WO2007106579A3 - Integrated nucleic acid assays - Google Patents

Integrated nucleic acid assays Download PDF

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Publication number
WO2007106579A3
WO2007106579A3 PCT/US2007/006584 US2007006584W WO2007106579A3 WO 2007106579 A3 WO2007106579 A3 WO 2007106579A3 US 2007006584 W US2007006584 W US 2007006584W WO 2007106579 A3 WO2007106579 A3 WO 2007106579A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid assays
disclosed
integrated nucleic
microfluidic cartridges
Prior art date
Application number
PCT/US2007/006584
Other languages
French (fr)
Other versions
WO2007106579A2 (en
Inventor
C Frederick Battrell
John Gerdes
John R Williford
Denise Maxine Hoekstra
Wayne L Breidford
Stephen Mordue
John Clemmens
Melud Nabavi
Mark Kokoris
Original Assignee
Micronics Inc
C Frederick Battrell
John Gerdes
John R Williford
Denise Maxine Hoekstra
Wayne L Breidford
Stephen Mordue
John Clemmens
Melud Nabavi
Mark Kokoris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micronics Inc, C Frederick Battrell, John Gerdes, John R Williford, Denise Maxine Hoekstra, Wayne L Breidford, Stephen Mordue, John Clemmens, Melud Nabavi, Mark Kokoris filed Critical Micronics Inc
Priority to EP07753229A priority Critical patent/EP2007905B1/en
Priority to AU2007225038A priority patent/AU2007225038B2/en
Priority to JP2009500496A priority patent/JP5254949B2/en
Priority to ES07753229T priority patent/ES2393758T3/en
Publication of WO2007106579A2 publication Critical patent/WO2007106579A2/en
Publication of WO2007106579A3 publication Critical patent/WO2007106579A3/en
Priority to US12/207,627 priority patent/US8222023B2/en
Priority to HK09105845.7A priority patent/HK1126824A1/en
Priority to US13/492,612 priority patent/US8772017B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/082—Handling hazardous material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.
PCT/US2007/006584 2006-03-15 2007-03-15 Integrated nucleic acid assays WO2007106579A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP07753229A EP2007905B1 (en) 2006-03-15 2007-03-15 Integrated nucleic acid assays
AU2007225038A AU2007225038B2 (en) 2006-03-15 2007-03-15 Integrated nucleic acid assays
JP2009500496A JP5254949B2 (en) 2006-03-15 2007-03-15 Integrated nucleic acid assay
ES07753229T ES2393758T3 (en) 2006-03-15 2007-03-15 Integrated nucleic acid assays
US12/207,627 US8222023B2 (en) 2006-03-15 2008-09-10 Integrated nucleic acid assays
HK09105845.7A HK1126824A1 (en) 2006-03-15 2009-06-30 Integrated nucleic acid assays
US13/492,612 US8772017B2 (en) 2006-03-15 2012-06-08 Integrated nucleic acid assays

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US78264906P 2006-03-15 2006-03-15
US60/782,649 2006-03-15
US84481106P 2006-09-14 2006-09-14
US60/844,811 2006-09-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/207,627 Continuation US8222023B2 (en) 2006-03-15 2008-09-10 Integrated nucleic acid assays

Publications (2)

Publication Number Publication Date
WO2007106579A2 WO2007106579A2 (en) 2007-09-20
WO2007106579A3 true WO2007106579A3 (en) 2008-02-28

Family

ID=38445613

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2007/006584 WO2007106579A2 (en) 2006-03-15 2007-03-15 Integrated nucleic acid assays
PCT/US2007/006585 WO2007106580A2 (en) 2006-03-15 2007-03-15 Rapid magnetic flow assays

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2007/006585 WO2007106580A2 (en) 2006-03-15 2007-03-15 Rapid magnetic flow assays

Country Status (7)

Country Link
US (3) US20090148847A1 (en)
EP (1) EP2007905B1 (en)
JP (1) JP5254949B2 (en)
AU (1) AU2007225038B2 (en)
ES (1) ES2393758T3 (en)
HK (1) HK1126824A1 (en)
WO (2) WO2007106579A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use

