WO2006015389A3 - Methods and compositions for enhancing the efficacy and specificity of rna silencing - Google Patents

Methods and compositions for enhancing the efficacy and specificity of rna silencing Download PDF

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Publication number
WO2006015389A3
WO2006015389A3 PCT/US2005/029011 US2005029011W WO2006015389A3 WO 2006015389 A3 WO2006015389 A3 WO 2006015389A3 US 2005029011 W US2005029011 W US 2005029011W WO 2006015389 A3 WO2006015389 A3 WO 2006015389A3
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Prior art keywords
efficacy
specificity
methods
enhancing
rna silencing
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PCT/US2005/029011
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French (fr)
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WO2006015389A2 (en
Inventor
Phillip D Zamore
Gyorgy Hutvagner
Dianne Schwarz
Martin Simard
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Univ Massachusetts
Phillip D Zamore
Gyorgy Hutvagner
Dianne Schwarz
Martin Simard
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Application filed by Univ Massachusetts, Phillip D Zamore, Gyorgy Hutvagner, Dianne Schwarz, Martin Simard filed Critical Univ Massachusetts
Publication of WO2006015389A2 publication Critical patent/WO2006015389A2/en
Publication of WO2006015389A3 publication Critical patent/WO2006015389A3/en

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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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Abstract

The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.
PCT/US2005/029011 2004-08-04 2005-08-04 Methods and compositions for enhancing the efficacy and specificity of rna silencing WO2006015389A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/912,440 US7750144B2 (en) 2003-06-02 2004-08-04 Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US10/912,440 2004-08-04

Publications (2)

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WO2006015389A2 WO2006015389A2 (en) 2006-02-09
WO2006015389A3 true WO2006015389A3 (en) 2006-06-15

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PCT/US2005/029011 WO2006015389A2 (en) 2004-08-04 2005-08-04 Methods and compositions for enhancing the efficacy and specificity of rna silencing

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US (7) US7750144B2 (en)
WO (1) WO2006015389A2 (en)

Cited By (2)

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US8090542B2 (en) 2002-11-14 2012-01-03 Dharmacon Inc. Functional and hyperfunctional siRNA
US9228186B2 (en) 2002-11-14 2016-01-05 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality

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