WO2004096984A3 - Preservation of rna in a biological sample - Google Patents

Preservation of rna in a biological sample Download PDF

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Publication number
WO2004096984A3
WO2004096984A3 PCT/US2004/011821 US2004011821W WO2004096984A3 WO 2004096984 A3 WO2004096984 A3 WO 2004096984A3 US 2004011821 W US2004011821 W US 2004011821W WO 2004096984 A3 WO2004096984 A3 WO 2004096984A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
preservation
biological sample
sample
violet
Prior art date
Application number
PCT/US2004/011821
Other languages
French (fr)
Other versions
WO2004096984A2 (en
Inventor
Fredrik C Kamme
Bernhard H Meurers
Dmitri Talantov
Jingxue Yu
Original Assignee
Janssen Pharmaceutica Nv
Fredrik C Kamme
Bernhard H Meurers
Dmitri Talantov
Jingxue Yu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Pharmaceutica Nv, Fredrik C Kamme, Bernhard H Meurers, Dmitri Talantov, Jingxue Yu filed Critical Janssen Pharmaceutica Nv
Priority to MXPA05011404A priority Critical patent/MXPA05011404A/en
Priority to AU2004235298A priority patent/AU2004235298A1/en
Priority to CA002523501A priority patent/CA2523501A1/en
Priority to EP04760270A priority patent/EP1623010A4/en
Publication of WO2004096984A2 publication Critical patent/WO2004096984A2/en
Publication of WO2004096984A3 publication Critical patent/WO2004096984A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Abstract

To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods.
PCT/US2004/011821 2003-04-25 2004-04-15 Preservation of rna in a biological sample WO2004096984A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
MXPA05011404A MXPA05011404A (en) 2003-04-25 2004-04-15 Preservation of rna in a biological sample.
AU2004235298A AU2004235298A1 (en) 2003-04-25 2004-04-15 Preservation of RNA in a biological sample
CA002523501A CA2523501A1 (en) 2003-04-25 2004-04-15 Preservation of rna in a biological sample
EP04760270A EP1623010A4 (en) 2003-04-25 2004-04-15 Preservation of rna in a biological sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46530703P 2003-04-25 2003-04-25
US60/465,307 2003-04-25

Publications (2)

Publication Number Publication Date
WO2004096984A2 WO2004096984A2 (en) 2004-11-11
WO2004096984A3 true WO2004096984A3 (en) 2005-06-02

Family

ID=33418220

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/011821 WO2004096984A2 (en) 2003-04-25 2004-04-15 Preservation of rna in a biological sample

Country Status (6)

Country Link
US (2) US7056673B2 (en)
EP (1) EP1623010A4 (en)
AU (1) AU2004235298A1 (en)
CA (1) CA2523501A1 (en)
MX (1) MXPA05011404A (en)
WO (1) WO2004096984A2 (en)

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US8030034B2 (en) 2005-12-09 2011-10-04 Promega Corporation Nucleic acid purification with a binding matrix

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US20070249821A1 (en) * 2006-03-08 2007-10-25 Promega Corporation Small RNA purification
WO2010028288A2 (en) 2008-09-05 2010-03-11 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
CN102203604B (en) * 2008-10-21 2014-09-10 托马斯·M·东德林格 Methods and compositions for preventing artifacts in tissue samples
WO2010088364A2 (en) 2009-01-30 2010-08-05 L'air Liquide Societe Anonyme Pour L'etude Et L'exploitation Des Procedes Georges Claude Process for preserving biological materials for extended periods of time
AU2010257118B2 (en) 2009-06-04 2014-08-28 Lockheed Martin Corporation Multiple-sample microfluidic chip for DNA analysis
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
GB2497501A (en) 2010-10-15 2013-06-12 Lockheed Corp Micro fluidic optic design
EP3197336B1 (en) 2014-09-25 2020-12-23 Progenity, Inc. Electromechanical pill device with localization capabilities
CN109890299A (en) 2016-08-18 2019-06-14 普罗根尼蒂公司 Sampling system and associated materials and method
JP2019526423A (en) 2016-09-09 2019-09-19 ミッチェル・ローレンス・ジョーンズＭｉｔｃｈｅｌｌ Ｌａｗｒｅｎｃｅ ＪＯＮＥＳ Electromechanically ingestible device for delivery of dispenseable substances
WO2018106931A1 (en) 2016-12-07 2018-06-14 Progenity Inc. Gastrointestinal tract detection methods, devices and systems
CA3055762A1 (en) 2017-03-31 2018-10-04 Progenity, Inc. Localization systems and methods for an ingestible device
CN112400024B (en) 2018-06-01 2024-04-09 比奥拉治疗股份有限公司 Device and system for gastrointestinal microbiome detection and processing
EP3883635A1 (en) 2018-11-19 2021-09-29 Progenity, Inc. Methods and devices for treating a disease with biotherapeutics

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US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
US20030152928A1 (en) * 2001-04-11 2003-08-14 Satoshi Asai Method of screening a gene
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US20020197637A1 (en) * 2001-06-02 2002-12-26 Willson Richard C. Process and compositions for protection of nucleic acids

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8030034B2 (en) 2005-12-09 2011-10-04 Promega Corporation Nucleic acid purification with a binding matrix

Also Published As

Publication number Publication date
MXPA05011404A (en) 2006-05-31
WO2004096984A2 (en) 2004-11-11
US20040259133A1 (en) 2004-12-23
EP1623010A4 (en) 2007-12-26
US7056673B2 (en) 2006-06-06
US20060166258A1 (en) 2006-07-27
AU2004235298A1 (en) 2004-11-11
CA2523501A1 (en) 2004-11-11
EP1623010A2 (en) 2006-02-08

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