WO2010056509A3 - Collection tubes apparatus, systems, and methods - Google Patents

Collection tubes apparatus, systems, and methods Download PDF

Info

Publication number
WO2010056509A3
WO2010056509A3 PCT/US2009/062169 US2009062169W WO2010056509A3 WO 2010056509 A3 WO2010056509 A3 WO 2010056509A3 US 2009062169 W US2009062169 W US 2009062169W WO 2010056509 A3 WO2010056509 A3 WO 2010056509A3
Authority
WO
WIPO (PCT)
Prior art keywords
whole blood
methods
tube
separator substance
collection tubes
Prior art date
Application number
PCT/US2009/062169
Other languages
French (fr)
Other versions
WO2010056509A2 (en
Inventor
Jane Emerson
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Priority to CA2743830A priority Critical patent/CA2743830C/en
Priority to CN2009801481631A priority patent/CN102227497A/en
Priority to JP2011536375A priority patent/JP2012508578A/en
Priority to EP09826538A priority patent/EP2346983A4/en
Publication of WO2010056509A2 publication Critical patent/WO2010056509A2/en
Publication of WO2010056509A3 publication Critical patent/WO2010056509A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/14—Devices for taking samples of blood ; Measuring characteristics of blood in vivo, e.g. gas concentration within the blood, pH-value of blood
    • A61B5/1405—Devices for taking blood samples
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation

Abstract

Methods of producing collection tubes are presented. The methods include providing a separator substance that can rapidly polymerize in a short time to a desired hardness and disposing the separator substance within the lumen of the tube. The separator substance is formulated to have a density between an average density of a serum fraction of whole blood and a cell-containing fraction of whole blood, and to be flowable with whole blood. Upon centrifugation of a tube having blood, the separator substance forms a barrier between the whole blood fractions. The tube and barrier maintain stability of one or more analyte levels, including potassium and glucose, within 10% of their initial values before centrifugation for at least four days.
PCT/US2009/062169 2008-11-14 2009-10-27 Collection tubes apparatus, systems, and methods WO2010056509A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2743830A CA2743830C (en) 2008-11-14 2009-10-27 Collection tubes apparatus, systems, and methods
CN2009801481631A CN102227497A (en) 2008-11-14 2009-10-27 Collection tubes apparatus, systems, and methods
JP2011536375A JP2012508578A (en) 2008-11-14 2009-10-27 Collection tube apparatus, system and method
EP09826538A EP2346983A4 (en) 2008-11-14 2009-10-27 Collection tubes apparatus, systems, and methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/271,610 US7971730B2 (en) 2005-08-10 2008-11-14 Collection tubes apparatus, systems and methods
US12/271,610 2008-11-14

Publications (2)

Publication Number Publication Date
WO2010056509A2 WO2010056509A2 (en) 2010-05-20
WO2010056509A3 true WO2010056509A3 (en) 2010-08-19

Family

ID=42170633

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/062169 WO2010056509A2 (en) 2008-11-14 2009-10-27 Collection tubes apparatus, systems, and methods

Country Status (7)

