoded chemical library screening |
USD879999S1 (en) | 2018-11-02 | 2020-03-31 | Group K Diagnostics, Inc. | Microfluidic device |
CA3119972A1 (en) | 2018-11-14 | 2020-05-22 | Cameron MYHRVOLD | Crispr system based droplet diagnostic systems and methods |
CN113302312A (en) | 2018-11-14 | 2021-08-24 | 博德研究所 | Multiplexing of highly evolved virus variants using the SHERLock detection method |
US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
WO2020123657A2 (en) | 2018-12-11 | 2020-06-18 | 10X Genomics, Inc. | Methods and devices for detecting and sorting droplets or particles |
WO2020124050A1 (en) | 2018-12-13 | 2020-06-18 | The Broad Institute, Inc. | Tiled assays using crispr-cas based detection |
US20220062394A1 (en) | 2018-12-17 | 2022-03-03 | The Broad Institute, Inc. | Methods for identifying neoantigens |
EP3670667A1 (en) | 2018-12-19 | 2020-06-24 | Paris Sciences et Lettres - Quartier Latin | Identification of cognate pairs of ligands and receptors |
WO2020139844A1 (en) | 2018-12-24 | 2020-07-02 | 10X Genomics, Inc. | Devices, systems, and methods for controlling liquid flow |
WO2020141464A1 (en) | 2019-01-03 | 2020-07-09 | Boreal Genomics, Inc. | Linked target capture |
US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
US20220119871A1 (en) | 2019-01-28 | 2022-04-21 | The Broad Institute, Inc. | In-situ spatial transcriptomics |
US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
SG11202108788TA (en) | 2019-02-12 | 2021-09-29 | 10X Genomics Inc | Methods for processing nucleic acid molecules |
EP3698871A1 (en) | 2019-02-19 | 2020-08-26 | Gottfried Wilhelm Leibniz Universität Hannover | Laser based sorting of droplets in microfluidic streams |
US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
WO2020176449A1 (en) | 2019-02-26 | 2020-09-03 | President And Fellows Of Harvard College | Systems and methods for high throughput selection |
EP3930900A1 (en) | 2019-02-28 | 2022-01-05 | 10X Genomics, Inc. | Devices, systems, and methods for increasing droplet formation efficiency |
US11920183B2 (en) | 2019-03-11 | 2024-03-05 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
US11624718B2 (en) | 2019-05-14 | 2023-04-11 | Arizona Board Of Regents On Behalf Of Arizona State University | Single piece droplet generation and injection device for serial crystallography |
US11318487B2 (en) * | 2019-05-14 | 2022-05-03 | Arizona Board Of Regents On Behalf Of Arizona State University | Co-flow injection for serial crystallography |
EP3990071A4 (en) * | 2019-06-27 | 2023-07-19 | Pneuma Respiratory, Inc. | Delivery of small droplets to the respiratory system via electronic breath actuated droplet delivery device |
EP3763439A1 (en) * | 2019-07-12 | 2021-01-13 | Curiosity Diagnostics sp. z o.o | Microfluidic chip and valve, production process and uses |
CN110404701A (en) * | 2019-07-16 | 2019-11-05 | 清华大学 | A kind of control method and device of jet crushing |
US11701658B2 (en) | 2019-08-09 | 2023-07-18 | President And Fellows Of Harvard College | Systems and methods for microfluidic particle selection, encapsulation, and injection using surface acoustic waves |
US11919002B2 (en) | 2019-08-20 | 2024-03-05 | 10X Genomics, Inc. | Devices and methods for generating and recovering droplets |
GB201914537D0 (en) | 2019-10-08 | 2019-11-20 | Univ Southampton | Transcript analysis |
JP2022552194A (en) | 2019-10-10 | 2022-12-15 | 1859,インク. | Methods and systems for microfluidic screening |
CN110787846B (en) * | 2019-11-05 | 2021-04-16 | 华中科技大学 | One-step double-layer micro-droplet generation device and method |
CA3165821A1 (en) | 2020-01-24 | 2021-07-29 | Jeffrey Kim | Compositions and methods for cell-like calibration particles |
GB202005615D0 (en) | 2020-04-17 | 2020-06-03 | Sphere Fluidics Ltd | Droplet spacing |
KR20230029612A (en) | 2020-05-04 | 2023-03-03 | 슬링샷 바이오사이언시즈 인코포레이티드 | Compositions and methods for passive optical barcoding for multiple assays |
US11701668B1 (en) | 2020-05-08 | 2023-07-18 | 10X Genomics, Inc. | Methods and devices for magnetic separation |
US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
US11594340B2 (en) | 2020-05-13 | 2023-02-28 | Battelle Savannah River Alliance, Llc | Manufacture of particulate reference materials |
