WO2008021653A3 - Method of measurement of cellular hemoglobin - Google Patents

Method of measurement of cellular hemoglobin Download PDF

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Publication number
WO2008021653A3
WO2008021653A3 PCT/US2007/073870 US2007073870W WO2008021653A3 WO 2008021653 A3 WO2008021653 A3 WO 2008021653A3 US 2007073870 W US2007073870 W US 2007073870W WO 2008021653 A3 WO2008021653 A3 WO 2008021653A3
Authority
WO
WIPO (PCT)
Prior art keywords
hemoglobin
red blood
reticulocytes
cellular
cell
Prior art date
Application number
PCT/US2007/073870
Other languages
French (fr)
Other versions
WO2008021653A2 (en
Inventor
Agthoven Andreas Van
Fabrice Malergue
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Priority to JP2009523878A priority Critical patent/JP5362564B2/en
Priority to EP07813098A priority patent/EP2047264A4/en
Priority to CN200780029610.2A priority patent/CN101501497B/en
Publication of WO2008021653A2 publication Critical patent/WO2008021653A2/en
Publication of WO2008021653A3 publication Critical patent/WO2008021653A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/726—Devices
    • G01N2015/014—

Abstract

A method of measuring cellular hemoglobin of a blood sample includes mixing a blood sample with a permeation reagent, and incubating the sample mixture to permeate cellular membrane of red blood cells and to cause hemoglobin aggregation within the cells; adding a neutralization reagent to inhibit further reactions of the permeation reagent; performing a cell-by-cell measurement of side scatter signals of the red blood cells in the sample mixture on a flow cytometer; and obtaining cellular hemoglobin (Hgbcell) of each red blood cell using the obtained side scatter signals. The method further includes measuring cellular hemoglobin of reticulocytes (Hgbretic) by differentiating reticulocytes using a simultaneous fluorescence measurement. The method also includes measuring cellular percentage of a hemoglobin variant in mature red blood cells or reticulocytes by adding a fluorescent antibody in the neutralization reagent and detecting fluorescence signals of antibody bound hemoglobin variant.
PCT/US2007/073870 2006-08-09 2007-07-19 Method of measurement of cellular hemoglobin WO2008021653A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009523878A JP5362564B2 (en) 2006-08-09 2007-07-19 Method for measuring cellular hemoglobin
EP07813098A EP2047264A4 (en) 2006-08-09 2007-07-19 Method of measurement of cellular hemoglobin
CN200780029610.2A CN101501497B (en) 2006-08-09 2007-07-19 The method of testing of cellular hemoglobin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/501,395 US7541190B2 (en) 2005-02-07 2006-08-09 Method of measurement of cellular hemoglobin
US11/501,395 2006-08-09

Publications (2)

Publication Number Publication Date
WO2008021653A2 WO2008021653A2 (en) 2008-02-21
WO2008021653A3 true WO2008021653A3 (en) 2008-10-23

Family

ID=39082850

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/073870 WO2008021653A2 (en) 2006-08-09 2007-07-19 Method of measurement of cellular hemoglobin

Country Status (5)

Country Link
US (2) US7541190B2 (en)
EP (2) EP2500727A1 (en)
JP (1) JP5362564B2 (en)
CN (1) CN101501497B (en)
WO (1) WO2008021653A2 (en)

