WO2004024283A3 - Apparatus and method for preparative scale purification of nucleic acids - Google Patents
Apparatus and method for preparative scale purification of nucleic acids Download PDFInfo
- Publication number
- WO2004024283A3 WO2004024283A3 PCT/US2003/028759 US0328759W WO2004024283A3 WO 2004024283 A3 WO2004024283 A3 WO 2004024283A3 US 0328759 W US0328759 W US 0328759W WO 2004024283 A3 WO2004024283 A3 WO 2004024283A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acids
- preparative scale
- scale purification
- chromosomal dna
- lysates
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03D—FLOTATION; DIFFERENTIAL SEDIMENTATION
- B03D3/00—Differential sedimentation
- B03D3/06—Flocculation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP03749647A EP1554398A4 (en) | 2002-09-13 | 2003-09-12 | Apparatus and method for preparative scale purification of nucleic acids |
US10/527,618 US7314746B2 (en) | 2002-09-13 | 2003-09-12 | Apparatus and method for preparative scale purification of nucleic acids |
AU2003267175A AU2003267175A1 (en) | 2002-09-13 | 2003-09-12 | Apparatus and method for preparative scale purification of nucleic acids |
JP2004536251A JP2005538717A (en) | 2002-09-13 | 2003-09-12 | Apparatus and method for preparative scale purification of nucleic acids |
CA002498518A CA2498518A1 (en) | 2002-09-13 | 2003-09-12 | Apparatus and method for preparative scale purification of nucleic acids |
US11/965,607 US7771945B2 (en) | 2002-09-13 | 2007-12-27 | Apparatus and method for preparative scale purification of nucleic acids |
US12/850,969 US8158348B2 (en) | 2002-09-13 | 2010-08-05 | Apparatus and method for preparative scale purification of nucleic acids |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41061702P | 2002-09-13 | 2002-09-13 | |
US60/410,617 | 2002-09-13 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10527618 A-371-Of-International | 2003-09-12 | ||
US11/965,607 Division US7771945B2 (en) | 2002-09-13 | 2007-12-27 | Apparatus and method for preparative scale purification of nucleic acids |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004024283A2 WO2004024283A2 (en) | 2004-03-25 |
WO2004024283A3 true WO2004024283A3 (en) | 2004-08-05 |
Family
ID=31994168
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/028759 WO2004024283A2 (en) | 2002-09-13 | 2003-09-12 | Apparatus and method for preparative scale purification of nucleic acids |
Country Status (6)
Country | Link |
---|---|
US (3) | US7314746B2 (en) |
EP (1) | EP1554398A4 (en) |
JP (1) | JP2005538717A (en) |
AU (1) | AU2003267175A1 (en) |
CA (1) | CA2498518A1 (en) |
WO (1) | WO2004024283A2 (en) |
Families Citing this family (21)
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DE10207170A1 (en) * | 2002-02-20 | 2003-09-04 | Qiagen Gmbh | Process for the rough clarification of cell disruption from microorganisms |
US7314746B2 (en) * | 2002-09-13 | 2008-01-01 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
DK1628749T3 (en) | 2003-05-30 | 2019-09-16 | Vgxi Inc | Devices and methods for biomaterial production |
AU2004272748C1 (en) * | 2003-09-17 | 2010-07-08 | Centelion | Method of preparation of pharmaceutically grade plasmid DNA |
DE102004052254A1 (en) * | 2004-05-03 | 2005-12-01 | Plasmid Factory Gmbh & Co. Kg | Process for disrupting cells and separating cell components |
US7444197B2 (en) * | 2004-05-06 | 2008-10-28 | Smp Logic Systems Llc | Methods, systems, and software program for validation and monitoring of pharmaceutical manufacturing processes |
WO2006073472A2 (en) * | 2004-05-21 | 2006-07-13 | Mo Bio Laboratories, Inc. | Kits and processes for removing contaminants from nucleic acids in environmental and biological samples |
