WO2006012413A1 - Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same - Google Patents
Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same Download PDFInfo
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- WO2006012413A1 WO2006012413A1 PCT/US2005/025875 US2005025875W WO2006012413A1 WO 2006012413 A1 WO2006012413 A1 WO 2006012413A1 US 2005025875 W US2005025875 W US 2005025875W WO 2006012413 A1 WO2006012413 A1 WO 2006012413A1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
- C07K16/1289—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Mycobacteriaceae (F)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
Definitions
- the present invention relates to diagnostic tests for detecting microbial-based diseases and conditions, and more particularly for diagnostic tests and methods for detecting tuberculosis.
- the sputum test for pulmonary TB is not always effective, particularly if there are no detectable bacteria in the sputum, or no sputum sample can be obtained.
- this diagnostic test requires microscopy and/or culture of the bacteria to confirm the diagnosis, neither of which is especially suitable to diagnosis in the field.
- Using cerebro/spinal fluid for diagnosis of TB- meningitis is also problematic, particularly in the field since, once again, microscopy and/or culture of the bacteria and/or an ELISA test is usually required to confirm the diagnosis.
- Blood tests for TB are also known, but have a poor track record, being complex and unreliable. Urine tests are simpler and more reliable, but current methods require processing of the urine before performing the diagnostic test - such processing usually involving concentration of the urine. Among the newly developed methods antibody tests against a number of mycobacterial antigens have been developed, but none of these tests has so far reached the needed specificity for routine diagnostic purpose. The drop of sensitivity in HTV positive cases is also a major constraint. A different approach is to measure immune responses to Mycobacterium tuberculosis specific antigens like ESAT-6, but so far the differentiation between latent TB infection and TB disease is not possible.
- Tuberculosis is an extremely complex pathology existing in multiple forms, but always starting as an airborne infection. Pulmonary tuberculosis occurs immediately at the entry point of the microorganism and extrapulmonary tuberculosis is the result of further penetration into body of the patient with the most widespread examples of tuberculous meningitis and bone tuberculosis. Complexity of the pathology determines multitude of various approaches tried during this century of modern medicine. Furthermore clinical and radiographic manifestations of HIV-related pulmonary tuberculosis are dramatically altered by immunodeficiency. These factors severely limit our capability of early symptomatic recognition of tuberculosis in HTVTTB patients and also increase the danger of TB transmission to relatives and caregivers of such patients.
- Mycobacteria can potentially be recovered from a variety of clinical specimens, including upper respiratory collections (sputum, bronchial washes, bronchoalveolar lavage, bronchial biopsies and such); urine, feces, blood, cerebrospinal fluid (CSF), tissue biopsies, and deep needle aspirations of virtually any tissue or organ.
- Bacterial culture remains the gold standard in the diagnosis of tuberculosis, but it can take up to 6-8 weeks to make a conclusive diagnosis.
- Direct microscopy of sputum smears More than a century ago, Robert Koch identified the ethiologic agent of tuberculosis by staining it and culturing it from clinical specimens. Today, the diagnosis of tuberculosis is usually established using staining and culturing techniques that do not differ substantially from those that Koch used. Direct microscopy of sputum is the norm for the diagnosis of tuberculosis in developing countries today and it is the benchmark against which the efficiency of any new test must be assessed. It is applied to pulmonary tuberculosis, but is not very useful for children or for patients with initial stages of pulmonary tuberculosis. PCR-based assays.
- the Tuberculosis Skin Test This is the probably oldest immunological test for tuberculosis. A small amount of substance called PPD Tuberculin is placed just under the top layer of the skin on the forearm with a small needle. The test is read 48 to 72 hours after it has been given. Generally, a swelling of 10 mm. or more is considered positive. Many developing countries use BCG vaccination to protect against TB. After BCG vaccination, the PPD skin test usually becomes positive. Results of the skin test vary dependent on the quality of the PPD antigen, reactivity of the immune system and probably even race of the individual. This test also does not provide an unequivocal indication about the stage and location of the infection.
- Serological tests for M. tuberculosis This approach, based on the detection of antibody immune response to mycobacterial antigens is one of the most widely used in research and clinical environments.
- AU serological tests have approximately the same sensitivity and specificity if they use purified antigens.
