WO2007097816A3 - Time-lapse cell cycle analysis of unstained nuclei - Google Patents

Time-lapse cell cycle analysis of unstained nuclei Download PDF

Info

Publication number
WO2007097816A3
WO2007097816A3 PCT/US2006/060646 US2006060646W WO2007097816A3 WO 2007097816 A3 WO2007097816 A3 WO 2007097816A3 US 2006060646 W US2006060646 W US 2006060646W WO 2007097816 A3 WO2007097816 A3 WO 2007097816A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell cycle
cell
time
cycle analysis
progression
Prior art date
Application number
PCT/US2006/060646
Other languages
French (fr)
Other versions
WO2007097816A2 (en
Inventor
Jens Rittscher
Dirk R Padfield
Thomas Sebastian
Nicholas Thomas
Original Assignee
Gen Electric
Jens Rittscher
Dirk R Padfield
Thomas Sebastian
Nicholas Thomas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric, Jens Rittscher, Dirk R Padfield, Thomas Sebastian, Nicholas Thomas filed Critical Gen Electric
Priority to EP06850118A priority Critical patent/EP1946270B1/en
Priority to AU2006338566A priority patent/AU2006338566B2/en
Priority to DK06850118.8T priority patent/DK1946270T3/en
Priority to CA2629349A priority patent/CA2629349C/en
Priority to ES06850118T priority patent/ES2373689T3/en
Priority to PL06850118T priority patent/PL1946270T3/en
Priority to JP2008540324A priority patent/JP5069245B2/en
Priority to AT06850118T priority patent/ATE532154T1/en
Publication of WO2007097816A2 publication Critical patent/WO2007097816A2/en
Publication of WO2007097816A3 publication Critical patent/WO2007097816A3/en

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/20—Analysis of motion
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/12—Edge-based segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/149—Segmentation; Edge detection involving deformable models, e.g. active contour models
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/174—Segmentation; Edge detection involving the use of two or more images
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10016—Video; Image sequence
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20112—Image segmentation details
    • G06T2207/20156—Automatic seed setting
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20112—Image segmentation details
    • G06T2207/20161—Level set
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Abstract

The present invention provides a cell imaging technique for automatically tracking the progression of a cell through the cell cycle over time through segmentation of a volume of two-dimensional time-lapse images. The technique allows long-term tracking of the cell cycle progression of an individual cell or multiple cells. Further, the invention provides a unique display of cell cycle progression, allowing an end user to easily determine changes to cell cycle progression for a cell of interest.
PCT/US2006/060646 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei WO2007097816A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP06850118A EP1946270B1 (en) 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei
AU2006338566A AU2006338566B2 (en) 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei
DK06850118.8T DK1946270T3 (en) 2005-11-12 2006-11-08 Spatial core cell cycle analysis
CA2629349A CA2629349C (en) 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei
ES06850118T ES2373689T3 (en) 2005-11-12 2006-11-08 ANALYSIS OF TEMPORARY LAPSES OF THE CELL PHONE OF NON-DYNATED CORES.
PL06850118T PL1946270T3 (en) 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei
JP2008540324A JP5069245B2 (en) 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei
AT06850118T ATE532154T1 (en) 2005-11-12 2006-11-08 TIME SPACE CELL CYCLE ANALYSIS OF UNSPOTTED NUCLEARS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73634505P 2005-11-12 2005-11-12
US60/736,345 2005-11-12

Publications (2)

Publication Number Publication Date
WO2007097816A2 WO2007097816A2 (en) 2007-08-30
WO2007097816A3 true WO2007097816A3 (en) 2008-01-10

Family

ID=38437827

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/060646 WO2007097816A2 (en) 2005-11-12 2006-11-08 Time-lapse cell cycle analysis of unstained nuclei

Country Status (10)