TW200634145A (en) * | 2005-01-25 | 2006-10-01 | Wyeth Res Ireland Ltd | Shear protectants in harvest microfiltration |
DE102005047736B4 (en) * | 2005-09-29 | 2008-08-14 | Aj Innuscreen Gmbh | Method and system for isolating nucleic acids from any complex starting materials |
AU2008256681B2 (en) * | 2007-05-23 | 2014-07-31 | Vgxi, Inc | Compositions comprising high concentration of biologically active molecules and processes for preparing the same |
EP2088196A1 (en) * | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Methods and devices for producing biomolecules |
US7932356B1 (en) * | 2010-06-23 | 2011-04-26 | Bing Lou Wong | Method for the preparation of a heat stable oxygen carrier-containing pharmaceutical composition |
GB201103138D0 (en) * | 2011-02-23 | 2011-04-06 | Msd Biolog Uk Ltd | Cell lysis process |
US20140324360A1 (en) * | 2011-09-21 | 2014-10-30 | Millennium Pharmaceuticals, Inc. | Anti-gcc antibody molecules and related compositions and methods |
EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
US9556427B2 (en) * | 2013-02-27 | 2017-01-31 | Syngenta Participations Ag | Methods and compositions for preparation of nucleic acids |
WO2015057810A1 (en) * | 2013-10-15 | 2015-04-23 | Ibis Biosciences, Inc. | Blood sampling |
EP3662063A4 (en) * | 2017-08-04 | 2021-04-28 | MicrobeDx, Inc. | Methods for lysis of cells within a sample |
WO2019035998A1 (en) * | 2017-08-17 | 2019-02-21 | Modernatx, Inc. | Systems with fluid path control and regulation |
EP3594338A1 (en) * | 2018-07-12 | 2020-01-15 | Kaneka Eurogentec SA | Method and apparatus for the purification of extra-chromosomal nucleic acid sequences |
WO2021198281A1 (en) * | 2020-03-31 | 2021-10-07 | Richter-Helm Biologics Gmbh & Co. Kg | Methods for producing plasmid dna |
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US20010045359A1 (en) * | 1996-09-06 | 2001-11-29 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
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-
2003
- 2003-09-12 US US10/527,618 patent/US7314746B2/en not_active Expired - Fee Related
- 2003-09-12 EP EP03749647A patent/EP1554398A4/en not_active Withdrawn
- 2003-09-12 WO PCT/US2003/028759 patent/WO2004024283A2/en active Application Filing
- 2003-09-12 JP JP2004536251A patent/JP2005538717A/en active Pending
- 2003-09-12 CA CA002498518A patent/CA2498518A1/en not_active Abandoned
- 2003-09-12 AU AU2003267175A patent/AU2003267175A1/en not_active Abandoned
-
2007
- 2007-12-27 US US11/965,607 patent/US7771945B2/en not_active Expired - Fee Related
-
2010
- 2010-08-05 US US12/850,969 patent/US8158348B2/en active Active
Patent Citations (2)
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---|---|---|---|---|
US20010045359A1 (en) * | 1996-09-06 | 2001-11-29 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US6036940A (en) * | 1996-11-12 | 2000-03-14 | The University Of Akron | Compositions and methods relating to the production, isolation, and modification of gas vesicles |
Non-Patent Citations (1)
Title |
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See also references of EP1554398A4 * |
Also Published As
Publication number | Publication date |
---|---|
AU2003267175A1 (en) | 2004-04-30 |
US7771945B2 (en) | 2010-08-10 |
EP1554398A2 (en) | 2005-07-20 |
US8158348B2 (en) | 2012-04-17 |
EP1554398A4 (en) | 2005-12-14 |
US20060166331A1 (en) | 2006-07-27 |
US7314746B2 (en) | 2008-01-01 |
US20080102519A1 (en) | 2008-05-01 |
US20110070638A1 (en) | 2011-03-24 |
JP2005538717A (en) | 2005-12-22 |
WO2004024283A2 (en) | 2004-03-25 |
CA2498518A1 (en) | 2004-03-25 |
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