WO2005047523A3 - Short cycle methods for sequencing polynucleotides - Google Patents

Short cycle methods for sequencing polynucleotides Download PDF

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Publication number
WO2005047523A3
WO2005047523A3 PCT/US2004/037613 US2004037613W WO2005047523A3 WO 2005047523 A3 WO2005047523 A3 WO 2005047523A3 US 2004037613 W US2004037613 W US 2004037613W WO 2005047523 A3 WO2005047523 A3 WO 2005047523A3
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WO
WIPO (PCT)
Prior art keywords
short cycle
sequencing polynucleotides
cycle methods
sequencing
methods
Prior art date
Application number
PCT/US2004/037613
Other languages
French (fr)
Other versions
WO2005047523A2 (en
Inventor
Stanley N Lapidus
Philip Richard Buzby
Timothy A Harris
Original Assignee
Helicos Biosciences Corp
Stanley N Lapidus
Philip Richard Buzby
Timothy A Harris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34595948&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2005047523(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Helicos Biosciences Corp, Stanley N Lapidus, Philip Richard Buzby, Timothy A Harris filed Critical Helicos Biosciences Corp
Priority to CA002545619A priority Critical patent/CA2545619A1/en
Priority to EP04810723A priority patent/EP1692312A4/en
Publication of WO2005047523A2 publication Critical patent/WO2005047523A2/en
Publication of WO2005047523A3 publication Critical patent/WO2005047523A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near of full completion.
PCT/US2004/037613 2003-11-12 2004-11-12 Short cycle methods for sequencing polynucleotides WO2005047523A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002545619A CA2545619A1 (en) 2003-11-12 2004-11-12 Short cycle methods for sequencing polynucleotides
EP04810723A EP1692312A4 (en) 2003-11-12 2004-11-12 Short cycle methods for sequencing polynucleotides

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US51986203P 2003-11-12 2003-11-12
US60/519,862 2003-11-12
US54627704P 2004-02-19 2004-02-19
US60/546,277 2004-02-19
US54761104P 2004-02-24 2004-02-24
US60/547,611 2004-02-24

Publications (2)

Publication Number Publication Date
WO2005047523A2 WO2005047523A2 (en) 2005-05-26
WO2005047523A3 true WO2005047523A3 (en) 2005-12-22

Family

ID=34595948

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/037613 WO2005047523A2 (en) 2003-11-12 2004-11-12 Short cycle methods for sequencing polynucleotides

Country Status (4)

Country Link
US (7) US7169560B2 (en)
EP (1) EP1692312A4 (en)
CA (1) CA2545619A1 (en)
WO (1) WO2005047523A2 (en)

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