WO2008134069A3 - Anchored ligand agents - Google Patents
Anchored ligand agents Download PDFInfo
- Publication number
- WO2008134069A3 WO2008134069A3 PCT/US2008/005528 US2008005528W WO2008134069A3 WO 2008134069 A3 WO2008134069 A3 WO 2008134069A3 US 2008005528 W US2008005528 W US 2008005528W WO 2008134069 A3 WO2008134069 A3 WO 2008134069A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ligand
- target
- target molecule
- binding
- active site
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
Abstract
The invention provides novel therapeutic agents comprising ligand molecules such as aptamers linked to a molecular anchor (MA). In the absence of a MA, ligands rapidly diffuse into bulk solution after dissociation from a target molecule. With a MA tightly bound to the target molecule, anchored ligand molecules of the invention remain in close proximity to the active site on the target molecule, even after dissociating from the target, permitting rapid re- binding of ligand to its target. Reversibility of binding to the active site can be effectively and rapidly achieved using antidotes based on the complementary DNA sequences. A preferred embodiment in accordance with the invention is a highly efficient anticoagulant agent that demonstrates binding efficiency to thrombin that is increased many fold relative to a corresponding unanchored aptamer-based ligand.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US91424207P | 2007-04-26 | 2007-04-26 | |
US60/914,242 | 2007-04-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008134069A2 WO2008134069A2 (en) | 2008-11-06 |
WO2008134069A3 true WO2008134069A3 (en) | 2009-02-12 |
Family
ID=39926277
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/005528 WO2008134069A2 (en) | 2007-04-26 | 2008-04-28 | Anchored ligand agents |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2008134069A2 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5756291A (en) * | 1992-08-21 | 1998-05-26 | Gilead Sciences, Inc. | Aptamers specific for biomolecules and methods of making |
US20020037506A1 (en) * | 2000-04-18 | 2002-03-28 | Yun Lin | Aptamer based two-site binding assay |
US20030175703A1 (en) * | 2000-09-26 | 2003-09-18 | Sullenger Bruce A. | RNA aptamers and methods for identifying the same |
US20050123936A1 (en) * | 2003-01-16 | 2005-06-09 | Ansari Aseem Z. | Method, composition, and kit to design, evaluate, and/or test compounds that modulate regulatory factor binding to nucleic acids |
WO2007025049A2 (en) * | 2005-08-26 | 2007-03-01 | Archemix Corp. | Aptamers that bind thrombin with high affinity |
-
2008
- 2008-04-28 WO PCT/US2008/005528 patent/WO2008134069A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5756291A (en) * | 1992-08-21 | 1998-05-26 | Gilead Sciences, Inc. | Aptamers specific for biomolecules and methods of making |
US20020037506A1 (en) * | 2000-04-18 | 2002-03-28 | Yun Lin | Aptamer based two-site binding assay |
US20030175703A1 (en) * | 2000-09-26 | 2003-09-18 | Sullenger Bruce A. | RNA aptamers and methods for identifying the same |
US20050123936A1 (en) * | 2003-01-16 | 2005-06-09 | Ansari Aseem Z. | Method, composition, and kit to design, evaluate, and/or test compounds that modulate regulatory factor binding to nucleic acids |
WO2007025049A2 (en) * | 2005-08-26 | 2007-03-01 | Archemix Corp. | Aptamers that bind thrombin with high affinity |
Also Published As
Publication number | Publication date |
---|---|
WO2008134069A2 (en) | 2008-11-06 |
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