US11946038B1 (en) | 2020-05-29 | 2024-04-02 | 10X Genomics, Inc. | Methods and systems including flow and magnetic modules |
CA3183029A1 (en) | 2020-06-24 | 2021-12-30 | Hifibio (Hk) Limited | Methods for identification of cognate pairs of ligands and receptors |
EP3950772A1 (en) | 2020-08-05 | 2022-02-09 | Emulseo SAS | Novel fluorosurfactants and uses thereof in microfluidics |
WO2022051522A1 (en) | 2020-09-02 | 2022-03-10 | 10X Genomics, Inc. | Flow focusing devices, systems, and methods for high throughput droplet formation |
CN116171200A (en) | 2020-09-02 | 2023-05-26 | 10X基因组学有限公司 | Apparatus, system and method for high throughput drop formation |
US11485632B2 (en) | 2020-10-09 | 2022-11-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Modular 3-D printed devices for sample delivery and method |
WO2022146770A1 (en) | 2020-12-28 | 2022-07-07 | Neofluidics Llc | A microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors and method of operation |
WO2022182682A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
WO2022182865A1 (en) | 2021-02-24 | 2022-09-01 | 10X Genomics, Inc. | Method for concentrating droplets in an emulsion |
EP4313412A1 (en) | 2021-03-26 | 2024-02-07 | 10X Genomics, Inc. | Devices, methods, and systems for improved droplet recovery |
WO2022232050A1 (en) | 2021-04-26 | 2022-11-03 | The Broad Institute, Inc. | Compositions and methods for characterizing polynucleotide sequence alterations |
WO2022271848A1 (en) | 2021-06-22 | 2022-12-29 | Pneuma Respiratory, Inc. | Droplet delivery device with push ejection |
WO2023004068A2 (en) | 2021-07-21 | 2023-01-26 | 10X Genomics, Inc. | Methods, devices, and kits for purifying and lysing biological particles |
WO2023021295A2 (en) | 2021-08-18 | 2023-02-23 | Nuclera Nucleics Ltd | Methods and compositions for improved biomolecule assays on digital microfluidic devices |
WO2023076629A1 (en) | 2021-10-29 | 2023-05-04 | Slingshot Biosciences, Inc. | Hydrogel particles as feeder cells and as synthetic antigen presenting cells |
WO2023099667A1 (en) | 2021-12-01 | 2023-06-08 | Vilnius University | Methods for processing and barcoding nucleic acids |
WO2023168423A1 (en) | 2022-03-04 | 2023-09-07 | 10X Genomics, Inc. | Droplet forming devices and methods having fluoropolymer silane coating agents |
WO2023215886A1 (en) | 2022-05-05 | 2023-11-09 | Slingshot Biosciences, Inc. | Engineered particles as red blood cell mimics and compositions containing same for hematology |
US20240026339A1 (en) | 2022-07-10 | 2024-01-25 | Vilnius University | Composition and the use of cell lysis reagents |
WO2024039763A2 (en) | 2022-08-18 | 2024-02-22 | 10X Genomics, Inc. | Droplet forming devices and methods having flourous diol additives |
WO2024058036A1 (en) * | 2022-09-14 | 2024-03-21 | 国立大学法人 鹿児島大学 | Nozzle for bubble formation, bubble formation device, bubble formation method, and method for producing nozzle for bubble formation |
Family Cites Families (163)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2692800A (en) * | 1951-10-08 | 1954-10-26 | Gen Electric | Nozzle flow control |
US3980541A (en) * | 1967-06-05 | 1976-09-14 | Aine Harry E | Electrode structures for electric treatment of fluids and filters using same |
US3816331A (en) * | 1972-07-05 | 1974-06-11 | Ncr | Continuous encapsulation and device therefor |
CH563807A5 (en) * | 1973-02-14 | 1975-07-15 | Battelle Memorial Institute | Fine granules and microcapsules mfrd. from liquid droplets - partic. of high viscosity requiring forced sepn. of droplets |
CH564966A5 (en) * | 1974-02-25 | 1975-08-15 | Sauter Fr Ag Fabrik Elektrisch | |
US4059552A (en) | 1974-06-21 | 1977-11-22 | The Dow Chemical Company | Cross-linked water-swellable polymer particles |
US3982541A (en) | 1974-07-29 | 1976-09-28 | Esperance Jr Francis A L | Eye surgical instrument |
JPS5372016A (en) * | 1976-12-08 | 1978-06-27 | Toyo Tire & Rubber Co Ltd | Apparatus for preparation and supply of heavy oil w/o emulsion fuel |
US4279345A (en) * | 1979-08-03 | 1981-07-21 | Allred John C | High speed particle sorter using a field emission electrode |