- the sensitivity of the best tests is in a range of 80% for smear-positive cases and 60-70% for smear negative cases.
- the reported specificity is generally high and is in a range of 95-100%.
- Skin test has sufficient sensitivity, but takes a long time and does not provide information about stage of pathological process and do not sufficiently differentiate infected and vaccinated individuals.
- PCR tests are widely used in developed countries, but are complex, expensive and are not sensitive enough to justify their use as a screening test in developing countries.
- a preferred method for rapid diagnosis of infectious diseases is based on the detection of a bacterial antigen in the patient sample, what provides unequivocal proof of active infectious process caused by specific pathogen. The concept of using a direct antigen test for detection of mycobacterial infections was described in several publications.
- Bacterial polysaccharides are composed of monosaccharides uncommon to humans and therefore resistant to cleavage by human enzymes. This enables their secretion in urine in irnmunochemically intact forms suitable for detection by a polysaccharide-specific immunoassay. Extremely low concentrations of bacterial polysaccharides secreted in urine require very high sensitivity of the immunoassay in order to use it as a screening procedure.
- an antigenically active isoform of lipoarabinomannan from mycobacterium tuberculosis prepared by oxidation of LAM using mild oxidation methods such as treatment with low concentrations of NaIO 4 .
- the antigenically active isoform of LAM generated by mild oxidation methods, is used to prepare highly specific, highly pure antibodies to inactivated mycobacterium, more particularly to surface polysaccharides such as LAM, for use in the detection of polysaccharides (e.g. LAM) in urine, sputum, blood, tissue or other samples from patients of interest.
- Other embodiments use the highly specific, highly pure antibody raised to the antigenically active form of LAM to diagnose tuberculosis in patients of interest.
- an enriched antibody population highly specific for an antigen of a surface polysaccharide from a mycobacterium may be enriched by having been raised in an environment that maintains antigenically active antigen.
- the antibody is enriched by exclusion of antibodies that recognize relatively inactive antigen.
- the mycobacterium may be Mycobacterium tuberculosis.
- the surface polysaccharide may be lipoarabinomannan (LAM).
- a process for producing an enriched antibody highly specific to an antigen of a mycobacterium comprises raising and isolating antibody to antigen from mycobacteria; and separating from the isolated antibodies that population of antibodies which is specific to relatively inactive antigen to produce isolated enriched antibody.
- process for producing an enriched antibody highly specific to an antigen of a mycobacterium comprises isolating antigen from mycobacteria under conditions maintaining antigenic activity; and raising antibodies to the isolated antigen while maintaining its antigenic activity.
- a process for producing an enriched antibody highly specific to an antigen of a mycobacterium comprises applying sera from a mammal inoculated with mycobacteria to a first affinity matrix prepared with isolated antigen from mycobacterium such that antibody specific to the isolated antigen is retained by the first affinity matrix; isolating antibody specific to the isolated antigen from the first affinity matrix; applying the isolated antibody to a second affinity matrix prepared with modified antigen from mycobacterium such that antibody specific to the modified antigen is retained by the second affinity matrix, wherein the modified antigen has been treated with an agent to deactivate it relative to the isolated antigen; isolating enriched antibody specific to the isolated antigen by collecting effluent from the second affinity matrix, so that the enriched antibody is more highly specific and displays higher sensitivity to mycobacterium antigen than non-enriched antibody.
- the mycobacterium may be Mycobacterium tuberculosis. and the surface polysaccharide may be lip
- the agent for modifying the antigen from mycobacterium is sodium periodate.
- the surface polysaccharide may be isolated from Freund's adjuvant.
- Still another embodiment provides a method for detecting a mycobacterial infection in a sample from a subject.
- the method comprises providing an immunoreactive environment, such environment developed from the enriched antibody as described above; and reacting the sample in the immunoreactive environment so as to detect the mycobacterial infection.
- the mycobacterial infection may be M. tuberculosis or Johne's disease.
- the surface polysaccharide may be lipoarabinomannan (LAM).
- the immunoreactive environment comprises an ELISA, and may be implemented as a strip test.
- the mycobacterial infection may be a pulmonary Mycobacterium tuberculosis infection or an extra-pulmonary Mycobacterium tuberculosis infection, and the sample may be any of sputum, blood, urine, tissue or other suitable sample.
