WO2005040399A3 - Methods for quantitative determination of methylation density in a dna locus - Google Patents
Methods for quantitative determination of methylation density in a dna locus Download PDFInfo
- Publication number
- WO2005040399A3 WO2005040399A3 PCT/US2004/035177 US2004035177W WO2005040399A3 WO 2005040399 A3 WO2005040399 A3 WO 2005040399A3 US 2004035177 W US2004035177 W US 2004035177W WO 2005040399 A3 WO2005040399 A3 WO 2005040399A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- methylation density
- quantitative determination
- dna locus
- locus
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006536855A JP4887154B2 (en) | 2003-10-21 | 2004-10-21 | Method for quantitative measurement of methylation density at DNA loci |
CA2542526A CA2542526C (en) | 2003-10-21 | 2004-10-21 | Methods for quantitative determination of methylation density in a dna locus |
AU2004284456A AU2004284456B2 (en) | 2003-10-21 | 2004-10-21 | Methods for quantitative determination of methylation density in a DNA locus |
EP04796212A EP1689887B1 (en) | 2003-10-21 | 2004-10-21 | Methods for quantitative determination of methylation density in a dna locus |
AT04796212T ATE550438T1 (en) | 2003-10-21 | 2004-10-21 | METHOD FOR QUANTITATIVELY DETERMINING THE METHYLATION DENSITY IN A DNA LOCUS |
ES04796212T ES2382780T3 (en) | 2003-10-21 | 2004-10-21 | Procedures for quantitative determination of methylation density in a DNA locus |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51342603P | 2003-10-21 | 2003-10-21 | |
US60/513,426 | 2003-10-21 | ||
US56172104P | 2004-04-12 | 2004-04-12 | |
US56156304P | 2004-04-12 | 2004-04-12 | |
US60/561,721 | 2004-04-12 | ||
US60/561,563 | 2004-04-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005040399A2 WO2005040399A2 (en) | 2005-05-06 |
WO2005040399A3 true WO2005040399A3 (en) | 2005-07-21 |
Family
ID=34527938
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2004/035177 WO2005040399A2 (en) | 2003-10-21 | 2004-10-21 | Methods for quantitative determination of methylation density in a dna locus |
PCT/US2004/035012 WO2005042704A2 (en) | 2003-10-21 | 2004-10-21 | Differential enzymatic fragmentation |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2004/035012 WO2005042704A2 (en) | 2003-10-21 | 2004-10-21 | Differential enzymatic fragmentation |
Country Status (8)
Country | Link |
---|---|
US (4) | US7901880B2 (en) |
EP (4) | EP2339024B1 (en) |
JP (2) | JP4887154B2 (en) |
AT (2) | ATE553218T1 (en) |
AU (2) | AU2004284456B2 (en) |
CA (2) | CA2542542C (en) |
ES (2) | ES2383970T3 (en) |
WO (2) | WO2005040399A2 (en) |
Cited By (3)
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US8962247B2 (en) | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
US9249456B2 (en) | 2004-03-26 | 2016-02-02 | Agena Bioscience, Inc. | Base specific cleavage of methylation-specific amplification products in combination with mass analysis |
US9394565B2 (en) | 2003-09-05 | 2016-07-19 | Agena Bioscience, Inc. | Allele-specific sequence variation analysis |
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---|---|---|---|---|
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WO2005085477A1 (en) * | 2004-03-02 | 2005-09-15 | Orion Genomics Llc | Differential enzymatic fragmentation by whole genome amplification |
US7608394B2 (en) * | 2004-03-26 | 2009-10-27 | Sequenom, Inc. | Methods and compositions for phenotype identification based on nucleic acid methylation |
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AU2005284980A1 (en) * | 2004-09-10 | 2006-03-23 | Sequenom, Inc. | Methods for long-range sequence analysis of nucleic acids |
US7820385B2 (en) * | 2006-03-22 | 2010-10-26 | The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention | Method for retaining methylation pattern in globally amplified DNA |
US7754428B2 (en) * | 2006-05-03 | 2010-07-13 | The Chinese University Of Hong Kong | Fetal methylation markers |
WO2008008501A2 (en) * | 2006-07-14 | 2008-01-17 | University Of Georgia Research Foundation, Inc. | Reverse restriction fragment length polymorphism assay and uses thereof |
