WO2005026686A3 - Multiplexed analytical platform - Google Patents
Multiplexed analytical platform Download PDFInfo
- Publication number
- WO2005026686A3 WO2005026686A3 PCT/US2004/028728 US2004028728W WO2005026686A3 WO 2005026686 A3 WO2005026686 A3 WO 2005026686A3 US 2004028728 W US2004028728 W US 2004028728W WO 2005026686 A3 WO2005026686 A3 WO 2005026686A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tags
- hybridization
- constructed
- readout
- words
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Abstract
The invention provides a system and reagents for carrying out multiplexed analytical measurements using the hybridization of oligonucleotide tags to addressable solid phase supports to obtain simultaneous readouts from hundreds to tens of thousands of reactions. In one aspect of the invention, analyte interaction moieties, such as oligonucleotide probes, are produced that are each labeled with a unique synthesis tag constructed from a set of two-nucleotide 'words.' During or after an assay, the synthesis tags are converted to hybridization tags that are used in the readout step. Hybridization tags are constructed to maximize discrimination in the readout. Hybridization tags of the invention are preferably constructed from oligonucleotide 'words' selected from a minimally cross-hybridizing set using combinatorial synthesis techniques. hi a further aspect, discrimination in the readout is enhanced by employing hybridization tags that are constructed with words having the 'comma-less' property and by minimizing or eliminating extraneous nucleic acids associated with the hybridization tag during a readout step.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US50127303P | 2003-09-09 | 2003-09-09 | |
US60/501,273 | 2003-09-09 | ||
US54388704P | 2004-02-12 | 2004-02-12 | |
US60/543,887 | 2004-02-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005026686A2 WO2005026686A2 (en) | 2005-03-24 |
WO2005026686A3 true WO2005026686A3 (en) | 2006-02-09 |
Family
ID=34316472
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2004/028728 WO2005026686A2 (en) | 2003-09-09 | 2004-09-02 | Multiplexed analytical platform |
Country Status (2)
Country | Link |
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US (1) | US7365179B2 (en) |
WO (1) | WO2005026686A2 (en) |
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Also Published As
Publication number | Publication date |
---|---|
US7365179B2 (en) | 2008-04-29 |
WO2005026686A2 (en) | 2005-03-24 |
US20050059065A1 (en) | 2005-03-17 |
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