- the sample may be non-processed unconcentrated urine.
- kits for detecting a mycobacterial infection in a sample comprising an assay providing an immunoreactive environment wherein the environment comprises an enriched antibody as described above.
- the immunoreactive environment comprises an ELISA, and may be implemented as a strip test.
- the mycobacterial infection may be Mycobacterium tuberculosis and the antibody may be lipoarabinomannan (LAM).
- Figure 1 shows a structural model of mycobacterial ManLAM, PILAM, and AraLam.
- Figure 2 shows a comparison of serological activity for LAM experiments.
- Figure 3 shows the efficiency of LAM-specific Ab preparations in capture ELISA.
- Figure 4a Sensitivity of the LAM ELISA for different concentrations of LAM in urine. The cut off was the Optical Density of the Negative Control + 0.1, resulting in a minimal detection limit of 0.25 ng/ml.
- FIG. 4c Sensitivity of the LAM ELISA for various mycobacterial strains. LAM of M. bovis and M. tuberculosis are detected most sensitively.
- FIG. 5 Correlation between the microscopic mycobacterial density of AFB positive patients and their antigen concentration measured by the LAM ELISA in unprocessed urine.
- AFB + (light microscopy 1000 x magnification: 4-90 acid fast bacilli/100 fields) 28 cases.
- AFB ++ (1-9/field) 23 cases.
- AFB +++ ( ⁇ 10/field) 20 cases. Box plot showing 10 th , 25 th , 50 th , 75 th , 90 th percentile and the mean antigen concentration.
- Figure 6 shows a schematic of an antigen purification process in accordance with particular embodiments of the claimed invention.
- Figure 7 shows a schematic for preparing affinity columns in accordance with particular embodiments of the claimed invention.
- FIG. 8 shows a schematic of an antibody purification process in accordance with particular embodiments of the present invention.
- Figure 9 shows a schematic of a conjugate preparation, in accordance with particular embodiments of the present invention.
- Immunoreactive environment means, an environment supportive of immunoassays, immunoreactions, immunochemistry, and any process, assay, methodology or system which involves, relates to or relies on an immunological reaction to achieve a desired result.
- immunoreactive environments are those detailed in US Patent No. 5,073,484 to Swanson et al.; and US Patent Nos. 5,654,162 and 6,020,147 to Guire et al, disclosing method and apparatus for quantitatively determining an analyte in a liquid, wherein particular embodiments employ immunochemical reactions in which the analyte and the reactant represent different parts of a specific ligand (antigen) - antibody (anti-ligand) binding pair.
- M. tuberculosis antigens such as the surface polysaccharides lipoarabinomannan (LAM) and related species
- LAM lipoarabinomannan
- tests of this nature lacked sensitivity and were not operable for unprocessed urine samples or for detecting extrapulmonary TB infections.
- enriched antibodies raised to antigen from mycobacteria wherein the antibody is enriched by having been raised in an environment that maintains antigenically active antigen.
- the method for producing this class of antibodies begins by following the "direct method” to obtain enriched antibodies, but then also operates by excluding antibodies that recognize relatively inactive antigen.
- Figure 8 is a schematic depiction showing the steps involved in practicing an embodiment of the enhanced method. Because the enhanced method builds on the direct method, Figure 8 also illustrates the direct method, if one stops after the first affinity column.
- enriched antibodies of either or both classes can be used to detect pulmonary and extrapulmonary infections of TB in a variety of samples, including but not limited to untreated (i.e. non-concentrated) urine samples. (Other potential sources of sample include sputum, cerebrospinal fluid, blood, tissue, lavages.)
- the enriched antibodies are raised to an epitope of lipoarabinomannan (LAM) in an environment which maintains its antigenic activity.
- LAM lipoarabinomannan
- Embodiments of the present invention overcome difficulties in the prior art by providing enriched antibodies that may be used for detecting mycobacterial antigens in a wide range of sample types from a subject. These sample types include sera, blood, sputum, lavages, tissue, and unprocessed, non-concentrated urine, among others.
