WO2005027945A1

WO2005027945A1 - Mahonia aquifolium extract, extraction process and pharmaceutical composition containing the same

Info

Publication number: WO2005027945A1
Application number: PCT/CA2004/001726
Authority: WO
Inventors: Robert Mills; Rajiv Mathur; Nadya Lawrence
Original assignee: Prime Pharmaceutical Corporation
Priority date: 2003-09-25
Filing date: 2004-09-22
Publication date: 2005-03-31
Also published as: US20070148226A1; US20050069576A1; IL174206A; CA2538867A1; JP2007506685A; KR20060069514A; AU2004273556A1; IL174206A0; US7621740B2; DE602004030131D1; EP1663154A1; ZA200602143B; EP1663154A4; RU2388482C2; CN1856318A; EP1663154B1; MXPA06003146A; AU2004273556B2; BRPI0414786A; NZ545853A

Abstract

The present invention provides a skin treatment composition comprising a Mahonia aquifolium extract in a liposome delivery system. The Mahonia aquifolium extract is present in the skin treatment composition in a range of from 5% to 20% by weight of the total composition. The skin treatment composition is particularly effective in the treatment of psoriasis, eczema and other dry skin conditions. The invention further provides a process for obtaining an extract of Mahonia aquifolium.

Description

Mahonia Acpiifolium Extract, Extraction Process and Pharmaceutical Composition Containing the Same

Technical Field

The present invention is directed to a Mahonia aquifolium extract, a process for obtaining a concentrated form of the extract using a new extraction process and a pharmaceutical composition containing- the extract. The pharmaceutical composition is a particularly effective treatment for psoriasis, eczema, dermatitis and other dry skin conditions.

Background Art

Psoriasis is a chronic skin disease that is characterized by scaling and inflammation of the skiin. The scaling occurs when the cells in the outer layer of the skin reproduce faster than normal and pile up on the skin's surface. Psoriasis affects about 1.5% to 2% of the North American population. It occurs in all ace groups and affects men and women equally. People affected by psoriasis suffer from discomfort, restricted joint motion and emotional distress. About 10% of people suffering from psoriasis have joint inflammation that produces symptoms similar to arthritis .

When psoriasis develops, patches of skin thicken, redden and become covered with silvery scales. These patches are generally referred to as plaques. The plaques are usually itchy and can burn. Psoriasis most often occurs on the elbows, knees, scalp, lower back, face, palms and soles of the feet.

A variety of treatments and methods have been used over the years including the topical application of corticosteroids, calcipotriene, coal tar, etc. Bath solutions and general moisturizers have been utilized by some patients. Sunlight and ultraviolet light treatments have also been used. In some instances systemic treatment using prescribed medicines taken internally are needed. Drugs taken internally include retinoids, methotrexate, hydroxyurea and antibiotics.

Each of these treatments has its benefits and drawbacks. In many instances, patients develop a tolerance to the treatment and the treatment becomes much less effective. The inventors have sought a treatment suitable for a variety of patients that overcomes the drawbacks noted above .

Mahonia aquifolium was originally found in the Pacific Northwest and British Columbia where it has been used for the treatment of psoriasis and eczema by Native

North Americans for many centuries. It is an evergreen shrub that belongs to the Berberidaceae family.

The root and bark of the Mahonia aquifolium plant are known to contain isoquinoline alkaloids that include berberine, palmatine, berbamine, oxyacanthine, jatrorrhizine, bervulcine, magnoflorine and columbamine. These alkaloids are thought to be the active constituents of the plants as many of them have shown strong in vi tro anti-microbial and anti-fungal activity.

