WO2007001358A3 - Fluorescence polarization assays for determining clostridial toxin activity - Google Patents
Fluorescence polarization assays for determining clostridial toxin activity Download PDFInfo
- Publication number
- WO2007001358A3 WO2007001358A3 PCT/US2005/032328 US2005032328W WO2007001358A3 WO 2007001358 A3 WO2007001358 A3 WO 2007001358A3 US 2005032328 W US2005032328 W US 2005032328W WO 2007001358 A3 WO2007001358 A3 WO 2007001358A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- clostridial toxin
- fluorescence polarization
- activity
- fluorophore
- determining
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
Abstract
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007533521A JP2008513038A (en) | 2004-09-22 | 2005-09-09 | Fluorescence polarization assay for measurement of clostridial toxin activity |
EP05858107A EP1807698B1 (en) | 2004-09-22 | 2005-09-09 | Assay for determining clostridial toxin activity combining fret and fluorescence polarization |
CA002581231A CA2581231A1 (en) | 2004-09-22 | 2005-09-09 | Fluorescence polarization assays for determining clostridial toxin activity |
AU2005333632A AU2005333632B2 (en) | 2004-09-22 | 2005-09-09 | Fluorescence polarization assays for determining clostridial toxin activity |
KR1020077009002A KR101442246B1 (en) | 2004-09-22 | 2005-09-09 | Fluorescence Polarization Assays for Determining Clostridial Toxin Activity |
DE602005021981T DE602005021981D1 (en) | 2004-09-22 | 2005-09-09 | Assay for determining the activity of clostridial toxin by combining FRET and fluorescence polarization |
AT05858107T ATE472101T1 (en) | 2004-09-22 | 2005-09-09 | ASSAY TO DETERMINE CLOSTRIDIAL TOXIN ACTIVITY USING A COMBINATION OF FRET AND FLUORESCENCE POLARIZATION |
DK05858107.5T DK1807698T3 (en) | 2004-09-22 | 2005-09-09 | Assay to determine the activity of clostridial toxin by combination of FRET and fluorescence polarization |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/948,097 | 2004-09-22 | ||
US10/948,097 US7399607B2 (en) | 2004-09-22 | 2004-09-22 | Fluorescence polarization assays for determining clostridial toxin activity |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007001358A2 WO2007001358A2 (en) | 2007-01-04 |
WO2007001358A3 true WO2007001358A3 (en) | 2007-06-28 |
Family
ID=36074524
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/032328 WO2007001358A2 (en) | 2004-09-22 | 2005-09-09 | Fluorescence polarization assays for determining clostridial toxin activity |
Country Status (11)
Country | Link |
---|---|
US (7) | US7399607B2 (en) |
EP (2) | EP1807698B1 (en) |
JP (2) | JP2008513038A (en) |
KR (1) | KR101442246B1 (en) |
AT (1) | ATE472101T1 (en) |
AU (1) | AU2005333632B2 (en) |
CA (1) | CA2581231A1 (en) |
DE (1) | DE602005021981D1 (en) |
DK (1) | DK1807698T3 (en) |
ES (2) | ES2345780T3 (en) |
WO (1) | WO2007001358A2 (en) |
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US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
AU2006340711C1 (en) * | 2005-04-05 | 2013-02-07 | Allergan, Inc. | Clostridial toxin activity assays |
ATE470857T1 (en) * | 2005-04-05 | 2010-06-15 | Allergan Inc | LIPOPHILIC, DYE-BASED FRET ASSAYS FOR CLOSTRIDIAL TOXINACTIVITY |
WO2008008082A2 (en) * | 2005-09-19 | 2008-01-17 | Allergan, Inc. | Clostridial toxin activatable clostridial toxins |
EP1934610B1 (en) * | 2005-10-12 | 2010-01-27 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
US8003314B2 (en) | 2007-04-16 | 2011-08-23 | Diagnostic Hybrids, Inc. | Methods for direct fluorescent antibody virus detection in liquids |
US8753831B2 (en) * | 2007-06-05 | 2014-06-17 | City Of Hope | Methods for detection of botulinum neurotoxin |
KR101087017B1 (en) * | 2007-07-10 | 2011-12-09 | (주)메디톡스 | Stable liquid composition of Botulinum Toxin |
KR101604515B1 (en) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | Immuno-Based Botulinum Toxin Serotype A Activity Assays |
CN102356091A (en) | 2008-03-14 | 2012-02-15 | 阿勒根公司 | Immuno-based botulinum toxin serotype a activity assays |
SI2406371T1 (en) * | 2009-03-13 | 2018-10-30 | Allergan, Inc. | Cells useful for immuno-based botulinum toxin serotype a activity assays |
DK3281953T3 (en) * | 2009-03-13 | 2020-01-20 | Allergan Inc | IMMUN-BASED REDIRECT ENDOPEPTIDASE ACTIVITY ASSAYS |
US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