TWI335354B (en) | 2006-09-27 | 2011-01-01 | Univ Hong Kong Chinese | Methods for the detection of the degree of the methylation of a target dna and kits |
WO2008047234A1 (en) | 2006-10-18 | 2008-04-24 | Epigenomics Ag | A molecule for providing a standard for the quantitative analysis of the methylation status of a nucleic acid |
JP2008136404A (en) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Method for confirming amount of dna after conversion treatment of non-methylated cytosine in dna methylation detection |
US8206927B2 (en) * | 2007-01-23 | 2012-06-26 | Sequenom, Inc. | Method for accurate assessment of DNA quality after bisulfite treatment |
EP2150625B1 (en) * | 2007-04-19 | 2013-03-20 | Molecular Detection Inc. | Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria |
US20090181391A1 (en) * | 2007-11-01 | 2009-07-16 | Zymo Research Corporation | Methods for analysis of dna methylation percentage |
EP2271772B1 (en) * | 2008-03-11 | 2014-07-16 | Sequenom, Inc. | Nucleic acid-based tests for prenatal gender determination |
US20090253121A1 (en) * | 2008-04-04 | 2009-10-08 | Micah Halpern | Method for amt-rflp dna fingerprinting |
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US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
US20120088237A1 (en) * | 2009-03-09 | 2012-04-12 | New England Biolabs, Inc. | Engineering a Novel Methylation-Specific Restriction Endonuclease |
WO2011001274A2 (en) * | 2009-07-02 | 2011-01-06 | Nucleix | Methods for distinguishing between natural and artificial dna samples |
JP2013511991A (en) * | 2009-11-25 | 2013-04-11 | クアンタライフ, インコーポレイテッド | Methods and compositions for detecting genetic material |
US9783850B2 (en) * | 2010-02-19 | 2017-10-10 | Nucleix | Identification of source of DNA samples |
US9752187B2 (en) | 2009-12-11 | 2017-09-05 | Nucleix | Categorization of DNA samples |
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GB201003036D0 (en) | 2010-02-23 | 2010-04-07 | Fermentas Uab | Restriction endonucleases and their applications |
US20130065233A1 (en) * | 2010-03-03 | 2013-03-14 | Zymo Research Corporation | Detection of dna methylation |
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WO2012031021A2 (en) * | 2010-09-01 | 2012-03-08 | Orion Genomics Llc | Improved detection of nucleic acid sequences adjacent to repeated sequences |
JP2014506465A (en) | 2011-02-09 | 2014-03-17 | バイオ−ラド ラボラトリーズ,インコーポレイティド | Nucleic acid analysis |
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WO2013123481A1 (en) | 2012-02-16 | 2013-08-22 | Cornell University | Methods and kit for characterizing the modified base status of a transcriptome |
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US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
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WO2014168711A1 (en) | 2013-03-13 | 2014-10-16 | Sequenom, Inc. | Primers for dna methylation analysis |
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US9984201B2 (en) | 2015-01-18 | 2018-05-29 | Youhealth Biotech, Limited | Method and system for determining cancer status |
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US20200131566A1 (en) * | 2018-10-31 | 2020-04-30 | Guardant Health, Inc. | Methods, compositions and systems for calibrating epigenetic partitioning assays |
IL265451B (en) * | 2019-03-18 | 2020-01-30 | Frumkin Dan | Methods and systems for detecting methylation changes in dna samples |
CA3134936A1 (en) | 2019-03-26 | 2020-10-01 | Dermtech, Inc. | Novel gene classifiers and uses thereof in skin cancers |
US20220325316A1 (en) * | 2019-08-09 | 2022-10-13 | Battelle Memorial Institute | Compositions and methods for detecting methylated dna |
US11427874B1 (en) * | 2019-08-26 | 2022-08-30 | Epi One Inc. | Methods and systems for detection of prostate cancer by DNA methylation analysis |
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WO2024044578A1 (en) | 2022-08-22 | 2024-02-29 | University Of Virginia | Dna methylation biomarkers of premenstrual dysphoric disorder and perimenopausal depression |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5405760A (en) * | 1992-04-30 | 1995-04-11 | New England Biolabs, Inc. | Process for producing recombinant McrBC endonuclease and cleavage of methylated DNA |