Mahonia aquifolium may have several mechanisms of action in the treatment and management of psoriasis and other inflammatory conditions. Berberine and related alkaloids as noted above reversibly intercalate in DNA hindering replication and transcription. Mahonia extract contains isoquinoline alkaloids as outlined above. Isoquinoline has been identified as a component of coal tar which causes interfollicular regions of parakeratotic stratum corneum in mouse tail epidermis to become orthokeratotic, with concomitant production of a granular layer. In this respect, isoquinoline behaves similarly to coal tar and the isoquinolines may contribute to the anti-psoriatic activity of coal tar. Alkaloids isolated from the extract demonstrate anti- proliferation activity on keratinocytes . Hyper proliferation of keratinocytes is a major symptom of psoriasis and so controlling this activity will assist in the treatment of psoriasis. Lipoxygenase inhibition is strongly correlated with the lipid antioxidant effect of the protoberberine alkaloids, perhaps by reducing lipid hydroperoxide substrate accumulation. Mahonia is a moderate inhibitor of LTB-4 (a leukotriene which is believed to mediate inflammation) and 5-hydroxy- eicosatetraenoic acid (5-HETE) with an IC-50 in the same order of magnitude as anthralin. IC-50 refers to the concentration at which 50% inhibition occurs. Mahonia aquifolium extract is also a very potent inhibitor of lipid peroxidation. Individual alkaloids isolated from the extract demonstrate anti-inflammatory activity by inhibiting the action of lipoxygenase. Oxidation products resulting from the action of lipoxygenase are known to be mediators of inflammation in other biological systems. The inhibitory effect of Mahonia aquifolium on lipid peroxidation contrasts with anthralin, which slightly stimulates lipid peroxidation. This difference may explain why Mahonia aquifolium extract reduces irritation.

Disclosure of Invention

The Mahonia aquifolium extract of the present invention is obtained in a highly concentrated form using an extraction process as detailed below. The regents used in the extraction process are water, alcohol and crude dried Mahonia aquifolium.

The crude dried Mahonia aquifolium is obtained from dried bark and twigs of plants from the Mahonia aquifolium (Berberidaceae) family. Such plants include plants of the barberry family, for example, Oregon hollygrape .

The reagents are loaded into a stainless steel reactor vessel. The vessel is clamped shut. Pressure of 3 to 6 psi (volume dependent) is applied to the reactor vessel and the reagent mixture is heated to a temperature not higher than 50²C, preferably about 40^SC, while mixing the contents with an internal counter-rotating agitating mixer. When the mixture reaches 40 ^ΞC an internal grinding mixer is engaged and the mixture is processed at a speed of about 3000rpm (revolutions per minute) in combination with the internal counter-rotating mixer for three hours. The mixture is left under pressure of 3 to 6 psi in the reactor and is allowed to cool for not less than 24 hours.

After the 24 hours has elapsed the internal grinding mixer is again engaged and the mixture is processed at a speed of about 3000rpm in combination with the internal counter-rotating mixer for three hours . The mixture is left under pressure of about 3 to 6 psi in the reactor for not less than 24 hours .

After the second 24 hour period, the internal grinding mixer is again engaged and the mixture is processed at a speed of about 3000rpm in combination with the internal counter-rotating mixer for three hours . The mixture is then left under pressure of about 3 to 6 psi in the reactor for a minimum of 24 hours.

After the third 24 hour period, the pressure is released in the reactor and the reagent mixture is filtered through a coarse mesh filter and then through a 5 micron filter. The mixture is then placed under vacuum and heated to a minimum of 40²C but not more than 50²C while mixing to reduce and remove the solvents until the mixture is approximately 6% of its original volume. The resultant product is re-filtered through a 1-micron filter.

This extraction process yields a finished Mahonia aquifolium extract with a concentration of approximately 1.5mg/ml berberine alkaloid. A typical alcohol based extraction process will yield a finished extract with a concentration of approximately 0.09mg/ml berberine alkaloid.

The product obtained from this extraction process is then utilized to prepare a pharmaceutical composition suitable for use in the treatment of psoriasis and other skin ailments and diseases.

The pharmaceutical composition is prepared for use by combining the Mahonia aquifolium extract in a liposome delivery system.

Liposomes are microscopic spherical vesicles that form when phospholipids are hydrated. Recently, liposomes have been utilized and evaluated as delivery systems for drugs, vitamins and cosmetic materials. They can be custom designed for almost any use by varying the lipid content, size, surface charge and method of preparation. As the liposome wall is very similar, physiologically, to the material of cell membranes, they are particularly suitable for the delivery of products for application to the skin. When a pharmaceutical or cosmetic containing liposomes is applied to the skin, the liposomes are deposited on the skin and begin to merge with the cellular membranes . The liposomes release the active material into the cells and as a result the delivery of the active material is very specific. The liposome delivery system can be designed to release the active materials under a variety of conditions, for example, slow release, fast release, bilayer compositions, temperature dependent release, pH-dependent release, etc.