Country Link
US (1) US7817841B2 (en)
EP (1) EP1946270B1 (en)
JP (1) JP5069245B2 (en)
AT (1) ATE532154T1 (en)
AU (1) AU2006338566B2 (en)
CA (1) CA2629349C (en)
DK (1) DK1946270T3 (en)
ES (1) ES2373689T3 (en)
PL (1) PL1946270T3 (en)
WO (1) WO2007097816A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4973162B2 (en) * 2006-12-01 2012-07-11 株式会社ニコン Image processing apparatus, image processing program, and observation system
US20100195868A1 (en) * 2007-05-31 2010-08-05 Lu Peter J Target-locking acquisition with real-time confocal (tarc) microscopy
US7940978B2 (en) * 2007-06-05 2011-05-10 General Electric Company Automatic characterization of cellular motion
US8411919B2 (en) * 2008-07-07 2013-04-02 Siemens Aktiengesellschaft Fluid dynamics approach to image segmentation
EP2193468B1 (en) * 2007-09-26 2019-03-27 Massachusetts Institute of Technology High-resolution 3d imaging of single semiconductor nanocrystals
JP5762764B2 (en) * 2011-02-09 2015-08-12 オリンパス株式会社 Cell image analysis system
US9070004B2 (en) * 2012-05-03 2015-06-30 General Electric Company Automatic segmentation and characterization of cellular motion
CN103218828B (en) * 2013-03-22 2015-09-30 常熟理工学院 A kind of many cells interaction tracker
US9173909B2 (en) * 2013-05-23 2015-11-03 Drvision Technologies Llc Image guided protocol for cell generation
US20150269314A1 (en) 2014-03-20 2015-09-24 Rudjer Boskovic Institute Method and apparatus for unsupervised segmentation of microscopic color image of unstained specimen and digital staining of segmented histological structures
EP3286337A4 (en) * 2015-04-23 2018-12-12 Cedars-Sinai Medical Center Automated delineation of nuclei for three dimensional (3-d) high content screening
JP6746945B2 (en) 2015-06-30 2020-08-26 ソニー株式会社 Information processing apparatus, information processing system, and information processing method
WO2017002300A1 (en) 2015-06-30 2017-01-05 Sony Corporation Information processing apparatus, information processing system, and information processing method
US10510150B2 (en) * 2017-06-20 2019-12-17 International Business Machines Corporation Searching trees: live time-lapse cell-cycle progression modeling and analysis
CA3071685C (en) 2017-08-09 2023-11-21 Allen Institute Systems, devices, and methods for image processing to generate an image having predictive tagging
US10423819B2 (en) * 2017-10-31 2019-09-24 Chung Yuan Christian University Method and apparatus for image processing and visualization for analyzing cell kinematics in cell culture
EP3734345B1 (en) * 2019-04-30 2023-08-02 Leica Microsystems CMS GmbH Image processing device, system, and method for improving signal-to-noise of microscopy images
US11947270B2 (en) * 2020-02-26 2024-04-02 Fei Company Metrology of semiconductor devices in electron micrographs using fast marching level sets

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2903855A1 (en) * 1979-02-01 1980-08-14 Bloss Werner H Prof Dr Ing METHOD FOR AUTOMATICALLY MARKING CELLS AND DETERMINING THE CHARACTERISTICS OF CELLS FROM CYTOLOGICAL MEASUREMENT DEVICES
DE3836716A1 (en) 1988-10-28 1990-05-03 Zeiss Carl Fa METHOD FOR EVALUATING CELL IMAGES
US5162990A (en) * 1990-06-15 1992-11-10 The United States Of America As Represented By The United States Navy System and method for quantifying macrophage phagocytosis by computer image analysis
US5548661A (en) * 1991-07-12 1996-08-20 Price; Jeffrey H. Operator independent image cytometer
US5361763A (en) 1993-03-02 1994-11-08 Wisconsin Alumni Research Foundation Method for segmenting features in an image
WO1997004418A1 (en) * 1995-07-19 1997-02-06 Morphometrix Technologies Inc. Multi-spectral segmentation for image analysis
AUPP278698A0 (en) * 1998-04-03 1998-04-30 University Of Queensland, The Method of cell nuclei segmentation
US7151847B2 (en) * 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
US7546210B2 (en) * 2000-06-08 2009-06-09 The Regents Of The University Of California Visual-servoing optical microscopy
AUPQ849200A0 (en) * 2000-06-30 2000-07-27 Cea Technologies Inc. Unsupervised scene segmentation
US20030134269A1 (en) * 2000-11-22 2003-07-17 Hiroyuki Hirai Cell proliferation ability evaluation method and apparatus
US6956961B2 (en) * 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
WO2002080110A1 (en) * 2001-03-29 2002-10-10 Koninklijke Philips Electronics N.V. Image processing method for fitness estimation of a 3d mesh model mapped onto a 3d surface of an object
US20040071328A1 (en) * 2001-09-07 2004-04-15 Vaisberg Eugeni A. Classifying cells based on information contained in cell images
JP4749637B2 (en) * 2001-09-28 2011-08-17 オリンパス株式会社 Image analysis method, apparatus, and recording medium
US7756305B2 (en) * 2002-01-23 2010-07-13 The Regents Of The University Of California Fast 3D cytometry for information in tissue engineering
DE10217858C1 (en) * 2002-04-22 2003-10-02 Fraunhofer Ges Forschung Separation of groups of cells in an image, useful e.g. for analysis of cancer-screening smears, based on assignment of overlap areas to individual cells
US7274809B2 (en) 2002-08-29 2007-09-25 Perceptronix Medical, Inc. And British Columbia Cancer Agency Computerized methods and systems related to the detection of malignancy-associated changes (MAC) to detect cancer
WO2004025556A2 (en) * 2002-09-12 2004-03-25 Baylor College Of Medecine System and method for image segmentation
EP1616286A1 (en) * 2003-04-02 2006-01-18 Amersham Biosciences UK Limited Method of, and computer software for, classification of cells into subpopulations
GB0307684D0 (en) 2003-04-02 2003-05-07 Amersham Biosciences Uk Ltd Determining cell cycle phase data
WO2004099773A1 (en) * 2003-04-30 2004-11-18 Pfizer Products Inc. Automated in vitro cellular imaging assays for micronuclei and other target objects
US20060247862A1 (en) * 2003-09-08 2006-11-02 Arini Nicholas S Determining cell cycle phase data
JP3955953B2 (en) * 2003-09-12 2007-08-08 国立大学法人広島大学 Image division processing apparatus, image division processing method, and image division processing integrated circuit
US7580556B2 (en) * 2004-01-26 2009-08-25 Drvision Technologies Llc Image region partitioning using pre-labeled regions
GB2412803A (en) 2004-03-30 2005-10-05 Amersham Biosciences Niagara I Image segmentation using local and remote pixel value comparison
US20050273271A1 (en) * 2004-04-05 2005-12-08 Aibing Rao Method of characterizing cell shape
US7711174B2 (en) * 2004-05-13 2010-05-04 The Charles Stark Draper Laboratory, Inc. Methods and systems for imaging cells
EP1615170A1 (en) 2004-07-10 2006-01-11 Evotec Technologies GmbH Image segmentation algorithms for applications in cellular biology
US20060127881A1 (en) 2004-10-25 2006-06-15 Brigham And Women's Hospital Automated segmentation, classification, and tracking of cell nuclei in time-lapse microscopy

