WO2006051303A8 - Assay for measuring an enzyme’s capability to modify supercoil topology of nucleic acids and modulators - Google Patents
Assay for measuring an enzyme’s capability to modify supercoil topology of nucleic acids and modulatorsInfo
- Publication number
- WO2006051303A8 WO2006051303A8 PCT/GB2005/004346 GB2005004346W WO2006051303A8 WO 2006051303 A8 WO2006051303 A8 WO 2006051303A8 GB 2005004346 W GB2005004346 W GB 2005004346W WO 2006051303 A8 WO2006051303 A8 WO 2006051303A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- enzyme
- target nucleic
- topology
- modulators
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 12
- 102000039446 nucleic acids Human genes 0.000 title abstract 12
- 108020004707 nucleic acids Proteins 0.000 title abstract 12
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 238000003556 assay Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 239000007787 solid Substances 0.000 abstract 3
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
Abstract
Provided are methods, such as high throughput methods, of assessing or measuring the ability of an enzyme to modify the topology (e.g. supercoil topology) of a target nucleic acid, the methods comprising the steps of: (a) providing a solid support to which a capture nucleic acid is or may be immobilised, which capture nucleic acid is capable of binding the target nucleic acid in a manner that is proportional to the supercoil topology of said target nucleic acid (e.g. by triplex formation); (b) incubating a test mixture comprising (i) the enzyme, (ii) the target nucleic acid, (iii) capture nucleic acid, in the presence of (iv) said solid support, such that supercoiled target nucleic acid bound by the capture nucleic acid is immobilised to the solid support, (c) determining the amount of target nucleic acid bound by said capture nucleic acid in step (b) e.g. by use of a detectable label. The method may be used to screen for modulators of the enzyme activity.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE602005018808T DE602005018808D1 (en) | 2004-11-11 | 2005-11-10 | ODIFICATION OF SUPERCOIL TOPOLOGY OF NUCLEIC ACIDS AND MODULATORS |
| AT05803594T ATE454475T1 (en) | 2004-11-11 | 2005-11-10 | DEVICE FOR MEASURING THE ABILITY OF AN ENZYME TO MODIFY THE SUPERCOIL TOPOLOGY OF NUCLEIC ACIDS AND MODULATORS |
| CA002586534A CA2586534A1 (en) | 2004-11-11 | 2005-11-10 | Assay for measuring an enzyme's capability to modify supercoil topology of nucleic acids and modulators |
| AU2005303599A AU2005303599A1 (en) | 2004-11-11 | 2005-11-10 | Assay for measuring an enzyme's capability to modify supercoil topology of nucleic acids and modulators |
| EP05803594A EP1812592B1 (en) | 2004-11-11 | 2005-11-10 | Assay for measuring an enzyme's capability to modify supercoil topology of nucleic acids and modulators |
| US11/718,978 US7838230B2 (en) | 2004-11-11 | 2005-11-10 | Assay for measuring an enzyme's capability to modify supercoil topology of nucleic acids and modulators |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0424953A GB0424953D0 (en) | 2004-11-11 | 2004-11-11 | Assay |
| GB0424953.8 | 2004-11-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2006051303A1 WO2006051303A1 (en) | 2006-05-18 |
| WO2006051303A8 true WO2006051303A8 (en) | 2007-07-26 |
Family
ID=33523584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/GB2005/004346 WO2006051303A1 (en) | 2004-11-11 | 2005-11-10 | Assay for measuring an enzyme’s capability to modify supercoil topology of nucleic acids and modulators |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7838230B2 (en) |