Country Link
US (3) US7971730B2 (en)
EP (1) EP2346983A4 (en)
JP (1) JP2012508578A (en)
KR (1) KR20110103940A (en)
CN (1) CN102227497A (en)
CA (1) CA2743830C (en)
WO (1) WO2010056509A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7673758B2 (en) * 2005-08-10 2010-03-09 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
DE102011007779A1 (en) * 2011-04-20 2012-10-25 Robert Bosch Gmbh Mixing chamber, cartridge and method for mixing a first and second component
KR101392239B1 (en) 2012-09-14 2014-05-07 주식회사 티씨케이 Scale rod for Reflector of ingot growth device
EP2925853B1 (en) * 2012-11-30 2019-07-24 Rarecyte, Inc. System for collecting a target material
US9539570B2 (en) 2012-11-30 2017-01-10 Rarecyte, Inc. Apparatus, system, and method for collecting a target material
CN103480056A (en) * 2013-08-15 2014-01-01 窦杰 Technology and device for treating rheumatoid bone disease by using osteopathic system
JP6539262B2 (en) * 2013-11-26 2019-07-03 レアサイト インコーポレイテッド Device, system and method for collecting target substance
EP3015169B1 (en) 2014-10-28 2024-04-10 The Regents Of The University Of California Sample collection tube with curable polymer separator
LT3015168T (en) 2014-10-28 2017-10-25 The Regents Of The University Of California Composite sealants and plasma separators for blood collection tubes
DE202014010872U1 (en) 2014-10-28 2017-01-16 The Regents Of The University Of California Composite release agent for blood collection tubes
DE202014010805U1 (en) 2014-10-28 2016-10-10 The Regents Of The University Of California Curable polymer release agent
DK3047864T3 (en) 2015-01-22 2018-06-14 Univ California COMPOSITIONS AND METHODS FOR SEPARATION OF TROMBOCYTRY PLASMA
DE202015009321U1 (en) 2015-01-22 2017-04-19 The Regents Of The University Of California Compositions for separating platelet-rich plasma
EP3247419B8 (en) 2015-01-22 2021-08-25 The Regents of the University of California Platelet rich plasma and bone marrow aspirate cell separation and removal methods and devices
KR102346470B1 (en) * 2015-03-31 2021-12-31 세키스이 메디칼 가부시키가이샤 Composition for separation of serum or plasma, and a blood collection container
CN110753843B (en) 2017-12-27 2022-11-04 积水医疗株式会社 Composition for separating serum or plasma, blood collection container, and method for separating serum or plasma
CN110873657B (en) * 2018-08-31 2022-02-08 北京致雨生物科技有限公司 Sampling method of micro-sample
CN114026026A (en) * 2019-03-14 2022-02-08 生米公司 Vial cover for biological treatment or analysis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4190535A (en) * 1978-02-27 1980-02-26 Corning Glass Works Means for separating lymphocytes and monocytes from anticoagulated blood
US4350593A (en) * 1977-12-19 1982-09-21 Becton, Dickinson And Company Assembly, compositions and method for separating blood
EP0350851A1 (en) * 1988-07-12 1990-01-17 Becton, Dickinson and Company Method and apparatus for separating mononuclear cells from blood
US20020146677A1 (en) * 2000-11-08 2002-10-10 Becton Dickinson And Company And Qiagen Gmbh. Method and device for collecting and stabilizing a biological sample

