WO2004011900A3 - Detecting receptor oligomerization - Google Patents

Detecting receptor oligomerization Download PDF

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Publication number
WO2004011900A3
WO2004011900A3 PCT/US2003/022611 US0322611W WO2004011900A3 WO 2004011900 A3 WO2004011900 A3 WO 2004011900A3 US 0322611 W US0322611 W US 0322611W WO 2004011900 A3 WO2004011900 A3 WO 2004011900A3
Authority
WO
WIPO (PCT)
Prior art keywords
probes
binding
molecules
probe includes
cleaving
Prior art date
Application number
PCT/US2003/022611
Other languages
French (fr)
Other versions
WO2004011900A2 (en
Inventor
Po-Ying Chan-Hui
Yining Shi
Sailaja Pidaparthi
Rajiv Dua
Sharat Singh
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to DE60329194T priority Critical patent/DE60329194D1/en
Priority to JP2004524655A priority patent/JP4443407B2/en
Priority to AT03771670T priority patent/ATE442589T1/en
Priority to CA2490654A priority patent/CA2490654C/en
Priority to AU2003259175A priority patent/AU2003259175B2/en
Priority to EP03771670A priority patent/EP1540347B1/en
Publication of WO2004011900A2 publication Critical patent/WO2004011900A2/en
Publication of WO2004011900A3 publication Critical patent/WO2004011900A3/en
Priority to NO20050916A priority patent/NO20050916L/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Abstract

Methods are provided for detecting formation of oligomeric complexes of molecules on the surface of cell membranes. These methods employ pairs of tagged probes and cleaving probes, each of which binds specificly to a cell surface molecule. The tagged probe includes a molecular tag that is linked to a first binding compound through a cleavable linkage, and the cleaving probe includes a second binding agent and a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to cell surface molecules that have formed an oligomeric complex results in release of the molecular tag from the binding compound, providing a measure of formation of the complex.
PCT/US2003/022611 2002-07-25 2003-07-17 Detecting receptor oligomerization WO2004011900A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE60329194T DE60329194D1 (en) 2002-07-25 2003-07-17 PROOF OF RECEPTOROLIGERIZATION
JP2004524655A JP4443407B2 (en) 2002-07-25 2003-07-17 Detection of receptor oligomer formation
AT03771670T ATE442589T1 (en) 2002-07-25 2003-07-17 DETECTION OF RECEPTOR OLIGOMERIZATION
CA2490654A CA2490654C (en) 2002-07-25 2003-07-17 Detecting receptor oligomerization
AU2003259175A AU2003259175B2 (en) 2002-07-25 2003-07-17 Detecting receptor oligomerization
EP03771670A EP1540347B1 (en) 2002-07-25 2003-07-17 Detecting receptor oligomerization
NO20050916A NO20050916L (en) 2002-07-25 2005-02-21 Detection of receptor oligomerization

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39872402P 2002-07-25 2002-07-25
US60/398,724 2002-07-25

Publications (2)

Publication Number Publication Date
WO2004011900A2 WO2004011900A2 (en) 2004-02-05
WO2004011900A3 true WO2004011900A3 (en) 2004-06-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/022611 WO2004011900A2 (en) 2002-07-25 2003-07-17 Detecting receptor oligomerization

Country Status (13)

Country Link
US (2) US7105308B2 (en)
EP (1) EP1540347B1 (en)
JP (2) JP4443407B2 (en)
KR (1) KR20050025669A (en)
CN (1) CN1672054A (en)
AT (1) ATE442589T1 (en)
AU (1) AU2003259175B2 (en)
CA (1) CA2490654C (en)
DE (1) DE60329194D1 (en)
ES (1) ES2332310T3 (en)
NO (1) NO20050916L (en)
PL (1) PL374875A1 (en)
WO (1) WO2004011900A2 (en)

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CN104458708A (en) * 2013-09-12 2015-03-25 中国药科大学 High flux screening method for screening acetylcholine esterase inhibitor
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

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CN103012193B (en) * 2012-12-14 2015-08-19 无锡中德伯尔生物技术有限公司 Leucogentian violet haptens, artificial antigen and preparation method
US20160041171A1 (en) * 2013-04-05 2016-02-11 Laboratory Corporation Of America Holdings Systems and methods for facilitating diagnosis, prognosis and treatment of cancer based on detection of her3 activation
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use
CN104458708A (en) * 2013-09-12 2015-03-25 中国药科大学 High flux screening method for screening acetylcholine esterase inhibitor

Also Published As

Publication number Publication date
EP1540347B1 (en) 2009-09-09
ATE442589T1 (en) 2009-09-15
US20040126818A1 (en) 2004-07-01
AU2003259175A1 (en) 2004-02-16
JP4443407B2 (en) 2010-03-31
JP2009271087A (en) 2009-11-19
ES2332310T3 (en) 2010-02-02
PL374875A1 (en) 2005-11-14
CN1672054A (en) 2005-09-21
WO2004011900A2 (en) 2004-02-05
EP1540347A2 (en) 2005-06-15
US7105308B2 (en) 2006-09-12
NO20050916L (en) 2005-04-14
CA2490654C (en) 2012-02-07
CA2490654A1 (en) 2004-02-05
KR20050025669A (en) 2005-03-14
US20040197835A1 (en) 2004-10-07
JP2005534030A (en) 2005-11-10
DE60329194D1 (en) 2009-10-22
US7135300B2 (en) 2006-11-14
EP1540347A4 (en) 2006-09-20
AU2003259175B2 (en) 2010-08-12

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