Families Citing this family (307)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
WO2004061085A2 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
CN102759466A (en) 2004-09-15 2012-10-31 英特基因有限公司 Microfluidic devices
US9637715B2 (en) 2005-07-07 2017-05-02 Emd Millipore Corporation Cell culture and invasion assay method and system
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
US9388374B2 (en) 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US9354156B2 (en) 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
WO2007027843A2 (en) * 2005-08-31 2007-03-08 T2 Biosystems, Inc. Nmr device for detection of analytes involving magnetic particles
EP1979079A4 (en) 2006-02-03 2012-11-28 Integenx Inc Microfluidic devices
EP2007905B1 (en) * 2006-03-15 2012-08-22 Micronics, Inc. Integrated nucleic acid assays
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
CA2646309C (en) 2006-04-21 2016-07-26 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
WO2008147382A1 (en) * 2006-09-27 2008-12-04 Micronics, Inc. Integrated microfluidic assay devices and methods
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008115626A2 (en) 2007-02-05 2008-09-25 Microchip Biotechnologies, Inc. Microfluidic and nanofluidic devices, systems, and applications
CA2685382C (en) 2007-05-01 2023-02-21 The Regents Of The University Of California Methods for diagnosing ischemia
DE102007029772B4 (en) * 2007-06-22 2011-12-08 Aj Innuscreen Gmbh Method and rapid test for the detection of specific nucleic acid sequences
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
JP5852781B2 (en) * 2007-07-31 2016-02-03 マイクロニクス， インコーポレイテッド Hygienic swab collection system, microfluidic assay device and method for diagnostic assays
CN101903104B (en) * 2007-10-12 2014-06-25 瑞昂尼克公司 Integrated microfluidic device and methods
US8143896B2 (en) * 2007-10-23 2012-03-27 Abqmr, Inc. Microcoil magnetic resonance detectors
JP5009125B2 (en) * 2007-10-26 2012-08-22 日立マクセル株式会社 Microdevice using functional particles and processing method using the same
EP2072133A1 (en) * 2007-12-20 2009-06-24 Koninklijke Philips Electronics N.V. Multi-compartment device with magnetic particles
EP2245453B1 (en) 2008-01-03 2016-10-05 EMD Millipore Corporation Microfluidic cell culture array system for automated assays and methods of operation
WO2009108260A2 (en) * 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
KR100960066B1 (en) * 2008-05-14 2010-05-31 삼성전자주식회사 Microfluidic device containing lyophilized reagent therein and analysing method using the same
JP2010000409A (en) * 2008-06-18 2010-01-07 Hitachi Maxell Ltd Surface-roughened high-density functional particle, method for producing the same and method for treating target substance by using the same
EP2138587A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Amplification of nucleic acids using temperature zones
GB2463750A (en) * 2008-07-15 2010-03-31 L3 Technology Ltd Assay device comprising surfaces of different surface energies
US8753868B2 (en) * 2008-08-04 2014-06-17 General Electric Company Method and system for selective isolation of target biological molecules in a general purpose system
WO2010025302A2 (en) * 2008-08-27 2010-03-04 Life Technologies Corporation Apparatus for and method of processing biological samples
US11951474B2 (en) 2008-10-22 2024-04-09 Life Technologies Corporation Fluidics systems for sequential delivery of reagents
US8546128B2 (en) 2008-10-22 2013-10-01 Life Technologies Corporation Fluidics system for sequential delivery of reagents
US8435465B2 (en) * 2008-11-03 2013-05-07 Cfd Research Corporation Microfluidic biological extraction chip
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
EP2198965A1 (en) * 2008-12-19 2010-06-23 Koninklijke Philips Electronics N.V. Integrated device for automated simultaneous detection of multiple specific targets using nucleic acid amplification
EP2208531A1 (en) * 2008-12-30 2010-07-21 Atonomics A/S Distribution of particles in capillary channel by application of magnetic field
WO2010077322A1 (en) * 2008-12-31 2010-07-08 Microchip Biotechnologies, Inc. Instrument with microfluidic chip
AU2015215950B2 (en) * 2009-02-03 2017-06-29 Ande Corporation Nucleic Acid Purification
CA2751455C (en) * 2009-02-03 2019-03-12 Netbio, Inc. Nucleic acid purification
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
EP2412020B1 (en) 2009-03-24 2020-09-30 University Of Chicago Slip chip device and methods
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
WO2010120786A1 (en) * 2009-04-13 2010-10-21 Micronics, Inc. Microfluidic clinical analyzer
CN102459565A (en) * 2009-06-02 2012-05-16 尹特根埃克斯有限公司 Fluidic devices with diaphragm valves
EP2437887B1 (en) 2009-06-04 2016-05-11 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
KR20120031218A (en) 2009-06-05 2012-03-30 인터젠엑스 인크. Universal sample preparation system and use in an integrated analysis system
AU2010258716B2 (en) * 2009-06-12 2015-11-12 Perkinelmer Health Sciences, Inc. Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices
WO2010144682A1 (en) 2009-06-12 2010-12-16 Micronics, Inc. Rehydratable matrices for dry storage of taq polymerase in a microfluidic device
US8012770B2 (en) 2009-07-31 2011-09-06 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
EP2309266A1 (en) 2009-09-21 2011-04-13 F. Hoffmann-La Roche AG Method for carrying out reactions in an analytical device
ES2461992T3 (en) 2009-10-09 2014-05-22 Invisible Sentinel, Inc. Device for the detection of antigens and their uses
DE102009045685A1 (en) * 2009-10-14 2011-04-21 Robert Bosch Gmbh Microfluidic chip
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US10022696B2 (en) 2009-11-23 2018-07-17 Cyvek, Inc. Microfluidic assay systems employing micro-particles and methods of manufacture
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US9353342B2 (en) 2010-01-21 2016-05-31 Emd Millipore Corporation Cell culture and gradient migration assay methods and devices
JP5791634B2 (en) * 2010-01-29 2015-10-07 マイクロニクス， インコーポレイテッド Sample-response microfluidic cartridge
US8720036B2 (en) 2010-03-09 2014-05-13 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
KR101519379B1 (en) * 2010-04-29 2015-05-12 삼성전자 주식회사 Centrifugal Micro-fluidic Device and Method for immunoassay
