WO2004044156A3 - Transgenic ungulates having reduced prion protein activity and uses thereof - Google Patents

Transgenic ungulates having reduced prion protein activity and uses thereof Download PDF

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Publication number
WO2004044156A3
WO2004044156A3 PCT/US2003/035720 US0335720W WO2004044156A3 WO 2004044156 A3 WO2004044156 A3 WO 2004044156A3 US 0335720 W US0335720 W US 0335720W WO 2004044156 A3 WO2004044156 A3 WO 2004044156A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein activity
prion protein
bovines
transgenic ungulates
transgenic
Prior art date
Application number
PCT/US2003/035720
Other languages
French (fr)
Other versions
WO2004044156A2 (en
Inventor
James Robl
Yoshimi Kuroiwa
Philippe Collas
Eddie Sullivan
Poothappillai Kasinathan
Kazuma Tomizuka
Isao Ishida
Original Assignee
Hematech Llc
Kirin Brewery
James Robl
Yoshimi Kuroiwa
Philippe Collas
Eddie Sullivan
Poothappillai Kasinathan
Kazuma Tomizuka
Isao Ishida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hematech Llc, Kirin Brewery, James Robl, Yoshimi Kuroiwa, Philippe Collas, Eddie Sullivan, Poothappillai Kasinathan, Kazuma Tomizuka, Isao Ishida filed Critical Hematech Llc
Priority to JP2005507127A priority Critical patent/JP2006505291A/en
Priority to BR0315300-2A priority patent/BR0315300A/en
Priority to MXPA05004870A priority patent/MXPA05004870A/en
Priority to CA002502490A priority patent/CA2502490A1/en
Priority to EP03783271A priority patent/EP1563084A4/en
Priority to AU2003290689A priority patent/AU2003290689A1/en
Priority to NZ539459A priority patent/NZ539459A/en
Publication of WO2004044156A2 publication Critical patent/WO2004044156A2/en
Publication of WO2004044156A3 publication Critical patent/WO2004044156A3/en
Priority to IL206781A priority patent/IL206781A0/en
Priority to AU2010224312A priority patent/AU2010224312A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0271—Chimeric animals, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knockout animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Humanized animals, e.g. knockin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/12—Immunoglobulins specific features characterized by their source of isolation or production isolated from milk
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Abstract

The invention provides cloned transgenic ungulates (e.g., bovines) in which prion protein activity is reduced by one or more genetically engineered mutations. Desirably, these transgenic bovines are also genetically modified to express xenogenous (e.g., human) antibodies. Because of their resistance to prion-related diseases such as bovine spongiform encephalopy (also known as mad cow disease), these bovines are a safer source of human antibodies for pharmaceutical uses and safer source of agricultural products.
PCT/US2003/035720 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof WO2004044156A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP2005507127A JP2006505291A (en) 2002-11-08 2003-11-10 Transgenic ungulates with reduced prion protein activity and uses thereof
BR0315300-2A BR0315300A (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and its uses
MXPA05004870A MXPA05004870A (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof.
CA002502490A CA2502490A1 (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof
EP03783271A EP1563084A4 (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof
AU2003290689A AU2003290689A1 (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof
NZ539459A NZ539459A (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof
IL206781A IL206781A0 (en) 2002-11-08 2010-07-04 Transgenic bovine with a genome comprising a non-natural mutation with reduced functional prion production, and a method for producing antibodies therefrom
AU2010224312A AU2010224312A1 (en) 2002-11-08 2010-09-17 Transgenic ungulates having reduced prion protein activity and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US42505602P 2002-11-08 2002-11-08
US60/425,056 2002-11-08
US50690103P 2003-09-26 2003-09-26
US60/506,901 2003-09-26

Publications (2)

Publication Number Publication Date
WO2004044156A2 WO2004044156A2 (en) 2004-05-27
WO2004044156A3 true WO2004044156A3 (en) 2004-07-29

Family

ID=32314569

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/035720 WO2004044156A2 (en) 2002-11-08 2003-11-10 Transgenic ungulates having reduced prion protein activity and uses thereof

Country Status (12)

Country Link
US (2) US7429690B2 (en)
EP (1) EP1563084A4 (en)
JP (1) JP2006505291A (en)
KR (1) KR20050093761A (en)
AU (2) AU2003290689A1 (en)
BR (1) BR0315300A (en)
CA (1) CA2502490A1 (en)
IL (1) IL206781A0 (en)
MX (1) MXPA05004870A (en)
NZ (1) NZ539459A (en)
RU (1) RU2384059C2 (en)
WO (1) WO2004044156A2 (en)

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US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
WO2001035735A1 (en) * 1999-11-19 2001-05-25 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
CA2502490A1 (en) 2002-11-08 2004-05-27 Hematech, Llc Transgenic ungulates having reduced prion protein activity and uses thereof
JP2007533328A (en) * 2004-04-22 2007-11-22 キリンホールディングス株式会社 Transgenic animals and uses thereof
US20060130157A1 (en) 2004-10-22 2006-06-15 Kevin Wells Ungulates with genetically modified immune systems
WO2006067847A1 (en) * 2004-12-22 2006-06-29 Chugai Seiyaku Kabushiki Kaisha Method of preparing antibody by use of cell having its fucose transporter function inhibited
KR100710008B1 (en) * 2006-02-02 2007-04-23 충남대학교산학협력단 Method for improvement of cloning efficiency by treatment of somatic cell with streptolysin o
ES2548377T3 (en) 2008-10-27 2015-10-16 Revivicor, Inc. Immunosuppressed ungulates
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