GB2097692B (en) | 1981-01-10 | 1985-05-22 | Shaw Stewart P D | Combining chemical reagents |
JPS6057907B2 (en) * | 1981-06-18 | 1985-12-17 | 工業技術院長 | Liquid mixing and atomization method |
DE3230289A1 (en) * | 1982-08-14 | 1984-02-16 | Bayer Ag, 5090 Leverkusen | PRODUCTION OF PHARMACEUTICAL OR COSMETIC DISPERSIONS |
US4853336A (en) | 1982-11-15 | 1989-08-01 | Technicon Instruments Corporation | Single channel continuous flow system |
US4618476A (en) * | 1984-02-10 | 1986-10-21 | Eastman Kodak Company | Capillary transport device having speed and meniscus control means |
CA1235367A (en) * | 1984-04-05 | 1988-04-19 | Gary J. Green | Method and apparatus for producing uniform liquid droplets |
US4865444A (en) * | 1984-04-05 | 1989-09-12 | Mobil Oil Corporation | Apparatus and method for determining luminosity of hydrocarbon fuels |
GB8604328D0 (en) * | 1986-02-21 | 1986-03-26 | Ici Plc | Producing spray of droplets of liquid |
US4916070A (en) | 1986-04-14 | 1990-04-10 | The General Hospital Corporation | Fibrin-specific antibodies and method of screening for the antibodies |
US5204112A (en) * | 1986-06-16 | 1993-04-20 | The Liposome Company, Inc. | Induction of asymmetry in vesicles |
US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
US4931225A (en) * | 1987-12-30 | 1990-06-05 | Union Carbide Industrial Gases Technology Corporation | Method and apparatus for dispersing a gas into a liquid |
US5093602A (en) * | 1989-11-17 | 1992-03-03 | Charged Injection Corporation | Methods and apparatus for dispersing a fluent material utilizing an electron beam |
JP3176607B2 (en) | 1990-02-07 | 2001-06-18 | 群馬大学長 | Method for forming uniform droplets |
US6149789A (en) * | 1990-10-31 | 2000-11-21 | Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Process for manipulating microscopic, dielectric particles and a device therefor |
DE4127405C2 (en) | 1991-08-19 | 1996-02-29 | Fraunhofer Ges Forschung | Process for the separation of mixtures of microscopic dielectric particles suspended in a liquid or a gel and device for carrying out the process |
SE500071C2 (en) * | 1992-06-25 | 1994-04-11 | Vattenfall Utveckling Ab | Device for mixing two fluids, in particular liquids of different temperature |
DE4308839C2 (en) * | 1993-03-19 | 1997-04-30 | Jordanow & Co Gmbh | Device for mixing flow media |
US5512131A (en) | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
BR9502777A (en) * | 1994-06-13 | 1996-04-23 | Praxair Technology Inc | Liquid fuel atomization equipment and process |
US5935331A (en) * | 1994-09-09 | 1999-08-10 | Matsushita Electric Industrial Co., Ltd. | Apparatus and method for forming films |
US5762775A (en) * | 1994-09-21 | 1998-06-09 | Lockheed Martin Energy Systems, Inc. | Method for electrically producing dispersions of a nonconductive fluid in a conductive medium |
JPH08153669A (en) | 1994-11-30 | 1996-06-11 | Hitachi Ltd | Thin film forming method and formation device |
WO1996029629A2 (en) | 1995-03-01 | 1996-09-26 | President And Fellows Of Harvard College | Microcontact printing on surfaces and derivative articles |
JP3232525B2 (en) | 1995-08-22 | 2001-11-26 | 信越化学工業株式会社 | Water repellent agent |
US6130098A (en) * | 1995-09-15 | 2000-10-10 | The Regents Of The University Of Michigan | Moving microdroplets |
US5851769A (en) | 1995-09-27 | 1998-12-22 | The Regents Of The University Of California | Quantitative DNA fiber mapping |
JP3759986B2 (en) * | 1995-12-07 | 2006-03-29 | フロイント産業株式会社 | Seamless capsule and manufacturing method thereof |
US5681600A (en) * | 1995-12-18 | 1997-10-28 | Abbott Laboratories | Stabilization of liquid nutritional products and method of making |
US5868322A (en) | 1996-01-31 | 1999-02-09 | Hewlett-Packard Company | Apparatus for forming liquid droplets having a mechanically fixed inner microtube |
US6355198B1 (en) | 1996-03-15 | 2002-03-12 | President And Fellows Of Harvard College | Method of forming articles including waveguides via capillary micromolding and microtransfer molding |
US5942443A (en) * | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
US6197835B1 (en) * | 1996-05-13 | 2001-03-06 | Universidad De Sevilla | Device and method for creating spherical particles of uniform size |
