WO2005042784A3 - Device and method for high-throughput quantification of mrna from whole blood - Google Patents

Device and method for high-throughput quantification of mrna from whole blood Download PDF

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Publication number
WO2005042784A3
WO2005042784A3 PCT/US2004/036309 US2004036309W WO2005042784A3 WO 2005042784 A3 WO2005042784 A3 WO 2005042784A3 US 2004036309 W US2004036309 W US 2004036309W WO 2005042784 A3 WO2005042784 A3 WO 2005042784A3
Authority
WO
WIPO (PCT)
Prior art keywords
mrna
whole blood
throughput quantification
kit
automated system
Prior art date
Application number
PCT/US2004/036309
Other languages
French (fr)
Other versions
WO2005042784A2 (en
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Res Ct Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Res Ct Inc filed Critical Hitachi Chemical Co Ltd
Priority to JP2006538406A priority Critical patent/JP4594321B2/en
Priority to AT04796890T priority patent/ATE491042T1/en
Priority to EP04796890A priority patent/EP1682678B1/en
Priority to DE602004030472T priority patent/DE602004030472D1/en
Publication of WO2005042784A2 publication Critical patent/WO2005042784A2/en
Publication of WO2005042784A3 publication Critical patent/WO2005042784A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Abstract

Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
PCT/US2004/036309 2003-10-30 2004-10-29 Device and method for high-throughput quantification of mrna from whole blood WO2005042784A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006538406A JP4594321B2 (en) 2003-10-30 2004-10-29 Apparatus and method for quantifying mRNA from whole blood with high efficiency
AT04796890T ATE491042T1 (en) 2003-10-30 2004-10-29 APPARATUS AND METHOD FOR QUANTIFYING MRNA FROM WHOLE BLOOD WITH HIGH THROUGHPUT
EP04796890A EP1682678B1 (en) 2003-10-30 2004-10-29 Device and method for high-throughput quantification of mrna from whole blood
DE602004030472T DE602004030472D1 (en) 2003-10-30 2004-10-29 DEVICE AND METHOD FOR QUANTIFYING MRNA FROM FULL BLOOD WITH HIGH THROUGHPUT

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US69896703A 2003-10-30 2003-10-30
US10/698,967 2003-10-30
US10/796,298 2004-03-09
US10/796,298 US7745180B2 (en) 2002-04-24 2004-03-09 Device and method for high-throughput quantification of mRNA from whole blood

Publications (2)

Publication Number Publication Date
WO2005042784A2 WO2005042784A2 (en) 2005-05-12
WO2005042784A3 true WO2005042784A3 (en) 2005-10-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/036309 WO2005042784A2 (en) 2003-10-30 2004-10-29 Device and method for high-throughput quantification of mrna from whole blood

Country Status (7)

Country Link
US (6) US7745180B2 (en)
EP (4) EP1953245B1 (en)
JP (3) JP4594321B2 (en)
CN (4) CN101696409B (en)
AT (4) ATE466110T1 (en)
DE (3) DE602004026947D1 (en)
WO (1) WO2005042784A2 (en)

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US9416416B2 (en) 2007-10-01 2016-08-16 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection/transport compositions and methods
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US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
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EP1997912A3 (en) 2008-12-10
ATE442458T1 (en) 2009-09-15
US8101344B2 (en) 2012-01-24
JP2007509633A (en) 2007-04-19
US20070224632A1 (en) 2007-09-27
US20080009009A1 (en) 2008-01-10
CN101696442B (en) 2013-04-17
JP4594321B2 (en) 2010-12-08
EP2058405A1 (en) 2009-05-13
US8076105B2 (en) 2011-12-13
EP1682678B1 (en) 2010-12-08
US7745180B2 (en) 2010-06-29
EP1953245A1 (en) 2008-08-06
DE602004023143D1 (en) 2009-10-22
EP1953245B1 (en) 2009-09-09
ATE517193T1 (en) 2011-08-15
CN101696409B (en) 2012-06-27
JP2010162050A (en) 2010-07-29
DE602004026947D1 (en) 2010-06-10
EP2058405B1 (en) 2011-07-20
EP1682678A2 (en) 2006-07-26
CN101696448A (en) 2010-04-21
US7968288B1 (en) 2011-06-28
JP5160592B2 (en) 2013-03-13
JP2010220619A (en) 2010-10-07
US20040265864A1 (en) 2004-12-30
WO2005042784A2 (en) 2005-05-12
US20060199212A1 (en) 2006-09-07
ATE491042T1 (en) 2010-12-15
DE602004030472D1 (en) 2011-01-20
EP1997912A2 (en) 2008-12-03
CN1997752A (en) 2007-07-11
JP5160591B2 (en) 2013-03-13
EP1997912B1 (en) 2010-04-28
US7981608B2 (en) 2011-07-19
US7939300B2 (en) 2011-05-10
US20070224631A1 (en) 2007-09-27
CN101696409A (en) 2010-04-21
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