SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an inoculum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree. C while continuing the agitation of the medium,...http://www.google.com/patents/US4059486?utm_source=gb-gplus-sharePatent US4059486 - Cell culture process