| EP (1) | EP1812592B1 (en) |
| AT (1) | ATE454475T1 (en) |
| AU (1) | AU2005303599A1 (en) |
| CA (1) | CA2586534A1 (en) |
| DE (1) | DE602005018808D1 (en) |
| GB (1) | GB0424953D0 (en) |
| WO (1) | WO2006051303A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009037510A1 (en) * | 2007-09-21 | 2009-03-26 | Astrazeneca Ab | Dna decatenation assay 997 |
| WO2009037511A1 (en) * | 2007-09-21 | 2009-03-26 | Astrazeneca Ab | Assay for dna supercoiling/relaxation |
| WO2010044101A1 (en) * | 2008-10-08 | 2010-04-22 | V. B. Medicare Pvt. Ltd. | Purification and assay for topoisomerases and use of the assay for screening modulators of topoisomerases |
| SG11202012163VA (en) * | 2018-06-07 | 2021-01-28 | Amgen Inc | Detection assay for protein-polynucleotide conjugates |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0231955B2 (en) | 1982-12-07 | 1990-07-17 | Rikagaku Kenkyusho | KODONOCHORASENOJUSURUHEIKANJODNANOCHOSEIHO |
| AU1377099A (en) | 1997-11-03 | 1999-05-24 | Small Molecule Therapeutics, Inc. | Methods and compositions for modulation of bacterial topoisomerase enzymes |
| DE19753182A1 (en) | 1997-11-21 | 1999-07-29 | Claas Junghans | Topologically fixed matrix-bound nucleic acid |
| US5998152A (en) | 1998-03-09 | 1999-12-07 | Tularik Inc. | High-throughput screening assays for modulators of nucleic acid topoisomerases |
| EP1086242A1 (en) | 1998-03-25 | 2001-03-28 | The Procter & Gamble Company | Fluorescent assay for topoisomerase inhibitors |
| US6420115B1 (en) | 1999-12-21 | 2002-07-16 | Ingeneus Corporation | Cation mediated triplex hybridization assay |
| US6620588B1 (en) | 2000-05-31 | 2003-09-16 | Salk Institute For Biological Studies | Method of identifying inhibitors of topoisomerase DNA religation |
| WO2001098540A2 (en) | 2000-06-22 | 2001-12-27 | San Diego State University Foundation | Recombination modulators and methods for their production and use |
| AU2002337660A1 (en) | 2001-07-31 | 2003-02-17 | Claude Gagna | Methods for immobilizing molecules to a solid phase and uses thereof |
| US20040022764A1 (en) * | 2002-07-31 | 2004-02-05 | Hanan Polansky | Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease |
| FR2852606A1 (en) | 2003-03-18 | 2004-09-24 | Inst Nat Sante Rech Med | In vitro method for simultaneous inhibition of several genes, useful for treating cancer or viral infection, using a ligand that binds to a common site in the genes and induces cutting by topoisomerase |
| US20070298413A1 (en) | 2003-03-31 | 2007-12-27 | Astrazeneca Ab | Topoisomerase Modulators Assays |
-
2004
- 2004-11-11 GB GB0424953A patent/GB0424953D0/en not_active Ceased
-
2005
- 2005-11-10 AT AT05803594T patent/ATE454475T1/en not_active IP Right Cessation
- 2005-11-10 US US11/718,978 patent/US7838230B2/en not_active Expired - Fee Related
- 2005-11-10 EP EP05803594A patent/EP1812592B1/en not_active Not-in-force
- 2005-11-10 AU AU2005303599A patent/AU2005303599A1/en not_active Abandoned
- 2005-11-10 DE DE602005018808T patent/DE602005018808D1/en active Active
- 2005-11-10 WO PCT/GB2005/004346 patent/WO2006051303A1/en active Application Filing
- 2005-11-10 CA CA002586534A patent/CA2586534A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1812592B1 (en) | 2010-01-06 |
| ATE454475T1 (en) | 2010-01-15 |
| DE602005018808D1 (en) | 2010-02-25 |
| GB0424953D0 (en) | 2004-12-15 |
| US20080026388A1 (en) | 2008-01-31 |
| US7838230B2 (en) | 2010-11-23 |
| AU2005303599A1 (en) | 2006-05-18 |
| EP1812592A1 (en) | 2007-08-01 |
| WO2006051303A1 (en) | 2006-05-18 |
| CA2586534A1 (en) | 2006-05-18 |
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