WO2009111067A3 - Methods for the identification of agents that inhibit mesenchymal -like tumor cells or their formation - Google Patents

Methods for the identification of agents that inhibit mesenchymal -like tumor cells or their formation Download PDF

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Publication number
WO2009111067A3
WO2009111067A3 PCT/US2009/001456 US2009001456W WO2009111067A3 WO 2009111067 A3 WO2009111067 A3 WO 2009111067A3 US 2009001456 W US2009001456 W US 2009001456W WO 2009111067 A3 WO2009111067 A3 WO 2009111067A3
Authority
WO
WIPO (PCT)
Prior art keywords
emt
agents
tumor cell
inhibit
identification
Prior art date
Application number
PCT/US2009/001456
Other languages
French (fr)
Other versions
WO2009111067A2 (en
WO2009111067A9 (en
Inventor
John D. Haley
Stuart Thomson
Julie Kan
Salam A. Shaaban
Original Assignee
Osi Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osi Pharmaceuticals, Inc. filed Critical Osi Pharmaceuticals, Inc.
Priority to JP2010549675A priority Critical patent/JP2011516826A/en
Priority to EP09716601A priority patent/EP2271941B1/en
Publication of WO2009111067A2 publication Critical patent/WO2009111067A2/en
Publication of WO2009111067A3 publication Critical patent/WO2009111067A3/en
Publication of WO2009111067A9 publication Critical patent/WO2009111067A9/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/485—Epidermal growth factor [EGF] (urogastrone)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/495—Transforming growth factor [TGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G01N2333/525—Tumor necrosis factor [TNF]

Abstract

The present invention provides tumor cell preparations for use as models of the EMT process for use in the identification of anti-cancer agents, wherein said tumor cell preparations comprise cells of the epithelial tumor cell line H358, which are stimulated by receptor ligands to induce EMT, or which have been engineered to inducibly express a protein that stimulates EMT. The present invention also provides methods of identifying potential anti-cancer agents by using such tumor cell preparations to identify agents that inhibit EMT, stimulate MET, or inhibit the growth of mesenchymal-like cells. Such agents should be particularly useful when used in conjunction with other anti-cancer drugs such as EGFR and IGF-IR kinase inhibitors, which appear to be less effective at inhibiting tumor cells that have undergone an EMT.
PCT/US2009/001456 2008-03-07 2009-03-06 Methods for the identification of agents that inhibit mesenchymal -like tumor cells or their formation WO2009111067A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2010549675A JP2011516826A (en) 2008-03-07 2009-03-06 Methods for identification of mesenchymal-like tumor cells or agents that inhibit this formation
EP09716601A EP2271941B1 (en) 2008-03-07 2009-03-06 Methods for the identification of agents that inhibit mesenchymal-like tumor cells or their formation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6861208P 2008-03-07 2008-03-07
US61/068,612 2008-03-07

Publications (3)

Publication Number Publication Date
WO2009111067A2 WO2009111067A2 (en) 2009-09-11
WO2009111067A3 true WO2009111067A3 (en) 2009-11-05
WO2009111067A9 WO2009111067A9 (en) 2009-12-23

Family

ID=40791606

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/001456 WO2009111067A2 (en) 2008-03-07 2009-03-06 Methods for the identification of agents that inhibit mesenchymal -like tumor cells or their formation

Country Status (4)

Country Link
US (1) US7951549B2 (en)
EP (1) EP2271941B1 (en)
JP (1) JP2011516826A (en)
WO (1) WO2009111067A2 (en)

