WO2008122051B1 - Methods and devices for enhanced analysis of field focused cells and particles - Google Patents

Methods and devices for enhanced analysis of field focused cells and particles

Info

Publication number
WO2008122051B1
WO2008122051B1 PCT/US2008/059181 US2008059181W WO2008122051B1 WO 2008122051 B1 WO2008122051 B1 WO 2008122051B1 US 2008059181 W US2008059181 W US 2008059181W WO 2008122051 B1 WO2008122051 B1 WO 2008122051B1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
particles
population
channel
acoustically
Prior art date
Application number
PCT/US2008/059181
Other languages
French (fr)
Other versions
WO2008122051A1 (en
Inventor
Gregory Kaduchak
Michael D Ward
Original Assignee
Acoustic Cytometry Systems Inc
Gregory Kaduchak
Michael D Ward
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AT08733084T priority Critical patent/ATE538377T1/en
Priority to EP08733084A priority patent/EP2156178B1/en
Application filed by Acoustic Cytometry Systems Inc, Gregory Kaduchak, Michael D Ward filed Critical Acoustic Cytometry Systems Inc
Priority to US12/239,410 priority patent/US8865476B2/en
Priority to US12/239,483 priority patent/US8900870B2/en
Priority to US12/239,453 priority patent/US8846408B2/en
Priority to US12/239,390 priority patent/US8227257B2/en
Priority to US12/239,467 priority patent/US8134705B2/en
Priority to US12/239,513 priority patent/US8309408B2/en
Priority to US12/239,501 priority patent/US9457139B2/en
Publication of WO2008122051A1 publication Critical patent/WO2008122051A1/en
Publication of WO2008122051B1 publication Critical patent/WO2008122051B1/en
Priority to US13/365,375 priority patent/US8436993B2/en
Priority to US13/523,370 priority patent/US8507293B2/en
Priority to US13/614,956 priority patent/US9134271B2/en
Priority to US13/849,456 priority patent/US8873051B2/en
Priority to US14/464,763 priority patent/US9476855B2/en
Priority to US15/239,948 priority patent/US10254212B2/en
Priority to US16/282,793 priority patent/US10969325B2/en
Priority to US17/186,296 priority patent/US20210364407A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3678—Separation of cells using wave pressure; Manipulation of individual corpuscles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/12—Coulter-counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/22—Details, e.g. general constructional or apparatus details
    • G01N29/222—Constructional or flow details for analysing fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N15/1409—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/1415—Control of particle position
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/142—Acoustic or ultrasonic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/1702—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/024—Mixtures
    • G01N2291/02416—Solids in liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/028—Material parameters
    • G01N2291/02809—Concentration of a compound, e.g. measured by a surface mass change
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/028—Material parameters
    • G01N2291/02836—Flow rate, liquid level
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25625—Dilution
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

A system and method for analyzing one or more particles in a flow cell comprises focusing the one or more particles in a fluid stream with an acoustic standing wave to acoustically focus the one or more particles in a flow cell. The fluid is transited within the flow cell with a linear flow velocity ranging between about 0 meters/second and about 10 meters/second in the forward or reverse direction.

