WO2005087920A3 - Recombinant chondroitinase abc i and uses thereof - Google Patents

Recombinant chondroitinase abc i and uses thereof Download PDF

Info

Publication number
WO2005087920A3
WO2005087920A3 PCT/US2005/008194 US2005008194W WO2005087920A3 WO 2005087920 A3 WO2005087920 A3 WO 2005087920A3 US 2005008194 W US2005008194 W US 2005008194W WO 2005087920 A3 WO2005087920 A3 WO 2005087920A3
Authority
WO
WIPO (PCT)
Prior art keywords
chondroitinase abc
treating
gags
enzymes
relates
Prior art date
Application number
PCT/US2005/008194
Other languages
French (fr)
Other versions
WO2005087920A2 (en
Inventor
Vikas Prabhakar
Ishan Capila
Rahul Raman
Carlos Bosques
Kevin Pojasek
Ram Sasisekharan
Original Assignee
Massachusetts Inst Technology
Vikas Prabhakar
Ishan Capila
Rahul Raman
Carlos Bosques
Kevin Pojasek
Ram Sasisekharan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology, Vikas Prabhakar, Ishan Capila, Rahul Raman, Carlos Bosques, Kevin Pojasek, Ram Sasisekharan filed Critical Massachusetts Inst Technology
Priority to CA002558984A priority Critical patent/CA2558984A1/en
Priority to EP05735137A priority patent/EP1737954A2/en
Publication of WO2005087920A2 publication Critical patent/WO2005087920A2/en
Publication of WO2005087920A3 publication Critical patent/WO2005087920A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention relates to chondroitinase ABC I and uses thereof. In particular, the invention relates to recombinant and modified chondroitinase ABC I, their production and their uses. The chondroitinase ABC I enzymes of the invention are useful for a variety of purposes, including degrading and analyzing polysaccharides such as glycosaminoglycans (GAGs). These GAGs can include chondroitin sulfate, dermatan sulfate, unsulfated chondroitin and hyaluronan. The chondroitinase ABC I enzymes can also be used in therapeutic methods such as promoting nerve regeneration, promoting stroke recovery, treating spinal cord injury, treating epithelial disease, treating infections and treating cancer.
PCT/US2005/008194 2004-03-10 2005-03-10 Recombinant chondroitinase abc i and uses thereof WO2005087920A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002558984A CA2558984A1 (en) 2004-03-10 2005-03-10 Recombinant chondroitinase abc i and uses thereof
EP05735137A EP1737954A2 (en) 2004-03-10 2005-03-10 Recombinant chondroitinase abc i and uses thereof

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US55223204P 2004-03-10 2004-03-10
US60/552,232 2004-03-10
US57891704P 2004-06-10 2004-06-10
US60/578,917 2004-06-10
US62505204P 2004-11-03 2004-11-03
US60/625,052 2004-11-03

Publications (2)

Publication Number Publication Date
WO2005087920A2 WO2005087920A2 (en) 2005-09-22
WO2005087920A3 true WO2005087920A3 (en) 2006-02-02

Family

ID=34965511

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/008194 WO2005087920A2 (en) 2004-03-10 2005-03-10 Recombinant chondroitinase abc i and uses thereof

Country Status (4)