CN105803534A (en) * 2010-05-06 2016-07-27 艾比斯生物科学公司 Integrated sample preparation system and stable enzyme mixture
SG10201503540QA (en) 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
US10132303B2 (en) 2010-05-21 2018-11-20 Hewlett-Packard Development Company, L.P. Generating fluid flow in a fluidic network
US9090084B2 (en) 2010-05-21 2015-07-28 Hewlett-Packard Development Company, L.P. Fluid ejection device including recirculation system
US9395050B2 (en) 2010-05-21 2016-07-19 Hewlett-Packard Development Company, L.P. Microfluidic systems and networks
US9963739B2 (en) * 2010-05-21 2018-05-08 Hewlett-Packard Development Company, L.P. Polymerase chain reaction systems
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US20110312712A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Genetic analysis loc for pcr amplification of nucleic acids from whole blood
US20130088221A1 (en) 2010-06-22 2013-04-11 Koninklijke Philips Electronics N.V. Detection of magnetic particles and their clustering
US20120015904A1 (en) 2010-07-14 2012-01-19 Regents Of The University Of California Biomarkers for diagnosis of transient ischemic attacks
US11402375B2 (en) 2010-08-05 2022-08-02 Abbott Point Of Care Inc. Magnetic immunosensor with trench configuration and method of use
CN103118785B (en) * 2010-08-05 2015-11-25 雅培医护站股份有限公司 The method of immunity using susceptible magnetic microballon to catch and device
US9329175B2 (en) 2010-08-05 2016-05-03 Abbott Point Of Care Inc. Oscillating immunoassay method and device
EP2601526B1 (en) * 2010-08-05 2016-12-21 Abbott Point Of Care, Inc. Magnetic immunosensor and method of use
EP2606154B1 (en) 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
EP2630517B1 (en) 2010-10-22 2016-03-16 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
KR101776215B1 (en) * 2010-10-29 2017-09-08 삼성전자 주식회사 Micro-device for disrupting cells and method of disrupting cells using the same
EP2668501B1 (en) 2011-01-27 2019-06-12 Invisible Sentinel, Inc. Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof
US8603835B2 (en) * 2011-02-10 2013-12-10 Chembio Diagnostic Systems, Inc. Reduced step dual path immunoassay device and method
WO2012121978A2 (en) 2011-03-04 2012-09-13 The Regents Of The University Of California Biomarkers for the diagnosis of lacunar stroke
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
US9469871B2 (en) * 2011-04-14 2016-10-18 Corporos Inc. Methods and apparatus for point-of-care nucleic acid amplification and detection
BR112013026451B1 (en) 2011-04-15 2021-02-09 Becton, Dickinson And Company system and method to perform molecular diagnostic tests on several samples in parallel and simultaneously amplification in real time in plurality of amplification reaction chambers
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
CA2882675C (en) * 2011-10-09 2020-02-25 Bonnie L. GRAY Microfluidic reconfigurable device for multi-plexed sample analysis
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
EP2773892B1 (en) * 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
KR101481054B1 (en) * 2011-11-15 2015-01-14 한국기계연구원 A device for automatically analyzing nucleic acid
US10029041B2 (en) 2011-11-30 2018-07-24 Pdl Biopharma, Inc. Filtration module
SG11201402558QA (en) 2011-12-03 2014-06-27 Emd Millipore Corp Micro-incubation systems for microfluidic cell culture and methods
MX353212B (en) * 2011-12-07 2018-01-08 Cytovera Inc Method and device for sample processing.
US20130161193A1 (en) * 2011-12-21 2013-06-27 Sharp Kabushiki Kaisha Microfluidic system with metered fluid loading system for microfluidic device
WO2013103781A1 (en) * 2012-01-07 2013-07-11 The Regents Of The University Of California Biomarkers for diagnosing ischemia
WO2013113072A1 (en) 2012-02-03 2013-08-08 Axxin Pty Ltd Nucleic acid amplification and detection apparatus and method
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
AU2013230917C1 (en) 2012-03-09 2021-09-16 Invisible Sentinel, Inc. Methods and compositions for detecting multiple analytes with a single signal
US9797006B2 (en) * 2012-04-10 2017-10-24 Keck Graduate Institute Of Applied Life Sciences System and cartridge for efficient nucleic acid testing
US9354159B2 (en) 2012-05-02 2016-05-31 Nanoscopia (Cayman), Inc. Opto-fluidic system with coated fluid channels
WO2013166856A1 (en) * 2012-05-07 2013-11-14 Capitalbio Corporation Microvalve integrated in a microfluidic device and method of use
CA2872527A1 (en) * 2012-05-08 2013-11-14 Northwestern University Cartridge for use in an automated system for isolating an analyte from a sample, and methods of use
US9625465B2 (en) 2012-05-15 2017-04-18 Defined Diagnostics, Llc Clinical diagnostic systems
US9081001B2 (en) * 2012-05-15 2015-07-14 Wellstat Diagnostics, Llc Diagnostic systems and instruments
US9213043B2 (en) 2012-05-15 2015-12-15 Wellstat Diagnostics, Llc Clinical diagnostic system including instrument and cartridge
EP2856178B1 (en) 2012-05-31 2020-07-15 The University of North Carolina at Chapel Hill Dissolution guided wetting of structured surfaces
US20130331298A1 (en) * 2012-06-06 2013-12-12 Great Basin Scientific Analyzer and disposable cartridge for molecular in vitro diagnostics
EP2684609A1 (en) * 2012-07-09 2014-01-15 Biocartis SA Heater for a disposable dignostics cartridge
US20140200167A1 (en) 2012-08-01 2014-07-17 Nanomdx, Inc. Functionally integrated device for multiplex genetic identification
WO2014031786A1 (en) 2012-08-23 2014-02-27 Northwestern University Device with controlled fluid dynamics, for isolation of an analyte from a sample
US9957553B2 (en) 2012-10-24 2018-05-01 Genmark Diagnostics, Inc. Integrated multiplex target analysis
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
US9624487B2 (en) 2012-11-02 2017-04-18 California Institute Of Technology Methods and apparatuses for nucleic acid purification
US9549666B2 (en) 2012-11-10 2017-01-24 Curvo Medical, Inc. Coaxial micro-endoscope
US9233225B2 (en) 2012-11-10 2016-01-12 Curvo Medical, Inc. Coaxial bi-directional catheter
US9289761B2 (en) * 2012-11-16 2016-03-22 Honeywell International Inc. Card waste storage mechanism
WO2014083799A1 (en) * 2012-11-27 2014-06-05 日本電気株式会社 Fluidic chip and waste liquid processing method for same