- Lipoarabinomannan is a 17500 mol wt lipopolysaccharide specific for the genus mycobacterium. Lipoarabinomannan is a complex polysaccharide antigen composed of mannose and arabinose residues forming a highly branched and complex structure. Despite more than four decades of structural studies of polysaccharide antigens of mycobacteria, those in the art still speak only about fragments of the structure or structural motifs and composite models. The most recent composite model of LAM structure is presented in Fig.l, below. As part of the outer cell wall of mycobacteria, LAM is released from metabolically active or degenerating bacterial cells.
- LAM mannosyl-phospahtidyl-myo-inositol
- Arabinan polysaccharide chains are capped by mannose oligosaccharides, consisiting of mono- ( ⁇ l-2)-di- and ( ⁇ l-2)-tri-mannosyl units variable in their length (capping motifs). Capping degree is variable from strain to strain and possibly is also dependent from growth conditions.
- Fig.3 shows the efficiency of such antibody as a capture antibody.
- HRP horse radish peroxidase
- Phenol extract the cells then ethanol precipitate and place the precipitated cells in the refrigerator (2-8 0 C) overnight (- 16 hours) to allow the precipitate to settle. Being very careful not to disturb precipitate, gently draw off the supernatant until about 100 mL of supernatant is left covering the precipitate. Gently swirl to mix, then transfer the remaining suspension into teflon centrifuge tubes and centrifuge at 12,0Q0rpm for 20 minutes. Draw off as much supernatant as possible from all tubes with out disturbing the pellet, add 5 ml of deionized water to each tube and, using vortexing and pulse sonication, dissolve pellet in water.
- Activation of Sepharose by NaIO 4 Measure an aliquot of suspension of Sepharose 4B-CL corresponding to 80 ml of settled gel and transfer onto a sintered glass filter. Wash with 500 mL water and drain using low vacuum (approx 300 mmHg) until the granular structure of the gel surface becomes visible. Avoid formation of the air cracks in the gel layer. Prepare a 0.1 M sodium acetate buffer, pH 4.0 solution and use to prepare a 30 mM solution of NaIO 4 in 0.1 M NaOAc. Add 250 mL of 30 mM NaIO 4 to the gel and thoroughly mix. Cover the bottle with aluminum foil and place at a 45° angle on a rocker platform at medium speed for 1.5 hours + 10 minutes at ambient temperature. Transfer to the sintered glass filter and wash with 1 L of water using low vacuum (approx 300 mmHg). The activated gel must be prepared within a maximum of 4 hours of use.
- Preparation of matrix Prepare a 0.1% sodium azide solution in IX PBS (phosphate buffered saline). Measure a suspension of activated Sepharose corresponding to 60 ml of the settled gel (or other suitable matrix) and transfer it onto a sintered glass filter. Drain gel using low vacuum (300 mm Hg) until the gel packs and granular structure becomes visible, but avoid formation of cracks on the gel surface.
- IX PBS phosphate buffered saline
- Coupling step To the LAM solution prepared above add the drained activated Sepharose gel. Tightly close and thoroughly mix the suspension using gentle vortexing. Incubate for approx 4 hours at 37°C+/- 2°C, mixing (by inversion) the reaction mixture every hour. Add 4.5 mL of 1.5 M Tris buffer and tightly close cap again. Continue incubating at 37°C +/- 2°C for approximately 16 hours (overnight). Transfer the reaction mixture onto a sintered glass filter and collect the liquid phase into a clean 100 - 200 mL Bunzen flask by applying low vacuum (300mm Hg). Wash the LAM - Sepharose gel on the filter with 400 ml of deionized water and continue washing with 60O mL of IX PBS
- Coupling step To the LAM solution prepared above add the drained activated Sepharose gel. Tightly close with the supplied plastic cap. Thoroughly mix the suspension using gentle vortexing (medium speed) and incubate for approx 4 hours at 37°C ⁇ 2°C, mixing the reaction mixture (by inversion) every hour. Add 7.5mL of 1.5 M Tris buffer and tightly close. Continue incubating at 37°C ⁇ 2°C for approximately 16 hours (overnight).
- CoJurmi Wash Continue to wash the column with IX PBS at a flow rate of 2.0-2.5 mL/min. Pass minimum 3 column volumes of IX PBS. Elute material absorbed onto column with cold 0.1M GIy-HCl buffer, prepared above. Collect material eluted in glass vials. When the monitor/signal drops to -10-15% of baseline, stop collection. Neutralize the collected Antibody solution by adding 10% of total volume of 0.5M sodium phosphate, prepared above, by adding in 0.5 mL increments. Measure the O.D. of antibodies at 280 and calculate the antibody concentration. Immediately place the collected antibodies solution at 4-8 0 C and retain until the analysis of antibodies collected in step above is complete.