Country Link
US (5) US7507570B2 (en)
EP (1) EP1737954A2 (en)
CA (1) CA2558984A1 (en)
WO (1) WO2005087920A2 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003527822A (en) * 1998-08-27 2003-09-24 マサチューセッツ インスティテュート オブ テクノロジー Rationally designed heparinases from heparinases I and II
CA2370539C (en) * 1999-04-23 2009-01-06 Massachusetts Institute Of Technology System and method for notating polymers
AU4351201A (en) * 2000-03-08 2001-09-17 Massachusetts Inst Technology Heparinase iii and uses thereof
ATE426805T1 (en) * 2000-09-12 2009-04-15 Massachusetts Inst Technology METHODS AND PRODUCTS ASSOCIATED WITH LOW MOLECULAR HEPARIN
WO2002032406A2 (en) 2000-10-18 2002-04-25 Massachusetts Institute Of Technology Methods and products related to pulmonary delivery of polysaccharides
EP1575534B1 (en) * 2002-05-03 2013-04-10 Massachusetts Institute Of Technology D4,5 glycuronidase and uses thereof
US8183350B2 (en) 2002-05-04 2012-05-22 Acorda Therapeutics, Inc. Compositions and methods for promoting neuronal outgrowth
WO2004055491A2 (en) 2002-05-20 2004-07-01 Massachusetts Institute Of Technology Novel method for sequence determination using nmr
WO2004017044A2 (en) * 2002-08-15 2004-02-26 Acorda Therapeutics, Inc. Chimeric protein
JP4606712B2 (en) * 2003-01-08 2011-01-05 マサチューセッツ インスティテュート オブ テクノロジー 2-O sulfatase compositions and related methods
US7959914B2 (en) * 2003-05-16 2011-06-14 Acorda Therapeutics, Inc. Methods of reducing extravasation of inflammatory cells
EP1631237B1 (en) * 2003-05-16 2016-11-30 Acorda Therapeutics, Inc. Proteoglycan degrading mutants for treatment of cns
US20080025963A1 (en) * 2003-05-16 2008-01-31 Gruskin Elliott A Compositions and methods for the treatment of CNS injuries
US7507570B2 (en) 2004-03-10 2009-03-24 Massachusetts Institute Of Technology Recombinant chondroitinase ABC I and uses thereof
WO2005111627A2 (en) * 2004-04-15 2005-11-24 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
US20060127950A1 (en) * 2004-04-15 2006-06-15 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
CA2566731C (en) * 2004-05-18 2012-07-24 Acorda Therapeutics, Inc. Methods of purifying chondroitinase and stable formulations thereof
US8765122B2 (en) * 2004-06-14 2014-07-01 The Research Foundation Of State University Of New York Nanosphere/microsphere delivery system for the treatment of spinal cord injury
CA2614068A1 (en) 2004-06-29 2006-08-24 Massachusetts Institute Of Technology Methods and compositions related to the modulation of intercellular junctions
US20060154894A1 (en) * 2004-09-15 2006-07-13 Massachusetts Institute Of Technology Biologically active surfaces and methods of their use
CA2623635C (en) 2005-09-26 2013-04-02 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase abci mutants
EP2008096A2 (en) * 2006-04-03 2008-12-31 Massachusetts Institute of Technology Glycomic patterns for the detection of disease
KR101368742B1 (en) * 2006-05-19 2014-02-28 세이가가쿠 고교 가부시키가이샤 Ameliorating agent for brain damage
WO2008045970A2 (en) 2006-10-10 2008-04-17 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase abci mutants
AU2014200000B2 (en) * 2006-10-10 2016-04-21 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase abci mutants
AU2016206267B2 (en) * 2006-10-10 2018-07-05 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase abci mutants
WO2008085912A1 (en) 2007-01-05 2008-07-17 Massachusetts Institute Of Technology Compositions of and methods of using sulfatases from flavobacterium heparinum
GB0712302D0 (en) * 2007-06-22 2007-08-01 Cambridge Entpr Ltd Chrondroitinase polypeptides
US8450106B2 (en) * 2007-10-17 2013-05-28 The Ohio State University Research Foundation Oncolytic virus
US20090286289A1 (en) * 2008-03-10 2009-11-19 Pang Danny Z Production of Hyaluronate Unsaturated Disaccharides and its Application
US20140212404A1 (en) * 2012-11-14 2014-07-31 Khizer Khaderi Compositions and Methods for Treating Injuries to the Visual System of a Human
WO2014201220A1 (en) * 2013-06-12 2014-12-18 The Board Of Trustees Of The University Of Illinois Methods for detecting prostate cancer
ES2771448T3 (en) 2013-06-18 2020-07-06 Univ Helsinki Protamine in the treatment of neuronal injuries
WO2015048523A2 (en) * 2013-09-27 2015-04-02 Ohio State Innovation Foundation Mutant chondroitinase abc i compositions and methods of their use
KR102167443B1 (en) * 2019-03-25 2020-10-19 주식회사 카티프라임 In vitro osteoarthritis condition model and drug screening method for osteoarthritis using the same
CN111593040B (en) * 2020-06-20 2022-08-30 山东大学 Dermatan sulfate lyase and application thereof
CN116042593B (en) * 2023-01-16 2023-07-14 山东省农业科学院畜牧兽医研究所 Clostridium perfringens bacteriophage lyase and application thereof in preparation of clostridium perfringens infection resistant medicines