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ACTON S T ET AL: "Data Acceptance for Automated Leukocyte Tracking Through Segmentation of Spatiotemporal Images", IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, IEEE SERVICE CENTER, PISCATAWAY, NJ, US, vol. 52, no. 10, October 2005 (2005-10-01), pages 1702 - 1712, XP011139405, ISSN: 0018-9294 *
PADFIELD D R ET AL: "Spatio-Temporal Cell Cycle Analysis Using 3D Level Set Segmentation of Unstained Nuclei in Line Scan Confocal Fluorescence Images", BIOMEDICAL IMAGING: MACRO TO NANO, 2006. 3RD IEEE INTERNATIONAL SYMPOSIUM ON APRIL 6, 2006, PISCATAWAY, NJ, USA,IEEE, 6 April 2006 (2006-04-06), pages 1036 - 1039, XP010912967, ISBN: 0-7803-9576-X *

Also Published As

Publication number Publication date
EP1946270B1 (en) 2011-11-02
JP2009515533A (en) 2009-04-16
PL1946270T3 (en) 2012-05-31
WO2007097816A2 (en) 2007-08-30
CA2629349C (en) 2014-08-26
ES2373689T3 (en) 2012-02-07
DK1946270T3 (en) 2011-12-12
ATE532154T1 (en) 2011-11-15
AU2006338566A1 (en) 2007-08-30
JP5069245B2 (en) 2012-11-07
CA2629349A1 (en) 2007-08-30
EP1946270A2 (en) 2008-07-23
US20070109874A1 (en) 2007-05-17
AU2006338566B2 (en) 2011-12-15
US7817841B2 (en) 2010-10-19

Similar Documents

Publication Publication Date Title
WO2007097816A3 (en) Time-lapse cell cycle analysis of unstained nuclei
WO2020047010A8 (en) Increasing spatial array resolution
TW200709799A (en) Automated image analysis
IL176462A0 (en) Automated asymmetric threat detection using backward tracking and behavioral analysis
GB0620607D0 (en) Scene analysis
EP1844426A4 (en) Blood analysis using a flow imaging cytometer
AU303917S (en) Analyte test meter
WO2008063783A3 (en) Biomarkers related to metabolic age and methods using the same
EP1702284A4 (en) Contamination estimation using fluid analysis models
GB201006663D0 (en) Microphone array processor based on spatial analysis
IL191064A0 (en) Real time nucleic acid detection in vivo using protein complementation
GB0509833D0 (en) Cell analysis
WO2007060127A3 (en) Device and method for automatically determining the individual three-dimensional shape of particles
WO2009082523A3 (en) High-resolution 3d imaging of single semiconductor nanocrystals
WO2005124356A3 (en) Use of protein cbp2 as a marker for colorectal cancer
CA2511319A1 (en) Rack system
WO2010017524A3 (en) System, method and computer accessible medium for providing real-time diffusional kurtosis imaging
EP1639339A4 (en) Fluid sample analysis using class weights
WO2008131261A3 (en) Peptide biomarkers of cardiovascular disease
WO2007056288A3 (en) Methods of evaluating baff
SG126118A1 (en) Fluorescence measurement analytical kit
AU157704S (en) Analyte test meter
USD693019S1 (en) Laboratory apparatus
EP1855228A4 (en) Information leak analysis system
GB0512840D0 (en) Optical signal analysis system

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2006338566

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2006850118

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2629349

Country of ref document: CA

Ref document number: 2008540324

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2006338566

Country of ref document: AU

Date of ref document: 20061108

Kind code of ref document: A