WO2007136386A3 - Droplet-based on-chip sample preparation for mass spectrometry - Google Patents

Droplet-based on-chip sample preparation for mass spectrometry Download PDF

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Publication number
WO2007136386A3
WO2007136386A3 PCT/US2006/021699 US2006021699W WO2007136386A3 WO 2007136386 A3 WO2007136386 A3 WO 2007136386A3 US 2006021699 W US2006021699 W US 2006021699W WO 2007136386 A3 WO2007136386 A3 WO 2007136386A3
Authority
WO
WIPO (PCT)
Prior art keywords
droplet
mass spectrometry
sample preparation
chip sample
chip
Prior art date
Application number
PCT/US2006/021699
Other languages
French (fr)
Other versions
WO2007136386A2 (en
Inventor
Aaron R Wheeler
Robin L Garrell
Chang-Jin Kim
Hyejin Moon
Original Assignee
Univ California
Aaron R Wheeler
Robin L Garrell
Chang-Jin Kim
Hyejin Moon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Aaron R Wheeler, Robin L Garrell, Chang-Jin Kim, Hyejin Moon filed Critical Univ California
Publication of WO2007136386A2 publication Critical patent/WO2007136386A2/en
Publication of WO2007136386A3 publication Critical patent/WO2007136386A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0678—Facilitating or initiating evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4022—Concentrating samples by thermal techniques; Phase changes
    • G01N2001/4027—Concentrating samples by thermal techniques; Phase changes evaporation leaving a concentrated sample

Abstract

The present invention relates to droplet-based on-chip sample preparation for mass spectrometry. Specifically, sample processing is performed in matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) using one or more droplet-based microfluidic devices to manipulate droplets containing samples and reagents and place them to specified locations on an array.
PCT/US2006/021699 2005-06-06 2006-06-06 Droplet-based on-chip sample preparation for mass spectrometry WO2007136386A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68766205P 2005-06-06 2005-06-06
US60/687,662 2005-06-06

Publications (2)

Publication Number Publication Date
WO2007136386A2 WO2007136386A2 (en) 2007-11-29
WO2007136386A3 true WO2007136386A3 (en) 2008-11-27

Family

ID=38723728

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/021699 WO2007136386A2 (en) 2005-06-06 2006-06-06 Droplet-based on-chip sample preparation for mass spectrometry

Country Status (1)

Country Link
WO (1) WO2007136386A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883513B2 (en) 2006-04-18 2014-11-11 Advanced Liquid Logic, Inc. Droplet-based particle sorting

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US8367370B2 (en) 2008-02-11 2013-02-05 Wheeler Aaron R Droplet-based cell culture and cell assays using digital microfluidics
US8187864B2 (en) 2008-10-01 2012-05-29 The Governing Council Of The University Of Toronto Exchangeable sheets pre-loaded with reagent depots for digital microfluidics
US8053239B2 (en) 2008-10-08 2011-11-08 The Governing Council Of The University Of Toronto Digital microfluidic method for protein extraction by precipitation from heterogeneous mixtures
CA2740113C (en) 2008-10-10 2019-12-24 The Governing Council Of The University Of Toronto Hybrid digital and channel microfluidic devices and methods of use thereof
CH700127A1 (en) * 2008-12-17 2010-06-30 Tecan Trading Ag System and apparatus for processing biological samples and for manipulating liquids with biological samples.
US9851365B2 (en) 2009-02-26 2017-12-26 The Governing Council Of The University Of Toronto Digital microfluidic liquid-liquid extraction device and method of use thereof
US8202736B2 (en) 2009-02-26 2012-06-19 The Governing Council Of The University Of Toronto Method of hormone extraction using digital microfluidics
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
GB201011019D0 (en) 2010-07-01 2010-08-18 Weston Richard Ionisation mass spectrometry
CA2813090C (en) 2010-10-01 2019-11-12 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase
FR2967148B1 (en) * 2010-11-10 2012-12-21 Commissariat Energie Atomique CONTROLLED EVAPORATION METHOD OF A LIQUID DROP IN A MICROFLUIDIC DEVICE
US20160115436A1 (en) * 2013-05-10 2016-04-28 The Regents Of The University Of California Digital microfluidic platform for creating, maintaining and analyzing 3-dimensional cell spheroids
KR101828926B1 (en) * 2013-12-18 2018-02-13 도요세이칸 그룹 홀딩스 가부시키가이샤 Culture container, method for culturing lymphocytes, culture-container production method, and solid-phasing apparatus
CA2981515A1 (en) 2015-04-03 2016-10-06 Abbott Laboratories Devices and methods for sample analysis
MX2017012742A (en) 2015-04-03 2017-11-15 Abbott Lab Devices and methods for sample analysis.
CN108026494A (en) 2015-06-05 2018-05-11 米罗库鲁斯公司 Limitation evaporation and the digital microcurrent-controlled apparatus and method of air matrix of surface scale
WO2016197106A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Evaporation management in digital microfluidic devices
WO2017004463A1 (en) 2015-07-01 2017-01-05 Abbott Laboratories Devices and methods for sample analysis
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
WO2018067878A1 (en) 2016-10-05 2018-04-12 Abbott Laboratories Devices and methods for sample analysis
WO2018126082A1 (en) 2016-12-28 2018-07-05 Miroculis Inc. Digital microfluidic devices and methods
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
CN110892258A (en) 2017-07-24 2020-03-17 米罗库鲁斯公司 Digital microfluidic system and method with integrated plasma collection device
CN109420531B (en) * 2017-08-31 2020-02-21 厦门大学 Microfluidic chip and method for storing and releasing reagents thereof
CN111587149B (en) 2017-09-01 2022-11-11 米罗库鲁斯公司 Digital microfluidic device and method of use thereof
EP3788371A1 (en) 2018-05-04 2021-03-10 Abbott Laboratories Sequential sampling method for improving immunoassay sensitivity and kinetics of small volume samples
CN113454455A (en) 2018-12-28 2021-09-28 雅培制药有限公司 Direct detection of single molecules on microparticles
WO2020210292A1 (en) 2019-04-08 2020-10-15 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
JP2022543618A (en) 2019-08-07 2022-10-13 アボット・ラボラトリーズ Chemiluminescent compounds for multiplexing
US20220276256A1 (en) 2019-08-07 2022-09-01 Abbott Laboratories Methods for detecting assay interferents and increasing dynamic range
CN110918146B (en) * 2019-12-19 2021-07-09 上海天马微电子有限公司 Micro-fluidic panel
CN110918145B (en) * 2019-12-19 2021-07-09 上海天马微电子有限公司 Microfluidic panel and driving method thereof
US20210205813A1 (en) * 2019-12-23 2021-07-08 National Technology & Engineering Solutions Of Sandia, Llc Contactless liquid loading to microfluidic devices
CN111054455B (en) * 2019-12-26 2021-07-09 上海天马微电子有限公司 Microfluidic chip and driving method thereof
WO2023122232A1 (en) * 2021-12-23 2023-06-29 Amgen Inc. Methods for detecting a protein in a sample in a fluidic device using mass spectrometry
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Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
US20040058450A1 (en) * 2002-09-24 2004-03-25 Pamula Vamsee K. Methods and apparatus for manipulating droplets by electrowetting-based techniques

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883513B2 (en) 2006-04-18 2014-11-11 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US9395329B2 (en) 2006-04-18 2016-07-19 Advanced Liquid Logic, Inc. Droplet-based particle sorting

Also Published As

Publication number Publication date
WO2007136386A2 (en) 2007-11-29

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