- concentration of antibodies above is less than 0.3 mg/mL, concentrate. Wash the column with a minimum of 3 column volumes of IX PBS at a 2.0-2.5 mL/min flow rate. Wash the column again with 1 column volume of IX PBS plus 0.1% sodium azide, and store at 4-8 0 C until future use.
- the Ab coating must be completed within maximum 8 hours from end of preparation of the coating solution M815.
- the Antibody coating solution must be kept in on ice (O 0 C) during the coating process.
- Block Solution must be used within maximum 24 hours from end of preparation
- MTB-LAM-Ab Place the MTB-LAM-Ab (from above) into a V-shaped glass vial with triangular stir bar, without leaving drops of the Ab solution on the vial walls.
- Conjugate Storage and Analysis After dialysis, centrifuge the conjugate solution at 4000 rpm for approx.4 min. Carefully withdraw supernatant and place conjugate solution into the clean 6 ml glass vial. Measure 18 ml of Gardian Peroxidase Conjugate Stabilizer/ Diluent into the 50 ml glass bottle with magnetic stir bar. Add 2 ml of MTB-Ab-HRP conjugate and stir the mixture for approx. 10 min. Store at 2-8°C, and protect from light.
- MTB-ELISA direct antigen sandwich immunoassay
- the test kit consists of an 96-well ELISA plate pre-coated with LAM-specific antibody, blocked and sealed in a plastic pouch with desiccant; a vial with LAM-specific HRP-conjugated LAM-specific polyclonal antibody; a vial with TMB single component chromogenic substrate; a vial with the negative control solution, and three vials with calibrators corresponding to 0.5 ng/ml, 1.5 ng/ml and 4.5 ng/ml of LAM in urinary samples.
- Urine samples were considered positive in the ELISA when the obtained optical density at 450 nm was at least 0.1 above signal of the negative control (>2SD).
- a patient urine sample of 0.1 ml is placed in duplicates on the ELISA plate, incubated for
- the specific isoform of lipoarabinomannan (LAM) determined to contain the antigenic activity is used to generate highly specific, highly pure polyclonal antibodies for use in the detection of mycobacterium lipoarabinomannan in the urine of patients to be screened for active tuberculosis, using protocols similar to that described above.
- the antigenically active isoform of LAM was identified using selective oxidation of LAM, wherein two isoforms were readily identifiable and distinguishable (data not shown). One contained portions sensitive to high concentrations of sodium periodate (NaIO 4 ) such that at high concentrations of sodium periodate the serological activity of the LAM was destroyed. The other isoform maintained serological activity, even when subjected to high concentrations of sodium periodate.
- LAM activated with CNBr or oxidized with mild oxidizing agents or low concentrations of NaIO 4 is used to generate highly antigenic LAM for use in the preparation of highly specific, highly pure polyclonal antibodies for use in detecting LAM in urine samples for diagnosing TB in patients of interest.
- Ziehl Neelson staining and microscopy was done by an experienced and well qualified lab technician. After decontamination sputum samples were cultured on Loewenstein Jenssen medium in duplicates. Cultures were examined weekly for growth for 8 weeks.
- Urine samples of 23 staff members of the Mbeya Referral Hospital, of 20 staff members of Chemogen, Inc. and of 200 patients from 2 clinics in New York were tested in the LAM ELISA. All of them appeared healthy in clinical examination and did not have any signs of respiratory infections.
- Fig. 4a shows the dose response curve using different concentrations of LAM in urine.
- the optimal cut off value was defined according to these curve as LAM concentration producing an optical density (OD) exceeding OD of negative control by 0.1 OD, that corresponds to more than 2 standard deviations above the signal of the negative control sample. All samples with an optical density above this cut off were considered as ELISA positive.
- the cut off was equal to approximately 0.25 ng/ml of LAM in untreated fresh urine.
