WO2008008484A3 - Detection of truncation mutations by mass spectrometry - Google Patents

Detection of truncation mutations by mass spectrometry Download PDF

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Publication number
WO2008008484A3
WO2008008484A3 PCT/US2007/015977 US2007015977W WO2008008484A3 WO 2008008484 A3 WO2008008484 A3 WO 2008008484A3 US 2007015977 W US2007015977 W US 2007015977W WO 2008008484 A3 WO2008008484 A3 WO 2008008484A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
mass spectrometry
truncation mutations
terminal
proteins
Prior art date
Application number
PCT/US2007/015977
Other languages
French (fr)
Other versions
WO2008008484A2 (en
Inventor
Alex M Garvin
Sadanand Gite
Vladislav Bergo
Kenneth Rothschild
Original Assignee
Ambergen Inc
Alex M Garvin
Sadanand Gite
Vladislav Bergo
Kenneth Rothschild
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambergen Inc, Alex M Garvin, Sadanand Gite, Vladislav Bergo, Kenneth Rothschild filed Critical Ambergen Inc
Publication of WO2008008484A2 publication Critical patent/WO2008008484A2/en
Publication of WO2008008484A3 publication Critical patent/WO2008008484A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Abstract

This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated with cell-free translation systems using isotopically-depleted amino acids. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.
PCT/US2007/015977 2006-07-14 2007-07-12 Detection of truncation mutations by mass spectrometry WO2008008484A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/487,128 2006-07-14
US11/487,128 US7563598B2 (en) 2004-07-30 2006-07-14 Detection of truncation mutations by mass spectrometry

Publications (2)

Publication Number Publication Date
WO2008008484A2 WO2008008484A2 (en) 2008-01-17
WO2008008484A3 true WO2008008484A3 (en) 2008-11-27

Family

ID=38923928

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/015977 WO2008008484A2 (en) 2006-07-14 2007-07-12 Detection of truncation mutations by mass spectrometry

Country Status (2)

Country Link
US (1) US7563598B2 (en)
WO (1) WO2008008484A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110045991A1 (en) * 2005-06-23 2011-02-24 Sadanand Gite Methods for the Detection of Colorectal Cancer
US8114587B2 (en) * 2005-06-23 2012-02-14 Ambergen, Inc. Methods for the detection of colorectal cancer
ES2541178T3 (en) 2009-02-03 2015-07-16 Mdxhealth Sa Colorectal Cancer Screening Method
JP5201218B2 (en) * 2009-06-15 2013-06-05 トヨタ自動車株式会社 Cell-free protein synthesis solution, cell-free protein synthesis kit and protein synthesis method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6322970B1 (en) * 1997-09-02 2001-11-27 Sequenom, Inc. Mass spectrometric detection of polypeptides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6322970B1 (en) * 1997-09-02 2001-11-27 Sequenom, Inc. Mass spectrometric detection of polypeptides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARSHALL A.G. ET AL.: "Proten Molecular Mass to 1 Da by 13C, 15N Double-Depletion and FT-ICR Mass Spectrometry", J. AM. CHEM. SOC., vol. 119, 1997, pages 433 - 434 *
SHIMIZU Y. ET AL.: "Cell-free translation reconstituted with purified components", NATURE BIOTECHNOLOGY, vol. 19, August 2001 (2001-08-01), pages 751 - 755, XP001060378 *
SPIRIN A.S.: "High-throughput-cell-free systes for synthesis of functionally active proteins", TRENDS BIOTECHNOL., vol. 22, no. 10, October 2004 (2004-10-01), pages 538 - 545, XP004575232 *

Also Published As

Publication number Publication date
US7563598B2 (en) 2009-07-21
US20070072214A1 (en) 2007-03-29
WO2008008484A2 (en) 2008-01-17

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