CN103849548A (en) 2012-12-03 2014-06-11 三星电子株式会社 Reagent container for amplifying nucleic acid, method of preparing the reagent container, method of storing the reagent, and microfluidic system for nucleic acid analysis
US9394637B2 (en) 2012-12-13 2016-07-19 Jacob Holm & Sons Ag Method for production of a hydroentangled airlaid web and products obtained therefrom
WO2014097287A1 (en) * 2012-12-17 2014-06-26 Leukodx, Ltd. Systems and methods for detecting a biological condition
US9759722B2 (en) 2012-12-17 2017-09-12 Leukodx Ltd. Systems and methods for determining a chemical state
US10610861B2 (en) 2012-12-17 2020-04-07 Accellix Ltd. Systems, compositions and methods for detecting a biological condition
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
CN104919035B (en) 2012-12-21 2017-08-11 精密公司 Portable fluorescence detecting system and micro- determination box
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
EP2934751B1 (en) 2012-12-21 2019-05-29 Micronics, Inc. Low elasticity films for microfluidic use
KR101984699B1 (en) * 2013-01-24 2019-05-31 삼성전자주식회사 Micro-fluidic system for analysis of nucleic acid
JP6202713B2 (en) * 2013-02-22 2017-09-27 株式会社日立ハイテクノロジーズ Biochemical cartridge and biochemical feed system
PE20151873A1 (en) * 2013-03-14 2016-01-06 Kasing Cheng TEST STRIP HOUSING SYSTEM
WO2014144782A2 (en) 2013-03-15 2014-09-18 Ancera, Inc. Systems and methods for active particle separation
EP2969218A2 (en) * 2013-03-15 2016-01-20 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
WO2014145765A1 (en) * 2013-03-15 2014-09-18 Ancera, Inc. Systems and methods for bead-based assays in ferrofluids
CN105228748B (en) 2013-03-15 2017-10-10 金马克诊断股份有限公司 System, method and apparatus for manipulating deformable fluid container
EP2972336A4 (en) * 2013-03-15 2016-11-16 Nvs Technologies Inc Analytical instrument systems
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
RU2530170C1 (en) * 2013-03-27 2014-10-10 Михаил Аркадьевич Шурдов Method of detecting stem cancer cells
US10254279B2 (en) * 2013-03-29 2019-04-09 Nima Labs, Inc. System and method for detection of target substances
EP2979092B1 (en) 2013-03-29 2018-05-30 Nima Labs, Inc. A portable device for detection of harmful substances
US10466236B2 (en) 2013-03-29 2019-11-05 Nima Labs, Inc. System and method for detecting target substances
US20150056687A1 (en) * 2013-04-26 2015-02-26 Express Diagnostics Int'l., Inc. Lateral flow devices and methods of manufacture and use
CA2911303C (en) 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
WO2014182847A1 (en) * 2013-05-07 2014-11-13 Micronics, Inc. Device for preparation and analysis of nucleic acids
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
TWI529402B (en) * 2013-07-26 2016-04-11 財團法人工業技術研究院 Magnetic beads manipulating device and method for manipulating magnetic beads
GB2516669B (en) * 2013-07-29 2015-09-09 Atlas Genetics Ltd A method for processing a liquid sample in a fluidic cartridge
GB2516667A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd An improved cartridge, cartridge reader and method for preventing reuse
GB2516666B (en) * 2013-07-29 2015-09-09 Atlas Genetics Ltd Fluidic cartridge for nucleic acid amplification and detection
GB2516672B (en) 2013-07-29 2015-05-20 Atlas Genetics Ltd A system and method for expelling liquid from a fluidic cartridge
GB2516675A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd A valve which depressurises, and a valve system
DE102013219502A1 (en) * 2013-09-27 2015-04-02 Robert Bosch Gmbh Analysis unit for carrying out a polymerase chain reaction, method for operating such an analysis unit and method for producing such an analysis unit
WO2015061480A1 (en) * 2013-10-22 2015-04-30 Corporos Inc. Methods and apparatus for point-of-care nucleic acid amplification and detection
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
CN103602583A (en) * 2013-11-07 2014-02-26 苏州汶颢芯片科技有限公司 Integrated multifunctional microfluidic chip
CN110560187B (en) 2013-11-18 2022-01-11 尹特根埃克斯有限公司 Cartridge and instrument for sample analysis
EP3080294B1 (en) 2013-12-12 2018-06-13 Altratech Limited A capacitive sensor and method of use
EP2883961B8 (en) 2013-12-12 2017-09-27 Altratech Limited A nucleic acid analysis method and apparatus
WO2015086652A1 (en) 2013-12-12 2015-06-18 Altra Tech Limited A sample preparation method and apparatus
CN105980058A (en) 2014-01-07 2016-09-28 达克雷诊断器材有限公司 Fluid delivery devices, systems, and methods
WO2015112914A1 (en) 2014-01-24 2015-07-30 Life Technologies Corporation Purification chemistries and formats for sanger dna sequencing reactions on a micro-fluidics device
EP2905079A1 (en) * 2014-02-10 2015-08-12 Robert Bosch Gmbh Device for storing a fluid in a microfluidic system, method for operating and method for producing such a device
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
US10300484B2 (en) 2014-03-26 2019-05-28 The George Washington University Handheld fluid handling systems and methods
EP3126486B1 (en) 2014-04-02 2019-07-03 Chembio Diagnostic Systems, Inc. Immunoassay utilizing trapping conjugate
GB201407334D0 (en) 2014-04-25 2014-06-11 Dna Electronics Ltd Integrated nucleic acid test system, instrument and method
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
US9890424B2 (en) * 2014-06-17 2018-02-13 Life Technologies Corporation Sequencing device
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
US10472620B2 (en) * 2014-07-01 2019-11-12 General Electric Company Method, substrate and device for separating nucleic acids
US9593368B2 (en) 2014-07-01 2017-03-14 General Electric Company Methods for amplifying nucleic acids on substrates
US11098347B2 (en) 2014-07-08 2021-08-24 National Institute Of Advanced Industrial Science And Technology Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification
CN106796212A (en) 2014-08-12 2017-05-31 新生代吉恩公司 System and method for monitoring health based on the body fluid collected
EP3210020A1 (en) * 2014-10-20 2017-08-30 Christopher Gordon Atwood Analyzing a sample with a construct comprising a fluorescent moiety and a magnetic moiety
EP3552690A1 (en) 2014-10-22 2019-10-16 IntegenX Inc. Systems and methods for sample preparation, processing and analysis
WO2016065298A1 (en) * 2014-10-24 2016-04-28 Ibis Biosciences, Inc. Systems, compositions and methods for size selective nucleic acid purification
