WO2004024886A3 - Microarray synthesis and assembly of gene-length polynucleotides - Google Patents

Microarray synthesis and assembly of gene-length polynucleotides Download PDF

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Publication number
WO2004024886A3
WO2004024886A3 PCT/US2003/028946 US0328946W WO2004024886A3 WO 2004024886 A3 WO2004024886 A3 WO 2004024886A3 US 0328946 W US0328946 W US 0328946W WO 2004024886 A3 WO2004024886 A3 WO 2004024886A3
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Prior art keywords
assembly
gene
length polynucleotides
synthesis
microarray synthesis
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PCT/US2003/028946
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French (fr)
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WO2004024886A2 (en
Inventor
Andrew V Oleinikov
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Combimatrix Corp
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Publication date
Application filed by Combimatrix Corp filed Critical Combimatrix Corp
Priority to EP03752373.5A priority Critical patent/EP1546387B1/en
Priority to CA2498746A priority patent/CA2498746C/en
Priority to AU2003270666A priority patent/AU2003270666B2/en
Priority to JP2004536347A priority patent/JP4783874B2/en
Priority to EP20189157.9A priority patent/EP3770270A1/en
Priority to EP15189572.9A priority patent/EP3059321B1/en
Publication of WO2004024886A2 publication Critical patent/WO2004024886A2/en
Publication of WO2004024886A3 publication Critical patent/WO2004024886A3/en

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
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    • B01J2219/00709—Type of synthesis
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    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
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    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Abstract

There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, 'on device' assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
PCT/US2003/028946 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides WO2004024886A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP03752373.5A EP1546387B1 (en) 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides
CA2498746A CA2498746C (en) 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides
AU2003270666A AU2003270666B2 (en) 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides
JP2004536347A JP4783874B2 (en) 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides
EP20189157.9A EP3770270A1 (en) 2002-09-12 2003-09-12 Microassay synthesis and assembly of gene-length polynucleotides
EP15189572.9A EP3059321B1 (en) 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/243,367 US7563600B2 (en) 2002-09-12 2002-09-12 Microarray synthesis and assembly of gene-length polynucleotides
US10/243,367 2002-09-12

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WO2004024886A2 WO2004024886A2 (en) 2004-03-25
WO2004024886A3 true WO2004024886A3 (en) 2005-01-20

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PCT/US2003/028946 WO2004024886A2 (en) 2002-09-12 2003-09-12 Microarray synthesis and assembly of gene-length polynucleotides

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US (9) US7563600B2 (en)
EP (4) EP2330216B1 (en)
JP (1) JP4783874B2 (en)
AU (1) AU2003270666B2 (en)
CA (2) CA2498746C (en)
DK (1) DK3059321T3 (en)
WO (1) WO2004024886A2 (en)

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US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis

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US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
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