- the MTB-ELISA was evaluated for cross-reactivity with other species and genera of various Gram-positive and Gram-negative bacteria typical for urinary tract infections and bacterial pneumonia. None of the tested species has shown any reactivity in the evaluated
- the 242 TB suspects were divided into 3 major categories: (1) pulmonary TB patients with confirmed microscopic and/or culture diagnosis, (2) patients with typical clinical and radiographic signs and (3) patients with clinical symptoms of TB, that were not considered TB patients as all available diagnostic tools (radiography, sputum microscopy and culture) were negative.
- Group two comprised an additional 17 patients that were enrolled into the DOTS therapy program based on radiographic and clinical findings (Table 1).
- the 88 patients of group three were sputum negative and did not present specific radiological signs of pulmonary TB and were therefore not enrolled in the DOTS program.
- the mean age of the participants was 34 years.
- the female male ratio was 41:59.
- the overall HW prevalence among the 223 patients that agreed to be tested for HIV was 1069.1 % (see Table 2).
- the HTV prevalence was 73.2% among patients with and 60.8% among patients without confirmed TB.
- the HTV serostatus did not influence the sensitivity of the LAM-ELISA in confirmed pulmonary TB patients.
- 124 patients with known HIV serostatus and positive TB culture and/or AFB stain 73 of 89 HW infected patients (82.0%) were positive in the LAM-ELISA compared to 26 out of 35 uninfected individuals (74.3%).
- the sensitivity of the AFB was not comprised by HW serostatus.
- the sensitivity was 61.2% and 58.8% in HW infected and negative individuals, respectively.
- the specificity of the assay was assessed using the urine of healthy Portugaln and US volunteers. The urine of 23 healthy hospital staff members of Tanzanian origin was analyzed. None of the samples was tested positive in LAM-ELISA (-0.047 mean relative OD, specificity 100%).
- Urine samples of 220 healthy volunteers from US were collected and analyzed. All but 4 had an optical density below the cut off 0.1 (specificity 98.18%).
- the criteria a that were set for such a new assay are a) a higher sensitivity than microscopy, b) comparable specificity, c) a limited additional work load, d) the possibility to diagnose sputum-negative TB and e) a sensitivity that is not impaired by HIV co-infection.
- the sensitivity of the LAM-ELISA (81 % of culture positives) was superior to AFB-stain (69%). Sensitivity can be further improved by concentrating fresh urine, which would however result in an additional effort for a lab technician.
- the specificity of the ELISA was high (98.18% in US and 100% in Africa). HIV co-infection in culture positive TB cases did not influence the sensitivity of the LAM-ELISA. In comparison to previous published results of the LAM-ELISA the new test detects
- TB suspected patients remained ambiguous in terms of their TB status (group 2 and 3).
- Group 1 laboratory confirmed TB
- Group 2 clinically and radiological diagnosed TB
- Group 3 no laboratory or radiological proof of TB. While we are confident that participants in category 1 are true TB cases, we cannot exclude that category 2 and 3 contain some wrongly categorized patients. We therefore excluded them from our sensitivity and specificity calculation. Diagnosis of TB often requires the longitudinal follow-up of patients. Especially sputum negative patients with unusual radiological features would have needed several follow-up consultations in order to re-question their TB status. In a longitudinal study clinical as well as diagnostic reevaluation and TB treatment outcome would have given important additional information to classify group 2 and 3 in TB and non TB patients.
- the combination with a the capacity to detect extrapulmonary and AFB-negative TB renders the LAM assay potent tool in an environment with a growing prevalence of extrapulmonary forms of TB and pulmonary forms with atypical clinical symptoms.
- the LAM-ELISA could not only be used for the diagnosis of patients with clinical symptoms, but also for screening HIV positive patients and other high risk groups.
- Early case detection of active TB and effective treatment are the two pillars in a successful fight against TB. To further explore the role of the LAM assay in this fight we are currently planning several prospective and multicenter studies.
- the LAM-ELISA can be easily integrated in the routine diagnostic procedures of laboratories of both, developed and developing countries. It is an easy to use and robust assay. Completion of the ELISA requires only 2 1 A hr and many samples can be analyzed at the same time. As the antigen Lipoarabinomannan is stable, it was possible to keep the urine refrigerated for 3 days without significant drop in optical density. The newly developed MTB-ELISA for detection of LAM in unprocessed urine has the potential of a screening test to be used also under field conditions in developing countries.
