WO2005014779A3 - Cyclodextrin affinity purification - Google Patents

Cyclodextrin affinity purification Download PDF

Info

Publication number
WO2005014779A3
WO2005014779A3 PCT/US2004/013841 US2004013841W WO2005014779A3 WO 2005014779 A3 WO2005014779 A3 WO 2005014779A3 US 2004013841 W US2004013841 W US 2004013841W WO 2005014779 A3 WO2005014779 A3 WO 2005014779A3
Authority
WO
WIPO (PCT)
Prior art keywords
species
immobilized
affinity purification
immobilizing
molecular
Prior art date
Application number
PCT/US2004/013841
Other languages
French (fr)
Other versions
WO2005014779A2 (en
WO2005014779A9 (en
Inventor
Joseph John Villafranca
David James Hakes
Karl Frank Johnson
Walter Scott Willet Jr
Chester A Meyers
Original Assignee
Neose Technologies Inc
Joseph John Villafranca
David James Hakes
Karl Frank Johnson
Walter Scott Willet Jr
Chester A Meyers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neose Technologies Inc, Joseph John Villafranca, David James Hakes, Karl Frank Johnson, Walter Scott Willet Jr, Chester A Meyers filed Critical Neose Technologies Inc
Priority to JP2006514275A priority Critical patent/JP2006525808A/en
Priority to US10/555,123 priority patent/US20070105192A1/en
Priority to MXPA05011577A priority patent/MXPA05011577A/en
Priority to AU2004263817A priority patent/AU2004263817A1/en
Priority to CA002524767A priority patent/CA2524767A1/en
Priority to EP04775939A priority patent/EP1629107A2/en
Publication of WO2005014779A2 publication Critical patent/WO2005014779A2/en
Publication of WO2005014779A3 publication Critical patent/WO2005014779A3/en
Publication of WO2005014779A9 publication Critical patent/WO2005014779A9/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins

Abstract

A method of immobilizing a molecular species that include a starch-binding domain is provided. There also is provided a material upon which the molecular species is immobilized, and a material that is capable of immobilizing the species The method includes binding the species to a solid support, e.g., membranes, chromatographic supports and the like. The immobilized species is optionally purified by the method of the invention. Alternatively, the immobilized species is use in another method, such as in a synthesis as a synthetic reagent, or to purify another species that has an affinity for the immobilized species. Exemplary immobilized molecular species include bioactive agents, and biomolecules.
PCT/US2004/013841 2003-05-05 2004-05-05 Cyclodextrin affinity purification WO2005014779A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2006514275A JP2006525808A (en) 2003-05-05 2004-05-05 Affinity purification of cyclodextrin
US10/555,123 US20070105192A1 (en) 2003-05-05 2004-05-05 Cyclodextrin affinity purification
MXPA05011577A MXPA05011577A (en) 2003-05-05 2004-05-05 Cyclodextrin affinity purification.
AU2004263817A AU2004263817A1 (en) 2003-05-05 2004-05-05 Cyclodextrin affinity purification
CA002524767A CA2524767A1 (en) 2003-05-05 2004-05-05 Cyclodextrin affinity purification
EP04775939A EP1629107A2 (en) 2003-05-05 2004-05-05 Cyclodextrin affinity purification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46837403P 2003-05-05 2003-05-05
US60/468,374 2003-05-05

Publications (3)

Publication Number Publication Date
WO2005014779A2 WO2005014779A2 (en) 2005-02-17
WO2005014779A3 true WO2005014779A3 (en) 2005-11-10
WO2005014779A9 WO2005014779A9 (en) 2005-12-08

Family

ID=34135035

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/013841 WO2005014779A2 (en) 2003-05-05 2004-05-05 Cyclodextrin affinity purification

Country Status (7)

Country Link
US (1) US20070105192A1 (en)
EP (1) EP1629107A2 (en)
JP (1) JP2006525808A (en)
AU (1) AU2004263817A1 (en)
CA (1) CA2524767A1 (en)
MX (1) MXPA05011577A (en)
WO (1) WO2005014779A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7662918B2 (en) * 2005-03-03 2010-02-16 Simpson Biotech Co., Ltd. Recombinant protein comprising starch binding domain and use thereof
ATE526412T1 (en) 2005-04-11 2011-10-15 Ca Nat Research Council IDENTIFICATION OF A BETA-1,3-N-ACETYL GALACTOSAMINYL TRANSFERASE (CGTE) FROM CAMPYLOBACTER JEJUNI LIO87
EP2013357A4 (en) 2006-04-19 2012-02-22 Biogenerix Ag Expression of o-glycosylated therapeutic proteins in prokaryotic microorganisms
CN101605809A (en) * 2006-10-31 2009-12-16 善笙生物科技股份有限公司 Starch binding domain and uses thereof
KR100785913B1 (en) * 2006-11-29 2007-12-17 한국과학기술연구원 Polymerized beta-cyclodextrin powder and its preparation method
CA2689481C (en) 2007-04-20 2017-02-14 National Research Council Of Canada Engineered versions of cgtb (.beta.-1,3-galactosyltransferase) enzymes, with enhanced enzymatic properties
ES2582590T3 (en) * 2010-02-16 2016-09-13 Novo Nordisk A/S Purification method
DE102018200602A1 (en) * 2018-01-15 2019-07-18 Technische Universität München Biological synthesis of amino acid chains for the production of peptides and proteins
US20240000959A1 (en) * 2020-11-30 2024-01-04 Cornell University Cancer immunotherapies to promote hyperacute rejection

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HELLMAN J. ET AL: "Expression in E.coli and purification of intracellular proteins by fusion to cyclomaltodextrin glucanotransferase.", J.BIOTECHNOL., vol. 26, no. 2-3, 1992, pages 275 - 288, XP002990024 *
NAKAYAMA A. ET AL: "Two enzyme activities of yeast glycogen debranching anzyme and their catalytic residues.", J.APPL.GLYCOSCI., vol. 49, no. 2, 2002, pages 181 - 190, XP008051219 *
TAKADA M. ET AL: "Biochemical and genetic analyses of a novel gamma-cyclodextrin glucanotransferase from an alkalophilic B.clarkii 7364.", J.BIOCHEM., vol. 133, no. 3, March 2003 (2003-03-01), pages 317 - 324, XP009038339 *
TARDIOLI P. ET AL: "Production of cyclodextins in a fluidized.bed reactor using cyclodextrin-glycosyl-transferase.", APPL.BIOCHEM.BIOTECHNOL., vol. 84-86, 2000, pages 84 - 86, XP008051820 *

Also Published As

Publication number Publication date
MXPA05011577A (en) 2005-12-15
WO2005014779A2 (en) 2005-02-17
WO2005014779A9 (en) 2005-12-08
EP1629107A2 (en) 2006-03-01
JP2006525808A (en) 2006-11-16
AU2004263817A1 (en) 2005-02-17
CA2524767A1 (en) 2005-02-17
US20070105192A1 (en) 2007-05-10

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