US6187214B1 (en) | 1996-05-13 | 2001-02-13 | Universidad De Seville | Method and device for production of components for microfabrication |
US6299145B1 (en) * | 1996-05-13 | 2001-10-09 | Universidad De Sevilla | Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber |
US6196525B1 (en) * | 1996-05-13 | 2001-03-06 | Universidad De Sevilla | Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber |
ES2140998B1 (en) * | 1996-05-13 | 2000-10-16 | Univ Sevilla | LIQUID ATOMIZATION PROCEDURE. |
US6248378B1 (en) | 1998-12-16 | 2001-06-19 | Universidad De Sevilla | Enhanced food products |
US6405936B1 (en) * | 1996-05-13 | 2002-06-18 | Universidad De Sevilla | Stabilized capillary microjet and devices and methods for producing same |
US6386463B1 (en) * | 1996-05-13 | 2002-05-14 | Universidad De Sevilla | Fuel injection nozzle and method of use |
US6189803B1 (en) * | 1996-05-13 | 2001-02-20 | University Of Seville | Fuel injection nozzle and method of use |
NZ333346A (en) * | 1996-06-28 | 2000-03-27 | Caliper Techn Corp | High-throughput screening assay systems in microscale fluidic devices |
US6252129B1 (en) * | 1996-07-23 | 2001-06-26 | Electrosols, Ltd. | Dispensing device and method for forming material |
US6143248A (en) * | 1996-08-12 | 2000-11-07 | Gamera Bioscience Corp. | Capillary microvalve |
JP2002503334A (en) * | 1996-09-04 | 2002-01-29 | テクニカル ユニバーシティ オブ デンマーク | Microflow system for particle separation and analysis |
US6221654B1 (en) * | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
US6120666A (en) | 1996-09-26 | 2000-09-19 | Ut-Battelle, Llc | Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same |
JPH10217477A (en) | 1997-02-07 | 1998-08-18 | Fuji Xerox Co Ltd | Ink jet recording device |
CA2792122C (en) * | 1997-07-07 | 2015-09-08 | Medical Research Council | In vitro sorting method |
US5980936A (en) * | 1997-08-07 | 1999-11-09 | Alliance Pharmaceutical Corp. | Multiple emulsions comprising a hydrophobic continuous phase |
US6540895B1 (en) * | 1997-09-23 | 2003-04-01 | California Institute Of Technology | Microfabricated cell sorter for chemical and biological materials |
IL135151A0 (en) * | 1997-09-25 | 2001-05-20 | Ge Bayer Silicones Gmbh & Co | Device and method for producing silicone emulsions |
JP3081880B2 (en) | 1998-03-30 | 2000-08-28 | 農林水産省食品総合研究所長 | Microsphere continuous manufacturing equipment |
ATE530891T1 (en) | 1998-05-22 | 2011-11-15 | California Inst Of Techn | MINIATURIZED CELL SORTER |
US6003794A (en) | 1998-08-04 | 1999-12-21 | Progressive Grower Technologies, Inc. | Electrostatic spray module |
US6614598B1 (en) * | 1998-11-12 | 2003-09-02 | Institute Of Technology, California | Microlensing particles and applications |
US6450189B1 (en) * | 1998-11-13 | 2002-09-17 | Universidad De Sevilla | Method and device for production of components for microfabrication |
GB9900298D0 (en) * | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
US6633031B1 (en) | 1999-03-02 | 2003-10-14 | Advion Biosciences, Inc. | Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method |
DE19911777A1 (en) * | 1999-03-17 | 2000-09-21 | Merck Patent Gmbh | Process for the preparation of cosmetic formulations |
AU770678B2 (en) * | 1999-05-17 | 2004-02-26 | Caliper Life Sciences, Inc. | Focusing of microparticles in microfluidic systems |
US6592821B1 (en) * | 1999-05-17 | 2003-07-15 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
US20060169800A1 (en) * | 1999-06-11 | 2006-08-03 | Aradigm Corporation | Aerosol created by directed flow of fluids and devices and methods for producing same |
EP1192009B1 (en) * | 1999-06-11 | 2013-05-01 | Aradigm Corporation | Method for producing an aerosol |
CA2721172C (en) * | 1999-06-28 | 2012-04-10 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
US6524456B1 (en) | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
US20010050881A1 (en) | 1999-09-20 | 2001-12-13 | Depaoli David W. | Continuous flow, electrohydrodynamic micromixing apparatus and methods |
US6890487B1 (en) * | 1999-09-30 | 2005-05-10 | Science & Technology Corporation ©UNM | Flow cytometry for high throughput screening |
DE19961257C2 (en) * | 1999-12-18 | 2002-12-19 | Inst Mikrotechnik Mainz Gmbh | micromixer |