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Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
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JP2007522751A JP4948402B2 (en) | 2004-07-20 | 2005-07-20 | Concentrated antibody for detecting mycobacterial infection, method of use, and diagnostic test using the same |
EA200700345A EA013228B1 (en) | 2004-07-20 | 2005-07-20 | A process for producing an enriched polyclonal antibody highly specific to an antigen of a surface polysaccharide from lipoarabinomannan microbacteria and method of use thereof |
AP2007003884A AP2316A (en) | 2004-07-20 | 2005-07-20 | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same. |
AT05791349T ATE500272T1 (en) | 2004-07-20 | 2005-07-20 | ENRICHED ANTIBODIES FOR DETECTING MICROBACTERIAL INFECTION, METHOD OF USE AND DIAGNOSTIC TEST THEREOF |
DE602005026665T DE602005026665D1 (en) | 2004-07-20 | 2005-07-20 | ENRICHED ANTIBODY FOR THE DETECTION OF MICROBACTERIAL INFECTION, METHOD FOR THE USE AND DIAGNOSTIC TEST THEREWITH |
AU2005267111A AU2005267111B2 (en) | 2004-07-20 | 2005-07-20 | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same |
MX2007000916A MX2007000916A (en) | 2004-07-20 | 2005-07-20 | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same. |
CA2574432A CA2574432C (en) | 2004-07-20 | 2005-07-20 | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same |
CN200580029939XA CN101014622B (en) | 2004-07-20 | 2005-07-20 | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same |
EP05791349A EP1771475B1 (en) | 2004-07-20 | 2005-07-20 | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same |
BRPI0513596A BRPI0513596B8 (en) | 2004-07-20 | 2005-07-20 | method and kit for detecting a mycobacterial infection in a sample from an individual of interest by detecting mycobacterial antigen |
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WO2008067497A2 (en) * | 2006-11-29 | 2008-06-05 | Chemogen, Inc. | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same |
US7615222B2 (en) | 2004-07-20 | 2009-11-10 | Chemogen, Inc. | Enriched antibody for detecting mycobacterial infection, methods of use and diagnostic test employing same |
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US20100317037A1 (en) * | 2009-06-16 | 2010-12-16 | Sina Biotechnical Co. | Detection of Mycobacterium Tuberculosis Bacilli |
WO2012102679A1 (en) | 2011-01-24 | 2012-08-02 | National University Of Singapore | Pathogenic mycobacteria-derived mannose-capped lipoarabinomannan antigen binding proteins |
EP3414573B1 (en) | 2016-02-10 | 2023-11-08 | Rutgers, the State University of New Jersey | Novel anti-lam antibodies |
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CA2574432A1 (en) | 2006-02-02 |
ATE500272T1 (en) | 2011-03-15 |
BRPI0513596B1 (en) | 2018-02-06 |
JP4948402B2 (en) | 2012-06-06 |
EA013228B1 (en) | 2010-04-30 |
CN101014622B (en) | 2011-12-28 |
US20100210823A1 (en) | 2010-08-19 |
AU2005267111A1 (en) | 2006-02-02 |
CA2574432C (en) | 2015-05-26 |
CN101014622A (en) | 2007-08-08 |
EP1771475A1 (en) | 2007-04-11 |
BRPI0513596A (en) | 2008-05-13 |
AP2316A (en) | 2011-11-04 |
US8057797B2 (en) | 2011-11-15 |
JP2008507544A (en) | 2008-03-13 |
MX2007000916A (en) | 2007-07-09 |
UA92721C2 (en) | 2010-12-10 |
AP2007003884A0 (en) | 2007-02-28 |
AU2005267111B2 (en) | 2011-03-03 |
EP1771475B1 (en) | 2011-03-02 |
US20060127406A1 (en) | 2006-06-15 |
US7615222B2 (en) | 2009-11-10 |
DE602005026665D1 (en) | 2011-04-14 |
ZA200700525B (en) | 2008-09-25 |
US20080213806A1 (en) | 2008-09-04 |
EA200700345A1 (en) | 2007-08-31 |
US7335480B2 (en) | 2008-02-26 |
BRPI0513596B8 (en) | 2021-07-27 |
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