(54) HIGH EFFICIENCY CELL ANALYSIS
SYSTEM AND HIGH THROUGHPUT DRUG
(75) Inventor: Gary Brooker, Rockville, MD (US)
(73) Assignee: Atto Instruments, LLC, Rockville, MD (US)
( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days.
(21) Appl. No.: 09/886,102
(22) Filed: Jun. 22, 2001
(65) Prior Publication Data
US 2002/0031789 Al Mar. 14, 2002
Related U.S. Application Data
(60) Provisional application No. 60/213,179, filed on Jun. 22, 2000.
(51) Int. C I. C12Q 1 00
(52) U.S. CI 435/4; 435/30; 435/286.4;
435/287.3; 435/288.4; 435/288.7; 356/904
(58) Field of Search 422/62, 82.05,
422/4, 7.1; 435/30, 286.2, 286.4, 287.3, 288.7, 288.4; 356/904
(56) References Cited
U.S. PATENT DOCUMENTS
5,804,436 A 9/1998 Okun et al 435/286.1
A system which can analyze compounds with high efficiency, such as in a high throughput drug screening system. The high throughput drug screening system which can test the action of a drug candidate upon a group of cells in a monolayer. A microspace corresponding to a microscopic field area, for example on the order of 100-200 microns in diameter, is isolated from the other cells on the monolayer by creating a seal between a drug delivery perfusion unit and the cells to create the microspace for analysis. A drug candidate is then provided into the isolated microspace. The interaction between the drug candidate and the cells in the isolated microspace can then be evaluated. With the high throughput drug screening system the vast majority of cells on a monolayer can be used for drug testing. The high throughput drug system also makes it more readily available to use primary cells in addition to immortal cells as the cell layer.
23 Claims, 12 Drawing Sheets