WO2001051918A1 (en) * | 2000-01-12 | 2001-07-19 | Ut-Battelle, Llc | A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream |
CN1429181A (en) * | 2000-03-10 | 2003-07-09 | 流体聚焦公司 | Methods for producing optical fiber by focusing high viscosity liquid |
US7485454B1 (en) * | 2000-03-10 | 2009-02-03 | Bioprocessors Corp. | Microreactor |
DE10015109A1 (en) * | 2000-03-28 | 2001-10-04 | Peter Walzel | Processes and devices for producing drops of equal size |
WO2001080283A1 (en) | 2000-04-18 | 2001-10-25 | Waters Investments Limited | Improved electrospray and other lc/ms interfaces |
JP2001301154A (en) | 2000-04-20 | 2001-10-30 | Dainippon Printing Co Ltd | Field jet sticking method of liquid having surface tension lowering upon application of voltage |
DE10025290B4 (en) | 2000-05-22 | 2005-03-24 | Fico I.T.M. S.A. | Sun visor outer surfaces |
US20010048637A1 (en) * | 2000-05-24 | 2001-12-06 | Weigl Bernhard H. | Microfluidic system and method |
US6645432B1 (en) * | 2000-05-25 | 2003-11-11 | President & Fellows Of Harvard College | Microfluidic systems including three-dimensionally arrayed channel networks |
US6686184B1 (en) | 2000-05-25 | 2004-02-03 | President And Fellows Of Harvard College | Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks |
US6777450B1 (en) * | 2000-05-26 | 2004-08-17 | Color Access, Inc. | Water-thin emulsions with low emulsifier levels |
US20060263888A1 (en) * | 2000-06-02 | 2006-11-23 | Honeywell International Inc. | Differential white blood count on a disposable card |
US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
US6301055B1 (en) * | 2000-08-16 | 2001-10-09 | California Institute Of Technology | Solid immersion lens structures and methods for producing solid immersion lens structures |
DE10041823C2 (en) * | 2000-08-25 | 2002-12-19 | Inst Mikrotechnik Mainz Gmbh | Method and static micromixer for mixing at least two fluids |
US6610499B1 (en) * | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
EP1334347A1 (en) * | 2000-09-15 | 2003-08-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
US6508988B1 (en) * | 2000-10-03 | 2003-01-21 | California Institute Of Technology | Combinatorial synthesis system |
US6778724B2 (en) * | 2000-11-28 | 2004-08-17 | The Regents Of The University Of California | Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices |
US20040096515A1 (en) * | 2001-12-07 | 2004-05-20 | Bausch Andreas R. | Methods and compositions for encapsulating active agents |
EP1385488A2 (en) | 2000-12-07 | 2004-02-04 | President And Fellows Of Harvard College | Methods and compositions for encapsulating active agents |
ES2180405B1 (en) | 2001-01-31 | 2004-01-16 | Univ Sevilla | DEVICE AND PROCEDURE FOR PRODUCING MULTICOMPONENT COMPOSITE LIQUID JEANS AND MULTICOMPONENT AND / OR MULTI-PAPER MICRO AND NANOMETRIC SIZE CAPSULES. |
CA2438856C (en) | 2001-02-23 | 2007-08-07 | Japan Science And Technology Corporation | Process and apparatus for producing emulsion and microcapsules |
EP1741482B1 (en) | 2001-02-23 | 2008-10-15 | Japan Science and Technology Agency | Process and apparatus for producing microcapsules |
US7037417B2 (en) * | 2001-03-19 | 2006-05-02 | Ecole Polytechnique Federale De Lausanne | Mechanical control of fluids in micro-analytical devices |
US6752922B2 (en) * | 2001-04-06 | 2004-06-22 | Fluidigm Corporation | Microfluidic chromatography |
US7318642B2 (en) | 2001-04-10 | 2008-01-15 | Essilor International (Compagnie Générale d'Optique) | Progressive addition lenses with reduced unwanted astigmatism |
ATE410680T1 (en) * | 2001-05-26 | 2008-10-15 | One Cell Systems Inc | SECRETION OF PROTEINS BY ENCAPSULATED CELLS |
GB0114854D0 (en) | 2001-06-18 | 2001-08-08 | Medical Res Council | Selective gene amplification |
US20030015425A1 (en) * | 2001-06-20 | 2003-01-23 | Coventor Inc. | Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system |
WO2003011443A2 (en) | 2001-07-27 | 2003-02-13 | President And Fellows Of Harvard College | Laminar mixing apparatus and methods |
US6520425B1 (en) * | 2001-08-21 | 2003-02-18 | The University Of Akron | Process and apparatus for the production of nanofibers |
