(19) United States
(12) Patent Application Publication (io) Pub. No.: US 2004/0241666 Al
Amorese et al. (43) Pub. Date: Dec. 2,2004
(54) LIGAND ARRAY ASSAYS THAT INCLUDE AN ORGANIC FLUID WASH STEP AND COMPOSITIONS FOR PRACTICING THE SAME
(76) Inventors: Douglas A. Amorese, Los Altos, CA
(US); Eric M. Leproust, San Jose, CA
(US); Winny W. Ke, San Jose, CA
AGILENT TECHNOLOGIES, INC.
ADMINISTRATION, LEGAL DEPT.
P.O. BOX 7599
LOVELAND, CO 80537-0599 (US) (21) Appl. No.: 10/452,767
(22) Filed: May 30, 2003
(51) Int. CI.7 C12Q 1/68; C12M 1/34
(52) U.S. CI 435/6; 435/287.2
Ligand array assays and compositions for use in practicing the same are provided. A feature of the subject methods is that they include a wash step in which the ligand displaying surface of a sample exposed ligand array is washed with organic wash fluid, e.g., propylene carbonate. Also provided are kits for use in practicing the subject methods. The subject methods and kits find use in a variety of ligand array based applications, including genomic and proteomic applications.