US20160116466A1 (en) 2014-10-27 2016-04-28 Chembio Diagnostic Systems, Inc. Rapid Screening Assay for Qualitative Detection of Multiple Febrile Illnesses
US20160123975A1 (en) * 2014-11-03 2016-05-05 Daktari Diagnostics, Inc. Mesh Microfluidic Mixing Chamber
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
EP3218698B1 (en) 2014-11-14 2020-05-13 Axxin Pty Ltd Biological sample collection and storage assembly
WO2016085841A1 (en) * 2014-11-24 2016-06-02 Nanostring Technologies, Inc. Methods and apparatuses for gene purification and imaging
US10416065B2 (en) * 2014-12-16 2019-09-17 Celldynamics I.S.R.L. Device for real time analysis of particles suspended in a fluid and method for the analysis of said particles
MX2017008618A (en) 2014-12-31 2018-03-23 Click Diagnostics Inc Devices and methods for molecular diagnostic testing.
WO2016117725A1 (en) * 2015-01-23 2016-07-28 Infopia Co., Ltd. Molecular diagnostic cartridge
WO2016117726A1 (en) * 2015-01-23 2016-07-28 Infopia Co., Ltd. Cartridge
GB201501705D0 (en) 2015-02-02 2015-03-18 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB2531615B (en) * 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
WO2016128570A1 (en) * 2015-02-13 2016-08-18 Espci Paper device for genetic diagnosis
WO2016210348A2 (en) 2015-06-26 2016-12-29 Ancera, Inc. Background defocusing and clearing in ferrofluid-based capture assays
WO2017003924A1 (en) * 2015-06-29 2017-01-05 Genesis DNA Inc. Method and apparatus for dual solid phase nucleic acid synthesis
AU2016295422B2 (en) * 2015-07-17 2022-01-06 Axxin Pty Ltd Diagnostic test assembly, apparatus, method
TWI591182B (en) 2015-07-17 2017-07-11 台達電子工業股份有限公司 Nucleic acid extracting device
CN109966776B (en) * 2017-12-26 2021-07-13 台达电子工业股份有限公司 Nucleic acid extraction method and extraction cartridge thereof
US11207681B2 (en) 2015-07-17 2021-12-28 Delta Electronics, Inc. Method for extracting nucleic acid and extraction cassette thereof
US10775370B2 (en) * 2015-07-17 2020-09-15 Stat-Diagnostica & Innovation, S.L. Fluidic system for performing assays
US11491482B2 (en) 2015-07-17 2022-11-08 Delta Electronics, Inc. Method for extracting nucleic acid and extraction cassette thereof
WO2017019221A1 (en) * 2015-07-24 2017-02-02 HJ Science & Technology, Inc. Reconfigurable microfluidic systems: homogeneous assays
FR3040141B1 (en) * 2015-08-20 2020-02-14 Commissariat A L'energie Atomique Et Aux Energies Alternatives FLUIDIC CARD COMPRISING AT LEAST ONE FLUIDIC VALVE
WO2017053817A1 (en) 2015-09-25 2017-03-30 The Arizona Board Of Regents On Behalf Of The University Of Arizona Thermally-actuated valve for metering of biological samples
US10301667B2 (en) * 2015-11-18 2019-05-28 University Of Florida Research Foundation, Inc. Devices for detecting target biological molecules from cells and viruses
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
US11371075B2 (en) 2016-01-08 2022-06-28 Advanced Theranostics Inc. Fully integrated hand-held device to detect specific nucleic acid sequences
WO2017127731A1 (en) 2016-01-21 2017-07-27 T2 Biosystems, Inc. Nmr methods and systems for the rapid detection of bacteria
KR102521127B1 (en) * 2016-02-19 2023-04-13 퍼킨엘머 헬스 사이언시즈, 아이엔씨. Microfluidic mixing device and method
BR112018067739A2 (en) * 2016-03-04 2019-01-08 Alere San Diego Inc automated nested recombinase polymerase amplification
WO2017180909A1 (en) 2016-04-13 2017-10-19 Nextgen Jane, Inc. Sample collection and preservation devices, systems and methods
JP6982338B2 (en) * 2016-04-20 2021-12-17 Ｂｌｕｅ Ｉｎｄｕｓｔｒｉｅｓ株式会社 Pretreatment kit for gene analysis, chip for nucleic acid analysis, analysis system, chip for biomaterial analysis
WO2017185067A1 (en) 2016-04-22 2017-10-26 Click Diagnostics, Inc. Printed circuit board heater for an amplification module
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
US9933445B1 (en) 2016-05-16 2018-04-03 Hound Labs, Inc. System and method for target substance identification
WO2017213129A1 (en) * 2016-06-06 2017-12-14 株式会社ニコン Fluid device
WO2018005710A1 (en) 2016-06-29 2018-01-04 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
USD800331S1 (en) 2016-06-29 2017-10-17 Click Diagnostics, Inc. Molecular diagnostic device
USD800913S1 (en) 2016-06-30 2017-10-24 Click Diagnostics, Inc. Detection window for molecular diagnostic device
USD800914S1 (en) 2016-06-30 2017-10-24 Click Diagnostics, Inc. Status indicator for molecular diagnostic device
US10626454B2 (en) * 2016-07-27 2020-04-21 SeqMatic, LLC Compositions and methods for nucleic acid amplification and analysis
GB201615320D0 (en) * 2016-09-09 2016-10-26 Invitron Ltd Point of care platform test
AU2017325833B2 (en) * 2016-09-15 2022-06-16 Abbott Laboratories Devices and methods for sample analysis
WO2018094104A1 (en) * 2016-11-17 2018-05-24 Brisa Biotech Llc Pressure driven fluidics device and analyte detection systems
WO2018183723A1 (en) * 2017-03-29 2018-10-04 Cornell University Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions
EP3607087A4 (en) 2017-04-04 2020-12-30 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
DE102017206155A1 (en) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption of nucleic acids
US20220097063A1 (en) * 2017-05-16 2022-03-31 Autonomous Medical Devices Inc. Apparatus for Automatic Sampling of Biological Species Employing Disk Microfluidics System
US11224874B2 (en) * 2017-12-11 2022-01-18 Autonomous Medical Devices Inc. Apparatus for automatic sampling of biological species employing disk microfluidics system
US11358140B2 (en) * 2017-05-16 2022-06-14 Autonomous Medical Devices Inc. Apparatus for automatic sampling of biological species employing an amplification with a magnetic nanoparticle and propulsion method
EP3684950B1 (en) 2017-09-20 2023-04-26 Altratech Limited Diagnostic device and system
CA3076836A1 (en) 2017-09-27 2019-04-04 Axxin Pty Ltd Diagnostic test system and method
US20200332342A1 (en) * 2017-10-20 2020-10-22 Hewlett-Packard Development Company, L.P. Nucleic acid amplification
WO2019094784A1 (en) * 2017-11-09 2019-05-16 Click Diagnostics, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
GB201718917D0 (en) * 2017-11-16 2018-01-03 Ge Healthcare Bioprocess R&D Ab Method and system for manufacturing of biopharmaceutical products