JP4182195B2 (en) * | 2001-09-03 | 2008-11-19 | 独立行政法人農業・食品産業技術総合研究機構 | Monodispersed complex emulsion production equipment |
MXPA03006862A (en) | 2002-01-30 | 2004-10-15 | Kraft Foods Holdings Inc | Production of capsules and particles for improvement of food products. |
US7147763B2 (en) | 2002-04-01 | 2006-12-12 | Palo Alto Research Center Incorporated | Apparatus and method for using electrostatic force to cause fluid movement |
US6976590B2 (en) * | 2002-06-24 | 2005-12-20 | Cytonome, Inc. | Method and apparatus for sorting particles |
ATE479899T1 (en) * | 2002-05-09 | 2010-09-15 | Univ Chicago | EQUIPMENT AND METHODS FOR PRESSURE CONTROLLED PLUG TRANSPORT AND REACTION |
JP2006507921A (en) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | Method and apparatus for fluid dispersion |
US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
US7329545B2 (en) | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
GB2395196B (en) * | 2002-11-14 | 2006-12-27 | Univ Cardiff | Microfluidic device and methods for construction and application |
WO2004071638A2 (en) | 2003-02-11 | 2004-08-26 | Regents Of The University Of California, The | Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles |
US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
US7045040B2 (en) * | 2003-03-20 | 2006-05-16 | Asm Nutool, Inc. | Process and system for eliminating gas bubbles during electrochemical processing |
US20060078893A1 (en) * | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
EP1610888A2 (en) * | 2003-04-10 | 2006-01-04 | President And Fellows Of Harvard College | Formation and control of fluidic species |
EP1629286A1 (en) | 2003-05-16 | 2006-03-01 | Global Technologies (NZ) Ltd. | Method and apparatus for mixing sample and reagent in a suspension fluid |
WO2004103565A2 (en) | 2003-05-19 | 2004-12-02 | Hans-Knöll-Institut für Naturstoff-Forschung e.V. | Device and method for structuring liquids and for dosing reaction liquids into liquid compartments immersed in a separation medium |
JP2005037346A (en) | 2003-06-25 | 2005-02-10 | Aisin Seiki Co Ltd | Micro fluid control system |
US7115230B2 (en) * | 2003-06-26 | 2006-10-03 | Intel Corporation | Hydrodynamic focusing devices |
GB0315438D0 (en) | 2003-07-02 | 2003-08-06 | Univ Manchester | Analysis of mixed cell populations |
US20050032238A1 (en) * | 2003-08-07 | 2005-02-10 | Nanostream, Inc. | Vented microfluidic separation devices and methods |
BRPI0414004A (en) * | 2003-08-27 | 2006-10-24 | Harvard College | electronic control of fluidic species |
AU2004269406B2 (en) * | 2003-08-28 | 2010-12-16 | Progenity, Inc. | Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network |
US7204431B2 (en) * | 2003-10-31 | 2007-04-17 | Agilent Technologies, Inc. | Electrospray ion source for mass spectroscopy |
US20050103690A1 (en) | 2003-11-19 | 2005-05-19 | Aisin Seiki Kabushiki Kaisha | Micro liquid control system |
EP1691792A4 (en) | 2003-11-24 | 2008-05-28 | Yeda Res & Dev | Compositions and methods for in vitro sorting of molecular and cellular libraries |
US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
US8696952B2 (en) | 2004-04-23 | 2014-04-15 | Eugenia Kumacheva | Method of producing polymeric particles with selected size, shape, morphology and composition |
US9477233B2 (en) | 2004-07-02 | 2016-10-25 | The University Of Chicago | Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets |
EP1796828A1 (en) | 2004-07-02 | 2007-06-20 | VersaMatrix A/S | Spherical radiofrequency-encoded beads |
US7759111B2 (en) * | 2004-08-27 | 2010-07-20 | The Regents Of The University Of California | Cell encapsulation microfluidic device |
CN101052468B (en) | 2004-09-09 | 2012-02-01 | 居里研究所 | Microfluidic device using a collinear electric field |
US7968287B2 (en) * | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
US20080004436A1 (en) | 2004-11-15 | 2008-01-03 | Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science | Directed Evolution and Selection Using in Vitro Compartmentalization |
WO2006078841A1 (en) | 2005-01-21 | 2006-07-27 | President And Fellows Of Harvard College | Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles |
