WO2007020457A3 - Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use - Google Patents

Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use Download PDF

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Publication number
WO2007020457A3
WO2007020457A3 PCT/GB2006/003095 GB2006003095W WO2007020457A3 WO 2007020457 A3 WO2007020457 A3 WO 2007020457A3 GB 2006003095 W GB2006003095 W GB 2006003095W WO 2007020457 A3 WO2007020457 A3 WO 2007020457A3
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WIPO (PCT)
Prior art keywords
nucleotides
methods
group
polyethylene glycol
base
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PCT/GB2006/003095
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French (fr)
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WO2007020457A2 (en
Inventor
Xiaohai Liu
John Milton
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Solexa Ltd
Xiaohai Liu
John Milton
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Application filed by Solexa Ltd, Xiaohai Liu, John Milton filed Critical Solexa Ltd
Priority to EP06779167.3A priority Critical patent/EP1926829B2/en
Publication of WO2007020457A2 publication Critical patent/WO2007020457A2/en
Publication of WO2007020457A3 publication Critical patent/WO2007020457A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Modified guanine-containing nucleosides and nucleotides, in particular fluorescently labelled guanine-containing nucleosides and nucleotides, which exhibit reduced quenching effects, and hence enhanced brightness of the fluorophore are described.
PCT/GB2006/003095 2005-08-19 2006-08-18 Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use WO2007020457A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06779167.3A EP1926829B2 (en) 2005-08-19 2006-08-18 Labelled modified guanine-containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0517097.2 2005-08-19
GBGB0517097.2A GB0517097D0 (en) 2005-08-19 2005-08-19 Modified nucleosides and nucleotides and uses thereof

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WO2007020457A2 WO2007020457A2 (en) 2007-02-22
WO2007020457A3 true WO2007020457A3 (en) 2007-10-25

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US (3) US7592435B2 (en)
EP (1) EP1926829B2 (en)
GB (1) GB0517097D0 (en)
WO (1) WO2007020457A2 (en)

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US8212015B2 (en) 2012-07-03
EP1926829A2 (en) 2008-06-04
US7816503B2 (en) 2010-10-19
WO2007020457A2 (en) 2007-02-22
US20110020827A1 (en) 2011-01-27
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US7592435B2 (en) 2009-09-22

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