Plant protoplasts are exposed for interaction with an external agent by exposing the plasma membrane of protoplasts in the root hairs of the plant without release of protoplasts from the plant. This may be achieved by enzymatically degrading the plant cell wall at the apices of root hairs under conditions...http://www.google.com/patents/US5126263?utm_source=gb-gplus-sharePatent US5126263 - Utilization of plant protoplasts