US10610843B2 (en) 2017-11-28 2020-04-07 Talis Biomedical Corporation Magnetic mixing apparatus
EP3495811A1 (en) * 2017-12-11 2019-06-12 Sensor-Kinesis Corporation Field portable handheld, recirculating surface acoustic wave and method for operating the same
NL2020419B1 (en) 2017-12-11 2019-06-19 Sensor Kinesis Corp Field portable, handheld, recirculating surface acoustic wave and method for operating the same
JP7040934B2 (en) * 2017-12-21 2022-03-23 積水化学工業株式会社 Microchip
KR102056938B1 (en) * 2018-01-26 2019-12-17 (주)메타포어 Membrane structure with matrix structure and biomolecule filter using the same
KR102105558B1 (en) * 2018-03-23 2020-04-28 (주)바이오니아 Analysis Plate For Polymerase Chain Reaction
WO2020018074A1 (en) 2018-07-17 2020-01-23 Hewlett-Packard Development Company, L.P. Droplet ejectors to provide fluids to droplet ejectors
WO2019209374A1 (en) 2018-04-24 2019-10-31 Hewlett-Packard Development Company, L.P. Sequenced droplet ejection to deliver fluids
US11925932B2 (en) 2018-04-24 2024-03-12 Hewlett-Packard Development Company, L.P. Microfluidic devices
CA3098905A1 (en) 2018-04-30 2019-11-07 Protein Fluidics, Inc. Valveless fluidic switching flowchip and uses thereof
US11547993B2 (en) 2018-07-17 2023-01-10 Hewlett-Packard Development Company, L.P. Droplet ejectors with target media
EP3841216A1 (en) * 2018-08-16 2021-06-30 Life Technologies Corporation System and method for preparing a sequencing device
US11314375B2 (en) 2018-10-01 2022-04-26 Precigenome, LLC Multichannel pressure control system with user friendly interface
CN109576345A (en) * 2018-10-17 2019-04-05 西人马（厦门）科技有限公司 A kind of micro-fluidic chip and its detection method for DNA extraction
KR102103084B1 (en) * 2018-11-19 2020-04-21 인제대학교 산학협력단 A Microfluidic Device for Separating Fine Particles in Fluid and Measuring Flow velocity
GB201819417D0 (en) * 2018-11-29 2019-01-16 Quantumdx Group Ltd Vacuum-assisted drying of filters in microfluidic systems
GB201819419D0 (en) * 2018-11-29 2019-01-16 Quantumdx Group Ltd Improved microfluidic devices, systems and methods
US20220010359A1 (en) * 2018-12-14 2022-01-13 Hewlett-Packard Development Company, L.P. Decision tree polymerase chain reaction
US11567068B2 (en) * 2019-03-28 2023-01-31 Autonomous Medical Devices Inc. Detection of cardiac troponin or biological markers via shear horizontal surface acoustic wave biosensor using a wet-dry bioanalytical technique
EP3951402A4 (en) * 2019-04-02 2023-01-18 H.U. Group Research Institute G.K. Flow channel device and inspection system
DE102019117413A1 (en) * 2019-06-27 2020-12-31 Testo SE & Co. KGaA Flow test unit, set and use of a flow test unit to carry out a detection reaction
CN110257245A (en) * 2019-07-16 2019-09-20 东莞博识生物科技有限公司 Nucleic acid detection reagent card
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
FR3100463B1 (en) * 2019-09-11 2021-12-17 Commissariat Energie Atomique Microfluidic device and microfluidic card including said device
WO2021113065A1 (en) * 2019-12-04 2021-06-10 Becton, Dickinson And Company Magnetic capture bead mediated molecular barcoding of nucleic acid targets in single particles and compositions for use in the same
CN110964716A (en) * 2019-12-05 2020-04-07 广州万孚生物技术股份有限公司 In-vitro diagnosis and analysis device and reagent card
CN111073810A (en) * 2019-12-20 2020-04-28 深圳市华迈生物医疗科技有限公司 Microfluidic chip, system and method integrating nucleic acid extraction, amplification and detection
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
WO2021164021A1 (en) * 2020-02-21 2021-08-26 Boe Technology Group Co., Ltd. Microfluidic structure, microfluidic system, microfluidic method, and method of fabricating microfluidic structure
JP7290345B2 (en) * 2020-02-25 2023-06-13 Ｂｌｕｅ Ｉｎｄｕｓｔｒｉｅｓ株式会社 Genetic analysis pretreatment kit, nucleic acid analysis chip, analysis system, biomaterial analysis chip
CN111518668B (en) * 2020-05-06 2023-08-22 上海思路迪生物医学科技有限公司 Microfluidic system for exosome extraction and detection
US11933731B1 (en) 2020-05-13 2024-03-19 Hound Labs, Inc. Systems and methods using Surface-Enhanced Raman Spectroscopy for detecting tetrahydrocannabinol
WO2021247539A1 (en) * 2020-06-02 2021-12-09 Florida Atlantic University Board Of Trustees Automated system and methods for disease detection
CN112024000A (en) * 2020-08-03 2020-12-04 靳秀梅 Clinical laboratory is with test tube device of moisturizing
AU2021356770A1 (en) * 2020-10-07 2023-06-01 Accellix Ltd. System for determining a biological condition and cartridge therefor
CN114350496B (en) * 2020-10-19 2023-12-08 成都瀚辰光翼生物工程有限公司 Gene detection apparatus
CN116490239A (en) 2020-11-09 2023-07-25 敏捷设备有限公司 Device for steering a catheter
US20220155188A1 (en) * 2020-11-16 2022-05-19 Koninklijke Fabriek Inventum B.V. Methods to obtain a biological sample representative of a passenger cabin on an aircraft automatically from the collector device
DE102020135053B4 (en) 2020-12-29 2022-12-15 Biflow Systems Gmbh Microfluidic device with residue container and analysis system
US20240117452A1 (en) * 2021-01-06 2024-04-11 Salus Discovery, LLC Systems and methods for isolating a target from a biological sample
EP4026615A1 (en) * 2021-01-11 2022-07-13 Curiosity Diagnostics sp. z o.o Microfluidic circuit, microfluidic chip, kit and method for isolating and purifying an analyte from a biologic sample
US11806711B1 (en) * 2021-01-12 2023-11-07 Hound Labs, Inc. Systems, devices, and methods for fluidic processing of biological or chemical samples using flexible fluidic circuits
DE102021204952A1 (en) * 2021-05-17 2022-11-17 Robert Bosch Gesellschaft mit beschränkter Haftung Method for purifying nucleic acids, in particular in a microfluidic device
WO2023288108A1 (en) * 2021-07-16 2023-01-19 The University Of Chicago Biocompatible surface for quantum sensing and methods thereof
CN114307247B (en) * 2021-12-13 2023-04-25 重庆安全技术职业学院 Permeation type solid phase microextraction micro-fluidic device
GB202118917D0 (en) * 2021-12-23 2022-02-09 Osler Diagnostics Ltd Liquid handling method, system and device
EP4206324A1 (en) * 2022-01-04 2023-07-05 Phynexus, Inc. Purification of macromolecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO2005106024A1 (en) * 2004-04-30 2005-11-10 Siemens Aktiengesellschaft Method and assembly for dna isolation with dry reagents