A preferred liposome delivery system for use in the pharmaceutical composition of the present invention is the Novasome® technology. Novasome lipid vesicles are comprised of molecules that possess both hydrophilic and hydrophobic properties (i.e. amphiphiles) . The Novasome vesicles are made up of one or more lipid membranes or bilayers (generally 5 to 7) that surround a larger core. Each membrane is composed of amphiphiles in a bilayer array.

The core accounts for most of the vesicle volume, providing a high carrying capacity for water-soluble and water-immiscible substances. The Novasome bilayers are not in perfect array and contain vacancies (channels) through which encapsulated components can travel . Encapsulated components travel within and between each bilayer via a series of random jumps which causes lateral movement of the vacancies in the bilayer. An active ingredient is contained in the core and can pass from the core through the bilayer. A sustained release mechanism is provided by the structure of the Novasome vesicles so a continuous and controlled release of the active ingredient can be achieved. As the active ingredient is entrapped within the core in a more protected manner prior to use the storage stability and the stability of the active ingredient is improved. This provides for more efficient and effective delivery of the active ingredient.

The pharmaceutical composition is prepared by combining Mahonia aquifolium extract in a concentration of approximately 5% to 20% by weight of the total composition in a liposome delivery system. The liposome delivery system detailed just above is one preferred delivery system. Preferred compositions have a concentration of Mahonia aquifolium of between 5% and 10% by weight of the total composition. This will provide the composition with a minimum of 0.712% berberine.

The remainder of the composition comprises typical pharmaceutically acceptable components, for example, diluents, adjuvants, etc.

The composition is utilized in a topical application form and is applied directly to the affected area. The composition can be in the form of a lotion or cream. Three typical formulations are detailed below. The percent by weight noted of each component is a percent by weight of the total composition. The Mahonia aquifolium extract is in the liposome delivery system.

Lotion

Component percent by weight

DI water 51.04

Mahonia extract 10.00 transcutol 8.00 glyceryl dilaurate 8.00 glyceryl monostearate 4.50

96% glycerin 3.41 diisopropyl adipate 3.00 eyelomethicone 2.50 dimethicone 2.00 octyl palmitate 1.40

Cχ₂_i5 alcohol lactate 1.00 chamomile extract 1.00 stearoxydimethicone 0.70 cholesterol 0.50

Polysorbate 80™ 0.50 cetearyl glucoside 0.50 cetyl alcohol 0.50 green tea extract 0.50 phenoxyethanol 0.40

Methylparaben™ 0.20 xanthan gum 0.15 disodium EDTA 0.10 vitamin E acetate 0.10

Cream

Component percent by weight DI water 44.95

Mahonia extract 10.00 transcutol 10.00 glyceryl dilaurate 8.00 glyceryl monostearate 4.50 96% glycerin 3.41 cyclomethicone 3.00 dimethicone 2.50 cetearyl glucoside 1.50

PEG-150 monstearate 1.50 diisopropyl adipate 1.50 stearoxydimethicone 1.00

C12-15 alcohol lactate 1.00 ethyl oleate 1.00 diisopropyl sebacate 1.00 cetyl alcohol 1.00 chamomile extract 1.00

Sepigel 305™ 1.00 green tea extract 0.50 cholesterol 0.50 phenoxyethanol 0.40 xanthan gum 0.30

Methylparaben 0.20 disodium EDTA 0.10 alpha toccopherol acetate 0.10 citric acid 0.04

Scalp Therapy Composition

Component percent by weight DI water 67.34

Mahonia extract 10.00 glyceryl monostearate 4.50 chamomile extract 3.00 propylene glycol 2.50 avocado unsaponifiable 2.00

Quaterium 22™ 2.00 cyclomethicone 1.40 behentrimonium methosulfate 1.00 cetearyl alcohol 1.00 aloe vera powder 1.00 green tea extract 1.00 wheat germ oil 1.00 stearyl alcohol 0.70 triethanolamine 0.66 Quaterium 26™ 0.50

Methylparaben 0.20

DMDM hydantoin 0.20

In clinical studies and trials, as detailed below, the beneficial effects of the composition of the present invention were noted.