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0613949A2 (en) * 1993-02-24 1994-09-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
WO2003102160A2 (en) * 2002-06-03 2003-12-11 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US739904A (en) * 1902-08-05 1903-09-29 Orville Mcnorton Jr Music holder and turner.
ZA912770B (en) 1990-04-16 1992-01-29 Bethesda Eye Inst Enzymatic disinsertion of vitreous body
JPH04270327A (en) 1991-02-26 1992-09-25 Fuji Photo Optical Co Ltd Exposure correcting device for camera
US5773277A (en) 1992-06-26 1998-06-30 Seikagaku Kogyo Kabushiki Kaisha Crystalline chondroitinase isolated from Proteus vulgaris ATCC 6896
US6001630A (en) 1992-06-26 1999-12-14 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Pharmaceutical compositions of chondroitinase ABC isolated from Proteus vulgaris ATCC 6896
US5496718A (en) 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
EP0702715A4 (en) 1993-04-23 2000-01-19 American Cyanamid Co CLONING AND EXPRESSION OF THE CHONDROITINASE I AND II GENES FROM $i(P. VULGARIS)
US5578480A (en) 1993-04-23 1996-11-26 American Cyanamid Company Methods for the isolation and purification of the recombinantly expressed chondroitinase I and II enzymes from P. vulgaris
US5498536A (en) * 1994-04-22 1996-03-12 American Cyanamid Company Chondroitinase II from Proteus vulgaris
EP0756636A4 (en) 1994-04-22 1997-12-17 American Cyanamid Co Chondroitinases i and ii, methods of preparation, and use thereof
US5525500A (en) 1994-04-22 1996-06-11 American Cyanamid Company Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
US6093563A (en) 1994-07-08 2000-07-25 Ibex Technologies R And D, Inc. Chondroitin lyase enzymes
US5997863A (en) 1994-07-08 1999-12-07 Ibex Technologies R And D, Inc. Attenuation of wound healing processes
US5888798A (en) 1995-06-07 1999-03-30 American Cyanamid Company Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
WO1997016556A1 (en) 1995-10-30 1997-05-09 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from heparinase i
EP0875253B1 (en) 1997-05-02 2005-04-06 Seikagaku Corporation Chondroitinase compositions
JP2000044601A (en) 1998-07-29 2000-02-15 Seikagaku Kogyo Co Ltd New galactosaminoglucan
JP2003527822A (en) 1998-08-27 2003-09-24 マサチューセッツ インスティテュート オブ テクノロジー Rationally designed heparinases from heparinases I and II
US7056504B1 (en) 1998-08-27 2006-06-06 Massachusetts Institute Of Technology Rationally designed heparinases derived from heparinase I and II
CA2370539C (en) 1999-04-23 2009-01-06 Massachusetts Institute Of Technology System and method for notating polymers
ATE413886T1 (en) 1999-11-17 2008-11-15 Biomarin Pharm Inc REDUCING ANGIOGENESIS USING CHONDROITIN SULPHATE DEGRADING ENZYMES
AU4351201A (en) 2000-03-08 2001-09-17 Massachusetts Inst Technology Heparinase iii and uses thereof
US7094165B2 (en) * 2000-03-17 2006-08-22 Delbert Clarke Pile Apparatus and method for a retractable basketball backboard and hoop assembly
ATE426805T1 (en) 2000-09-12 2009-04-15 Massachusetts Inst Technology METHODS AND PRODUCTS ASSOCIATED WITH LOW MOLECULAR HEPARIN
WO2002032406A2 (en) 2000-10-18 2002-04-25 Massachusetts Institute Of Technology Methods and products related to pulmonary delivery of polysaccharides
CA2441986A1 (en) 2001-03-27 2002-10-03 Massachusetts Institute Of Technology Methods and products related to fgf dimerization
GB0205022D0 (en) 2002-03-04 2002-04-17 Univ Cambridge Tech Materials and methods for the treatment of cns damage
EP1575534B1 (en) 2002-05-03 2013-04-10 Massachusetts Institute Of Technology D4,5 glycuronidase and uses thereof
WO2004055491A2 (en) 2002-05-20 2004-07-01 Massachusetts Institute Of Technology Novel method for sequence determination using nmr
JP4606712B2 (en) 2003-01-08 2011-01-05 マサチューセッツ インスティテュート オブ テクノロジー 2-O sulfatase compositions and related methods
EP1631237B1 (en) 2003-05-16 2016-11-30 Acorda Therapeutics, Inc. Proteoglycan degrading mutants for treatment of cns
US7507570B2 (en) 2004-03-10 2009-03-24 Massachusetts Institute Of Technology Recombinant chondroitinase ABC I and uses thereof
WO2005111627A2 (en) 2004-04-15 2005-11-24 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
WO2005110438A2 (en) 2004-04-15 2005-11-24 Massachusetts Institute Of Technology Methods and products related to the intracellular delivery of polysaccharides
US20060127950A1 (en) 2004-04-15 2006-06-15 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
CA2614068A1 (en) 2004-06-29 2006-08-24 Massachusetts Institute Of Technology Methods and compositions related to the modulation of intercellular junctions
US20060154894A1 (en) 2004-09-15 2006-07-13 Massachusetts Institute Of Technology Biologically active surfaces and methods of their use
WO2006076627A2 (en) 2005-01-12 2006-07-20 Massachusetts Institute Of Technology Methods and compositions related to modulating the extracellular stem cell environment
US20090105463A1 (en) 2005-03-29 2009-04-23 Massachusetts Institute Of Technology Compositions of and Methods of Using Oversulfated Glycosaminoglycans
US20090156477A1 (en) 2005-03-29 2009-06-18 Massachusetts Institute Of Technology Compositions and Methods for Regulating Inflammatory Responses
US7429373B2 (en) * 2005-06-24 2008-09-30 Air Products And Chemicals, Inc. Process for autothermal generation of hydrogen
EP2008096A2 (en) 2006-04-03 2008-12-31 Massachusetts Institute of Technology Glycomic patterns for the detection of disease
WO2008085912A1 (en) 2007-01-05 2008-07-17 Massachusetts Institute Of Technology Compositions of and methods of using sulfatases from flavobacterium heparinum