Family Cites Families (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622871A (en) * 1987-04-27 1997-04-22 Unilever Patent Holdings B.V. Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
US4610678A (en) * 1983-06-24 1986-09-09 Weisman Paul T High-density absorbent structures
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5038852A (en) * 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
CA1303983C (en) * 1987-03-27 1992-06-23 Robert W. Rosenstein Solid phase assay
EP1248112A3 (en) * 1987-04-27 2004-08-25 Inverness Medical Switzerland GmbH Immunochromatographic specific binding assay device
US4855240A (en) * 1987-05-13 1989-08-08 Becton Dickinson And Company Solid phase assay employing capillary flow
US4943522A (en) * 1987-06-01 1990-07-24 Quidel Lateral flow, non-bibulous membrane assay protocols
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Method and kits for the amplification and detection of nucleic acid sequences
US5120643A (en) * 1987-07-13 1992-06-09 Abbott Laboratories Process for immunochromatography with colloidal particles
US4956302A (en) * 1987-09-11 1990-09-11 Abbott Laboratories Lateral flow chromatographic binding assay device
US5275785A (en) * 1987-10-30 1994-01-04 Unilever Patent Holdings B.V. Test device for detecting an analyte in a liquid sample
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
EP0359789B1 (en) 1988-01-21 1993-08-04 Genentech, Inc. Amplification and detection of nucleic acid sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
JP2856804B2 (en) 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション Catalytic hybridization system for nucleic acid sequence detection based on activity as a cofactor in catalysis to cleave labeled complementary nucleic acid probes
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA1339731C (en) 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5075078A (en) * 1989-10-05 1991-12-24 Abbott Laboratories Self-performing immunochromatographic device
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US6787338B2 (en) * 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
EP0566695B1 (en) * 1991-01-11 1999-06-02 Quidel Corporation A one-step lateral flow assay and nonbibulous support used therein
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US6953676B1 (en) * 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5635602A (en) * 1993-08-13 1997-06-03 The Regents Of The University Of California Design and synthesis of bispecific DNA-antibody conjugates
EP0653639B1 (en) * 1993-11-12 2000-03-22 Unilever Plc Analytical devices and methods of use thereof
GB9419267D0 (en) * 1994-09-23 1994-11-09 Unilever Plc Assay devices
DE4435107C1 (en) * 1994-09-30 1996-04-04 Biometra Biomedizinische Analy Miniaturized flow thermal cycler
DE69621507T2 (en) * 1995-03-28 2003-01-09 Japan Science & Tech Corp Method for molecular indexing of genes using restriction enzymes
US5712172A (en) * 1995-05-18 1998-01-27 Wyntek Diagnostics, Inc. One step immunochromatographic device and method of use
DE69619400T2 (en) * 1995-06-16 2002-09-26 Univ Washington Seattle FLAT MICROPRODUCED CROSS-FLOW FILTER FOR LIQUIDS
DE69628016T2 (en) * 1995-06-16 2004-04-01 University Of Washington, Seattle MINIATURIZED DIFFERENTIAL EXTRACTION DEVICE AND METHOD
US5716852A (en) * 1996-03-29 1998-02-10 University Of Washington Microfabricated diffusion-based chemical sensor
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5724404A (en) * 1995-07-03 1998-03-03 Garcia; Max Integrated international telephone circuit monitoring system
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
US5660990A (en) * 1995-08-18 1997-08-26 Immunivest Corporation Surface immobilization of magnetically collected materials
US5726751A (en) * 1995-09-27 1998-03-10 University Of Washington Silicon microchannel optical flow cytometer
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5741647A (en) * 1996-02-16 1998-04-21 Tam; Joseph Wing On Flow through nucleic acid hybridisation uses thereof and a device thereof
US5972721A (en) * 1996-03-14 1999-10-26 The United States Of America As Represented By The Secretary Of The Air Force Immunomagnetic assay system for clinical diagnosis and other purposes
US5747349A (en) * 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
US5948684A (en) * 1997-03-31 1999-09-07 University Of Washington Simultaneous analyte determination and reference balancing in reference T-sensor devices
US6541213B1 (en) * 1996-03-29 2003-04-01 University Of Washington Microscale diffusion immunoassay
US6057167A (en) * 1996-05-31 2000-05-02 Motorola, Inc. Magnetoresistance-based method and apparatus for molecular detection
US5863801A (en) * 1996-06-14 1999-01-26 Sarnoff Corporation Automated nucleic acid isolation
DE69728269T2 (en) * 1996-06-14 2005-03-10 University Of Washington, Seattle ABSORBENT IMPROVED DIFFERENTIAL EXTRACTION PROCESS
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US5798273A (en) * 1996-09-25 1998-08-25 Becton Dickinson And Company Direct read lateral flow assay for small analytes
US5748827A (en) * 1996-10-23 1998-05-05 University Of Washington Two-stage kinematic mount
US5971355A (en) * 1996-11-27 1999-10-26 Xerox Corporation Microdevice valve structures to fluid control
US5906602A (en) * 1997-03-27 1999-05-25 The Procter & Gamble Company Shaped absorbent cores comprising multiple pieces of absorbent material and method for making same
US5974867A (en) * 1997-06-13 1999-11-02 University Of Washington Method for determining concentration of a laminar sample stream
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US5965410A (en) * 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6007775A (en) 1997-09-26 1999-12-28 University Of Washington Multiple analyte diffusion based chemical sensor
US6953675B2 (en) * 1997-11-06 2005-10-11 Immunomedics, Inc. Landscaped antibodies and antibody fragments for clinical use
US6210882B1 (en) * 1998-01-29 2001-04-03 Mayo Foundation For Medical Education And Reseach Rapid thermocycling for sample analysis
US6018616A (en) * 1998-02-23 2000-01-25 Applied Materials, Inc. Thermal cycling module and process using radiant heat
EP1163052B1 (en) * 1999-02-23 2010-06-02 Caliper Life Sciences, Inc. Manipulation of microparticles in microfluidic systems
US6171850B1 (en) * 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6878540B2 (en) * 1999-06-25 2005-04-12 Cepheid Device for lysing cells, spores, or microorganisms
US6815160B1 (en) * 1999-07-28 2004-11-09 Chiron Corporation Hepatitis C viral antigen immunoassay detection systems
WO2001026813A2 (en) * 1999-10-08 2001-04-19 Micronics, Inc. Microfluidics without electrically of mechanically operated pumps
US20040121364A1 (en) * 2000-02-07 2004-06-24 Mark Chee Multiplex nucleic acid reactions
EP1263533B1 (en) 2000-03-14 2010-03-03 Micronics, Inc. Microfluidic analysis cartridge
CA2408574A1 (en) * 2000-05-24 2001-11-29 Micronics, Inc. Microfluidic concentration gradient loop
