WO2004112074A3 - Laser desorption ion source - Google Patents

Laser desorption ion source Download PDF

Info

Publication number
WO2004112074A3
WO2004112074A3 PCT/US2004/017832 US2004017832W WO2004112074A3 WO 2004112074 A3 WO2004112074 A3 WO 2004112074A3 US 2004017832 W US2004017832 W US 2004017832W WO 2004112074 A3 WO2004112074 A3 WO 2004112074A3
Authority
WO
WIPO (PCT)
Prior art keywords
electric field
desorption
sample
ion
laser
Prior art date
Application number
PCT/US2004/017832
Other languages
French (fr)
Other versions
WO2004112074A2 (en
Inventor
Ross C Willoughby
Edward W Sheehan
Craig M Whitehouse
Original Assignee
Ross C Willoughby
Edward W Sheehan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ross C Willoughby, Edward W Sheehan filed Critical Ross C Willoughby
Priority to EP04754443.2A priority Critical patent/EP1639622B1/en
Priority to CA2527886A priority patent/CA2527886C/en
Publication of WO2004112074A2 publication Critical patent/WO2004112074A2/en
Publication of WO2004112074A3 publication Critical patent/WO2004112074A3/en

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
    • H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0031—Step by step routines describing the use of the apparatus
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/06—Electron- or ion-optical arrangements

Abstract

Atmospheric pressure, intermediate pressure and vacuum laser desorption ionization methods and ion sources (1) are configured to increase ionization efficiency and the efficiency of transmitting ions to a mass to charge analyzer or ion mobility analyzer. An electric field is applied in the region of a sample target (5) to accumulate ions generated from a local ion source (24) on a solid or liquid phase sample (4) prior to applying a laser desorption pulse (40). The electric field is changed just prior to or during the desorption laser pulse to promote the desorption of charged species and improve the ionization efficiency of desorbed sample species. After a delay, the electric field may be further changed to optimize focusing and transmission of ions into a mass spectrometer or ion mobility analyzer. Charged species may also be added to the region of the laser desorbed sample plume to promote ion-molecule reactions between the added ions and desorbed neutral sample species, increasing desorbed sample ionization efficiency and/or creating desired product ion species. The cycling of electric field changes is repeated in a timed sequence with one or more desorption laser pulse occurring per electric field change cycle. Embodiments of the invention comprise atmospheric pressure, intermediate pressure and vacuum pressure laser desorption ionization source methods and devices for increasing the analytical flexibility and improving the sensitivity of mass spectrometric analysis.
PCT/US2004/017832 2003-06-07 2004-06-07 Laser desorption ion source WO2004112074A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP04754443.2A EP1639622B1 (en) 2003-06-07 2004-06-07 Laser desorption ion source
CA2527886A CA2527886C (en) 2003-06-07 2004-06-07 Laser desorption ion source

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47657603P 2003-06-07 2003-06-07
US60/476,576 2003-06-07

Publications (2)

Publication Number Publication Date
WO2004112074A2 WO2004112074A2 (en) 2004-12-23
WO2004112074A3 true WO2004112074A3 (en) 2005-10-06

Family

ID=33551616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/017832 WO2004112074A2 (en) 2003-06-07 2004-06-07 Laser desorption ion source

Country Status (3)

Country Link
EP (1) EP1639622B1 (en)
CA (1) CA2527886C (en)
WO (1) WO2004112074A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2595173B1 (en) * 2002-10-29 2016-09-14 Target Discovery, Inc. Method for increasing ionization efficiency in mass spectroscopy
DE102004002729B4 (en) 2004-01-20 2008-11-27 Bruker Daltonik Gmbh Ionization of desorbed analyte molecules at atmospheric pressure
US7737395B2 (en) * 2006-09-20 2010-06-15 Agilent Technologies, Inc. Apparatuses, methods and compositions for ionization of samples and mass calibrants
CZ304648B6 (en) * 2007-12-03 2014-08-20 Masarykova Univerzita Method of preparing samples for atomic spectrometric techniques
US8704169B2 (en) 2011-10-11 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Direct impact ionization (DII) mass spectrometry
EP3265822B1 (en) 2015-03-06 2021-04-28 Micromass UK Limited Tissue analysis by mass spectrometry or ion mobility spectrometry
EP3800657A1 (en) 2015-03-06 2021-04-07 Micromass UK Limited Desorption electrospray ionisation mass spectrometry ("desi-ms") and desorption electroflow focusing ionisation ("deffi-ms") analysis of biological samples on swabs
WO2016142681A1 (en) 2015-03-06 2016-09-15 Micromass Uk Limited Spectrometric analysis of microbes
EP3264989B1 (en) 2015-03-06 2023-12-20 Micromass UK Limited Spectrometric analysis
CN108700590B (en) 2015-03-06 2021-03-02 英国质谱公司 Cell population analysis
GB2552430B (en) 2015-03-06 2022-05-11 Micromass Ltd Collision surface for improved ionisation
EP3265823B1 (en) 2015-03-06 2020-05-06 Micromass UK Limited Ambient ionization mass spectrometry imaging platform for direct mapping from bulk tissue
CA2977906A1 (en) 2015-03-06 2016-09-15 Micromass Uk Limited In vivo endoscopic tissue identification tool
EP4174906A1 (en) 2015-03-06 2023-05-03 Micromass UK Limited Improved ionisation of gaseous samples
GB201517195D0 (en) 2015-09-29 2015-11-11 Micromass Ltd Capacitively coupled reims technique and optically transparent counter electrode
WO2017178833A1 (en) 2016-04-14 2017-10-19 Micromass Uk Limited Spectrometric analysis of plants
EP3909067A1 (en) * 2019-01-11 2021-11-17 Helmholtz-Zentrum Potsdam - Deutsches GeoForschungsZentrum GFZ Stiftung des Öffentlichen Rechts des Lands Brandenburg An ion source comprising a structured sample for enhanced ionization
CN111912895A (en) * 2019-05-09 2020-11-10 岛津分析技术研发（上海）有限公司 Mass spectrum imaging device and method under vacuum
US20240079226A1 (en) * 2020-12-21 2024-03-07 Atomic Energy Of Canada Limited / Énergie Atomique Du Canada Limitée Compact laser ion source apparatus and method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468468A (en) * 1981-06-27 1984-08-28 Bayer Aktiengesellschaft Process for the selective analysis of individual trace-like components in gases and liquid
US5625184A (en) * 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
US20020175278A1 (en) * 2001-05-25 2002-11-28 Whitehouse Craig M. Atmospheric and vacuum pressure MALDI ion source