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0613949A2 (en) * 1993-02-24 1994-09-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
WO2003102160A2 (en) * 2002-06-03 2003-12-11 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRADBURY E J ET AL: "Chondroitinase ABC promotes regeneration and functional recovery following spinal cord injury", SOCIETY FOR NEUROSCIENCE ABSTRACTS, SOCIETY FOR NEUROSCIENCE, US, vol. 27, no. part 2, 2001, pages 1835, XP002244997, ISSN: 0190-5295 *
DENHOLM E M ET AL: "ANTI-TUMOR ACTIVITIES OF CHONDROITINASE AC AND CHONDROITINASE B: INHIBITION OF ANGIOGENESIS, PROLIFERATION AND INVASION", EUROPEAN JOURNAL OF PHARMACOLOGY, AMSTERDAM, NL, vol. 416, no. 3, 2001, pages 213 - 221, XP001002664, ISSN: 0014-2999 *

Also Published As

Publication number Publication date
US8338119B2 (en) 2012-12-25
US20070148157A1 (en) 2007-06-28
US7592152B2 (en) 2009-09-22
CA2558984A1 (en) 2005-09-22
US20070202563A1 (en) 2007-08-30
WO2005087920A2 (en) 2005-09-22
US20070148740A1 (en) 2007-06-28
US20070224670A1 (en) 2007-09-27
EP1737954A2 (en) 2007-01-03
US7507570B2 (en) 2009-03-24
US7662604B2 (en) 2010-02-16
US20060078959A1 (en) 2006-04-13
US7553950B2 (en) 2009-06-30

Similar Documents

Publication Publication Date Title
WO2005087920A3 (en) Recombinant chondroitinase abc i and uses thereof
Fallacara et al. Hyaluronic acid in the third millennium
WO2007070547A3 (en) Treatment of arthritis and other musculoskeletal disorders with crosslinked hyaluronic acid
WO2007084769A3 (en) Microalgae-derived compositions for improving the health and appearance of skin
WO2003102160A3 (en) Rationally designed polysaccharide lyases derived from chondroitinase b
WO2006071802A3 (en) Treatment of stroke and other acute neural degenerative disorders using postpartum derived cells
US20080274999A1 (en) Hyaluronic Acid Fraction with Moisturizing and Anti-Wrinkle Properties
WO2006032500A3 (en) Immunogenic composition
WO2011056688A3 (en) Molecular profiling for personalized medicine
WO2006105313A3 (en) Compositions of and methods of using oversulfated glycosaminoglycans
WO2008005303A3 (en) Thiazolopyrimidine modulators of trpv1
WO2007140231A3 (en) Low molecular weight heparin composition and uses thereof
WO2004062592A3 (en) 2-o sulfatase compositions and related methods
HK1118011A1 (en) Antitumoral bioconjugates of hyaluronic acid or its derivatives obtained by indirect chemical conjugation, and their use in the pharmaceutical field
ATE426805T1 (en) METHODS AND PRODUCTS ASSOCIATED WITH LOW MOLECULAR HEPARIN
EP2772260A3 (en) Therapeutic compositions comprising hyaluronan and therapeutic antibodies as well as methods of treatment
US8071757B2 (en) Aryl/alkyl vinyl sulfone hyaluronic acid derivatives
ATE488548T1 (en) METHOD FOR PRODUCING GLUCOMANN ANGLE PARTICLES
RU2017124620A (en) Vaccination of Cyclodextrin with Amide Bonds to Hyaluronic Acid Cross-Linked with Ether and Their Use
WO2007025212A3 (en) Use of sodium channel blockers for the treatment of visceral pain or pain caused by cancer treatment
WO2009102880A3 (en) Structure and bioactivity of the polysaccharides and oligomers in medicinal plant dendrobium huoshanense
IL172477A0 (en) Viscosupplementation with algal polysaccharides in the treatment of arthritis
WO2007149554A3 (en) Methods for restoring neural function
SI2794667T1 (en) Low anticoagulant heparins
WO2007149594A3 (en) Modified ribonucleases

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2558984

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 2005735137

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2005735137

Country of ref document: EP