Family Cites Families (59)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4376110A (en) | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4517288A (en) | 1981-01-23 | 1985-05-14 | American Hospital Supply Corp. | Solid phase system for ligand assay |
US4732847A (en) | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
ZA853756B (en) | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
CA1291031C (en) | 1985-12-23 | 1991-10-22 | Nikolaas C.J. De Jaeger | Method for the detection of specific binding agents and their correspondingbindable substances |
DE3803275A1 (en) | 1988-02-04 | 1989-08-17 | Dornier Medizintechnik | PIEZOELECTRIC SHOCK WAVE SOURCE |
US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US6033854A (en) | 1991-12-16 | 2000-03-07 | Biotronics Corporation | Quantitative PCR using blocking oligonucleotides |
WO1995006137A1 (en) | 1993-08-27 | 1995-03-02 | Australian Red Cross Society | Detection of genes |
US5552277A (en) | 1994-07-19 | 1996-09-03 | The Johns Hopkins University School Of Medicine | Genetic diagnosis of prostate cancer |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
US5871917A (en) | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
US6017704A (en) | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5912147A (en) * | 1996-10-22 | 1999-06-15 | Health Research, Inc. | Rapid means of quantitating genomic instability |
US6686151B1 (en) | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
US7700324B1 (en) | 1998-11-03 | 2010-04-20 | The Johns Hopkins University School Of Medicine | Methylated CpG island amplification (MCA) |
WO2000042222A2 (en) * | 1999-01-15 | 2000-07-20 | Gene Logic Inc. | Immobilized nucleic acid hybridization reagent and method |
GB9901072D0 (en) | 1999-01-19 | 1999-03-10 | Imp Cancer Res Tech | Methods for detecting changes to a macromolecular component of a cell |
US6605432B1 (en) | 1999-02-05 | 2003-08-12 | Curators Of The University Of Missouri | High-throughput methods for detecting DNA methylation |
US20010046669A1 (en) | 1999-02-24 | 2001-11-29 | Mccobmie William R. | Genetically filtered shotgun sequencing of complex eukaryotic genomes |
US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
US6180349B1 (en) | 1999-05-18 | 2001-01-30 | The Regents Of The University Of California | Quantitative PCR method to enumerate DNA copy number |
JP2003527087A (en) | 1999-08-13 | 2003-09-16 | イェール・ユニバーシティ | Binary coded array tags |
WO2001068807A2 (en) | 2000-03-16 | 2001-09-20 | Fred Hutchinson Cancer Research Center | Identification of in vivo dna binding loci of chromatin proteins using a tethered nucleotide modification enzyme |
US20040033490A1 (en) | 2000-03-31 | 2004-02-19 | Laird Peter W. | Epigenetic sequences for esophageal adenocarcinoma |
EP1276865B1 (en) * | 2000-04-28 | 2014-05-21 | Sangamo BioSciences, Inc. | Methods for binding an exogenous molecule to cellular chromatin |
US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
EP1339868A2 (en) | 2000-06-23 | 2003-09-03 | The University of Chicago | Methods for isolating centromere dna |
US7300751B2 (en) | 2000-06-30 | 2007-11-27 | Syngenta Participations Ag | Method for identification of genetic markers |
AU2001280875A1 (en) | 2000-07-28 | 2002-02-13 | The Johns Hopkins University | Serial analysis of transcript expression using long tags |
CA2421102A1 (en) | 2000-08-25 | 2002-03-07 | Lovelace Respiratory Research Institute | Nested methylation-specific polymerase chain reaction cancer detection method |
ES2299520T3 (en) | 2000-09-01 | 2008-06-01 | Epigenomics Ag | PROCEDURE FOR THE DETERMINATION OF THE DEGREE OF METHYLATION OF CERTAIN CITOSINES IN GENOMIC DNA IN THE SEQUENTIAL CONTEXT 5'-CPG-3 '. |
CA2428245A1 (en) * | 2000-11-08 | 2002-05-16 | University Of Southern California | A new assay for the detection and quantitation of hemimethylation |
US6756200B2 (en) | 2001-01-26 | 2004-06-29 | The Johns Hopkins University School Of Medicine | Aberrantly methylated genes as markers of breast malignancy |