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19608963C2 (en) 1995-03-28 2001-03-22 Bruker Daltonik Gmbh Process for ionizing heavy molecules at atmospheric pressure
US5965884A (en) 1998-06-04 1999-10-12 The Regents Of The University Of California Atmospheric pressure matrix assisted laser desorption
DE19911801C1 (en) * 1999-03-17 2001-01-11 Bruker Daltonik Gmbh Method and device for matrix-assisted laser desorption ionization of substances
US6744041B2 (en) 2000-06-09 2004-06-01 Edward W Sheehan Apparatus and method for focusing ions and charged particles at atmospheric pressure
US6683301B2 (en) 2001-01-29 2004-01-27 Analytica Of Branford, Inc. Charged particle trapping in near-surface potential wells
US6707036B2 (en) 2002-03-21 2004-03-16 Thermo Finnigan Llc Ionization apparatus and method for mass spectrometer system
US6707039B1 (en) 2002-09-19 2004-03-16 Agilent Technologies, Inc. AP-MALDI target illumination device and method for using an AP-MALDI target illumination device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468468A (en) * 1981-06-27 1984-08-28 Bayer Aktiengesellschaft Process for the selective analysis of individual trace-like components in gases and liquid
US5625184A (en) * 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
US20020175278A1 (en) * 2001-05-25 2002-11-28 Whitehouse Craig M. Atmospheric and vacuum pressure MALDI ion source

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1639622A4 *

Also Published As

Publication number Publication date
CA2527886A1 (en) 2004-12-23
WO2004112074A2 (en) 2004-12-23
EP1639622A2 (en) 2006-03-29
CA2527886C (en) 2014-01-14
EP1639622B1 (en) 2016-11-16
EP1639622A4 (en) 2007-11-21

Similar Documents

Publication Publication Date Title
WO2004112074A3 (en) Laser desorption ion source
US7745780B2 (en) Multiplex data acquisition modes for ion mobility-mass spectrometry
WO2004088271A3 (en) Method for increasing ionization efficiency in mass spectroscopy
EP0957508A3 (en) Analysis of biomolecules using time-of-flight mass spectrometry
WO1999040610A3 (en) A tandem time-of-flight mass spectrometer with delayed extraction and method for use
CA2255122A1 (en) Improvements in ms/ms methods for a quadrupole/time of flight tandem mass spectrometer
WO2006014285A3 (en) Pulsed ion source for quadrupole mass spectrometer and method
CA2476597A1 (en) Mass spectrometry method for analysing mixtures of substances
CA2245465A1 (en) Methods and apparatus for tandem mass spectrometry
CA2350041A1 (en) Methods and apparatus for mass spectrometry
CA2163426A1 (en) Method and apparatus for desorption and ionization of analytes
WO2002103746A3 (en) Mass spectrometers and methods of ion separation and detection
WO2006129083A3 (en) Multiple ion injection in mass spectrometry
CA2507834A1 (en) Processes for designing mass separators and ion traps, methods for producing mass separators and ion traps, mass spectrometers, ion traps, and methods for analyzing samples
ATE479895T1 (en) ELECTRON TRANSFER DISSOCIATION FOR BIOPOLYMER SEQUENCE ANALYSIS
CA2852828A1 (en) Adaptive and targeted control of ion populations to improve the effective dynamic range of mass analyser
WO2000017908A3 (en) Charge reduction in electrospray mass spectrometry
WO2003083448A3 (en) Method and system for high-throughput quantitation of small molecules using laser desorption and multiple-reaction-monitoring
WO2007060427A3 (en) Mass spectrometer
WO2006086585A3 (en) Ion sources for mass spectrometry
EP1009015A3 (en) Space change control method for improved ion isolation in ion trap mass spectrometer by dynamically adaptive sampling
WO2007060421A3 (en) Mass spectrometer
WO2002044683A3 (en) Time-of-flight bacteria analyser using metastable source ionization
WO2002058106A3 (en) Method and device for evaluating the state of organisms and natural products and for analysing a gaseous mixture comprising main constituents and secondary constituents
GB2552615A (en) Apparatus for mass analysis of analytes by simultaneous positive and negative ionization

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2527886

Country of ref document: CA

REEP Request for entry into the european phase

Ref document number: 2004754443

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2004754443

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004754443

Country of ref document: EP