Claims

AMENDED CLAIMS received by the International Bureau on 23 September 2008 (23.09.08)CLAIMSWhat is claimed is:
1. A method for acoustically reorienting a fluid in a channel comprising; introducing into a channel a first fluid wherein the first fluid has a first acoustic contrast relative to a second fluid; introducing into the channel the second fluid wherein the second fluid has a second acoustic contrast that is different from the first acoustic contrast; applying acoustic radiation pressure to the channel; acoustically reorienting the second fluid based upon the acoustic contrast of the second fluid.
2. The method of claim 1 further comprising moving the first fluid in a laminar flow stream.
3. The method of claim 2 further comprising moving the second fluid in a second laminar flow stream parallel to a flow stream of the first fluid.
4. The method of claim 1 wherein the second fluid comprises particles therein.
5. The method of claim 4 wherein the first fluid and the second fluid are acoustically reoriented relative to the particles.
6. The method of claim 5 further comprising assaying the particles or fluids to produce an assay,
7. An assay produced by the method of claim 6 wherein the second fluid is a biological fluid.
8. The method of claim 1 wherein the first fluid comprises particles therein.
9. The method of claim 1 wherein the first fluid or the second fluid is acoustically reoriented based upon the first acoustic contrast or the second acoustic contrast.
10. The method of claim 1 further comprising flowing the particles in the second fluid to a particle analyzer in fine with the channel.
11. The method of claim 1 wherein the first fluid or the second fluid comprises reagents.
12. A method for acoustically manipulating a particle using radial acoustic focusing comprising: introducing into a channel a first fluid having a first acoustic contrast with a population of particles suspended therein; introducing into the channel a second fluid having a second acoustic contrast that is greater than or equal to the acoustic contrast of the first fluid; applying a radial acoustic radiation pressure to the channel; and acoustically focusing at least a portion of the population of particles from the first fluid to the second fluid.
13. The method of claim 12 wherein the portion of the population of particles are acoustically focused relative to the first acoustic contrast and the second acoustic contrast.
14. The method of claim 12 further comprising moving the first fluid in a first laminar flow stream and moving the second fluid in a second laminar flow stream.
15. The method of claim 12 wherein the step of acoustically focusing comprises concentrating the population of particles into the second stream.
16. The method of claim 12 further comprising analyzing in a particle analyzer the portion of the population of particles in the second fluid.
17. The method of claim 12 further comprising imaging with an imager the portion of the population of particles in the second fluid.
18. The method of claim 12 further comprising non-dilutive sorting of the portion of the population of particles in the second fluid,
19. The method of claim 12 wherein the first fluid comprises a reagent that reacts with at least some of the population of particles.
20. The method of claim 12 wherein the second fluid is located at a center of the channel.
21. The method of claim 12 wherein the population of particles is selected from the group consisting of cells, beads, or any combination thereof.
22. The method of claim 12 further comprising flowing the particle in the first fluid or the second fluid to a particle analyzer that is in line with the channel.
23. The method of claim 22 wherein the particle analyzer is a flow cytometer.
24. The method of claim 12 wherein the population of particles are cultured cells, the first fluid is cell growth medium in which the cells are grown and the second fluid is new cell growth medium.
25. The method of claim 12 wherein the population of particles are microsphere beads.
26. The method of claim 12 wherein a negative contrast particle is within the population of particles.
27. The method of claim 26 further comprising: capturing a particle of interest from the population of particles with the negative contrast particle; and forcing the particle of interest and the negative contrast particle toward a wall of the channel away from a center of the channel.
28. The method of claim 12 wherein the population of particles is blood cells.
29. The method of claim 28 further comprising assaying the particles or fluids to produce an assay.
30. A method for imaging an acoustically manipulated rare target in an acoustic flow cytorneter comprising: introducing a fluid comprising the rare target to a flow cell; applying acoustic radiation pressure to the flow cell; acoustically focusing the rare target within the flow cell to concentrate the rare target; aligning the concentrated rare target in the flow eel); interrogating the aligned rare target at an interrogation site to obtain an optical signal from the rare target to yield population statistical data: and imaging the rare target to produce a high content image representative of the rare target.
31. The method of claim 30 further comprising correlating the population statistical data with the high content image to produce improved data content.
32. The method of claim 30 further comprising slowing the transit time of the rare target during the interrogating step.
PCT/US2008/059181 2007-04-02 2008-04-02 Methods and devices for enhanced analysis of field focused cells and particles WO2008122051A1 (en)

Priority Applications (17)

