Useful fragments such as hyphae are extracted from a raw sample containing water such a fungi attached to a plant. The sample is rapidly frozen and cut under vacuum largely and at random. Water contained in the sample is sublimated and the sample is coated with conductive material. While observing the...http://www.google.com/patents/US4749868?utm_source=gb-gplus-sharePatent US4749868 - Method of processing a sample containing water under scanning electron microscope and apparatus thereof