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3647070A (en) 1970-06-11 1972-03-07 Technicon Corp Method and apparatus for the provision of fluid interface barriers
US3780935A (en) 1972-07-10 1973-12-25 Lukacs & Jacoby Ass Serum separating method
US3920557A (en) 1974-02-27 1975-11-18 Becton Dickinson Co Serum/plasma separator--beads-plus-adhesive type
US3920549A (en) 1974-03-18 1975-11-18 Corning Glass Works Method and apparatus for multiphase fluid collection and separation
US3976579A (en) 1975-07-10 1976-08-24 Becton, Dickinson And Company Novel assembly
US4052320A (en) 1975-08-29 1977-10-04 Eastman Kodak Company Telescoping serum separator and dispenser
US4050451A (en) 1976-08-13 1977-09-27 Eastman Kodak Company Blood collection and separation device
US4101422A (en) 1977-05-11 1978-07-18 Emery Industries, Inc. Copolyesters useful in blood separation assemblies
US4235725A (en) 1978-08-16 1980-11-25 Owens-Illinois, Inc. Sterile blood-collecting and separating device
US4569764A (en) 1979-04-20 1986-02-11 Sherwood Medical Company Collection device with phase partitioning means
US4295974A (en) 1980-05-05 1981-10-20 Sherwood Medical Industries Inc. Blood sample collection and phase separation device
DE3165646D1 (en) 1980-05-08 1984-09-27 Terumo Corp Apparatus for separating blood
US4417981A (en) 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
US4386003A (en) 1981-09-17 1983-05-31 Sherwood Medical Industries Inc. Blood separating composition
US4460675A (en) 1982-01-21 1984-07-17 E. I. Du Pont De Nemours And Company Process for preparing an overcoated photopolymer printing plate
US4818418A (en) 1984-09-24 1989-04-04 Becton Dickinson And Company Blood partitioning method
US4751001A (en) 1984-09-24 1988-06-14 Becton Dickinson And Company Blood partitioning apparatus
US4640785A (en) 1984-12-24 1987-02-03 Becton Dickinson And Company Separation of lymphocytes and monocytes from blood samples
US4844818A (en) 1987-10-23 1989-07-04 Becton Dickinson & Company Method for separating the cellular components of blood samples
US4946601A (en) 1988-08-22 1990-08-07 Sherwood Medical Company Blood serum separator tube
US4894315A (en) 1988-08-30 1990-01-16 E. I. Du Pont De Nemours And Company Process for making flexographic printing plates with increased flexibility
US4994393A (en) 1989-02-22 1991-02-19 Becton, Dickinson And Company Blood partitioning composition
US5124434A (en) 1989-12-04 1992-06-23 Henkel Corporation Polyesters for use in blood partitioning compositions
US5266199A (en) 1990-11-20 1993-11-30 Nigata Chemicals And Plastics Co., Ltd. Serum separating apparatus
JP3251614B2 (en) 1991-03-29 2002-01-28 株式会社東芝 Polysilane
US5269927A (en) 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
JP2582191B2 (en) 1991-06-25 1997-02-19 株式会社ニッショー Gel-like material
JP2550232B2 (en) 1991-06-25 1996-11-06 株式会社ニッショー Blood separating agent
US5505853A (en) 1991-07-05 1996-04-09 Toyo Ink Manufacturing Co., Ltd. Serum:plasma separator and tube for the separation of serum:plasma from clot:hemocyte
JP3063799B2 (en) 1991-10-16 2000-07-12 株式会社ニッショー Blood separation agent
EP0624261B1 (en) 1992-01-31 1998-09-16 E.I. Du Pont De Nemours And Company Process for preparing photohardenable elastomeric element having increased exposure latitude
AU649828B2 (en) 1992-04-20 1994-06-02 Sekisui Chemical Co., Ltd. Serum and plasma separating compositions and blood testing containers
US5494590A (en) 1992-06-11 1996-02-27 Becton Dickinson Method of using anticoagulant solution in blood separation
AU7472994A (en) 1993-07-30 1995-02-28 Henkel Corporation Polyesters for use in blood partitioning
US5489386A (en) 1994-01-31 1996-02-06 Applied Imaging Density gradient medium for the separation of cells
US5518615A (en) 1994-04-22 1996-05-21 Becton, Dickinson And Company Blood compatible, shear sensitive gels
DE69534647T2 (en) 1994-09-19 2006-07-27 Sekisui Kagaku Kogyo K.K. CONTAINERS AND CARRIER FOR THE BLOOD TEST CONTAINING A BLOOD COMPONENT ADHESION INHIBITOR
TW404835B (en) 1994-10-05 2000-09-11 Becton Dickinson Co A viscoelastic dispersion composition
US5560830A (en) 1994-12-13 1996-10-01 Coleman; Charles M. Separator float and tubular body for blood collection and separation and method of use thereof
