WO2004063716A3 - Assay products and procedures - Google Patents

Assay products and procedures Download PDF

Info

Publication number
WO2004063716A3
WO2004063716A3 PCT/US2004/000619 US2004000619W WO2004063716A3 WO 2004063716 A3 WO2004063716 A3 WO 2004063716A3 US 2004000619 W US2004000619 W US 2004000619W WO 2004063716 A3 WO2004063716 A3 WO 2004063716A3
Authority
WO
WIPO (PCT)
Prior art keywords
particles
class
pair
procedures
labels
Prior art date
Application number
PCT/US2004/000619
Other languages
French (fr)
Other versions
WO2004063716A2 (en
Inventor
Michael L Bell
Original Assignee
Beckman Coulter Inc
Michael L Bell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc, Michael L Bell filed Critical Beckman Coulter Inc
Publication of WO2004063716A2 publication Critical patent/WO2004063716A2/en
Publication of WO2004063716A3 publication Critical patent/WO2004063716A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Abstract

In cytometry and like procedures, the number of different types of identifiable particle (and, therefore, the number of detectable analytes) is increased by using the coding labels on some particles as signal labels on other particles, and by using the signal labels on some particles as coding labels on other particles. In one such procedure, the particles of a first class are coded with preselected amounts of a first pair of fluorochromes, and the particles of a second class of particles are coded with preselected amounts of a second pair of fluorochromes. The analytes which interact with the first class of particles are identified by a signal label selected from the second pair; and the analytes which interact with a second class of particles are identified by a signal label selected from the first pair.
PCT/US2004/000619 2003-01-10 2004-01-09 Assay products and procedures WO2004063716A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/340,278 US7326573B2 (en) 2003-01-10 2003-01-10 Assay procedures and apparatus
US10/340,278 2003-01-10

Publications (2)

Publication Number Publication Date
WO2004063716A2 WO2004063716A2 (en) 2004-07-29
WO2004063716A3 true WO2004063716A3 (en) 2004-11-11

Family

ID=32711290

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/000619 WO2004063716A2 (en) 2003-01-10 2004-01-09 Assay products and procedures

Country Status (2)

Country Link
US (1) US7326573B2 (en)
WO (1) WO2004063716A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7722537B2 (en) * 2005-02-14 2010-05-25 Optiscan Biomedical Corp. Method and apparatus for detection of multiple analytes
US8597190B2 (en) 2007-05-18 2013-12-03 Optiscan Biomedical Corporation Monitoring systems and methods with fast initialization
US9091676B2 (en) 2010-06-09 2015-07-28 Optiscan Biomedical Corp. Systems and methods for measuring multiple analytes in a sample
US8603828B2 (en) 2009-11-18 2013-12-10 Bio-Rad Laboratories, Inc. Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Citations (6)

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EP0121262A2 (en) * 1983-04-05 1984-10-10 Becton Dickinson and Company Method and apparatus for distinguishing multiple subpopulations of cells in a sample
EP0219309A1 (en) * 1985-10-11 1987-04-22 Smithkline Beecham Corporation Methods and reagents for performing analyses of subpopulations of particles
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
WO2000067894A2 (en) * 1999-05-12 2000-11-16 Axys Pharmaceuticals, Inc. Methods of software driven flow sorting for reiterative synthesis cycles
US6225046B1 (en) * 1995-04-03 2001-05-01 Macquarie Research Ltd. Method for detecting microorganisms
US20020115116A1 (en) * 2001-02-22 2002-08-22 Yong Song Multiplex protein interaction determinations using glutathione-GST binding

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
US4499052A (en) 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
DE3322373C2 (en) 1983-05-19 1986-12-04 Ioannis Dr. 3000 Hannover Tripatzis Test means and methods for the detection of antigens and / or antibodies
US4699828A (en) 1984-04-27 1987-10-13 Becton, Dickinson And Company Fluorescently labeled microbeads
US4665020A (en) 1984-05-30 1987-05-12 United States Department Of Energy Flow cytometer measurement of binding assays
US5073497A (en) 1989-06-30 1991-12-17 Caribbean Microparticles Corporation Microbead reference standard and method of adjusting a flow cytometer to obtain reproducible results using the microbeads
SE458968B (en) 1987-06-16 1989-05-22 Wallac Oy BIOSPECIFIC ANALYTICAL PROCEDURE FOR MULTIPLE ANALYTICS WHICH DO NOT INCLUDE PARTICULAR COATING AND LABELING WITH FLUORESCING LABEL SUBSTANCES
US5385822A (en) 1988-05-02 1995-01-31 Zynaxis, Inc. Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population
US5646001A (en) 1991-03-25 1997-07-08 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
US5674698A (en) * 1992-09-14 1997-10-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5556764A (en) * 1993-02-17 1996-09-17 Biometric Imaging, Inc. Method and apparatus for cell counting and cell classification
US6159748A (en) 1995-03-13 2000-12-12 Affinitech, Ltd Evaluation of autoimmune diseases using a multiple parameter latex bead suspension and flow cytometry
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5747349A (en) 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
EP0920627B1 (en) 1996-06-07 2004-05-12 Immunivest Corporation Magnetic separation employing external and internal gradients
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
CA2260991C (en) 1997-11-18 2009-03-24 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
US6165796A (en) 1997-11-26 2000-12-26 Beckman Coulter, Inc. Pipettable ion detector and method
DE60040603D1 (en) 1999-08-17 2008-12-04 Luminex Corp METHOD FOR ANALYZING A MULTIPLE OF SAMPLES OF VARIOUS ORIGIN ON AN ANALYTE

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0121262A2 (en) * 1983-04-05 1984-10-10 Becton Dickinson and Company Method and apparatus for distinguishing multiple subpopulations of cells in a sample
EP0219309A1 (en) * 1985-10-11 1987-04-22 Smithkline Beecham Corporation Methods and reagents for performing analyses of subpopulations of particles
US6225046B1 (en) * 1995-04-03 2001-05-01 Macquarie Research Ltd. Method for detecting microorganisms
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
WO2000067894A2 (en) * 1999-05-12 2000-11-16 Axys Pharmaceuticals, Inc. Methods of software driven flow sorting for reiterative synthesis cycles
US20020115116A1 (en) * 2001-02-22 2002-08-22 Yong Song Multiplex protein interaction determinations using glutathione-GST binding

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LUTZ E S M ET AL: "Implementation of affinity solid-phases in continuous-flow biochemical detection", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 776, no. 2, 1 August 1997 (1997-08-01), pages 169 - 178, XP004125594, ISSN: 0021-9673 *
NANTHAKUMAR A ET AL: "Solid-phase oligonucleotide synthesis and flow cytometric Analysis with Microspheres Encoded with Covalently Attached Fluorophores", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 11, 2000, pages 282 - 288, XP002159465, ISSN: 1043-1802 *

Also Published As

Publication number Publication date
US7326573B2 (en) 2008-02-05
WO2004063716A2 (en) 2004-07-29
US20040137635A1 (en) 2004-07-15

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