WO2005017153A3 - Methods for synthesis of defined polynucleotides - Google Patents

Methods for synthesis of defined polynucleotides Download PDF

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Publication number
WO2005017153A3
WO2005017153A3 PCT/US2004/025104 US2004025104W WO2005017153A3 WO 2005017153 A3 WO2005017153 A3 WO 2005017153A3 US 2004025104 W US2004025104 W US 2004025104W WO 2005017153 A3 WO2005017153 A3 WO 2005017153A3
Authority
WO
WIPO (PCT)
Prior art keywords
synthesis
producing
methods
amino acids
selected subset
Prior art date
Application number
PCT/US2004/025104
Other languages
French (fr)
Other versions
WO2005017153A2 (en
Inventor
Jeffrey S Nelson
John T Mulligan
John C Tabone
Original Assignee
Blue Heron Biotechnology Inc
Jeffrey S Nelson
John T Mulligan
John C Tabone
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Blue Heron Biotechnology Inc, Jeffrey S Nelson, John T Mulligan, John C Tabone filed Critical Blue Heron Biotechnology Inc
Publication of WO2005017153A2 publication Critical patent/WO2005017153A2/en
Publication of WO2005017153A3 publication Critical patent/WO2005017153A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR

Abstract

Disclosed is a significantly improved synthetic method of producing a set of mutagenized progeny polynucleotides which contain at least one substituted codon encoding for each of the 20 naturally encoded amino acids or any selected subset thereof. This in turn, similarly provides a method for producing from a parental template polypeptide, a set of mutagenized progeny polypeptides in which all 20 naturally encoded amino acids is represented at each original amino acid position or any selected subset thereof. The methods described herein enable the synthesis of defined, complex mixtures of oligonucleotides, in instances where the incorporation of degenerate bases is impractical. These oligonucleotide mixtures are useful for a variety of applications such as recombination methods, site-saturation mutagenesis, or the like.
PCT/US2004/025104 2003-08-04 2004-08-03 Methods for synthesis of defined polynucleotides WO2005017153A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49269403P 2003-08-04 2003-08-04
US60/492,694 2003-08-04

Publications (2)

Publication Number Publication Date
WO2005017153A2 WO2005017153A2 (en) 2005-02-24
WO2005017153A3 true WO2005017153A3 (en) 2005-06-09

Family

ID=34193143

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/025104 WO2005017153A2 (en) 2003-08-04 2004-08-03 Methods for synthesis of defined polynucleotides

Country Status (2)

Country Link
US (1) US20050069928A1 (en)
WO (1) WO2005017153A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
US20060194214A1 (en) * 2005-02-28 2006-08-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
AU2005295351A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
EP3360963B1 (en) 2010-11-12 2019-11-06 Gen9, Inc. Methods and devices for nucleic acids synthesis
AU2012300401B2 (en) 2011-08-26 2018-02-08 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
CN104685116A (en) 2012-06-25 2015-06-03 Gen9股份有限公司 Methods for nucleic acid assembly and high throughput sequencing

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001023401A2 (en) * 1999-09-28 2001-04-05 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic sequence recombination
WO2002050094A2 (en) * 2000-12-20 2002-06-27 Birkbeck College Nucleic acid synthesis by primer extension on successive templates

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4336911C2 (en) * 1993-10-28 2002-11-21 Biotechnolog Forschung Gmbh Primer production process and template bank therefor
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6171802B1 (en) * 1998-06-10 2001-01-09 Kent State University Detection and amplification of ligands
US5998175A (en) * 1998-07-24 1999-12-07 Lumigen, Inc. Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001023401A2 (en) * 1999-09-28 2001-04-05 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic sequence recombination
WO2002050094A2 (en) * 2000-12-20 2002-06-27 Birkbeck College Nucleic acid synthesis by primer extension on successive templates

Also Published As

Publication number Publication date
US20050069928A1 (en) 2005-03-31
WO2005017153A2 (en) 2005-02-24

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