JPH08178236A (en) 1994-12-28 1996-07-12 Sekisui Plastics Co Ltd Resin combustion furnace and combustion control method thereof
AU707697B2 (en) 1995-08-28 1999-07-15 Sekisui Kagaku Kogyo Kabushiki Kaisha Serum or Plasma Separating Compositions
EP0766973A1 (en) 1995-09-29 1997-04-09 Becton, Dickinson and Company Blood collection device for plasma separation and method therefor
US6238578B1 (en) 1996-12-09 2001-05-29 Sherwood Services Ag Method for dispensing separator gel in a blood collection tube
JP3481030B2 (en) 1995-12-20 2003-12-22 三菱化学株式会社 Method for producing blood separation agent
US5731391A (en) 1996-09-27 1998-03-24 Henkel Corporation Fluid composition for physiological separations with enhanced resistance to inward migration of substances requiring accurate dosage monitoring
US5986039A (en) 1996-10-29 1999-11-16 Henkel Corporation Branched polyesters prepared from hydroxyfunctional components with functionality in excess of two and their use in physiological separation vehicles
US5906744A (en) 1997-04-30 1999-05-25 Becton Dickinson And Company Tube for preparing a plasma specimen for diagnostic assays and method of making thereof
US6979307B2 (en) 1997-06-24 2005-12-27 Cascade Medical Enterprises Llc Systems and methods for preparing autologous fibrin glue
ES2190230T3 (en) 1998-06-11 2003-07-16 Dow Global Technologies Inc PHOTOCURABLE ELASTOMER POLYMERS.
US6153113A (en) 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US6248844B1 (en) 1999-06-04 2001-06-19 Henkel Corporation Method of partitioning blood using polyesters
US6072022A (en) 1999-11-03 2000-06-06 Henkel Corporation Physiological fluid separation vehicles having improved resistance to inward migration of therapeutic agents
CA2430101C (en) 2000-11-28 2009-05-26 Asahi Kasei Kabushiki Kaisha Improved photosensitive resin for flexographic printing developable with an aqueous developer solution
DE10117234A1 (en) * 2001-04-06 2002-10-10 Alvito Biotechnologie Gmbh Porous and non-porous matrices based on chitosan and hydroxycarboxylic acids
US20030087178A1 (en) 2001-04-20 2003-05-08 Adrian Lungu Photopolymerizable element for use as a flexographic printing plate and a process for preparing the plate from the element
CN100456036C (en) 2001-12-04 2009-01-28 积水化学工业株式会社 Composition for blood serum or plasma separation and vessel for blood examination containing the same
AU2003279946A1 (en) 2002-10-10 2004-05-04 Becton Dickinson And Company Sample collection system with caspase inhibitor
WO2004073864A2 (en) 2003-02-13 2004-09-02 Becton, Dickinson And Company Devices for component removal during blood collection, and uses thereof
WO2005011495A1 (en) 2003-07-18 2005-02-10 Sekisui Chemical Co., Ltd. Hermetically sealed container and vacuum test substance-collecting container
KR100566124B1 (en) 2003-09-25 2006-03-30 레져 인코포레이티드 Apparatus for separating biological sample and separating method of the same
ATE396718T1 (en) 2003-12-19 2008-06-15 Rigel Pharmaceuticals Inc COMPOSITIONS AND METHODS FOR TREATING HEPATITIS C VIRUS (HCV) INFECTION
US8377461B2 (en) 2004-12-06 2013-02-19 Surmodics, Inc. Multifunctional medical articles
KR100776516B1 (en) 2005-04-12 2007-11-28 주식회사 엘지화학 Circuit for controlling Wake up of system having battery pack
US20070005024A1 (en) 2005-06-10 2007-01-04 Jan Weber Medical devices having superhydrophobic surfaces, superhydrophilic surfaces, or both
US7673758B2 (en) * 2005-08-10 2010-03-09 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
US7775962B2 (en) * 2005-08-10 2010-08-17 The Regents Of The University Of California Centrifuge with polymerizing energy source
US7674388B2 (en) 2005-08-10 2010-03-09 The Regents Of The University Of California Photopolymer serum separator
KR20060073446A (en) 2005-11-30 2006-06-28 주식회사 비즈모델라인 Method for lending deposit
ZA200602094B (en) * 2006-01-16 2007-11-28 Reliance Life Sciences Pvt Ltd Device for culturing and transporting cells
CN101454665A (en) 2006-05-25 2009-06-10 积水化学工业株式会社 Composition for separation of serum or plasma and container for blood test
WO2009085355A2 (en) 2007-10-01 2009-07-09 Longhorn Vaccines & Diagnostics Llc Biological specimen collection and transport system and methods of use