US6777187B2 (en) | 2001-05-02 | 2004-08-17 | Rubicon Genomics, Inc. | Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates |
DE10128508A1 (en) | 2001-06-14 | 2003-02-06 | Epigenomics Ag | Methods and nucleic acids for the differentiation of prostate tumors |
WO2003002752A2 (en) | 2001-06-29 | 2003-01-09 | Rubicon Genomics Inc. | Methods of using nick translate libraries for snp analysis |
WO2003023065A1 (en) | 2001-09-06 | 2003-03-20 | Syngenta Participations Ag | Dna methylation patterns |
EP1438428A4 (en) | 2001-09-14 | 2005-11-16 | Univ Queensland | Detection of dna methylation |
US20060183115A1 (en) | 2001-09-26 | 2006-08-17 | Epigenx Pharmaceuticals | Assays for dna methylation changes |
US20030099997A1 (en) * | 2001-10-24 | 2003-05-29 | Bestor Timothy H. | Method for gene identification based on differential DNA methylation |
DE10154318A1 (en) | 2001-10-26 | 2003-05-15 | Epigenomics Ag | Method for analyzing genomic methylation patterns |
EP1451365A4 (en) | 2001-11-13 | 2006-09-13 | Rubicon Genomics Inc | Dna amplification and sequencing using dna molecules generated by random fragmentation |
US6960436B2 (en) | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
WO2003076666A1 (en) | 2002-03-08 | 2003-09-18 | Naxcor | Compositions and methods for detecting nucleic acid methylation |
US20030175828A1 (en) | 2002-03-15 | 2003-09-18 | Lazar James G. | Signal amplification by Hybrid Capture |
AU2003220291A1 (en) | 2002-03-15 | 2003-09-29 | Epigenomics Ag | Discovery and diagnostic methods using 5-methylcytosine dna glycosylase |
JP4384603B2 (en) * | 2002-06-26 | 2009-12-16 | コールド スプリング ハーバー ラボラトリー | Methods and compositions for measuring methylation profiles |
AU2003279101A1 (en) | 2002-10-02 | 2004-04-23 | Northwestern University | Methylation profile of cancer |
EP1606417A2 (en) | 2003-03-07 | 2005-12-21 | Rubicon Genomics Inc. | In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna |
EP2339024B1 (en) | 2003-10-21 | 2014-12-31 | Orion Genomics, LLC | Methods for quantitative determination of methylation density in a DNA locus |
WO2005085477A1 (en) | 2004-03-02 | 2005-09-15 | Orion Genomics Llc | Differential enzymatic fragmentation by whole genome amplification |
-
2004
- 2004-10-21 EP EP10011640.9A patent/EP2339024B1/en not_active Not-in-force
- 2004-10-21 WO PCT/US2004/035177 patent/WO2005040399A2/en active Application Filing
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- 2004-10-21 EP EP04796072A patent/EP1682677B1/en active Active
- 2004-10-21 JP JP2006536855A patent/JP4887154B2/en not_active Expired - Fee Related
- 2004-10-21 AT AT04796072T patent/ATE553218T1/en active
- 2004-10-21 AU AU2004284456A patent/AU2004284456B2/en not_active Ceased
- 2004-10-21 CA CA2542542A patent/CA2542542C/en not_active Expired - Fee Related
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- 2004-10-21 EP EP04796212A patent/EP1689887B1/en active Active
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- 2004-10-21 JP JP2006536815A patent/JP4917891B2/en not_active Expired - Fee Related
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5405760A (en) * | 1992-04-30 | 1995-04-11 | New England Biolabs, Inc. | Process for producing recombinant McrBC endonuclease and cleavage of methylated DNA |
Non-Patent Citations (2)
Title |
---|
CHU ET AL: "The use of real-time quantitative polymerase chain reaction to detect hypermethylation of the CpG islands in the promoter region flanking the GSTP1 gene to diagnose prostate carcinoma", THE JOURNAL OF UROLOGY, vol. 167, April 2002 (2002-04-01), pages 1865, XP005543746 * |
FRAGA ET AL: "DNA methylation: A profile of methods and applications", BIOTECHNIQUES, vol. 33, no. 3, 2002, pages 632 - 649, XP001107174 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9394565B2 (en) | 2003-09-05 | 2016-07-19 | Agena Bioscience, Inc. | Allele-specific sequence variation analysis |
US9249456B2 (en) | 2004-03-26 | 2016-02-02 | Agena Bioscience, Inc. | Base specific cleavage of methylation-specific amplification products in combination with mass analysis |
US8962247B2 (en) | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
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