US6431212B1 (en) * 2000-05-24 2002-08-13 Jon W. Hayenga Valve for use in microfluidic structures
US7087414B2 (en) * 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US20020015959A1 (en) * 2000-06-23 2002-02-07 Bardell Ronald L. Fluid mixing in microfluidic structures
AU2000274922A1 (en) * 2000-08-08 2002-02-18 Aviva Biosciences Corporation Methods for manipulating moieties in microfluidic systems
EP1311854A2 (en) * 2000-08-23 2003-05-21 Imego AB Microfuidic device and method with trapping of sample in carities having lids that can be opened or closed
DE60140553D1 (en) * 2000-09-14 2009-12-31 Caliper Life Sciences Inc MICROFLUIDIC DEVICES AND METHODS FOR CARRYING OUT TEMPERATURE-MEDIATED REACTIONS
EP1325332B1 (en) * 2000-10-03 2006-12-20 Minerva Biotechnologies Corporation Magnetic in situ dilution
WO2002097398A2 (en) * 2000-10-25 2002-12-05 Exiqon A/S Closed substrate platforms suitable for analysis of biomolecules
EP1350029B1 (en) * 2001-01-08 2014-09-10 President and Fellows of Harvard College Valves and pumps for microfluidic systems and method for making microfluidic systems
US20020160518A1 (en) * 2001-04-03 2002-10-31 Hayenga Jon W. Microfluidic sedimentation
AU2002307152A1 (en) * 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6802342B2 (en) 2001-04-06 2004-10-12 Fluidigm Corporation Microfabricated fluidic circuit elements and applications
US6562209B1 (en) * 2001-04-19 2003-05-13 Northrop Grumman Corporation Automated computer controlled reporter device for conducting imunnoassay and molecular biology procedures
US6418968B1 (en) * 2001-04-20 2002-07-16 Nanostream, Inc. Porous microfluidic valves
US20030032028A1 (en) * 2001-06-12 2003-02-13 Gayle Dace In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads
US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
US7141416B2 (en) * 2001-07-12 2006-11-28 Burstein Technologies, Inc. Multi-purpose optical analysis optical bio-disc for conducting assays and various reporting agents for use therewith
WO2003010563A2 (en) * 2001-07-24 2003-02-06 Burstein Technologies, Inc. Magnetic assisted detection of magnetic beads using optical disc drives
EP1432895B1 (en) * 2001-09-27 2012-11-14 Robert Bosch Gmbh Actuator
GB0124341D0 (en) * 2001-10-10 2001-11-28 Randox Lab Ltd Assay
US6554591B1 (en) * 2001-11-26 2003-04-29 Motorola, Inc. Micropump including ball check valve utilizing ceramic technology and method of fabrication
US7691333B2 (en) * 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
KR20040068968A (en) * 2001-12-21 2004-08-02 코닌클리케 필립스 일렉트로닉스 엔.브이. Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array
US6748975B2 (en) * 2001-12-26 2004-06-15 Micralyne Inc. Microfluidic valve and method of manufacturing same
US7223371B2 (en) * 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
AUPS159702A0 (en) * 2002-04-09 2002-05-16 Tong, Sun Wing Molecular detection and assay by magneto-thermal biochip micro-assay
US6955738B2 (en) * 2002-04-09 2005-10-18 Gyros Ab Microfluidic devices with new inner surfaces
US20040033627A1 (en) * 2002-05-31 2004-02-19 The Regents Of The University Of California Method and apparatus for detecting substances of interest
JP2004037338A (en) * 2002-07-05 2004-02-05 Yokogawa Electric Corp Method for immobilizing biological polymers to substrate using magnetic beads, and biological polymer measuring apparatus employing the same
US20040018611A1 (en) * 2002-07-23 2004-01-29 Ward Michael Dennis Microfluidic devices for high gradient magnetic separation
US6817373B2 (en) * 2002-07-26 2004-11-16 Applera Corporation One-directional microball valve for a microfluidic device
WO2004061085A2 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
US20050221281A1 (en) * 2003-01-08 2005-10-06 Ho Winston Z Self-contained microfluidic biochip and apparatus
EP1587623A2 (en) * 2003-01-14 2005-10-26 Micronics, Inc. Microfluidic devices for fluid manipulation and analysis
US7419638B2 (en) * 2003-01-14 2008-09-02 Micronics, Inc. Microfluidic devices for fluid manipulation and analysis
KR20050118668A (en) * 2003-01-21 2005-12-19 마이크로닉스 인코포레이티드. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
ATE444794T1 (en) * 2003-03-08 2009-10-15 Ecole Polytech MANIPULATION AND TRANSPORT DEVICE FOR MAGNETIC BEADS
EP1633481A1 (en) * 2003-06-06 2006-03-15 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
TWI269873B (en) * 2003-08-29 2007-01-01 Asahi Kasei Corp Biosensor and method of measuring object to be measured
US7329742B2 (en) * 2003-09-04 2008-02-12 The Regents Of The University Of California Aptamers and methods for their in vitro selection and uses thereof
US7575862B2 (en) * 2003-12-09 2009-08-18 Asiagen Corporation Assay systems, kits and methods for detecting microorganisms
WO2005069015A1 (en) * 2004-01-15 2005-07-28 Japan Science And Technology Agency Chemical analysis apparatus and method of chemical analysis
US20050164373A1 (en) * 2004-01-22 2005-07-28 Oldham Mark F. Diffusion-aided loading system for microfluidic devices
EP1733023B1 (en) * 2004-02-24 2013-01-23 Thermal Gradient Thermal cycling device
CN101432698B (en) * 2004-06-07 2012-06-06 伊库姆公司 Sample processing method
US20070264159A1 (en) * 2004-08-20 2007-11-15 Daniel Leonard Vincent Graham Bio-Electronic Device
US7550267B2 (en) * 2004-09-23 2009-06-23 University Of Washington Microscale diffusion immunoassay utilizing multivalent reactants
US20060094028A1 (en) 2004-11-04 2006-05-04 Welch Allyn, Inc. Rapid diagnostic assay
WO2006076567A2 (en) * 2005-01-13 2006-07-20 Micronics, Inc. Microfluidic rare cell detection device
US20070042427A1 (en) * 2005-05-03 2007-02-22 Micronics, Inc. Microfluidic laminar flow detection strip
US20060264779A1 (en) * 2005-05-09 2006-11-23 Kemp Timothy M Fluidic medical devices and uses thereof
AU2006251637B2 (en) 2005-05-20 2012-06-14 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
CA2610875A1 (en) * 2005-06-06 2006-12-14 Decision Biomarkers, Inc. Assays based on liquid flow over arrays
US7763453B2 (en) * 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
US20090061450A1 (en) * 2006-03-14 2009-03-05 Micronics, Inc. System and method for diagnosis of infectious diseases
EP2007905B1 (en) * 2006-03-15 2012-08-22 Micronics, Inc. Integrated nucleic acid assays
US7718193B2 (en) 2006-03-16 2010-05-18 University Of Washington Temperature- and pH-responsive polymer compositions
AU2007265628B2 (en) * 2006-06-23 2012-12-06 Perkinelmer Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
EP2202328A1 (en) 2008-12-26 2010-06-30 Fundacion Inasmet Process for obtaining protective coatings for high temperature with high roughness and coating obtained

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO2005106024A1 (en) * 2004-04-30 2005-11-10 Siemens Aktiengesellschaft Method and assembly for dna isolation with dry reagents

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use

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