CA2599683A1 (en) * | 2005-03-04 | 2006-09-14 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
US20070054119A1 (en) * | 2005-03-04 | 2007-03-08 | Piotr Garstecki | Systems and methods of forming particles |
FR2882939B1 (en) * | 2005-03-11 | 2007-06-08 | Centre Nat Rech Scient | FLUIDIC SEPARATION DEVICE |
US8734003B2 (en) * | 2005-09-15 | 2014-05-27 | Alcatel Lucent | Micro-chemical mixing |
EP2363205A3 (en) | 2006-01-11 | 2014-06-04 | Raindance Technologies, Inc. | Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors |
US7537897B2 (en) | 2006-01-23 | 2009-05-26 | Population Genetics Technologies, Ltd. | Molecular counting |
DE602007009811D1 (en) | 2006-01-27 | 2010-11-25 | Harvard College | COALESCENCE FLUIDER DROPLET |
WO2007114794A1 (en) | 2006-03-31 | 2007-10-11 | Nam Trung Nguyen | Active control for droplet-based microfluidics |
EP2530168B1 (en) | 2006-05-11 | 2015-09-16 | Raindance Technologies, Inc. | Microfluidic Devices |
FR2901717A1 (en) | 2006-05-30 | 2007-12-07 | Centre Nat Rech Scient | METHOD FOR TREATING DROPS IN A MICROFLUIDIC CIRCUIT |
CN102014871A (en) | 2007-03-28 | 2011-04-13 | 哈佛大学 | Emulsions and techniques for formation |
WO2008134153A1 (en) | 2007-04-23 | 2008-11-06 | Advanced Liquid Logic, Inc. | Bead-based multiplexed analytical methods and instrumentation |
WO2009011808A1 (en) | 2007-07-13 | 2009-01-22 | President And Fellows Of Harvard College | Droplet-based selection |
EP4047367A1 (en) | 2008-07-18 | 2022-08-24 | Bio-Rad Laboratories, Inc. | Method for detecting target analytes with droplet libraries |
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
JP2010198393A (en) | 2009-02-26 | 2010-09-09 | Alpine Electronics Inc | Map display device |
CA2814049C (en) | 2010-10-08 | 2021-07-13 | President And Fellows Of Harvard College | High-throughput single cell barcoding |
-
2003
- 2003-06-03 JP JP2004549845A patent/JP2006507921A/en active Pending
- 2003-06-30 AU AU2003253751A patent/AU2003253751B2/en not_active Expired
- 2003-06-30 EP EP20030762228 patent/EP1515803A2/en not_active Withdrawn
- 2003-06-30 EP EP20100184514 patent/EP2275206A1/en not_active Ceased
- 2003-06-30 CA CA2491564A patent/CA2491564C/en not_active Expired - Lifetime
- 2003-06-30 WO PCT/US2003/020542 patent/WO2004002627A2/en active Application Filing
- 2003-06-30 CN CN2010105999339A patent/CN102059162A/en active Pending
- 2003-06-30 CN CN038204940A patent/CN1678397B/en not_active Expired - Lifetime
-
2004
- 2004-12-28 US US11/024,228 patent/US7708949B2/en active Active
-
2009
- 2009-11-20 JP JP2009265751A patent/JP5624310B2/en not_active Expired - Lifetime
-
2010
- 2010-03-17 US US12/726,223 patent/US8337778B2/en not_active Expired - Lifetime
-
2012
- 2012-11-16 US US13/679,190 patent/US8986628B2/en not_active Expired - Lifetime
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9448172B2 (en) | 2003-03-31 | 2016-09-20 | Medical Research Council | Selection by compartmentalised screening |
US9186643B2 (en) | 2004-10-08 | 2015-11-17 | Medical Research Council | In vitro evolution in microfluidic systems |
US8871444B2 (en) | 2004-10-08 | 2014-10-28 | Medical Research Council | In vitro evolution in microfluidic systems |
US9498759B2 (en) | 2004-10-12 | 2016-11-22 | President And Fellows Of Harvard College | Compartmentalized screening by microfluidic control |
US9534216B2 (en) | 2006-01-11 | 2017-01-03 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
US9410151B2 (en) | 2006-01-11 | 2016-08-09 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
US9273308B2 (en) | 2006-05-11 | 2016-03-01 | Raindance Technologies, Inc. | Selection of compartmentalized screening method |
US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
US9498761B2 (en) | 2006-08-07 | 2016-11-22 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
US9440232B2 (en) | 2007-02-06 | 2016-09-13 | Raindance Technologies, Inc. | Manipulation of fluids and reactions in microfluidic systems |
CN102648053A (en) * | 2009-10-27 | 2012-08-22 | 哈佛学院院长等 | Droplet creation techniques |
US9216414B2 (en) | 2009-11-25 | 2015-12-22 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
US9228229B2 (en) | 2010-02-12 | 2016-01-05 | Raindance Technologies, Inc. | Digital analyte analysis |
