WO2005117756A3 - Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions - Google Patents

Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions Download PDF

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Publication number
WO2005117756A3
WO2005117756A3 PCT/US2005/018214 US2005018214W WO2005117756A3 WO 2005117756 A3 WO2005117756 A3 WO 2005117756A3 US 2005018214 W US2005018214 W US 2005018214W WO 2005117756 A3 WO2005117756 A3 WO 2005117756A3
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WO
WIPO (PCT)
Prior art keywords
acid
granular starch
enzyme compositions
stable alpha
starch hydrolyzing
Prior art date
Application number
PCT/US2005/018214
Other languages
French (fr)
Other versions
WO2005117756A2 (en
Inventor
Nigel Dunn-Coleman
Susan M Fiske
Suzanne E Lantz
Paulien Neefe-Kruithof
Michael J Pepsin
Jayarama K Shetty
Original Assignee
Genencor Int
Nigel Dunn-Coleman
Susan M Fiske
Suzanne E Lantz
Paulien Neefe-Kruithof
Michael J Pepsin
Jayarama K Shetty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2004/040040 external-priority patent/WO2005118800A1/en
Application filed by Genencor Int, Nigel Dunn-Coleman, Susan M Fiske, Suzanne E Lantz, Paulien Neefe-Kruithof, Michael J Pepsin, Jayarama K Shetty filed Critical Genencor Int
Priority to KR1020137026016A priority Critical patent/KR101545424B1/en
Priority to AU2005249422A priority patent/AU2005249422B2/en
Priority to CA2567485A priority patent/CA2567485C/en
Priority to MXPA06013600A priority patent/MXPA06013600A/en
Priority to EP05763790A priority patent/EP1749092A2/en
Priority to CN200580040938.5A priority patent/CN101068922B/en
Priority to ES05816997T priority patent/ES2370768T3/en
Priority to PL05816997T priority patent/PL1824970T3/en
Priority to CA2589725A priority patent/CA2589725C/en
Priority to EP05816997A priority patent/EP1824970B1/en
Priority to AT05816997T priority patent/ATE519842T1/en
Priority to DK05816997.0T priority patent/DK1824970T3/en
Publication of WO2005117756A2 publication Critical patent/WO2005117756A2/en
Publication of WO2005117756A3 publication Critical patent/WO2005117756A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.
PCT/US2005/018214 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions WO2005117756A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
KR1020137026016A KR101545424B1 (en) 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
AU2005249422A AU2005249422B2 (en) 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
CA2567485A CA2567485C (en) 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
MXPA06013600A MXPA06013600A (en) 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions.
EP05763790A EP1749092A2 (en) 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
DK05816997.0T DK1824970T3 (en) 2004-11-30 2005-10-07 Thricoderma reesei glycoamylase and its homologs
ES05816997T ES2370768T3 (en) 2004-11-30 2005-10-07 GLUCOAMYLASE OF TRICHODERMA REESEI AND HOMOLOGISTS OF THE SAME.
CN200580040938.5A CN101068922B (en) 2004-11-30 2005-10-07 Trichoderma reesei glucoamylase and homologs thereof
PL05816997T PL1824970T3 (en) 2004-11-30 2005-10-07 Trichoderma reesei glucoamylase and homologs thereof
CA2589725A CA2589725C (en) 2004-11-30 2005-10-07 Trichoderma reesei glucoamylase and homologs thereof
EP05816997A EP1824970B1 (en) 2004-11-30 2005-10-07 Trichoderma reesei glucoamylase and homologs thereof
AT05816997T ATE519842T1 (en) 2004-11-30 2005-10-07 TRICHODERMA REESEI GLUCOAMYLASE AND HOMOLOGUE THEREOF

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US57517504P 2004-05-27 2004-05-27
US60/575,175 2004-05-27
US60543704P 2004-08-30 2004-08-30
US60/605,437 2004-08-30
USPCT/US2004/040040 2004-11-30
PCT/US2004/040040 WO2005118800A1 (en) 2004-05-27 2004-11-30 Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
USPCT/US2004/041276 2004-12-09
PCT/US2004/041276 WO2005117953A1 (en) 2004-05-27 2004-12-09 Heterologous expression of an aspergillus kawachi acid-stable alpha amylase
US64792505P 2005-01-28 2005-01-28
US60/647,925 2005-01-28

Publications (2)

Publication Number Publication Date
WO2005117756A2 WO2005117756A2 (en) 2005-12-15
WO2005117756A3 true WO2005117756A3 (en) 2006-03-02

Family

ID=37575011

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2005/018212 WO2005118795A2 (en) 2004-05-27 2005-05-24 Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
PCT/US2005/018214 WO2005117756A2 (en) 2004-05-27 2005-05-24 Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2005/018212 WO2005118795A2 (en) 2004-05-27 2005-05-24 Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis

Country Status (8)

Country Link
US (6) US20080199927A1 (en)
EP (2) EP1749092A2 (en)
KR (1) KR101545424B1 (en)
CN (1) CN1997735B (en)
AU (1) AU2005249422B2 (en)
CA (2) CA2567485C (en)
MX (1) MXPA06013600A (en)
WO (2) WO2005118795A2 (en)

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