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4350593A (en) * 1977-12-19 1982-09-21 Becton, Dickinson And Company Assembly, compositions and method for separating blood
US4190535A (en) * 1978-02-27 1980-02-26 Corning Glass Works Means for separating lymphocytes and monocytes from anticoagulated blood
EP0350851A1 (en) * 1988-07-12 1990-01-17 Becton, Dickinson and Company Method and apparatus for separating mononuclear cells from blood
US20020146677A1 (en) * 2000-11-08 2002-10-10 Becton Dickinson And Company And Qiagen Gmbh. Method and device for collecting and stabilizing a biological sample

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2346983A4 *

Also Published As

Publication number Publication date
CN102227497A (en) 2011-10-26
KR20110103940A (en) 2011-09-21
EP2346983A2 (en) 2011-07-27
US8936162B2 (en) 2015-01-20
US20150027957A1 (en) 2015-01-29
CA2743830A1 (en) 2010-05-20
EP2346983A4 (en) 2012-08-08
US7971730B2 (en) 2011-07-05
JP2012508578A (en) 2012-04-12
US20110262322A1 (en) 2011-10-27
CA2743830C (en) 2017-03-07
US20090129973A1 (en) 2009-05-21
US9586203B2 (en) 2017-03-07
WO2010056509A2 (en) 2010-05-20

Similar Documents

Publication Publication Date Title
WO2010056509A3 (en) Collection tubes apparatus, systems, and methods
Fulton et al. Novel molecular determinants of viral susceptibility and resistance in the lipidome of E miliania huxleyi
WO2007126758A3 (en) Method and system for determining whether a drug will be effective on a patient with a disease
WO2008008515A3 (en) Methods and compositions for detecting rare cells from a biological sample
WO2007065025A3 (en) Method of dna analysis using micro/nanochannel
WO2010075061A3 (en) Processing blood
EP2186877A3 (en) Cytokine concentration system
WO2010141075A8 (en) Specific analysis of ketone and aldehyde analytes using reagent compounds, labeling strategies, and mass spectrometry workflow
WO2008118392A3 (en) Synthetic cell platforms and methods of use thereof
AR054427A1 (en) TREATMENT OF OSEOS DISORDERS
Zha et al. Immunomodulatory effects of a 3.2 kDa polypeptide from velvet antler of Cervus nippon Temminck
WO2008079169A3 (en) Nanonozzle device arrays: their preparation and use for macromolecular analysis
Juopperi et al. Isolation of bone marrow–derived stem cells using density-gradient separation
WO2012145414A3 (en) Apparatus and method for separating and concentrating fluids containing multiple components
Medepalli et al. Evaluation of the direct and indirect response of blood leukocytes to carbon nanotubes (CNTs)
EP1798293A3 (en) Gene set used for examination of colon cancer
WO2009125303A3 (en) Method and apparatus for determining a probability of colorectal cancer in a subject
EP4321135A3 (en) Easy empty urine collection bags
CN107795305A (en) A kind of method that regulation and control reservoir endogenous micro-organisms diversity improves recovery ratio
CN103558392B (en) A kind of effect of the antiatherosclerotic based on inflammatory reaction evaluation method
WO2009071892A3 (en) Method for the detection of dead and dying cells
de Hoog et al. Magnusiomyces Zender (1977)
WO2009062160A3 (en) Rantes levels as a diagnostic and therapeutic for acute graft versus host disease
EP1815856A3 (en) A cancer vaccine
Brockus et al. Disseminated Oxyporus corticola infection in a German shepherd dog

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200980148163.1

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09826538

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2011536375

Country of ref document: JP

Ref document number: 2009826538

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2743830

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 20117012262

Country of ref document: KR

Kind code of ref document: A