WO2006076617A3 - Method for classifying a microorganism in a biological sample - Google Patents

Method for classifying a microorganism in a biological sample Download PDF

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Publication number
WO2006076617A3
WO2006076617A3 PCT/US2006/001305 US2006001305W WO2006076617A3 WO 2006076617 A3 WO2006076617 A3 WO 2006076617A3 US 2006001305 W US2006001305 W US 2006001305W WO 2006076617 A3 WO2006076617 A3 WO 2006076617A3
Authority
WO
WIPO (PCT)
Prior art keywords
property
gram
microorganism
gram negative
bacteria
Prior art date
Application number
PCT/US2006/001305
Other languages
French (fr)
Other versions
WO2006076617A2 (en
Inventor
Norman Wainwright
Dana Nutter
Original Assignee
Charles River Lab Inc
Norman Wainwright
Dana Nutter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Charles River Lab Inc, Norman Wainwright, Dana Nutter filed Critical Charles River Lab Inc
Priority to EP06733698A priority Critical patent/EP1836492B1/en
Priority to DE602006004553T priority patent/DE602006004553D1/en
Priority to JP2007551414A priority patent/JP5096166B2/en
Publication of WO2006076617A2 publication Critical patent/WO2006076617A2/en
Publication of WO2006076617A3 publication Critical patent/WO2006076617A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

The invention provides a method using a hemocyte preparation, for example, Limulus amebocyte lysate, for detecting in a single assay the presence of at least one of a Gram negative bacterium, a Gram positive bacterium, and a fungus in a sample of interest. The method exploits the differential reactivity of Gram negative bacteria, Gram positive bacteria, and fungi with the hemocyte preparation to produce measurable changes in a property, for example, an optical property, of the mixture. Because Jhe Gram negative bacteria, Gram positive bacteria and fungi each produce different changes in a given property, for example, an optical property, it is possible to classify the type of microorganism present in the sample of interest.
PCT/US2006/001305 2005-01-13 2006-01-13 Method for classifying a microorganism in a biological sample WO2006076617A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP06733698A EP1836492B1 (en) 2005-01-13 2006-01-13 Method for classifying a microorganism in a biological sample
DE602006004553T DE602006004553D1 (en) 2005-01-13 2006-01-13 METHOD FOR CLASSIFYING MICROBES IN A BIOLOGICAL SAMPLE
JP2007551414A JP5096166B2 (en) 2005-01-13 2006-01-13 Method for classifying microorganisms in biological samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64369705P 2005-01-13 2005-01-13
US60/643,697 2005-01-13

Publications (2)

Publication Number Publication Date
WO2006076617A2 WO2006076617A2 (en) 2006-07-20
WO2006076617A3 true WO2006076617A3 (en) 2006-12-28

Family

ID=36649798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/001305 WO2006076617A2 (en) 2005-01-13 2006-01-13 Method for classifying a microorganism in a biological sample

Country Status (6)

Country Link
US (2) US7479375B2 (en)
EP (1) EP1836492B1 (en)
JP (2) JP5096166B2 (en)
AT (1) ATE419526T1 (en)
DE (1) DE602006004553D1 (en)
WO (1) WO2006076617A2 (en)

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JP5118849B2 (en) * 2003-03-17 2013-01-16 チャールズ リバー ラボラトリーズ， インコーポレイテッド Methods and compositions for detection of microbial contaminants
JP2008522640A (en) 2004-12-02 2008-07-03 チャールズ リバー ラボラトリーズ， インコーポレイテッド Methods and compositions for detection and / or quantification of Gram positive bacteria contaminants
US7479375B2 (en) 2005-01-13 2009-01-20 Charles River Laboratories, Inc. Method for classifying a microorganism in a biological sample
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US8937287B2 (en) 2012-04-19 2015-01-20 Abt Molecular Imaging, Inc. Self-referencing radiation detector for use with a radiopharmaceutical quality control testing system
EP3257947B1 (en) * 2016-06-16 2018-12-19 Biomérieux Method and system for identifying the gram type of a bacterium
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Also Published As

Publication number Publication date
WO2006076617A2 (en) 2006-07-20
DE602006004553D1 (en) 2009-02-12
US7479375B2 (en) 2009-01-20
EP1836492B1 (en) 2008-12-31
JP2008526257A (en) 2008-07-24
ATE419526T1 (en) 2009-01-15
EP1836492A2 (en) 2007-09-26
JP5096166B2 (en) 2012-12-12
JP2012068266A (en) 2012-04-05
US20060216780A1 (en) 2006-09-28
US7901899B1 (en) 2011-03-08

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