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WO2010099137A2 (en) * 2009-02-26 2010-09-02 Osi Pharmaceuticals, Inc. In situ methods for monitoring the emt status of tumor cells in vivo
EP2401613A2 (en) * 2009-02-27 2012-01-04 OSI Pharmaceuticals, LLC Methods for the identification of agents that inhibit mesenchymal-like tumor cells or their formation
US8642834B2 (en) * 2009-02-27 2014-02-04 OSI Pharmaceuticals, LLC Methods for the identification of agents that inhibit mesenchymal-like tumor cells or their formation
US20110143960A1 (en) * 2009-12-10 2011-06-16 Labarbera Daniel V 3d-models for high-throughput screening drug discovery and development
EP4190149A1 (en) * 2009-12-25 2023-06-07 Chugai Seiyaku Kabushiki Kaisha Method for searching and screening for target of anti-cancer agent using non-human animal model having nog established cancer cell line transplanted therein
TWI606835B (en) * 2010-04-23 2017-12-01 中央研究院 Reishi polysaccharide-based compositions and methods for treatment of cancer
SG189302A1 (en) 2010-10-06 2013-05-31 Pharmalogicals Res Pte Ltd Cancer stem cell population and method for production thereof
WO2012061515A2 (en) * 2010-11-03 2012-05-10 Merck Sharp & Dohme Corp. Methods of classifying human subjects with regard to cancer prognosis
EP2749641B1 (en) 2011-09-07 2021-06-02 Chugai Seiyaku Kabushiki Kaisha Cancer stem cell isolation
SG11201400919RA (en) * 2011-09-23 2014-10-30 Agency Science Tech & Res Patient stratification and determining clinical outcome for cancer patients
WO2013062083A1 (en) 2011-10-28 2013-05-02 ファーマロジカルズ・リサーチ プライベート リミテッド Cancer stem cell-specific molecule
JP2014033626A (en) * 2012-08-08 2014-02-24 Juntendo Method for creating model mouse for distant metastasis of human cancer epithelial cell
EP2894472A4 (en) * 2012-09-05 2016-03-30 Jsr Corp Method for screening matter acting on epithelial maintenance of cells
WO2014043633A1 (en) * 2012-09-17 2014-03-20 Agios Pharmaceuticals, Inc. Use of e-cadherin and vimentin for selection of treatment responsive patients
CA2892089A1 (en) 2012-11-21 2014-05-30 Rene M. Lemieux Glutaminase inhibitors and methods of use
WO2014079011A1 (en) 2012-11-22 2014-05-30 Agios Pharmaceuticals, Inc. Heterocyclic compounds for inhibiting glutaminase and their methods of use
US9029531B2 (en) 2012-11-22 2015-05-12 Agios Pharmaceuticals, Inc. Compounds and their methods of use
JP2016522675A (en) * 2013-03-14 2016-08-04 ガラパゴス・ナムローゼ・フェンノートシャップＧａｌａｐａｇｏｓ Ｎ．Ｖ． Molecular targets useful in the treatment of diseases associated with epithelial-mesenchymal transition and inhibitors of said targets
GB201319878D0 (en) * 2013-11-11 2013-12-25 Immunovia Ab Method, Array and use thereof
JP2017505301A (en) * 2014-01-08 2017-02-16 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル Methods and pharmaceutical compositions for preventing or reducing metastatic seeding
AU2015231053B2 (en) 2014-03-21 2019-04-11 Les Laboratoires Servier Compounds and their methods of use
JP5967161B2 (en) * 2014-09-17 2016-08-10 Jsr株式会社 Screening method for substances that affect epithelial maintenance of cells
US9921230B2 (en) * 2014-10-08 2018-03-20 Rhode Island Hospital Methods for diagnosis and treatment of concussion or brain injury
CN110139656A (en) 2016-08-05 2019-08-16 纽约州州立大学研究基金会 The Keratin 17 of biomarker as bladder cancer
GR20160100569A (en) * 2016-11-04 2018-08-29 Πανεπιστημιο Πατρων Method for the flurometric detection and quantification of the carbonyl groups of proteins
CN110029192A (en) * 2019-03-11 2019-07-19 中国人民解放军军事科学院军事医学研究院 EMT dynamic monitoring system, including its EMT Dynamic Monitoring System and application
WO2020257648A1 (en) * 2019-06-20 2020-12-24 Fred Hutchinson Cancer Research Center Microlumenal targeting of cancer cells
CN114787373A (en) * 2019-07-25 2022-07-22 免疫苏醒公司 Method of measuring cell-mediated killing by effectors
GB202010970D0 (en) 2020-07-16 2020-09-02 Immunovia Ab Methods, arrays and uses thereof
CN113817797A (en) * 2021-08-31 2021-12-21 皖南医学院 Method for researching influence of lactic acid on lung cancer cell metastasis invasion capacity

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Also Published As

Publication number Publication date
US7951549B2 (en) 2011-05-31
JP2011516826A (en) 2011-05-26
WO2009111067A2 (en) 2009-09-11
EP2271941B1 (en) 2012-08-08
WO2009111067A9 (en) 2009-12-23
US20090226396A1 (en) 2009-09-10
EP2271941A2 (en) 2011-01-12

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