Application Number Priority Date Filing Date Title
AT08733084T ATE538377T1 (en) 2007-04-02 2008-04-02 METHOD FOR IMPROVED ANALYSIS OF CELLS AND PARTICLES FOCUSED IN AN ACOUSTIC FIELD
EP08733084A EP2156178B1 (en) 2007-04-02 2008-04-02 Methods for enhanced analysis of acoustic field focused cells and particles
US12/239,410 US8865476B2 (en) 2007-04-02 2008-09-26 Particle switching systems and methods using acoustic radiation pressure
US12/239,483 US8900870B2 (en) 2007-04-02 2008-09-26 Methods for fusing cells using acoustic radiation pressure
US12/239,453 US8846408B2 (en) 2007-04-02 2008-09-26 Particle analyzing systems and methods using acoustic radiation pressure
US12/239,390 US8227257B2 (en) 2007-04-02 2008-09-26 Medium switching systems and methods using acoustic radiation pressure
US12/239,467 US8134705B2 (en) 2007-04-02 2008-09-26 Particle imaging systems and methods using acoustic radiation pressure
US12/239,513 US8309408B2 (en) 2007-04-02 2008-09-26 Particle quantifying systems and methods using acoustic radiation pressure
US12/239,501 US9457139B2 (en) 2007-04-02 2008-09-26 Kits for systems and methods using acoustic radiation pressure
US13/365,375 US8436993B2 (en) 2007-04-02 2012-02-03 Methods and systems for controlling the flow of particles for detection
US13/523,370 US8507293B2 (en) 2007-04-02 2012-06-14 Medium switching systems and methods using acoustic radiation pressure
US13/614,956 US9134271B2 (en) 2007-04-02 2012-09-13 Particle quantifying systems and methods using acoustic radiation pressure
US13/849,456 US8873051B2 (en) 2007-04-02 2013-03-22 Methods and systems for controlling the flow of particles for detection
US14/464,763 US9476855B2 (en) 2007-04-02 2014-08-21 Particle analyzing systems and methods using acoustic radiation pressure
US15/239,948 US10254212B2 (en) 2007-04-02 2016-08-18 Particle analyzing systems and methods using acoustic radiation pressure
US16/282,793 US10969325B2 (en) 2007-04-02 2019-02-22 Particle analyzing systems and methods using acoustic radiation pressure
US17/186,296 US20210364407A1 (en) 2007-04-02 2021-02-26 Particle Analyzing Systems And Methods Using Acoustic Radiation Pressure

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US90970407P 2007-04-02 2007-04-02
US60/909,704 2007-04-02
US2608208P 2008-02-04 2008-02-04
US61/026,082 2008-02-04

Related Child Applications (7)

Application Number Title Priority Date Filing Date
US12/239,453 Continuation-In-Part US8846408B2 (en) 2007-04-02 2008-09-26 Particle analyzing systems and methods using acoustic radiation pressure
US12/239,483 Continuation-In-Part US8900870B2 (en) 2007-04-02 2008-09-26 Methods for fusing cells using acoustic radiation pressure
US12/239,410 Continuation-In-Part US8865476B2 (en) 2007-04-02 2008-09-26 Particle switching systems and methods using acoustic radiation pressure
US12/239,513 Continuation-In-Part US8309408B2 (en) 2007-04-02 2008-09-26 Particle quantifying systems and methods using acoustic radiation pressure
US12/239,390 Continuation-In-Part US8227257B2 (en) 2007-04-02 2008-09-26 Medium switching systems and methods using acoustic radiation pressure
US12/239,467 Continuation-In-Part US8134705B2 (en) 2007-04-02 2008-09-26 Particle imaging systems and methods using acoustic radiation pressure
US12/239,501 Continuation-In-Part US9457139B2 (en) 2007-04-02 2008-09-26 Kits for systems and methods using acoustic radiation pressure

Publications (2)

Publication Number Publication Date
WO2008122051A1 WO2008122051A1 (en) 2008-10-09
WO2008122051B1 true WO2008122051B1 (en) 2008-11-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/059181 WO2008122051A1 (en) 2007-04-02 2008-04-02 Methods and devices for enhanced analysis of field focused cells and particles

Country Status (4)

Country Link
US (15) US9457139B2 (en)
EP (3) EP2664916B1 (en)
AT (1) ATE538377T1 (en)
WO (1) WO2008122051A1 (en)

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