US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
US9562897B2 (en) | 2010-09-30 | 2017-02-07 | Raindance Technologies, Inc. | Sandwich assays in droplets |
US9364803B2 (en) | 2011-02-11 | 2016-06-14 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
Also Published As
Publication number | Publication date |
---|---|
JP2010075927A (en) | 2010-04-08 |
WO2004002627A2 (en) | 2004-01-08 |
CA2491564A1 (en) | 2004-01-08 |
CN1678397B (en) | 2011-02-09 |
WO2004002627A8 (en) | 2005-03-17 |
CN1678397A (en) | 2005-10-05 |
US7708949B2 (en) | 2010-05-04 |
CN102059162A (en) | 2011-05-18 |
US8337778B2 (en) | 2012-12-25 |
JP5624310B2 (en) | 2014-11-12 |
US20050172476A1 (en) | 2005-08-11 |
AU2003253751B2 (en) | 2009-10-08 |
US20140037514A1 (en) | 2014-02-06 |
CA2491564C (en) | 2013-03-19 |
WO2004002627A3 (en) | 2004-04-01 |
EP2275206A1 (en) | 2011-01-19 |
JP2006507921A (en) | 2006-03-09 |
AU2003253751A1 (en) | 2004-01-19 |
US8986628B2 (en) | 2015-03-24 |
EP1515803A2 (en) | 2005-03-23 |
US20100172803A1 (en) | 2010-07-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2004002627B1 (en) | Method and apparatus for fluid dispersion | |
JP2012520174A5 (en) | ||
US4009715A (en) | Filter for infusion and transfusion apparatus | |
US7811344B1 (en) | Double-vortex fluid separator | |
US6601613B2 (en) | Fluid circuit components based upon passive fluid dynamics | |
US8563325B1 (en) | Coaxial microreactor for particle synthesis | |
JP2006507921A5 (en) | ||
AU2006280554B2 (en) | Hydrocyclone | |
Wu et al. | A 3D easily-assembled Micro-Cross for droplet generation | |
US5460449A (en) | In-line mixer for dispersions | |
AU6426899A (en) | Fluid circuit components based upon passive fluid dynamics | |
JP2001232112A (en) | Method and device for filtering impurities, base plate, conduit and assembly | |
JPS60227820A (en) | Rapid inline mixer of two fluids | |
CA2675163A1 (en) | Device and method for separating a flowing medium mixture with a stationary cyclone | |
EP3609346B1 (en) | Apparatus and method for generating and mixing ultrafine gas bubbles into a high gas concentration aqueous solution | |
CA3120242A1 (en) | Apparatus in the form of a unitary, single-piece structure configured to generate and mix ultra-fine gas bubbles into a high gas concentration aqueous solution | |
CN113058669A (en) | Coaxial focusing micro-channel integrated device and method capable of being customized according to requirements | |
CN111068799A (en) | Microfluidic channel for generating droplets and use thereof | |
JP2023550090A (en) | Methods and systems for mechanoporation-based payload delivery to living cells | |
CN110290774B (en) | Cosmetic composition preparation device containing emulsified material instantaneously emulsified by using micro-fluid channel | |
US11014054B2 (en) | Fluid-gas mixer | |
CN114653414B (en) | Interference fit assisted positioning capillary microfluidic device and preparation method thereof | |
CN108722027B (en) | Inertial separation device and liquid collector thereof | |
WO2003025378A3 (en) | Device for mixing two flows of fluid, which are initially guided separate from one another, in a two-circuit reaction engine | |
CN209033948U (en) | Inertial separator and its liquid trap |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
B | Later publication of amended claims |
Effective date: 20040406 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2003253751 Country of ref document: AU Ref document number: 537478 Country of ref document: NZ Ref document number: 2004549845 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 11024228 Country of ref document: US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2491564 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2003762228 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 20038204940 Country of ref document: CN |
|
CFP | Corrected version of a pamphlet front page | ||
CR1 | Correction of entry in section i |
Free format text: IN PCT GAZETTE 02/2004 UNDER (22) REPLACE "03 JUNE 2003 (03.06.03)" BY "03 JUNE 2003 (30.06.03)" |
|
WWP | Wipo information: published in national office |
Ref document number: 2003762228 Country of ref document: EP |