WO2011041779A1 (en) | 2009-10-02 | 2011-04-07 | Allergan, Inc. | In vitro method of determining changes in a protein environment |
US10451621B2 (en) | 2011-12-31 | 2019-10-22 | Allergan, Inc. | Highly sensitive cell-based assay to detect the presence of active botulinum neurotoxin serotype-A |
LT2800973T (en) | 2012-01-04 | 2018-09-10 | Biomadison, Inc. | Methods and compounds for increasing sensitivity of botulinum assays |
US8962340B2 (en) | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
LT3030905T (en) | 2013-08-09 | 2018-01-25 | Biomadison, Inc. | Botulinum toxin assay with improved sensitivity |
EP3166971B1 (en) | 2014-07-07 | 2019-09-11 | Allergan, Inc. | Method of detecting cleaved snap25 in tissue samples |
FR3053689B1 (en) * | 2016-07-06 | 2021-01-08 | Univ Claude Bernard Lyon | POLYPEPTIDES PROTEASE SUBSTRATES OF THE ASTACIN FAMILY AND THEIR USES |
US10369190B2 (en) | 2016-09-13 | 2019-08-06 | Allergan, Inc. | Non-protein clostridial toxin compositions |
EP3529371B1 (en) * | 2016-10-21 | 2022-06-22 | Toxogen GmbH | A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
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WO2003020948A2 (en) * | 2001-08-28 | 2003-03-13 | Allergan, Inc. | Fret protease assays for botulinum serotype a/e toxins |
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-
2004
- 2004-09-22 US US10/948,097 patent/US7399607B2/en active Active
-
2005
- 2005-09-09 WO PCT/US2005/032328 patent/WO2007001358A2/en active Application Filing
- 2005-09-09 EP EP05858107A patent/EP1807698B1/en not_active Revoked
- 2005-09-09 JP JP2007533521A patent/JP2008513038A/en active Pending
- 2005-09-09 AT AT05858107T patent/ATE472101T1/en not_active IP Right Cessation
- 2005-09-09 DE DE602005021981T patent/DE602005021981D1/en active Active
- 2005-09-09 KR KR1020077009002A patent/KR101442246B1/en active IP Right Grant
- 2005-09-09 ES ES05858107T patent/ES2345780T3/en active Active
- 2005-09-09 DK DK05858107.5T patent/DK1807698T3/en active
- 2005-09-09 EP EP09160607A patent/EP2107112A1/en not_active Withdrawn
- 2005-09-09 AU AU2005333632A patent/AU2005333632B2/en not_active Ceased
- 2005-09-09 CA CA002581231A patent/CA2581231A1/en not_active Abandoned
-
2008
- 2008-05-21 US US12/124,412 patent/US7638294B2/en active Active
- 2008-05-22 US US12/125,723 patent/US7632655B2/en active Active
- 2008-05-22 US US12/125,235 patent/US7635574B2/en active Active
- 2008-05-22 US US12/125,327 patent/US7674601B2/en active Active
-
2009
- 2009-04-23 ES ES21203994T patent/ES2928863T3/en active Active
- 2009-10-22 US US12/604,059 patent/US20100081155A1/en not_active Abandoned
- 2009-10-22 US US12/604,138 patent/US20100081156A1/en not_active Abandoned
-
2012
- 2012-05-31 JP JP2012125033A patent/JP2012210215A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20010046668A1 (en) * | 1994-10-21 | 2001-11-29 | G.D. Searle & Co. | Fluorescence polarization method for determining protease activity |
WO2002025284A2 (en) * | 2000-09-25 | 2002-03-28 | U.S. Medical Research Institute Of Infectious Diseases | High-throughput assays for the proteolytic activities of clostridial neurotoxins |
WO2003020948A2 (en) * | 2001-08-28 | 2003-03-13 | Allergan, Inc. | Fret protease assays for botulinum serotype a/e toxins |
Also Published As
Publication number | Publication date |
---|---|
KR101442246B1 (en) | 2014-10-01 |
JP2008513038A (en) | 2008-05-01 |
US20060063222A1 (en) | 2006-03-23 |
ES2345780T3 (en) | 2010-10-01 |
US20080305509A1 (en) | 2008-12-11 |
US20100081156A1 (en) | 2010-04-01 |
EP2107112A1 (en) | 2009-10-07 |
US7399607B2 (en) | 2008-07-15 |
US20080293085A1 (en) | 2008-11-27 |
AU2005333632A1 (en) | 2007-01-04 |
DK1807698T3 (en) | 2010-09-20 |
EP1807698B1 (en) | 2010-06-23 |
EP1807698A2 (en) | 2007-07-18 |
JP2012210215A (en) | 2012-11-01 |
US7638294B2 (en) | 2009-12-29 |
US20080305510A1 (en) | 2008-12-11 |
DE602005021981D1 (en) | 2010-08-05 |
ES2928863T3 (en) | 2022-11-23 |
AU2005333632B2 (en) | 2011-12-22 |
KR20070055609A (en) | 2007-05-30 |
US20080293084A1 (en) | 2008-11-27 |
US7674601B2 (en) | 2010-03-09 |
ATE472101T1 (en) | 2010-07-15 |
CA2581231A1 (en) | 2007-01-04 |
WO2007001358A2 (en) | 2007-01-04 |
US20100081155A1 (en) | 2010-04-01 |
US7635574B2 (en) | 2009-12-22 |
US7632655B2 (en) | 2009-12-15 |
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