In one trial psoriatic patients having mild to moderate psoriasis applied the composition of the present invention to one half of the affected area. To the other side of the affected area the patients applied a plain vehicle cream (the cream utilized to prepare the composition of the present invention) . The compositions were applied twice a day for four weeks . All eleven patients were compliant and finished the four week course of the study. Nine patients improved, one remained the same throughout the treatment but was less pruritic and one improved on the plain vehicle cream side only. Of the nine patients that improved, one patient cleared on both sides, one patient improved on both sides equally, one patient improved slightly on the side utilizing the composition of the present invention and six patients were definitely improved on the side utilizing the composition of the present invention.

In another study low concentrations of Mahonia aquifolium extract in a cream base demonstrated that the preparation was safe and well tolerated when applied to plaques of psoriasis. A second study was initiated to examine the safety, tolerability and efficacy of higher concentrations of Mahonia aquifolium extract in 33 patients with a variety of types of psoriasis and 8 patients with eczema. The study was an observational study.

The patient population included a wide range of age groups and included both sexes . Treatments prior to the start of the application of Mahonia aquifolium extract ranged from no treatment to topical corticosteroids, Dovonex™, ultraviolet light and methotrexate .

Mahonia extract and vehicle treated lesions were photographed prior to treatment and throughout the study. The target lesions were scored for scaling, thickness and redness. In almost all cases scaling -was the first symptom to improve. In many cases improvement was noted after the first week of treatment. The thickness of the plaques generally declined substantially over 2 to 4 weeks. Redness improved most gradually. This is common for most psoriasis treatments .

In some cases the methotrexate and/or ultraviolet light treatment continued when the application of Mahonia extract started. The Mahonia extract did not cause any adverse interactions with these treatments and there was no evidence of photosensitivity or phototoxicity . Mahonia cream may be a complementary treatment. There was no clinical evidence that the creams being tested blocked or interfered with ultraviolet light.

The Mahonia cream was enthusiastically accepted by all the patients and compliance with and adherence to th.e experimental protocol was good. In all cases of both, psoriasis and eczema the side treated with Mahonia cream did as well as or better than the side treated with the vehicle cream alone. Industrial Applicability

The Mahonia aquifolium extract obtained loy the process of the present invention is suitable for use in a pharmaceutical composition that can be used as an effective treatment for psoriasis, eczema., dermatitis and other dry skin conditions .

Claims

Claims :

1. A skin treatment composition comprising an extract of Mahonia aquifolium in a liposome delivery system.

2. A skin treatment composition as claimed in claim 1 wherein the extract of Mahonia aquifolium is present in a concentration of from 5% t 20% by weight of the total composition.

3. A skin treatment composition as claimed in claim 1 wherein the extract of Mahonia aquifolium is present in a concentration of 10% by weight of the total composition.

4. A process for obtaining an extract of Mahonia aquifolium comprising the steps of: (a) applying heat and pressure to a mixture of water, alcohol and crude dried Mahonia aquifolium; (b) stirring the mixture; (c) processing the mixture at a speed of about 3000rpm for about three hours; (d) allowing the mixture to cool under pressure for at least 24 hours; (e) repeating the last two steps at least twice more; (f) releasing the pressure; (g) placing the mixture under vacuum with heating and mixing to reduce and remove the alcohol and water to obtain a mixture of approximately 6% by volume; and. (h) filtering the resulting mixture to obtain the extract of Mahoni a aquifolium.

5. The method of claim 4 wherein in step (a) the mixture is heated to a temperature of not higher than 50 ^aC.

6. The method of claim 4 wherein in step (a) the mixture is heated to a temperature of 40²C.

7. The method of claim 4 wherein the pressure is in a range of about 3 to 6 psi.

8. An extract of Mahonia aquifolium obtained by the process of claim 4.

9. Method of treating psoriasis, eczema and other dry skin conditions by applying the skin treatment composition of claim 1.