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US7968279B2 (en) * 2008-08-13 2011-06-28 Beckman Coulter, Inc. Reference control for cell by cell analysis
US8603773B2 (en) * 2008-09-19 2013-12-10 Beckman Coulter Method and system for analyzing a blood sample
CA2806261A1 (en) * 2010-08-11 2012-02-16 Kyowa Medex Co., Ltd. Method for measuring glycated hemoglobin
CN103874913B (en) * 2011-06-17 2017-04-26 罗氏血液诊断股份有限公司 Solutions for histoprocessing of biological samples
EP2972315B1 (en) 2013-03-12 2020-01-15 Abbott Laboratories System for optical analysis of the mean corpuscular volume of red blood cells
CN104458541A (en) * 2013-09-12 2015-03-25 深圳迈瑞生物医疗电子股份有限公司 Analysis method for red blood cell hemoglobin content, device and blood cell analyzer
EA035272B1 (en) * 2014-02-26 2020-05-22 Брайгхэм Энд Вимен'С Хоспитэл, Инк. System and method for cell levitation and monitoring
WO2016013115A1 (en) * 2014-07-25 2016-01-28 株式会社 リージャー Analysis method for diluted biological sample component
FR3024778B1 (en) * 2014-08-05 2021-01-22 Screencell PROCEDURE FOR SCREENING FOR DREPANOCYTOSIS AND KIT FOR ITS IMPLEMENTATION
CN106716138B (en) * 2014-09-12 2019-06-14 贝克曼考尔特公司 System and method for determining Cell Age
WO2016152672A1 (en) * 2015-03-25 2016-09-29 富士フイルム株式会社 Method for isolating nucleated red blood cell candidate cells
CN106018053B (en) * 2016-07-01 2019-04-30 安邦（厦门）生物科技有限公司 Glycosylated hemoglobin detection micro red blood cell collection device and acquisition testing method
FR3058525B1 (en) * 2016-11-04 2021-02-12 Univ Paris Val De Marne METHOD FOR DETERMINING THE HEMOGLOBIN F CONTENT OF AN ERYTHROID CELL
CN110140051B (en) * 2017-01-05 2021-03-05 深圳迈瑞生物医疗电子股份有限公司 Reticulocyte mimic particle, platelet mimic particle preparation method and quality control material
KR20180091175A (en) 2017-02-06 2018-08-16 삼성전자주식회사 Fluid analysis cartridge and fluid analysis cartridge assembly having the same
CA3063144C (en) * 2017-05-08 2022-06-14 Beckman Coulter, Inc. Compositions and methods for lysis of red blood cells
WO2019060687A1 (en) 2017-09-21 2019-03-28 Beckman Coulter, Inc. Method of determining fetal red blood cells in maternal circulation using flow cytometry
WO2019206297A1 (en) * 2018-04-28 2019-10-31 深圳迈瑞生物医疗电子股份有限公司 Blood analyzer and analysis method
CN110286080A (en) * 2018-10-25 2019-09-27 中国科学院苏州生物医学工程技术研究所 Ejaculated sperm cells rapid typing detection reagent box and detection method
CN111504887B (en) * 2020-05-09 2023-05-09 苏州四正柏生物科技有限公司 Hemolysin and preparation method thereof
CN111721950A (en) * 2020-05-29 2020-09-29 迪瑞医疗科技股份有限公司 Stable formed component analysis coking liquid and preparation method thereof
WO2022032683A1 (en) * 2020-08-14 2022-02-17 深圳迈瑞生物医疗电子股份有限公司 Blood analyzer and hemoglobin measurement method
CN112305210B (en) * 2020-10-15 2023-06-16 爱若维生物科技(苏州)有限公司 Three-classification blood cell analysis reagent for livestock and preparation method thereof

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Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
EP0617281B1 (en) * 1993-03-19 1999-06-16 Sysmex Corporation A reagent for measuring immature leukocytes
US6534279B1 (en) * 1998-07-09 2003-03-18 Immunotech Reagent and method for the permeabilization and identification of erythrocytes
US6271035B1 (en) * 1998-10-20 2001-08-07 Coulter International Corp. Methods and compositions for rapid staining of nucleic acids in whole cells
US20030025896A1 (en) * 2001-06-05 2003-02-06 Oever Ronny Van?Apos;T Optical method and apparatus for red blood cell differentiation on a cell-by-cell basis, and simultaneous analysis of white blood cell differentiation
US20040214243A1 (en) * 2003-04-25 2004-10-28 Beckman Coulter, Inc. Differential determination of hemoglobins

Also Published As

Publication number Publication date
JP2010500570A (en) 2010-01-07
EP2500727A1 (en) 2012-09-19
EP2047264A2 (en) 2009-04-15
CN101501497B (en) 2017-06-09
CN101501497A (en) 2009-08-05
JP5362564B2 (en) 2013-12-11
EP2047264A4 (en) 2010-04-21
WO2008021653A2 (en) 2008-02-21
US20070020612A1 (en) 2007-01-25
US7541190B2 (en) 2009-06-02
US20090220936A1 (en) 2009-09-03

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